Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F3 I0 R1
|
560 |
56.4 |
4650532 |
94.9% |
4413354 |
59.3 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
W3110S.gb |
3,602,757 |
T→A |
21.9% |
L260F (TTA→TTT) |
trkH ← |
potassium transporter |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 3,602,757 | 0 | T | A | 21.9%
| 130.8
/ 35.7
| 64 | L260F (TTA→TTT) | trkH | potassium transporter |
| Reads supporting (aligned to +/- strand): ref base T (28/22); new base A (8/6); total (36/28) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.47e-01 |
AACTGTACGCCGATAAACATGCGAAATTCCGGATCGCGCCAATAAACCTTCAGACTACGCCCACTTAACAGTGAAAAGTGCAGACCGTAGTTACAGCCGGAGATCAGCAGGAAGATAGCAATGATAGTGTTAA > W3110S.gb/3602692‑3602824
|
aaCTGTACGCCGATAAACATGCGAAATTCCGGATCGCGCCAATAAACCTTCAGACTACGCCCACTTAACAg > 1:3090583/1‑71 (MQ=255)
aaCTGTACGCCGATAAACATGCGAAATTCCGGATCGCGCCAATAAACCTTCAGACTACGCCCACTAAACAg > 1:2516926/1‑71 (MQ=255)
aaCTGTACGCCGATAAACATGCGAAATTCCGGATCGCGCCAATAAACCTTCAGACTACGCCCACTAAACAg > 1:1955165/1‑71 (MQ=255)
tACGCCGATAAACATGCGAAATTCCGGATCGCGCCAATAAACCTTCAGACTACGCCCACTTAACAGTGaa < 1:1390253/70‑1 (MQ=255)
tACGCCGATAAACATGCGAAATTCCGGATCGCGCCAATAAACCTTCAGACTACGCCCACTAAACAGTGaa > 1:1488456/1‑70 (MQ=255)
cGCCGATAAACATGCGAAATTCCGGATCGCGCCAATAAACCTTCAGACTACGCCCACTTa > 1:3481297/1‑60 (MQ=255)
cGCCGATAAACATGCGAAATTCCGGATCGCGCCAATAAACCTTCAGACTACGCCCACTAAACAGTGAAAAg < 1:621437/71‑1 (MQ=255)
gCCGATAAACATGCGAAATTCCGGATCGCGCCAATAAACCTTCAGACTACGCCCACTAAACAGTGAAAAGt < 1:117587/71‑1 (MQ=255)
ccGATAAACATGCGAAATTCCGGATCGCGCCAATAAACCTTCAGACTACGCCCACTTAACAGTGaaa < 1:1513989/67‑1 (MQ=255)
gATAAACATGCGAAATTCAGGATCGCGCCAATAAACCTTCAGACTACGCCCACTTAACAGTGAAAAGTg > 1:2200486/1‑69 (MQ=255)
aTAAACATGCGAAATTCCGGATCGCGCCAATAAACCTTCAGACTACGCCCACTTAACAGTGAAAAGTg > 1:3741828/1‑68 (MQ=255)
aTAAACATGCGAAATTCCGGATCGCGCCAATAAACCTTCAGACTACGCCCACTTAACAGTGAAAAGTg > 1:2754876/1‑68 (MQ=255)
aTAAACATGCGAAATTCCGGATCGCGCCAATAAACCTTCAGACTACGCCCACTAAACAGTGAAAAg < 1:4305064/66‑1 (MQ=255)
aTAAAAATGCGAAATTCCGGATCGCGCCAATAAACCTTCAGACTACGCCCACTTAACAGTGAAAAGTg > 1:4515383/1‑68 (MQ=255)
tAAACATGCGAAATTCCGGATCGCGCCAATAAACCTTCAGACTACGCCCACTTAACAGTGAAAAGTGCAGa > 1:2877061/1‑71 (MQ=255)
aaaCATGCGAAATTCCGGATCGCGCCAATAAACCTTCAGACTACGCCCACTTAACAGTGAAAAGTGCAGAc > 1:3974648/1‑71 (MQ=255)
cATGCGAAATTCCGGATCGCGCCAATAAACCTTCAGACTACGCCCACTTAACAGTGAAAAGTGCAGACCGt < 1:4386222/71‑1 (MQ=255)
aTGCGAAATTCCGGATCGCGCCAATAAACCTTCAGACTACGCCCACTTAACAGTGAAAAGTGCAGACCg > 1:1984696/1‑69 (MQ=255)
aTGCGAAATTCCGGATCGCGCCAATAAACCTTCAGACTACGCCCACTTAACAGTGAAAAGTGCAGACCg > 1:3415955/1‑69 (MQ=255)
gCGAAATTCCGGATCGCGCCAATAAACCTTCAGACTACGCCCACTAAACAGTGAAAAGTGCAGACCGTAGt > 1:1913404/1‑71 (MQ=255)
cGAAATTCCGGATCGCGCCAATAAACCTTCAGACTACGCCCACTTAACAGTGAAAAGTg > 1:1665389/1‑59 (MQ=255)
agAAATTCCGGATCGCGCCAATAAACCTTCAGACTACGCCCACTTAACAGTGAAAAGTGCAGACCGTAGt > 1:3698853/2‑70 (MQ=255)
gAAATTCCGGATCGCGCCAATAAACCTTCAGACTACGCCCACTTAACAGTGAAAAGTGCAGACCGTAGTTa > 1:2377654/1‑71 (MQ=255)
aTTCCGGATCGCGCCAATAAACCTTCAGACTACGCCCACTTAACAGTGaa < 1:2276192/50‑1 (MQ=255)
aTTCCGGATCGCGCCAATAAACCTTCAGACTACGCCCACTTAACAGTGaa < 1:785670/50‑1 (MQ=255)
aTTCCGGATCGCGCCAATAAACCTTCAGACTACGCCCACTTAACAGTGAAAAGTGCa > 1:2351225/1‑57 (MQ=255)
aTTCCGGATCGCGCCAATAAACCTTCAGACTACGCCCACTTAACAGTGAAAAGTGCAGACCGTAGTTACAg > 1:2878118/1‑71 (MQ=255)
aTTCCGGATCGCGCCAATAAACCTTCAGACTACGCCCACTAAACAGTGAAAAGTGCAGACCGTAGTTACAg > 1:4619946/1‑71 (MQ=255)
ttCCGGATCGCGCCAATAAACCTTCAGACTACGCCCACTTAACAGTGaaa < 1:293759/50‑1 (MQ=255)
ttCCGGATCGCGCCAATAAACCTTCAGACTACGCCCACTTAACAGTGaa < 1:2129091/49‑1 (MQ=255)
ttCCGGATCGCGCCAATAAACCTTCAGACTACGCCCACTTAACAGTGAAAAGTGCAGACCGTAGTTACAGc < 1:3570194/71‑1 (MQ=255)
ttCCGGATCGCGCCAATAAACCTTCAGACTACGCCCACTTAACAGTGAAAAGTGCAGACCGTAGTTACAGc < 1:3551252/71‑1 (MQ=255)
tCCGGATCGCGCCAATAAACCTTCAGACTACGCCCACTTAACAGTGAAAAGTg > 1:1990299/1‑53 (MQ=255)
tCCGGATCGCGCCAATAAACCTTCAGACTACGCCCACTTAACAGTGAAAAGTg > 1:1309334/1‑53 (MQ=255)
ccGGATCGCGCCAATAAACCTTAAGACTACGCCAACTAAAc > 1:69660/1‑41 (MQ=25)
cGGATCGCGCCAATAAACCTTCAGACTACGCCCACTTAACAGTGaaaa > 1:3814053/1‑48 (MQ=255)
cGGATCGCGCCAATAAACCTTCAGACTACGCCCACTTAACAGTGaaaa > 1:2000430/1‑48 (MQ=255)
ggATCGCGCCAATAAACCTTCAGACTACGCCCACtt < 1:3576878/36‑1 (MQ=255)
ggATCGCGCCAATAAACCTTCAGACTACGCCCACTTAACAGTGAAAAGTGCAGACCGTAGTTACAGCCg > 1:2115701/1‑69 (MQ=255)
gATCGCGCCAATAAACCTTCAGACTACGCCCACTAAACAGTGAAAAGTGCAGACCGTAGTTACAGCCGgag < 1:1890008/71‑1 (MQ=255)
aTCGCGCCAATAAACCTTCAGACTACGCCCACTTAACAGTGaa < 1:2217228/43‑1 (MQ=255)
aTCGCGCCAATAAACCTTCAGACTACGCCCACTTAACAGTGAAAAGTGCAGa > 1:2672301/1‑52 (MQ=255)
aTCGCGCCAATAAACCTTCAGACTACGCCCACTAAACAGTGAAAAGTGCAGACCGTAGTTAAAg > 1:3763681/1‑64 (MQ=255)
tCGCGCCAATAAACCTTCAGACTACGCCCACTTAACAGTGAAAAGTGCAGACCGTAGTTACAGCCGgaga < 1:2840431/70‑1 (MQ=255)
cgcgCCAATAAACCTTCAGACTACGCCCACTTAACAGTGAAAAGTGCAGACCGTAGTTACAGCCGGAGAt > 1:2966394/1‑70 (MQ=255)
ccAATAAACCTTCAGACTACGCCCACTTAACAGTGAAAAGTGCAGACCGTAGt > 1:4127132/1‑53 (MQ=255)
aaaCCTTCAGACTACGCCCACTTAACAGTGAAAAGTg < 1:1866978/37‑1 (MQ=255)
aaCCTTCAGACTACGCCCACTTAACAGTGAAAAGTGCAGACCGTAGTTAc < 1:1915430/50‑1 (MQ=255)
aaCCTTCAGACTACGCCCACTTAACAGTGAAAAGTGCAGACCGTAGTTAc < 1:1920834/50‑1 (MQ=255)
aaCCTTCAGACTACGCCCACTTAACAGTGAAAAGTGCAGACCGTAGTTACAGCCGGAGATCAGCAgg > 1:1119980/1‑67 (MQ=255)
aaCCTTCAGACTACGCCCACTAAACAGTGAAAAGTGCAGACCGTAGTTACAGCCGGAGATCAGCAGGAAg < 1:974222/70‑1 (MQ=255)
aCCTTCAGACTACGCCCACTAAACAGTGAAAAGTg < 1:4184436/35‑1 (MQ=255)
cTTCAGACTACGCCCACTTAACAGTGAAAAGTGCAGACCGTAGTTACAGCCGGAGATCAGCAGGAAGATAg > 1:3435503/1‑71 (MQ=255)
cAGACTACGCCCACTTAACAGTGAAAAGTGCAGACCGTAGTTACAGCCGGAGATCAGCAGGAAGATAGCaa > 1:3472095/1‑71 (MQ=255)
cAGACTACGCCCACTTAACAGTGAAAAGTGCAGACCGTAGTTACAGCCGGAGATCAGCAGGAAGATAGCa < 1:4153230/70‑1 (MQ=255)
gACTACGCCCACTTAACAGTGAAAAGTGCAGACCGTAGTTACAGCCGgag < 1:3081314/50‑1 (MQ=255)
aCTACGCCCACTTAACAGTGAAAAGTGCAGACCGTAGt < 1:4152088/38‑1 (MQ=255)
aCTACGCCCACTTAACAGTGAAAAGTGCAGACCGTAGTTACAGCCGGAGATCAGCAGGAAGATAGCAATGa < 1:3939404/71‑1 (MQ=255)
cTACGCCCACTTAACAGTGAAAAGTGCAGACCGTAGTTACAGCCGgaga > 1:3126349/1‑49 (MQ=255)
cACTTAACAGTGAAAAGTGCAGACCGTAGTTACAGCCGgag < 1:482569/41‑1 (MQ=255)
cACTTAACAGTGAAAAGTGCAGACCGTAGTTACAGCCGGAGATCAGCAGGAAGATAGCAATGATAGTGtt > 1:4326160/1‑70 (MQ=255)
cACTTAACAGTGAAAAGTGCAGACCGTAGTTACAGCCGGAGATCAGCAGGAAGATAGCAATGATAGTGTTa < 1:4441328/71‑1 (MQ=255)
cACTTAACAGTGAAAAGTGCAGACCGTAGTTACAGCCGGAGATCAGCAGGAAGATAGCAATGATAGTGTTa < 1:2337145/71‑1 (MQ=255)
aCTAAACAGTGAAAAGTGCAGACCGTAGTTACAGCCGGAGATCAGCAGGAAGATAGCAATGATa > 1:2885531/1‑64 (MQ=255)
cTTAACAGTGAAAAGTGCAGACCGTAGTTACAGCCGGAGATCAGCAGGAAGATAGCAATGATAGTGtt < 1:1890182/68‑1 (MQ=255)
ttAACAGTGAAAACTGCAGACCGTAGTTACAGCCGGAGATCAGCAGGAAGATAGCAATGATAGTGtt < 1:4404040/67‑1 (MQ=255)
tAACAGTGAAAAGTGCAGACCGTAGTTACAGCCGGAGATCAGCAGGAAGATAGCAATGATAGTGTTaa > 1:4363388/1‑68 (MQ=255)
|
AACTGTACGCCGATAAACATGCGAAATTCCGGATCGCGCCAATAAACCTTCAGACTACGCCCACTTAACAGTGAAAAGTGCAGACCGTAGTTACAGCCGGAGATCAGCAGGAAGATAGCAATGATAGTGTTAA > W3110S.gb/3602692‑3602824
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
