Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F8 I0 R1
|
311 |
50.1 |
4085234 |
95.9% |
3917739 |
60.7 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
W3110S.gb |
3,602,757 |
T→A |
31.3% |
L260F (TTA→TTT) |
trkH ← |
potassium transporter |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 3,602,757 | 0 | T | A | 31.3%
| 82.4
/ 57.3
| 64 | L260F (TTA→TTT) | trkH | potassium transporter |
| Reads supporting (aligned to +/- strand): ref base T (21/23); new base A (8/12); total (29/35) |
| Fisher's exact test for biased strand distribution p-value = 6.00e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.79e-01 |
GCGTAAACTGTACGCCGATAAACATGCGAAATTCCGGATCGCGCCAATAAACCTTCAGACTACGCCCACTTAACAGTGAAAAGTGCAGACCGTAGTTACAGCCGGAGATCAGCAGGAAGATAGCAATGATAGTGTTAATAG > W3110S.gb/3602687‑3602827
|
gCGTAAACTGTACGCCGATAAACATGCGAAATTCCGGATCGCGCCAATAAACCTTCAGACTACGCCCACtt < 1:1094108/71‑1 (MQ=255)
cGTAAACTGTACGCCGATAAACATGCGAAATTCCGGATCGCGCCAATAAACCTTCAGACTACGCCCACtt < 1:311678/70‑1 (MQ=255)
tAAACTGTACGCCGATAAACATGCGAAATTCCGGATCGCGCCAATAAACCTTCAGACTACGCCCACtt < 1:3285115/68‑1 (MQ=255)
tAAACTGTACGCCGATAAACATGCGAAATTCCGGATCGCGCCAATAAACCTTCAGACTACGCCCACtt < 1:1161651/68‑1 (MQ=255)
aaCTGTACGCCGATAAACATGCGAAATTCCGGATCGCGCCAATAAACCTTCAGACTACGCCCACTTAACa > 1:1073069/1‑70 (MQ=255)
aaCTGTACGCCGATAAACATGCGAAATTCCGGATCGCGCCAATAAACCTTCAGACTACGCCCACTTAACAg > 1:415674/1‑71 (MQ=255)
tcTGTACGCCGATAAACATGCGAAATTCCGGATCGCGCCAATAAACCTTCAGACTACGCCCACTTAACa < 1:2829112/68‑1 (MQ=255)
tACGCCGATAAACATGCGAAATTCCGGATCGCGCCAATAAACCTTCAGACTACGCCCACTTaa > 1:320427/1‑63 (MQ=255)
tACGCCGATAAACATGCGAAATTCCGGATCGCGCCAATAAACCTTCAGACTACGCCCACTTaa > 1:3052586/1‑63 (MQ=255)
cGCCGATAAACATGCGAAATTCCGGATCGCGCCAATAAACCTTCAGACTACGCCCACTTAACa > 1:2746753/1‑63 (MQ=255)
cGCCGATAAACATGCGAAATTCCGGATCGCGCCAATAAACCTTCAGACTACGCCCACTTAACa > 1:2332657/1‑63 (MQ=255)
cGCCGATAAACATGCGAAATTCCGGATCGCGCCAATAAACCTTCAGACTACGCCCACTTAACAGTGAAAAg > 1:980109/1‑71 (MQ=255)
cGCCGATAAACATGCGAAATTCCGGATCGCGCCAATAAACCTTCAGACTACGCCCACTTAACAGTGAAAAg > 1:257588/1‑71 (MQ=255)
cGCCGATAAACATGCGAAATTCCGGATCGCGCCAATAAACCTTCAGACTACGCCCACTTAACAGTGAAAAg > 1:2031280/1‑71 (MQ=255)
ccGATAAACATGCGAAATTCCGGATCGCGCCAATAAACCTTCAGACTACGCCCACTTAACAGTGAAAAGtt < 1:3261516/71‑2 (MQ=255)
ccGATAAACATGCGAAATTCCGGATCGCGCCAATAAACCTTCAGACTACGCCCACTTAACAGTGAAAAGTg < 1:200269/71‑1 (MQ=255)
ccGATAAACATGCGAAATTCCGGATCGCGCCAATAAACCTTCAGACTACGCCCACTTAACAGTGAAAAGTg < 1:1348692/71‑1 (MQ=255)
cGATAAACATGCGAAATTCCGGATCGCGCCAATAAACCTTCAGACTACGCCCACTTAACAGTGAAAAGTg < 1:1771997/70‑1 (MQ=255)
cGATAAACATGCGAAATTCCGGATCGCGCCAATAAACCTTCAGACTACGCCCACTTAACAGTGAAAAGTg < 1:2537062/70‑1 (MQ=255)
gATAAACATGCGAAATTCCGGATCGCGCCAATAAACCTTCAGACTACGCCCACTTAACAGTGAAAAGTg < 1:1126035/69‑1 (MQ=255)
gATAAACATGCGAAATTCCGGATCGCGCCAATAAACCTTCAGACTACGCCCACTTAACAGTGAAAAGTg < 1:4022518/69‑1 (MQ=255)
aaaCATGCGAAATTCCGGATCGCGCCAATAAACCTTCAGACTACGCCCACTTAACAGTGAAAAGTGCAGAc > 1:2730956/1‑71 (MQ=255)
aTGCGAAATTCCGGATCGCGCCAATAAACCTTCAGACTACGCCCACTTAACAGTGAAAAGTGCAGACCGTa < 1:811044/71‑1 (MQ=255)
aaTTCCGGATCGCGCCAATAAACCTTCAGACTACGCCCACTTAACAGTGAAAAGTGCAGACCGTAg > 1:285543/1‑66 (MQ=255)
aaTTCCGGATCGCGCCAATAAACCTTCAGACTACGCCCACTTAACAGTGAAAAGTGCAGACCGTAg > 1:3801609/1‑66 (MQ=255)
aaTTCCGGATCGCGCCAATAAACCTTCAGACTACGCCCACTTAACAGTGAAAAGTGCAGACCGTAGTTACa < 1:2813700/71‑1 (MQ=255)
aaTTCCGGATCGCGCCAATAAACCTTCAGACTACGCCCACTTAACAGTGAAAAGTGCAGACCGTAGTTACa < 1:520206/71‑1 (MQ=255)
tCCGGATCGCGCCAATAAACCTTCAGACTACGCCCACTaaa > 1:3089910/1‑41 (MQ=255)
tCCGGATCGCGCCAATAAACCTTCAGACTACGCCCACTTaa > 1:1936238/1‑41 (MQ=255)
tCCGGATCGCGCCAATAAACCTTCAGACTACGCCCACTTaa > 1:1095420/1‑41 (MQ=255)
tCCGGATCGCGCCAATAAACCTTCAGACTACGCCCACTTa > 1:2024202/1‑40 (MQ=255)
ccGGATCGCGCCAATAAACCTTCAGACTACGCCCACTAAACAGTGAAAAGTGCAGACCGTAGTTACAGCCg > 1:701599/1‑71 (MQ=255)
gATCGCGCCAATAAACCTTCAGACTACGCCCACTAAACAGTGAAAAGTGCAGACCGTAGTTACAGCCGgag < 1:1106959/71‑1 (MQ=255)
aTCGCGCCAATAAACCTTCAGACTACGCCCACTAAACAGTGAAAAGTGCAGACCGTAGTTACAGCCGgag < 1:2888/70‑1 (MQ=255)
tCGCGCCAATAAACCTTCAGACTACGCCCACTTAACAGTGAAAAGTGCAGACCGTAGTTACAGCCGGAGAt < 1:795532/71‑1 (MQ=255)
ccAATAAACCTTCAGACTACGCCCACTTAACAGTGAAAAGTg < 1:3392117/42‑1 (MQ=255)
ccAATAAACCTTCAGACTACGCCCACTTAACAGTGAAAAGTg < 1:2343858/42‑1 (MQ=255)
ccAATAAACCTTCAGACTACGCCCACTTAACAGTGAAAAGTg < 1:1877418/42‑1 (MQ=255)
ccAATAAACCTTCAGACTACGCCCACTTAACAGTGAAAAGTg < 1:3063942/42‑1 (MQ=255)
aaTAAACCTTCAGACTACGCCCACTTAACAGTGAAAAGTGCa > 1:2871351/1‑42 (MQ=255)
aaTAAACCTTCAGACTACGCCCACTAAACAGTGGAAAGTGCAGACCGTAGTTACAGCCGGAGATcagcag > 1:3499311/1‑70 (MQ=255)
aaTAAACCTTCAGACTACGCCCACTAAACAGTGAAAAGTGCAGACCGTAGTTACAGCCGGAGATcagcag > 1:3625325/1‑70 (MQ=255)
aaTAAACCTTCAGACTACGCCCACTAAACAGTGAAAAGTGCAGACCGTAGTTACAGCCGGAGATcagcag > 1:419306/1‑70 (MQ=255)
aaTAAACCTTCAGACTACGCCCACTAAACAGTGAAAAGTGCAGACCGTAGTTACAGCCGGAGATcaccag > 1:3395442/1‑70 (MQ=255)
aTAAACCTTCAGACTACGCCCACTTAACAGTGaaaa < 1:3790531/36‑1 (MQ=255)
tAAACCTTCAGACTACGCCCACTTAACAGTGAAAAGTg < 1:2578968/38‑1 (MQ=255)
tAAACCTTCAGACTACGCCCACTTAACAGTGAAAAGTGCAGACCGTAGTTACAGCCGGAGATCAGCAGGaa < 1:2478114/71‑1 (MQ=255)
tAAACCTTCAGACTACGCCCACTAAACAGTGAAAAGTGCAGACCGTAGTTACAGCCGGAGATCAGCAGGaa < 1:2836058/71‑1 (MQ=255)
tAAACCTTCAGACTACGCCCACTAAACAGTGAAAAGTGCAGACCGTAGTTACAGCCGGAGATCAGCAGGaa < 1:364655/71‑1 (MQ=255)
tAAACCTTCAGACTACGCCCACTAAACAGTGAAAAGTGCAGACCGTAGTTACAGCCGGAGATCAGCAGGaa < 1:3580994/71‑1 (MQ=255)
aaCCTTCAGACTACGCCCACTTAACAGTGAAAAGTGCAGACCGTAGTTACAGCCGGAGATCAGCAgg > 1:2306806/1‑67 (MQ=255)
aaCCTTCAGACTACGCCCACTAAACAGTGAAAAGTGCAGACCGTAGTTACAGCCGGAGATCAGCAgg > 1:2500473/1‑67 (MQ=255)
aaCCTTCAGACTACGCCCACTAAACAGTGAAAAGTGCAGACCGTAGTTACAGCCGGAGATCAGCAgg > 1:2534000/1‑67 (MQ=255)
ttCAGACTACGCCCACTTAACAGTGAAAAGTGCAGACCGTAGTTACAGCCGGAGATCAGCAGGaa < 1:340653/65‑1 (MQ=255)
aGACTACGCCCACTAAACAGTGAAAAGTGCAGACCGTAGTTACAGCCGGAGATCAGCAGGAAGATAGCAAt < 1:3570258/71‑1 (MQ=255)
aGACTACGCCCACTAAACAGTGAAAAGTGCAGACCGTAGTTACAGCCGGAGATCAGCAGGAAGATAGCAAt < 1:3659422/71‑1 (MQ=255)
cTACGCCCACTTAACAGTGAAAAGTGCAGACCGTAGTTACAGCCGGAGATCAGCAGGAAGATAGCAATGAt < 1:164969/71‑1 (MQ=255)
ccACTTAACAGTGAAAAGTGCAGACCGTAGTTACAGCCGGAGATCAGCAGGAAGATAGCAATGATAGTGtt < 1:1053760/71‑1 (MQ=255)
ccACTTAACAGTGAAAAGTGCAGACCGTAGTTACAGCCGGAGATCAGCAGGAAGATAGCAATGATAGTGtt < 1:3222712/71‑1 (MQ=255)
ccACTAAACAGTGAAAAGTGCAGACCGTAGTTACAGCCGGAGATcagcag < 1:2820260/50‑1 (MQ=255)
ccACTAAACAGTGAAAAGTGCAGACCGTAGTTACAGCCGGAGATcagcag < 1:4003792/50‑1 (MQ=255)
ccACTAAACAGTGAAAAGTGCAGACCGTAGTTACAGCCGGAGATcagcag < 1:2371622/50‑1 (MQ=255)
ccACTAAACAGTGAAAAGTGCAGACCGTAGTTACAGCCGGAGATcagcag < 1:2197538/50‑1 (MQ=255)
ccACTAAACAGTGAAAAGTGCAGACCGTAGTTACAGCCGGAGATcagcag < 1:1795814/50‑1 (MQ=255)
cACTAAACAGTGAAAAGTGCAGACCGTAGTTACAGCCGGAGATcagca > 1:2541306/1‑48 (MQ=255)
cTTAACAGTGAAAAGTGCAGACCGTAGTTACAGCCGGAGATCAGCAGGAAGATAGCAATGa > 1:1256311/1‑61 (MQ=255)
cTTAACAGTGAAAAGTGCAGACCGTAGTTACAGCCGGAGATCAGCAGGAAGATAGCAATGa > 1:2505972/1‑61 (MQ=255)
cTTAACAGTGAAAAGTGCAGACCGTAGTTACAGCCGGAGATCAGCAGGAAGATAGCAATGATAGTGTTaa > 1:827220/1‑70 (MQ=255)
cTTAACAGTGAAAAGTCCAGACCGTAGTTACAGCCGGAGATCAGCAGGAAGATAGCAATGa > 1:2724716/1‑61 (MQ=255)
tAACAGTGAAAAGTGCAGACCGTAGTTACAGCCGGAGATcagca > 1:3368651/1‑44 (MQ=255)
tAACAGTGAAAAGTGCAGACCGTAGTTACAGCCGGAGATcagca > 1:3060631/1‑44 (MQ=255)
tAACAGTGAAAAGTGCAGACCGTAGTTACAGCCGGAGATcagca > 1:2124266/1‑44 (MQ=255)
tAACAGTGAAAAGTGCAGACCGTAGTTACAGCCGGAGATCAGCAGGAAGATAGCAATGATAGTGTTaa > 1:1734175/1‑68 (MQ=255)
tAACAGTGAAAAGTGCAGACCGTAGTTACAGCCGGAGATCAGCAGGAAGATAGCAATGATAGTGTTAATAg > 1:1225101/1‑71 (MQ=255)
|
GCGTAAACTGTACGCCGATAAACATGCGAAATTCCGGATCGCGCCAATAAACCTTCAGACTACGCCCACTTAACAGTGAAAAGTGCAGACCGTAGTTACAGCCGGAGATCAGCAGGAAGATAGCAATGATAGTGTTAATAG > W3110S.gb/3602687‑3602827
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
