Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F28 I2 R1
|
28 |
4.9 |
280516 |
80.6% |
226095 |
64.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
minE |
1,935,566 |
A→T |
intergenic (‑16/+75) |
prfB ← / ← recJ |
peptide chain release factor RF‑2/ssDNA exonuclease, 5' ‑‑> 3'‑specific |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 1,935,566 | 0 | A | T | 100.0%
| 10.8
/ NA
| 5 | intergenic (‑16/+75) | prfB/recJ | peptide chain release factor RF‑2/ssDNA exonuclease, 5' ‑‑> 3'‑specific |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base T (4/1); total (4/1) |
ATGCGATTATTTACCGGATTAATTTCAAACATGGTCTGATTTCTTTTATTGAGCTAGTCAAAATGCGGTGATAAGAGCGGGATTG > minE/1935519‑1935603
|
aTGCGATTATTTACCGGATTAATTTCAAACATGGTCTGATTTCTTTTTTTGAGCTAGTCAAAATGCGGTGa < 1:202800/71‑1 (MQ=255)
gTCTGATTTCTTTTTTTGAGCTAGTCAAAATGCGGTGATAAGAGCGGGAt > 1:168287/1‑50 (MQ=255)
tCTTTTTTTGAGCTAGTCAAAATGCGGTGATAAGAGCGGGATTg > 1:138326/1‑44 (MQ=255)
tCTTTTTTTGAGCTAGTCAAAATGCGGTGATAAGAGCGGGATTg > 1:167854/1‑44 (MQ=255)
tCTTTTTTTGAGCTAGTCAAAATGCGGTGATAAGAGCGGGATTg > 1:171693/1‑44 (MQ=255)
|
ATGCGATTATTTACCGGATTAATTTCAAACATGGTCTGATTTCTTTTATTGAGCTAGTCAAAATGCGGTGATAAGAGCGGGATTG > minE/1935519‑1935603
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
