Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F8 I0 R1
|
82 |
77.9 |
2158605 |
86.8% |
1873669 |
62.5 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
1,935,566 |
A→T |
100% |
intergenic (‑16/+75) |
prfB ← / ← recJ |
peptide chain release factor RF‑2/ssDNA exonuclease, 5' ‑‑> 3'‑specific |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 1,935,566 | 0 | A | T | 100.0%
| 37.8
/ NA
| 13 | intergenic (‑16/+75) | prfB/recJ | peptide chain release factor RF‑2/ssDNA exonuclease, 5' ‑‑> 3'‑specific |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base T (0/13); total (0/13) |
| Rejected as polymorphism: Frequency below/above cutoff threshold. |
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. |
| Rejected as polymorphism: Polymorphic base substitution creates a homopolymer stretch. |
TTAATTTCAAACATGGTCTGATTTCTTTTATTGAGCTAGTC > minE/1935537‑1935577
|
ttAATTTCAAACATGGTCTGATTTCTTTTTTTGAGCTAGTc < 1:1104746/41‑1 (MQ=255)
ttAATTTCAAACATGGTCTGATTTCTTTTTTTGAGCTAGTc < 1:1139056/41‑1 (MQ=255)
ttAATTTCAAACATGGTCTGATTTCTTTTTTTGAGCTAGTc < 1:1231093/41‑1 (MQ=255)
ttAATTTCAAACATGGTCTGATTTCTTTTTTTGAGCTAGTc < 1:162992/41‑1 (MQ=255)
ttAATTTCAAACATGGTCTGATTTCTTTTTTTGAGCTAGTc < 1:1792583/41‑1 (MQ=255)
ttAATTTCAAACATGGTCTGATTTCTTTTTTTGAGCTAGTc < 1:301124/41‑1 (MQ=255)
ttAATTTCAAACATGGTCTGATTTCTTTTTTTGAGCTAGTc < 1:518522/41‑1 (MQ=255)
ttAATTTCAAACATGGTCTGATTTCTTTTTTTGAGCTAGTc < 1:601398/41‑1 (MQ=255)
ttAATTTCAAACATGGTCTGATTTCTTTTTTTGAGCTAGTc < 1:631647/41‑1 (MQ=255)
ttAATTTCAAACATGGTCTGATTTCTTTTTTTGAGCTAGTc < 1:835952/41‑1 (MQ=255)
ttAATTTCAAACATGGTCTGATTTCTTTTTTTGAGCTAGTc < 1:935698/41‑1 (MQ=255)
tAATTTCAAACATGGTCTGATTTCTTTTTTTGAGCTAGTc < 1:575717/40‑1 (MQ=255)
aaTTTCAAACATGGTCTGATTTCTTTTTTTGAGCTAGTc < 1:714845/39‑1 (MQ=255)
|
TTAATTTCAAACATGGTCTGATTTCTTTTATTGAGCTAGTC > minE/1935537‑1935577
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
