Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F28 I2 R2
|
356 |
55.1 |
4139924 |
95.4% |
3949487 |
63.8 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
1,995,272 |
T→C |
S524S (TCA→TCG) |
uvrC ← |
excinuclease UvrABC, endonuclease subunit |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 1,995,272 | 0 | T | C | 98.6%
| 245.5
/ ‑4.2
| 69 | S524S (TCA→TCG) | uvrC | excinuclease UvrABC, endonuclease subunit |
| Reads supporting (aligned to +/- strand): ref base T (0/1); new base C (40/28); total (40/29) |
| Fisher's exact test for biased strand distribution p-value = 4.20e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.58e-01 |
ACGGTGCCCGCCAATCGCGTGATCATGTGATTCATCGCGAATATGCTGGATAACATGCAGCGCGGGTGAATCTGGCGGCAAACTAAATCCCTCACCTTCCGGCTCAAAGAACAGCGTTTCCAGTCCAGCCTTAC > W3110S.gb/1995206‑1995339
|
aCGGTGCCCGCCAATCGCGTGATCATGTGATTCATCGCGAATATGCTGGATAACATGCAGCGCGGGTGaa < 1:3460796/70‑1 (MQ=255)
cccGCCAATCGCGTGATCATGTGATTCATCGCGAATATGCTGGATAACATGCAGCGCGGGCGAATCTggc < 1:1895154/70‑1 (MQ=255)
cccGCCAATCGCGTGATCATGTGATTCATCGCGAATATGCTGGATAACATGCAGCGCGGGCGAATCTggc < 1:2797983/70‑1 (MQ=255)
gTGATCATGTGATTCATCGCGAATATGCTGGATAACATGCAGCGCGGGCGaa > 1:1519316/1‑52 (MQ=255)
gTGATCATGTGATTCATCGCGAATATGCTGGATAACATGCAGCGCGGGCGAATCTGGCGGCAAACTAAATc > 1:2796160/1‑71 (MQ=255)
gTGATCATGTGATTCATCGCGAATATGCTGGATAACATGCAGCGCGGGCGAATCTGGCGGCAAACTAAATc > 1:1396763/1‑71 (MQ=255)
gTGATCATGTGATTCATCGCGAATATGCTGGATAACATGCAGCGCGGGCGAATCTGGCGGCAAACTAAATc > 1:704875/1‑71 (MQ=255)
tgATTCATCGCGAATATGCTGGATAACATGCAGCGCGGGCGAATCTGGCGGCAAACTAAATCCCTCACCtt > 1:1726018/1‑71 (MQ=255)
tgATTCATCGCGAATATGCTGGATAACATGCAGCGCGGGCGAATCTGGCGGCAAACTAAATCCCTCACCtt > 1:3677871/1‑71 (MQ=255)
tgATTCATCGCGAATATGCTGGATAACATGCAGCGCGGGCGAATCTGGCGGCAAACTAAATCCCTCACCtt > 1:3976495/1‑71 (MQ=255)
aTTCATCGCGAATATGCTGGATAACATGCAGCGCGGGCGAATCTGGCGGCAAACTAAATCCCTCACCTTcc > 1:1739506/1‑71 (MQ=255)
aTTCATCGCGAATATGCTGGATAACATGCAGCGCGGGCGAATCTGGCGGCAAACTAAATCCCTCACCTTc > 1:1161169/1‑70 (MQ=255)
gAATATGCTGGATAACATGCAGCGCGGGCGAATCTGGCGGCAAACTAAATc > 1:3395305/1‑51 (MQ=255)
gAATATGCTGGATAACATGCAGCGCGGGCGAATCTGGCGGCAAACTAAATc > 1:1376740/1‑51 (MQ=255)
gAATATGCTGGATAACATGCAGCGCGGGCGAATCTGGCGGCAAACTAAATc > 1:3184129/1‑51 (MQ=255)
gAATATGCTGGATAACATGCAGCGCGGGCGAATCTGGCGGCAAACTAAATCCCTCACCtt > 1:3161071/1‑60 (MQ=255)
gAATATGCTGGATAACATGCAGCGCGGGCGAATCTGGCGGCAAACTAAATCCCTCACCtt > 1:3296626/1‑60 (MQ=255)
gAATATGCTGGATAACATGCAGCGCGGGCGAATCTGGCGGCAAACTAAATCCCTCACCtt > 1:1859358/1‑60 (MQ=255)
gAATATGCTGGATAACATGCAGCGCGGGCGAATCTGGCGGCAAACTAAATCCCTCACCTTCCGGCTCAAAg < 1:2386343/71‑1 (MQ=255)
gAATATGCTGGATAACATGCAGCGCGGGCGAATCTGGCGGCAAACTAAATCCCTCACCTTCCGGCTCAAAg < 1:795391/71‑1 (MQ=255)
gAATATGCTGGATAACATGCAGCGCGGGCGAATCTGGCGGCAAACTAAATCCCTCACCTTCCGGCTCAAAg < 1:2510734/71‑1 (MQ=255)
gAATATGCTGGATAACATGCAGCGCGGGCGAATCTGGCGGCAAACTAAATCCCTCACCTTCCGGCTCAAAg < 1:1364761/71‑1 (MQ=255)
tatGCTGTATAACATGCAGCGCGGGCGAATCTGGCGGCAAACTAAATCCCTCACCTTCCGGCTCAAAg < 1:1824313/68‑1 (MQ=255)
ggATAACATGCAGCGCGGGCGAATCTGGCGGCAAACTAAATCCCTCACCTTCCGGCTCAAAGAACAGCGtt > 1:3162168/1‑71 (MQ=255)
ggATAACATGCAGCGCGGGCGAATCTGGCGGCAAACTAAATCCCTCACCTTCCGGCTCAAAGAACAGCGtt > 1:569247/1‑71 (MQ=255)
gATAACATGCAGCGCGGGCGAATCTGGCGGCAAACTAAATCCCTCACCTTCCGGCTCAAAGAACAGCGtt < 1:617067/70‑1 (MQ=255)
gATAACATGCAGCGCGGGCGAATCTGGCGGCAAACTAAATCCCTCACCTTCCGGCTCAAAGAACAGCGtt < 1:2721017/70‑1 (MQ=255)
gATAACATGCAGCGCGGGCGAATCTGGCGGCAAACTAAATCCCTCACCTTCCGGCTCAAAGAACAGCGtt < 1:2416099/70‑1 (MQ=255)
gATAACATGCAGCGCGGGCGAATCTGGCGGCAAACTAAATCCCTCACCTTCCGGCTCAAAGAACAGCGtt < 1:356494/70‑1 (MQ=255)
gATAACATGCAGCGCGGGCGAATCTGGCGGCAAACTAAATCCCTCACCTTCCGGCTCAAAGAACAGCGtt < 1:3583549/70‑1 (MQ=255)
gATAACATGCAGCGCGGGCGAATCTGGCGGCAAACTAAATCCCTCACCTTCCGGCTCAAAGAACAGCGtt < 1:3620564/70‑1 (MQ=255)
gATAACATGCAGCGCGGGCGAATCTGGCGGCAAACTAAATCCCTCACCTTCCGGCTCAAAGAACAGCGtt < 1:3886652/70‑1 (MQ=255)
gATAACATGCAGCGCGGGCGAATCTGGCGGCAAACTAAATCCCTCACCTTCCGGCTCAAAGAACAGCGtt < 1:904355/70‑1 (MQ=255)
gATAACATGCAGCGCGGGCGAATCTGGCGGCAAACTAAATCCCTCACCTTCCGGCTCAAAGAACAGCGtt < 1:3839117/70‑1 (MQ=255)
tAACATGCAGCGCGGGCGAATCTGGCGGCAAACTAAATCCCTCACCTTCCGGCTCAAAGAACAGCGTTtc > 1:922428/1‑70 (MQ=255)
tAACATGCAGCGCGGGCGAATCTGGCGGCAAACTAAATCCCTCACCTTCCGGCTCAAAGAACAGCGTTtc > 1:2581769/1‑70 (MQ=255)
tAACATGCAGCGCGGGCGAATCTGGCGGCAAACTAAATCCCTCACCTTCCGGCTCAAAGAACAGCGTTtc > 1:845316/1‑70 (MQ=255)
tAACATGCAGCGCGGGCGAATCTGGCGGCAAACTAAATCCCTCACCTTCCGGCTCAAAGAACAGCGTTtc > 1:2255935/1‑70 (MQ=255)
aCATGCAGCGCGGGCGAATCTGGCGGCAAACTAAATCCCTCACCTTCCGGCTCAAAGAACAGCGttta > 1:1976630/1‑67 (MQ=255)
aCATGCAGCGCGGGCGAATCTGGCGGCAAACTAAATCCCTCACCTTCCGGCTCAAAGAACAGCGTTtc > 1:2480811/1‑68 (MQ=255)
gCAGCGCGGGCGAATCTGGCGGCAAACTAAATCCCTCACCTTCCGGCTCAAAGAACAGCGtt > 1:4082800/1‑62 (MQ=255)
gCAGCGCGGGCGAATCTGGCGGCAAACTAAATCCCTCACCTTCCGGCTCAAAGAACAGCGtt > 1:3062720/1‑62 (MQ=255)
gCAGCGCGGGCGAATCTGGCGGCAAACTAAATCCCTCACCTTCCGGCTCAAAGAACAGCGTTtccagtc > 1:3148894/1‑69 (MQ=255)
gCAGCGCGGGCGAATCTGGCGGCAAACTAAATCCCTCACCTTCCGGCTCAAAGAACAGCGTTtccagtc > 1:2348827/1‑69 (MQ=255)
gCAGCGCGGGCGAATCTGGCGGCAAACTAAATCCCTCACCTTCCGGCTCAAAGAACAGCGTTtccagtc > 1:2076821/1‑69 (MQ=255)
gCAGCGCGGGCGAATCTGGCGGCAAACTAAATCCCTCACCTTCCGGCTCAAAGAACAGCGTTtccagtc > 1:1722489/1‑69 (MQ=255)
gCAGCGAGGGCGAATCTGGCGGCAAACTAAATCCCTCACCTTCCGGCTCAAAGAACAGCGTTtccagtc > 1:5621/1‑69 (MQ=255)
cAGCGCGGGCGAATCTGGCGGCAAACTAAATCCCTCACCTTCCGGCTCAAAGAACAGCGTTtcca > 1:2248382/1‑65 (MQ=255)
cAGCGCGGGCGAATCTGGCGGCAAACTAAATCCCTCACCTTCCGGCTCAAAGAACAGCGTTtcca > 1:1709822/1‑65 (MQ=255)
cAGCGCGGGCGAATCTGGCGGCAAACTAAATCCCTCACCTTCCGGCTCAAAGAACAGCGTTtcca > 1:231827/1‑65 (MQ=255)
cAGCGCGGGCGAATCTGGCGGCAAACTAAATCCCTCACCTTCCGGCTCAAAGAACAGCGTTtcca > 1:2864977/1‑65 (MQ=255)
aGCGCGGGCGAATCTGGCGGCAAACTAAATCCCTCACCTTCCGGCTCAAAGAACAGCGTTTCCAGTCCAGc < 1:1558867/71‑1 (MQ=255)
gcgGGCTAATCTGGCGGCAAACTAAATCCCTCACCTTCCGGCTAAAAGAACAGCGTTTCCAGTCCAGCCtt < 1:3802766/71‑1 (MQ=255)
gcgGGCGAATCTGGCGGCAAACTAAATCCCTCACCTTCCGGCTCAAAGAACAGCGTTTCCAGTCCAGCCtt < 1:880130/71‑1 (MQ=255)
gcgGGCGAATCTGGCGGCAAACTAAATCCCTCACCTTCCGGCTCAAAGAACAGCGTTTCCAGTCCAGCCtt < 1:1382140/71‑1 (MQ=255)
gcgGGCGAATCTGGCGGCAAACTAAATCCCTCACCTTCCGGCTAAAAGAACAGCGTTTCCAGTCCAGCCtt < 1:1058528/71‑1 (MQ=255)
gcgGGCGAATCTGGCGGCAAACTAAATCCCTCACCTTCCGGCTAAAAGAACAGCGTTTCCAGTCCAGCCtt < 1:3733678/71‑1 (MQ=255)
gcgGGCGAATCTGGCGGCAAACTAAATCCCTCACCTTCCGGCTAAAAGAACAGCGTTTCCAGTCCAGCCtt < 1:2792076/71‑1 (MQ=255)
gcgGGCGAATCTGGCGGCAAACTAAATCCCTCACCTTCCGGCTAAAAGAACAGCGTTTCCAGTCCAGCCtt < 1:2531389/71‑1 (MQ=255)
gcgGGCGAATCTGGCGGCAAACTAAATCCCTCACCTTCCGGCTAAAAGAACAGCGTTTCCAGTCCAGCCtt < 1:1457946/71‑1 (MQ=255)
cgGGCGAATCTGGCGGCAAACTAAATCCCTCACCtt > 1:2686891/1‑36 (MQ=255)
cgGGCGAATCTGGCGGCAAACTAAATCCCTCACCTTCCGGCTCAAAGAACAGCGTTtccagtc > 1:32317/1‑63 (MQ=255)
cgGGCGAATCTGGCGGCAAACTAAATCCCTCACCTTCCGGCTCAAAGAACAGCGTTtccagtc > 1:2601467/1‑63 (MQ=255)
cgGGCGAATCTGGCGGCAAACTAAATCCCTCACCTTCCGGCTCAAAGAACAGCGTTtccagtc > 1:2459122/1‑63 (MQ=255)
cgGGCGAATCTGGCGGCAAACTAAATCCCTCACCTTCCGGCTCAAAGAACAGCGTTtccagtc > 1:988207/1‑63 (MQ=255)
cgGGCGAATCTGGCGGCAAACTAAATCCCTCACCTTCCGGCTCAAAGAACAGCGTTtccagt > 1:1263049/1‑62 (MQ=255)
cgGGCGAATCTGGCGGCAAACTAAATCCCTCACCTTCCGGCTCAAAGAACAGCGTTTCCAGTCCAGCCtt < 1:2812295/70‑1 (MQ=255)
cgGGCGAATCTGGCGGCAAACTAAATCCCTCACCTTCCGGCTCAAAGAACAGCGTTTCCAGTCCAGCCtt < 1:1014484/70‑1 (MQ=255)
gggCGAATCTGGCGGCAAACTAAATCCCTCACCTTCCGGCTCAAAGAACAGCGTTTCCAGTCCAGCCTTAc < 1:1655103/71‑1 (MQ=255)
|
ACGGTGCCCGCCAATCGCGTGATCATGTGATTCATCGCGAATATGCTGGATAACATGCAGCGCGGGTGAATCTGGCGGCAAACTAAATCCCTCACCTTCCGGCTCAAAGAACAGCGTTTCCAGTCCAGCCTTAC > W3110S.gb/1995206‑1995339
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
