Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F28 I2 R2
|
356 |
55.1 |
4139924 |
95.4% |
3949487 |
63.8 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
2,077,980 |
C→T |
Y355Y (TAC→TAT) |
yeeR → |
predicted membrane protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 2,077,980 | 0 | C | T | 90.6%
| 185.1
/ 8.1
| 64 | Y355Y (TAC→TAT) | yeeR | predicted membrane protein |
| Reads supporting (aligned to +/- strand): ref base C (0/6); new base T (36/22); total (36/28) |
| Fisher's exact test for biased strand distribution p-value = 5.02e-03 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
CCTCCTGCGGGCTGACAGAAGTACATCATGCCCCGGAAAATCATAAGCCGGATCCGGAATGGTACTGCTCCTCTCTTTGTCGCGAAACAGAAACACTGTGTCAGGAAATTTATGAACGCCCTTAC > W3110S.gb/2077916‑2078040
|
cctcctGCGGGCTGACAGAAGTACATCATGCCCCGGAAAATCATAAGCCGGATCCGGAATGGTATTGctcc > 1:2638885/1‑71 (MQ=255)
cctcctGCGGGCTGACAGAAGTACATCATGCCCCGGAAAATCATAAGCCGGATCCGGAATGGTATTGctcc > 1:2232903/1‑71 (MQ=255)
cAGAAGTACATCATGCCCCGGAAAATCATAAGCCGGATCCGGAATGGTATTGCTCCTCTCTTTGTCGCga < 1:3686316/70‑1 (MQ=255)
cAGAAGTACATCATGCCCCGGAAAATCATAAGCCGGATCCGGAATGGTATTGCTCCTCTCTTTGTCGCga < 1:3100583/70‑1 (MQ=255)
catcatGCCCCGGAAAATCATAAGCCGGATCCGGAATGGTATTGCTCCTCTCTTTGTCGCgaaacagaaa > 1:3558037/1‑70 (MQ=255)
catcatGCCCCGGAAAATCATAAGCCGGATCCGGAATGGTATTGCTCCTCTCTTTGTCGCgaaacagaaa > 1:2391294/1‑70 (MQ=255)
catcatGCCCCGGAAAATCATAAGCCGGATCCGGAATGGTATTGCTCCTCTCTTTGTCGCgaaacagaaa < 1:450681/70‑1 (MQ=255)
catcatGCCCCGGAAAATCATAAGCCGGATCCGGAATGGTATTGCTCCTCTCTTTGTCGCgaaacagaaa > 1:2327978/1‑70 (MQ=255)
catcatGCCCCGGAAAATCATAAGCCGGATCCGGAATGGTATTGCTCCTCTCTTTGTCGCgaaacagaaa < 1:4024492/70‑1 (MQ=255)
catcatGCCCCGGAAAATCATAAGCCGGATCCGGAATGGTATTGCTCCTCTCTTTGTCGCgaaacagaaa < 1:1732856/70‑1 (MQ=255)
catcatGCCCCGGAAAATCATAAGCCGGATCCGGAATGGTATTGCTCCTCTCTTTGTCGCgaaacagaaa < 1:36023/70‑1 (MQ=255)
catcatGCCCCGGAAAATCATAAGCCGGATCCGGAATGGTATTGCTCCTCTCTTTGTCGCgaaacagaaa > 1:1589124/1‑70 (MQ=255)
catcatGCCCCGGAAAATCATAAGCCGGATCCGGAATGGTATTGCTCCTCTCTTTGTCGCgaaacagaaa < 1:3131122/70‑1 (MQ=255)
catcatGCCCCGGAAAATCATAAGCCGGATCCGGAATGGTATTGCTCCTCTCTTTGTCGCgaaacagaaa < 1:1360933/70‑1 (MQ=255)
catcatGCCCCGGAAAATCATAAGCCGGATCCGGAATGGTATTGCTCCTCTCTTTGTCGCgaaacagaaa < 1:3135855/70‑1 (MQ=255)
catcatGCCCCGGAAAATCATAAGCCGGATCCGGAATGGTATTGCTCCTCTCTTTGTCGCgaaacagaaa > 1:3559169/1‑70 (MQ=255)
tcatGCCCCGGAAAATCATAAGCCGGATCCGGAATGGTATTGCTCCTCTCTTTGTcg > 1:1894500/1‑57 (MQ=255)
tcatGCCCCGGAAAATCATAAGCCGGATCCGGAATGGTATTGCTCCTCTCTTTGTcg > 1:162248/1‑57 (MQ=255)
tcatGCCCCGGAAAATCATAAGCCGGATCCGGAATGGTATTGCTCCTCTCTTTGTcg > 1:2002752/1‑57 (MQ=255)
tcatGCCCCGGAAAATCATAAGCCGGATCCGGAATGGTATTGCTCCTCTCTTTGTcg > 1:428017/1‑57 (MQ=255)
tcatGCCCCGGAAAATCATAAGCCGGATCCGGAATGGTATTGCTCCTCTCTTTGTcg > 1:1380273/1‑57 (MQ=255)
tcatGCCCCGGAAAATCATAAGCCGGATCCGGAATGGTATTGCTCCTCTCTTTGTc > 1:2956861/1‑56 (MQ=255)
tcatGCCCCGGAAAATCATAAGCCGGATCCGGAATGGTATTGCTCCTCTCTTTGTCGCGAAACAGAAacac < 1:3459303/71‑1 (MQ=255)
tcatGCCCCGGAAAATCATAAGCCGGATCCGGAATGGTATTGCTCCTCTCTTTGTCGCGAAACAGAAacac < 1:567577/71‑1 (MQ=255)
tcatGCCCCGGAAAATCATAAGCCGGATCCGGAATGGTATTGCTACTCTCTTTGTcg > 1:2490892/1‑57 (MQ=255)
tcatGCCCCGGAAAATCATAAG‑‑GGATCCGGAATGGTATTGCTCCTCTCTTTGTcg > 1:2663035/1‑55 (MQ=255)
cgccGGAAAATCATAAGCCGGATCCGGAATGGTATTGCTCCTCTCTTTGTCGCGAAACAGAAACACTgtgt > 1:883182/3‑71 (MQ=255)
ccccGGAAAATCATAAGCCGGATCCGGAATGGTATTGCTCCTCTCTTTGTCGCGAAACAGAAACACtgtg > 1:851855/1‑70 (MQ=255)
ccccGGAAAATCATAAGCCGGATCCGGAATGGTATTGCTCCTCTCTTTGTCGCGAAACAGAAACACtgtg > 1:1297373/1‑70 (MQ=255)
ccccGGAAAATCATAAGCCGGATCCGGAATGGTATTGCTCCTCTCTTTGTCGCGAAACAGAAACACtgtg > 1:1260480/1‑70 (MQ=255)
ccccGGAAAATCATAAGCCGGATCCGGAATGGTATTGCTCCTCTCTTTGTCGCGAAACAGAAACACTgtgt > 1:1999962/1‑71 (MQ=255)
ccccGGAAAATCATAAGCCGGATCCGGAATGGTATTGCTCCTCTCTTTGTCGCGAAACAGAAACACTgtgt > 1:3712006/1‑71 (MQ=255)
ccccGGAAAATCATAAGCCGGATCCGGAATGGTATTGCTCCTCTCTTTGTCGCGAAACAGAAACACTgtgt > 1:1325611/1‑71 (MQ=255)
ccccGGAAAATCATAAGCCGGATCCGGAATGGTATTGCTCCTCTCTTTGTCGCGAAACAGAAACACTgtgt > 1:314778/1‑71 (MQ=255)
aaTCATAAGCCGGATCCGGAATGGTATTGCTCCTCTCTTTGTCGCga < 1:3823589/47‑1 (MQ=255)
aaTCATAAGCCGGATCCGGAATGGTATTGCTCCTCTCTTTGTCGCga < 1:647175/47‑1 (MQ=255)
aaTCATAAGCCGGATCCGGAATGGTATTGCTCCTCTCTTTGTCGCga < 1:709581/47‑1 (MQ=255)
aaTCATAAGCCGGATCCGGAATGGTATTGCTCCTCTCTTTGTCGCga < 1:816056/47‑1 (MQ=255)
aaTCATAAGCCGGATCCGGAATGGTATTGCTCCTCTCTTTGTCGCga < 1:3125874/47‑1 (MQ=255)
aaTCATAAGCCGGATCCGGAATGGTATTGCTCCTCTCTTTGTCGCga < 1:3314045/47‑1 (MQ=255)
aaTCATAAGCCGGATCCGGAATGGTATTGCTCCTCTCTTTGTCGCga < 1:2950994/47‑1 (MQ=255)
aaTCATAAGCCGGATCCGGAATGGTATTGCTCCTCTCTTTGTCGCga < 1:1945499/47‑1 (MQ=255)
aTCCGGAATGGTATTGCTCCTCTCTTTGTCGCGAAACAGAAACACTGTGTCAGGAAATTTATGAACGCCCt < 1:1011573/71‑1 (MQ=255)
aTCCGGAATGGTATTGCTCCTCTCTTTGTCGCGAAACAGAAACACTGTGTCAGGAAATTTATGAACGCCCt < 1:3939035/71‑1 (MQ=255)
aTCCGGAATGGTATTGCTCCTCTCTTTGTCGCGAAACAGAAACACTGTGTCAGGAAATTTATGAACGCCCt < 1:2077821/71‑1 (MQ=255)
tCCGGAATGGTATTGCTCCTCTCTTTGTCGCGAAACAGAAACACTGTGTCAGGAAATTTATGa > 1:163348/1‑63 (MQ=255)
tCCGGAATGGTATTGCTCCTCTCTTTGTCGCGAAACAGAAACACTGTGTCAGGAAATTTATGAACGCCCt > 1:3430607/1‑70 (MQ=255)
tCCGGAATGGTATTGCTCCTCTCTTTGTCGCGAAACAGAAACACTGTGTCAGGAAATTTATGAACGCCCt > 1:3794979/1‑70 (MQ=255)
tCCGGAATGGTATTGCTCCTCTCTTTGTCGCGAAACAGAAACACTGTGTCAGGAAATTTATGAACGCCCt > 1:2806011/1‑70 (MQ=255)
tCCGGAATGGTATTGCTCCTCTCTTTGTCGCGAAACAGAAACACTGTGTCAGGAAATTTATGAACGCCCt > 1:696447/1‑70 (MQ=255)
tCCGGAATGGTATTGCTCCTCTCTTTGTCGCGAAACAGAAACACTGTGTCAGGAAATTTATGAACGCCCt > 1:2386811/1‑70 (MQ=255)
ggAATGGTATTGCTCCTCTCTTTGTCGCgaaacag > 1:309151/1‑35 (MQ=255)
ggAATGGTATTGCTCCTCTCTTTGTCGCGAAACAGAAACACTGTGTCAGGAAATTTATGAACGCCCTTac > 1:2701629/1‑70 (MQ=255)
ggAATGGTATTGCTCCTCTCTTTGTCGCGAAACAGAAACACTGTGTCAGGAAATTTATGAACGCCCTTac > 1:1499525/1‑70 (MQ=255)
ggAATGGTATTGCTCCTCTCTTTGTCGCGAAACAGAAACACTGTGTCAGGAAATTTATGAACGCCCTTac > 1:464125/1‑70 (MQ=255)
ggAATGGTATTGCTCCTCTCTTTGTCGCGAAACAGAAACACTGTGTCAGGAAATTTATGAACGCCCTTac > 1:412991/1‑70 (MQ=255)
ggAATGGTATTGCTCCTCTCTTTGTCGCGAAACAGAAACACTGTGTCAGGAAATTTATGAACGCCCTTac > 1:3896334/1‑70 (MQ=255)
ggAATGGTATTGCTCCTCTCTTTGTCGCGAAACAGAAACACTGTGTCAGGAAATTTATGAACGCCCTTac > 1:3664147/1‑70 (MQ=255)
tGGTACTGCTCCTCTCTTTGTCGCGAAACAGAAACACTGTGTCAGGaaagt < 1:1971043/51‑3 (MQ=255)
tGGTACTGCTCCTCTCTTTGTCGCGAAACAGAAACACTGTGTCAGGAAAtt < 1:2645054/51‑1 (MQ=255)
tGGTACTGCTCCTCTCTTTGTCGCGAAACAGAAACACTGTGTCAGGAAAtt < 1:3448319/51‑1 (MQ=255)
tGGTACTGCTCCTCTCTTTGTCGCGAAACAGAAACACTGTGTCAGGAAAtt < 1:1951754/51‑1 (MQ=255)
tGGTACTGCTCCTCTCTTTGTCGCGAAACAGAAACACTGTGTCAGGAAAtt < 1:3393240/51‑1 (MQ=255)
ggTACTGCTCCTCTCTTTGTCGCGAAACAGAAACACTGTGTCAGGAAAtt < 1:900873/50‑1 (MQ=255)
|
CCTCCTGCGGGCTGACAGAAGTACATCATGCCCCGGAAAATCATAAGCCGGATCCGGAATGGTACTGCTCCTCTCTTTGTCGCGAAACAGAAACACTGTGTCAGGAAATTTATGAACGCCCTTAC > W3110S.gb/2077916‑2078040
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
