Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F28 I2 R2
|
356 |
55.1 |
4139924 |
95.4% |
3949487 |
63.8 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
2,258,347 |
C→T |
E58E (GAG→GAA) |
rihB ← |
ribonucleoside hydrolase 2 |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 2,258,347 | 0 | C | T | 100.0%
| 131.1
/ NA
| 42 | E58E (GAG→GAA) | rihB | ribonucleoside hydrolase 2 |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (26/16); total (26/16) |
GGCAACGATTTGTTGACGCATAATGGGCTGCGGCATCCCCGCATAAACCGGAACATTAATCTCCAGTTTCTGGCAAACATTCAGGCCATTAATTAATGTTTTATCAAGCGTCTGATTAC > W3110S.gb/2258287‑2258405
|
ggCAACGATTTGTTGACGCATAATGGGCTGCGGCATCCCCGCATAAACCGGAACATTAATTTCCAGTTTCt < 1:941123/71‑1 (MQ=255)
ggCAACGATTTGTTGACGCATAATGGGCTGCGGCATCCCCGCATAAACCGGAACATTAATTTCCAGTTTCt < 1:3137175/71‑1 (MQ=255)
gATTTGTTGACGCATAATGGGCTGCGGCATCCCCGCATAAACCGGAACATTAATTTCCAGTTTCTGGCaaa > 1:562668/1‑71 (MQ=255)
gATTTGTTGACGCATAATGGGCTGCGGCATCCCCGCATAAACCGGAACATTAATTTCCAGTTTCTGGCaaa > 1:2571194/1‑71 (MQ=255)
gATTTGTTGACGCATAATGGGCTGCGGCATCCCCGCATAAACCGGAACATTAATTTCCAGTTTCTGGCaaa > 1:1852782/1‑71 (MQ=255)
gATTTGTTGACGCATAATGGGCTGCGGCATCCCCGCATAAACCGGAACATTAATTTCCAGTTTCTGGCaaa > 1:3742798/1‑71 (MQ=255)
gATTTGTTGACGCATAATGGGCTGCGGCATCCCCGCATAAACCGGAACATTAATTTCCAGTTTCTGGCaaa > 1:3737979/1‑71 (MQ=255)
gATTTGTTGACGCATAATGGGCTGCGGCATCCCCGCATAAACCGGAACATTAATTTCCAGTTTCTGGCaaa > 1:3185774/1‑71 (MQ=255)
gACGCATAATGGGCTGCGGCATCCCCGCATAAACCGGAACATTAATTTCCAGTTTCTGGCAAACATTCAgg > 1:1246461/1‑71 (MQ=255)
gACGCATAATGGGCTGCGGCATCCCCGCATAAACCGGAACATTAATTTCCAGTTTCTGGCAAACATTCAgg > 1:1436108/1‑71 (MQ=255)
gACGCATAATGGGCTGCGGCATCCCCGCATAAACCGGAACATTAATTTCCAGTTTCTGGCAAACATTCAgg > 1:2349398/1‑71 (MQ=255)
gACGCATAATGGGCTGCGGCATCCCCGCATAAACCGGAACATTAATTTCCAGTTTCTGGCAAACATTCAg < 1:3079906/70‑1 (MQ=255)
tCCCCGCATAAACCGGAACATTAATTTCCAGTTTCTGGCAAACATTCAg < 1:292610/49‑1 (MQ=255)
tCCCCGCATAAACCGGAACATTAATTTCCAGTTTCTGGCAAACATTCAg < 1:3291283/49‑1 (MQ=255)
tCCCCGCATAAACCGGAACATTAATTTCCAGTTTCTGGCAAACATTCAg < 1:1980619/49‑1 (MQ=255)
tCCCCGCATAAACCGGAACATTAATTTCCAGTTTCTGGCAAACATTCAg < 1:344159/49‑1 (MQ=255)
tCCCCGCATAAACCGGAACATTAATTTCCAGTTTCTGGCAAACATTCAGGCCa > 1:4134166/1‑53 (MQ=255)
tCCCCGCATAAACCGGAACATTAATTTCCAGTTTCTGGCAAACATTCAGGCCa > 1:2961817/1‑53 (MQ=255)
tCCCCGCATAAACCGGAACATTAATTTCCAGTTTCTGGCAAACATTCAGGCCa > 1:1475226/1‑53 (MQ=255)
tCCCCGCATAAACCGGAACATTAATTTCCAGTTTCTGGCAAACATTCAGGCCa > 1:1842083/1‑53 (MQ=255)
tCCCCGCATAAACCGGAACATTAATTTCCAGTTTCTGGCAAACATTCAGGCCa > 1:1935809/1‑53 (MQ=255)
tCCCCGCATAAACCGGAACATTAATTTCCAGTTTCTGGCAAACATTCAGGCCa > 1:19861/1‑53 (MQ=255)
tCCCCGCATAAACCGGAACATTAATTTCCAGTTTCTGGCAAACATTCAGGCCa > 1:1962909/1‑53 (MQ=255)
cGCATAAACCGGAACATTAATTTCCAGTTTCTGTCAAACATTCAGGCCATTAATTAATGTTTTATCAAGCg < 1:2020097/71‑1 (MQ=255)
cGCATAAACCGGAACATTAATTTCCAGTTTCTGGCaaa > 1:3580221/1‑38 (MQ=255)
cGCATAAACCGGAACATTAATTTCCAGTTTCTGGCAAACATTCAGGCCATTAATTAATGTTTTATCAAGCg < 1:2624398/71‑1 (MQ=255)
cctgAACATTAATTTCCAGTTTCTGGCAAACATTCAGGCCATTAATTAATGTTTTATCAAGCGTCTGATTa < 1:2290823/68‑1 (MQ=255)
ccGGAACATTAATTTCCAGTTTCTGGCAAACATTCAGGCCATTAATTAATGTTTTATCAAGCGTCTGATTa < 1:69315/71‑1 (MQ=255)
ccGGAACATTAATTTCCAGTTTCTGGCAAACATTCAGGCCATTAATTAATGTTTTATCAAGCGTCTGATTa < 1:3642112/71‑1 (MQ=255)
ccGGAACATTAATTTCCAGTTTCTGGCAAACATTCAGGCCATTAATTAATGTTTTATCAAGCGTCTGATTa < 1:2625748/71‑1 (MQ=255)
ccGGAACATTAATTTCCAGTTTCTGGCAAACATTCAGGCCATTAATTAATGTTTTATCAAGCGTCTGATTa < 1:3237399/71‑1 (MQ=255)
ccGGAACATTAATTTCCAGTTTCTGGCAAACATTCAGGCCATTAATTAATGTTTTATCAAGCGTCTGATTa < 1:3131434/71‑1 (MQ=255)
ccGGAACATTAATTTCCAGTTTCTGGCAAACATTCAGGCCATTAATTAATGTTTTATCAAGCGTCTGATTa < 1:296448/71‑1 (MQ=255)
cGGAACATTAATTTCCAGTTTCTGGCAAACATTCAGGCCATTAATTAATg > 1:927065/1‑50 (MQ=255)
cGGAACATTAATTTCCAGTTTCTGGCAAACATTCAGGCCATTAATTAATGt > 1:1914584/1‑51 (MQ=255)
cGGAACATTAATTTCCAGTTTCTGGCAAACATTCAGGCCATTAATTAATGTTTTATCAAGCGTCTGATTAc > 1:866858/1‑71 (MQ=255)
cGGAACATTAATTTCCAGTTTCTGGCAAACATTCAGGCCATTAATTAATGTTTTATCAAGCGTCTGATTAc > 1:1237620/1‑71 (MQ=255)
cGGAACATTAATTTCCAGTTTCTGGCAAACATTCAGGCCATTAATTAATGTTTTATCAAGCGTCTGATTAc > 1:3563960/1‑71 (MQ=255)
cGGAACATTAATTTCCAGTTTCTGGCAAACATTCAGGCCATTAATTAATGTTTTATCAAGCGTCTGATTAc > 1:852641/1‑71 (MQ=255)
cGGAACATTAATTTCCAGTTTCTGGCAAACATTCAGGCCATTAATTAATGTTTTATCAAGCGTCTGATTAc > 1:2421159/1‑71 (MQ=255)
cGGAACATTAATTTCCAGTTTCTGGCAAACATTCAGGCCATTAATTAATGTTTTATCAAGCGTCTGATTAc > 1:1089178/1‑71 (MQ=255)
cGGAACATTAATTTCCAGTTTCTGGCAAACATTCAGGCCATTAATTAATGTTTTATCAAGCGTCTGATTAc > 1:2826306/1‑71 (MQ=255)
|
GGCAACGATTTGTTGACGCATAATGGGCTGCGGCATCCCCGCATAAACCGGAACATTAATCTCCAGTTTCTGGCAAACATTCAGGCCATTAATTAATGTTTTATCAAGCGTCTGATTAC > W3110S.gb/2258287‑2258405
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
