Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F28 I2 R2
|
356 |
55.1 |
4139924 |
95.4% |
3949487 |
63.8 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
3,447,843 |
A→G |
V1163V (GTT→GTC) |
rpoC ← |
RNA polymerase, beta prime subunit |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 3,447,843 | 0 | A | G | 100.0%
| 226.1
/ NA
| 62 | V1163V (GTT→GTC) | rpoC | RNA polymerase, beta prime subunit |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (28/34); total (28/34) |
ACGGATCGCTACCGTCTACCGGGGTGATAACCAGACGACGTTTACCTTTGGTTTCTTTACCGAAGGAAACGATACCGCTGATTTCAGCCAGGATTGCCGGCTCTTTCGGACGACGTGCTTCGAACAGG > W3110S.gb/3447776‑3447903
|
aCGGATCGCTACCGTCTACCGGGGTGATAACCAGACGACGTTTACCTTTGGTTTCTTTACCGAAGGAGAc > 1:3489204/1‑70 (MQ=255)
aCGGATCGCTACCGTCTACCGGGGTGATAACCAGACGACGTTTACCTTTGGTTTCTTTACCGAAGGAGAc > 1:1417126/1‑70 (MQ=255)
aCGGATCGCTACCGTCTACCGGGGTGATAACCAGACGACGTTTACCTTTGGTTTCTTTACCGAAGGAGAc > 1:2748077/1‑70 (MQ=255)
aCGGATCGCTACCGTCTACCGGGGTGATAACCAGACGACGTTTACCTTTGGTTTCTTTACCGAAGGAGAc > 1:1639302/1‑70 (MQ=255)
aCGGATCGCTACCGTCTACCGGGGTGATAACCAGACGACGTTTACCTTTGGTTTCTTTACCGAAGGAGAc > 1:3265362/1‑70 (MQ=255)
aCGGATCGCTACCGTCTACCGGGGTGATAACCAGACGACGTTTACCTTTGGTTTCTTTACCGAAGGAGAc > 1:624789/1‑70 (MQ=255)
aCGGATCGCTACCGTCTACCGGGGTGATAACCAGACGACGTTTACCTTTGGTTTCTTTACCGAAGGAGAc > 1:3434406/1‑70 (MQ=255)
gATCGCTACCGTCTACCGGGGTGATAACCAGACGACGTTTACCTTTGGTTTCTTTACCGAAGGAGACGATa > 1:1965081/1‑71 (MQ=255)
ggTGATAACCAGACGACGTTTACCTTTGGTTTCTTTACCGAAGGAGACGATACCGCTGATTTCAGCCAgta < 1:46713/71‑3 (MQ=255)
tAACCAGACGACGTTTGCCTTTGGTTTCTTTACCGAAGGAGACGATACCGCTGATTTCAGCCAgg < 1:943004/65‑1 (MQ=255)
tAACCAGACGACGTTTACCTTTGGTTTCTTTACCGAAGGAGACGATACCGCTGATTTCAGCCAgg < 1:2370794/65‑1 (MQ=255)
tAACCAGACGACGTTTACCTTTGGTTTCTTTACCGAAGGAGACGATACCGCTGATTTCAGCCAgg < 1:2412602/65‑1 (MQ=255)
tAACCAGACGACGTTTACCTTTGGTTTCTTTACCGAAGGAGACGATACCGCTGATTTCAGCCAgg < 1:2354908/65‑1 (MQ=255)
tAACCAGACGACGTTTACCTTTGGTTTCTTTACCGAAGGAGACGATACCGCTGATTTCAGCCAgg < 1:1958780/65‑1 (MQ=255)
tAACCAGACGACGTTTACCTTTGGTTTCTTTACCGAAGGAGACGATACCGCTGATTTCAGCCAgg < 1:679121/65‑1 (MQ=255)
tAACCAGACGACGTTTACCTTTGGTTTCTTTACCGAAGGAGACGATACCGCTGATTTCAGCCAgg < 1:1579534/65‑1 (MQ=255)
tAACCAGACGACGTTTACCTTTGGTTTCTTTACCGAAGGAGACGATACCGCTGATTTCAGCCAgg < 1:3317027/65‑1 (MQ=255)
tAACCAGACGACGTTTACCTTTGGTTTCTTTACCGAAGGAGACGATACCGCTGATTTCAGCCAgg < 1:825212/65‑1 (MQ=255)
aaCCAGACGACGTTTACGTTTGGTTTCTTTACCGAAGGAGACGATACCGCTGATTTCAGCCAGGATTGCCg > 1:1047635/1‑71 (MQ=255)
aaCCAGACGACGTTTACCTTTGGTTTCTTTACCGAAGGAGCCGATACCGCTGATTTCAGCCAGGATTGcc > 1:3535840/1‑70 (MQ=255)
aaCCAGACGACGTTTACCTTTGGTTTCTTTACCGAAGGAGACGATACCGCTGATTTCAGCCAGGATTGCCg > 1:313286/1‑71 (MQ=255)
aaCCAGACGACGTTTACCTTTGGTTTCTTTACCGAAGGAGACGATACCGCTGATTTCAGCCAGGATTGCCg > 1:3406580/1‑71 (MQ=255)
aaCCAGACGACGTTTACCTTTGGTTTCTTTACCGAAGGAGACGATACCGCTGATTTCAGCCAGGATTGCCg > 1:2661528/1‑71 (MQ=255)
aaCCAGACGACGTTTACCTTTGGTTTCTTTACCGAAGGAGACGATACCGCTGATTTCAGCCAGGATTGCCg > 1:3330108/1‑71 (MQ=255)
aaCCAGACGACGTTTACCTTTGGTTTCTTTACCGAAGGAGACGATACCGCTGATTTCAGCCAGGATTGCCg > 1:3400969/1‑71 (MQ=255)
aaCCAGACGACGTTTACCTTTGGTTTCTTTACCGAAGGAGACGATACCGCTGATTTCAGCCAGGATTGCCg > 1:2085234/1‑71 (MQ=255)
aaCCAGACGACGTTTACCTTTGGTTTCTTTACCGAAGGAGACGATACCGCTGATTTCAGCCAGGATTGCCg > 1:560064/1‑71 (MQ=255)
aaCCAGACGACGTTTACCTTTGGTTTCTTTACCGAAGGAGACGATACCGCTGATTTCAGCCAGGATTGCCg > 1:751329/1‑71 (MQ=255)
acgacgTTTACCTTTGGTTTCTTTACCGAAGGAGACGATACCGCTGATTTCAGCCAGGATTGCCGGCTCtt < 1:3593652/71‑1 (MQ=255)
acgacgTTTACCTTTGGTTTCTTTACCGAAGGAGACGATACCGCTGATTTCAGCCAGGATTGCCGGCTCtt < 1:3889280/71‑1 (MQ=255)
acgacgTTTACCTTTGGTTTCTTTACCGAAGGAGACGATACCGCTGATTTCAGCCAGGATTGCCGGCTCtt < 1:2545225/71‑1 (MQ=255)
acgacgTTTACCTTTGGTTTCTTTACCGAAGGAGACGATACCGCTGATTTCAGCCAGGATTGCCGGCTCtt < 1:2252493/71‑1 (MQ=255)
acgacgTTTACCTTTGGTTTCTTTACCGAAGGAGACGATACCGCTGATTTCAGCCAGGATTGCCGGCTCtt < 1:1195489/71‑1 (MQ=255)
acgacgTTTACCTTTGGTTTCTTTACCGAAGGAGACGATACCGCTGATTTCAGCCAGGATTGCCGGCTCtt < 1:617147/71‑1 (MQ=255)
acgacgTTTACCTTTGGTTTCTTTACCGAAGGAGACGATACCGCTGATTTCAGCCAGGATTGCCGGCTCtt < 1:3217189/71‑1 (MQ=255)
acgacgTTTACCTTTGGTTTCTTTACCGAAGGAGACGATACCGCTGATTTCAGCCAGGATTGCCGGCTCtt < 1:1658917/71‑1 (MQ=255)
acgacgTTTACCTTTGGTTTCTTTACCGAAGGAGACGATACCGCTGATTTCAGCCAGGATTGCCGGCTCtt < 1:1411745/71‑1 (MQ=255)
acgTTTAGCTTTGGTTTCTTTAGCGAAGGAGACGATACCGCTGATTTCAGCCAgg < 1:4071012/55‑1 (MQ=255)
tACCTTTGGTTTCTTTACCGAAGGAGACGATACCGCTGATTTCAGCCAGGATTGCCGGCTCTTTCGgacga > 1:2375433/1‑71 (MQ=255)
tttGGTTTCTTTACCGAAGGAGACGATACCGCTGATTTCAGCCAGGa > 1:2078199/1‑47 (MQ=255)
tGGTTTCTTTACCGAAGGAGACGATACCGCTGATTTCAGCCAg > 1:3480970/1‑43 (MQ=255)
tGGTTTCTTTACCGAAGGAGACGATACCGCTGATTTCAGCCAg > 1:946530/1‑43 (MQ=255)
tGGTTTCTTTACCGAAGGAGACGATACCGCTGATTTCAGCCAg > 1:1148283/1‑43 (MQ=255)
tGGTTTCTTTACCGAAGGAGACGATACCGCTGATTTCAGCCAg > 1:274277/1‑43 (MQ=255)
tGGTTTCTTTACCGAAGGAGACGATACCGCTGATTTCAGCCAg > 1:2390342/1‑43 (MQ=255)
tGGTTTCTTTACCGAAGGAGACGATACCGCTGATTTCAGCCAg > 1:1828838/1‑43 (MQ=255)
tGGTTTCTTTACCGAAGGAGACGATACCGCTGATTTCAGCCAGGATTGCCGGCTCTTTCGGACGACGTg < 1:3510285/69‑1 (MQ=255)
tGGTTTCTTTACCGAAGGAGACGATACCGCTGATTTCAGCCAGGATTGCCGGCTCTTTCGGACGACGTg < 1:2287266/69‑1 (MQ=255)
tGGTTTCTTTACCGAAGGAGACGATACCGCTGATTTCAGCCAGGATTGCCGGCTCTTTCGGACGACGTg < 1:1957149/69‑1 (MQ=255)
tGGTTTCTTTACCGAAGGAGACGATACCGCTGATTTCAGCCAGGATTGCCGGCTCTTTCGGACGACGTg < 1:1817255/69‑1 (MQ=255)
tGGTTTCTTTACCGAAGGAGACGATACCGCTGATTTCAGCCAGGATTGCCGGCTCTTTCGGACGACGTGc < 1:2641558/70‑1 (MQ=255)
tGGTTTCTTTACCGAAGGAGACGATACCGCTGATTTCAGCCAGGATTGCCGGCTCTTTCGGACGACGTGc < 1:1353769/70‑1 (MQ=255)
tGGTTTCTTTACCGAAGGAGACGATACCGCTGATTTCAGCCAGGATTGCCGGCTCTTTCGGACGACGTGc < 1:1452786/70‑1 (MQ=255)
tGGTTTCTTTACCGAAGGAGACGATACCGCTGATTTCAGCCAGGATTGCCGGCTCTTTCGGACGACGTGc < 1:2359504/70‑1 (MQ=255)
tGGTTTCTTTACCGAAGGAGACGATACCGCTGATTTCAGCCAGGATTGCCGGCTCTTTCGGACGACGTGc < 1:1631987/70‑1 (MQ=255)
ttCTTTACCGAAGGAGACGATACCGCTGATTTCAGCCAGGATTGCCGGCTCTTTCGGACGACGTGCTTCg > 1:2208611/1‑70 (MQ=255)
ttCTTTACCGAAGGAGACGATACCGCTGATTTCAGCCAGGATTGCCGGCTCTTTCGGACGACGTGCTTCg > 1:3268019/1‑70 (MQ=255)
tACCGAAGGAGACGATACCGCTGATTTCAGCCAGGATTGCCGGct < 1:2422691/45‑1 (MQ=255)
tACCGAAGGAGACGATACCGCTGATTTCAGCCAGGATTGCCGGct < 1:1339128/45‑1 (MQ=255)
aCCGAAGGAGACGATACCGCTGATTTCAGCCAGGATTGCCGGCTCTTTCGGACGACGTGCTTCGAACAgg < 1:3496965/70‑1 (MQ=255)
ccGAAGGAGACGATACCGCTGATTTCAGCCAGGATTGCCGGCTCTTTCGGACGACGTGc < 1:2876473/59‑1 (MQ=255)
cGAAGGAGACGATACCGCTGATTTCAGCCAGGATTGCCGGCTCtt < 1:669025/45‑1 (MQ=255)
|
ACGGATCGCTACCGTCTACCGGGGTGATAACCAGACGACGTTTACCTTTGGTTTCTTTACCGAAGGAAACGATACCGCTGATTTCAGCCAGGATTGCCGGCTCTTTCGGACGACGTGCTTCGAACAGG > W3110S.gb/3447776‑3447903
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
