Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F8 I0 R2
|
296 |
77.6 |
4260404 |
88.1% |
3753415 |
61.2 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
623,375 |
C→T |
67.6% |
T19I (ACC→ATC) |
msbA → |
fused lipid transporter subunits and membrane component and ATP‑binding component of ABC superfamily |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 623,375 | 0 | C | T | 67.6%
| 73.7
/ 55.9
| 63 | T19I (ACC→ATC) | msbA | fused lipid transporter subunits and membrane component and ATP‑binding component of ABC superfamily |
| Reads supporting (aligned to +/- strand): ref base C (14/6); new base T (30/12); total (45/18) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
ATGCTGGTTTTTTGAATGCATAACGACAAAGATCTCTCTACGTGGCAGACATTCCGCCGACTGTGGCCAACCATTGCGCCTTTCAAAGCGGGTCTGATCGTGGCGGGCGTAGCGTTAATCCTCAACGCAGCCAGC > minE/623305‑623439
|
aTGCTGGTTTTTTGAATGCATAACGACAAAGATCTCTCTACGTGGCAGACATTCCGCCGACTGTGGCCAAc > 1:1076211/1‑71 (MQ=255)
aTGCTGGTTTTTTGAATGCATAACGACAAAGATCTCTCTACGTGGCAGACATTCCGCCGACTGTGGCCAAc > 1:1234952/1‑71 (MQ=255)
aTGCTGGTTTTTTGAATGCATAACGACAAAGATCTCTCTACGTGGCAGACATTCCGCCGACTGTGGCCAAc > 1:4187498/1‑71 (MQ=255)
aTGCTGGTTTTTTGAATGCATAACGACAAAGATCTCTCTACGTGGCAGACATTCCGCCGACTGTGGCCAAc > 1:1312927/1‑71 (MQ=255)
aTGCTGGTTTTTTGAATGCATAACGACAAAGATCTCTCTACGTGGCAGACATTCCGCCGACTGTGGCCAAc < 1:1799587/71‑1 (MQ=255)
aTGCTGGTTTTTTGAATGCATAACGACAAAGATCTCTCTACGTGGCAGACATTCCGCCGACTGTGGCCAAc > 1:893468/1‑71 (MQ=255)
tGCTGGTTTTTTGAATGCATAACGACAAAGATCTCTCTACGTGGCAGACATTCCGCCGACTGTGGCCAAcc < 1:1687833/71‑1 (MQ=255)
cTGGTTTTTTGAATGCATAACGACAAAGATCTCTCTACGTGGCAGACATTCCGCCGACTGTGGCCAATCAt > 1:29049/1‑71 (MQ=255)
cTGGTTTTTTGAATGCATAACGACAAAGATCTCTCTACGTGGCAGACATTCCGCCGACTGTGGCCAATCAt > 1:2842070/1‑71 (MQ=255)
cTGGTTTTTTGAATGCATAACGACAAAGATCTCTCTACGTGGCAGACATTCCGCCGACTGTGGCCAATCAt > 1:2216433/1‑71 (MQ=255)
tttGAATGGATAACGACAAAGATCTCTCTACGTGGCAGACATTCCGCCGACTGTGGCCAATCATTGCGCCt > 1:2303158/1‑71 (MQ=255)
tttGAATGCATAACGACAAAGATCTCTCTACGTGGCAGACATTCCGCCGACTGTGGCCAATCATTGCGCCt > 1:1578915/1‑71 (MQ=255)
tttGAATGCATAACGACAAAGATCTCTCTACGTGGCAGACATTCCGCCGACTGTGGCCAATCATTGCGCCt > 1:700961/1‑71 (MQ=255)
tttGAATGCATAACGACAAAGATCTCTCTACGTGGCAGACATTCCGCCGACTGTGGCCAATCATTGCGCCt > 1:564524/1‑71 (MQ=255)
tttGAATGCATAACGACAAAGATCTCTCTACGTGGCAGACATTCCGCCGACTGTGGCCAATCATTGCGCCt > 1:346974/1‑71 (MQ=255)
tttGAATGCATAACGACAAAGATCTCTCTACGTGGCAGACATTCCGCCGACTGTGGCCAATCATTGCGCCt > 1:3658607/1‑71 (MQ=255)
ttGAATGCATAACGACAAAGATCTCTCTACGTGGCAGACATTCCGCCGACTGTGGCCAACCATTGCGCCt < 1:2527960/70‑1 (MQ=255)
cATAACGACAAAGATCTCTCTACGTGGGAGACATTCCGCCGACTGTGGCCAATCATTGCGCCTTTCAAAGc > 1:3701355/1‑71 (MQ=255)
cATAACGACAAAGATCTCTCTACGTGGCAGACATTCCGCCGACTGTGGCCAATCATTGCGCCTTTCAAAg > 1:3009296/1‑70 (MQ=255)
cATAACGACAAAGATCTCTCTACGTGGCAGACATTCCGCCGACTGTGGCCAATCATTGCGCCTTTCAAAGc > 1:3587444/1‑71 (MQ=255)
cATAACGACAAAGATCTCTCTACGTGGCAGACATTCCGCCGACTGTGGCCAATCATTGCGCCTTTCAAAGc > 1:3906961/1‑71 (MQ=255)
cATAACGACAAAGATCTCTCTACGTGGCAGACATTCCGCCGACTGTGGCCAATCATTGCGCCTTTCAAAGc < 1:2674779/71‑1 (MQ=255)
cATAACGACAAAGATCTCTCTACGTGGCAGACATTCCGCCGACTGTGGCCAATCATTGCGCCTTTCAAAGc > 1:808810/1‑71 (MQ=255)
aaTAACGACAAAGATCTCTCTACGTGGCAGACATTCCGCCGACTGTGGCCAATCATTGCGCCTTTCAAAGc > 1:1257852/2‑71 (MQ=255)
tAACGACAAAGATCTCTCTACGTGGCAGACATTCCGCCGACTGTGGCCAATCa < 1:1615868/53‑1 (MQ=255)
aCGACAAAGATCTCTCTACGTGGCAGACATTCCGCCGACTGTGGCCAAc > 1:3084136/1‑49 (MQ=255)
aCGACAAAGATCTCTCTACGTGGCAGACATTCCGCCGACTGTGGCCAAc > 1:3078786/1‑49 (MQ=255)
aCGACAAAGATCTCTCTACGTGGCAGACATTCCGCCGACTGTGGCCAATCATTgcgc > 1:2676048/1‑57 (MQ=255)
aCGACAAAGATCTCTCTACGTGGCAGACATTCCGCCGACTGTGGCCAATCATTg > 1:3221269/1‑54 (MQ=255)
aCGACAAAGATCTCTCTACGTGGCAGACATTCCGCCGACTGTGGCCAATCATTg > 1:3640869/1‑54 (MQ=255)
gACAAAGATCTCTCTACGTGGCAGACATTCCGCCGACTGTGGCCAACCATTGCGCCTTTCAAAGc > 1:4193730/1‑65 (MQ=255)
gACAAAGATCTCTCTACGTGGCAGACATTCCGCCGACTGTGGCCAACCATTGCGCCTTTCAAAGCGGGTCt > 1:1719779/1‑71 (MQ=255)
gACAAAGATCTCTCTACGTGGCAGACATTCCGCCGACTGTGGCCAACCATTGCGCATTTCAAAGCGGGTCt > 1:3990834/1‑71 (MQ=255)
aaGATCTCTCTACGTGGCAGACATTCCGCCGACTGTGGCCAATCATTGCGCCTTTCAAAGCGGGTCTGATc > 1:98805/1‑71 (MQ=255)
aaGATCTCTCTACGTGGCAGACATTCCGCCGACTGTGGCCAATCATTGCGCCTTTCAAAGCGGGTCTGATc > 1:4074037/1‑71 (MQ=255)
aaGATCTCTCTACGTGGCAGACATTCCGCCGACTGTGGCCAATCATTGCGCCTTTCAAAGCGGGTCTGATc > 1:3458174/1‑71 (MQ=255)
gATCTCTCTACGTGGCAGACATTCCGCCGACTGTGGCCAACCATTGCGCCTTTCAAAGCGGGTCTGATCGt < 1:2464907/71‑1 (MQ=255)
ctACGTGGCAGACATTCCGCCGACTGTGGCCAATCATTGCGCCTTTCAAAGCGGGTCTGATCGTggc < 1:954459/67‑1 (MQ=255)
tACGTGGCAGACATTCCGCCGACTGTGGCCAACCATTGCGCCTTTc > 1:4088482/1‑46 (MQ=255)
tACGTGGCAGACATTCCGCCGACTGTGGCCAACCATTGCGCCTTTCAAAGCGGGTCTGATCGTggcgggcg > 1:2860147/1‑71 (MQ=255)
tACGTGGCAGACATTCCGCCGACTGTGGACAATCATTGCGCCTTTCAAAGCGGGTCTGATCGTggcgggcg > 1:316824/1‑71 (MQ=255)
gTGGCAGACATTCCGCCGACTGTGGCCAATCATTGCGCCTTTCAAAGCGGGTCTGATCGTGGCGGGCGTAg < 1:3479223/71‑1 (MQ=255)
gTGGCAGACATTCCGCCGACTGTGGCCAATCATTGCGCCTTTCAAAGCGGGTCTGATCGTGGCGGGCGTAg < 1:1159562/71‑1 (MQ=255)
gTGGCAGACATTCCGCCGACTGTGGCCAACCATTGCGCCTTTCAAAGCGGGTCTGATCGTGGCGGGCGTAg > 1:4130883/1‑71 (MQ=255)
cAGACATTCCGCCGACTGTGGCCAATCATTGCGCCTTTCAAAGCGGGTCTGATCGTGGCGGGCGTAGCGtt < 1:1613261/71‑1 (MQ=255)
aGACATTCCGCCGACTGTGGCCAATCATTGCGCCTTTCAAAGCGGGTCTGATCGTGGCGGGCGTAGCGtt < 1:2406333/70‑1 (MQ=255)
aGACATTCCGCCGACTGTGGCCAATCATTGCGCCTTTCAAAGCGGGTCTGATCGTGGCGGGCGTAGCGTTa < 1:3965867/71‑1 (MQ=255)
aGACATTCCGCCGACTGTGGCCAAACATTGCGCCTTTCAAAGCGGGTCTGATCGTgg > 1:1737833/1‑57 (MQ=255)
aCATTCCGCCGACTGTGGCCAATCATTGCGCCTTTCAAAGCGGGTCTGATCGTGGCGGGCGTAGCGTTAAt > 1:3884939/1‑71 (MQ=255)
aCATTCCGCCGACTGTGGCCAACCATTGCGCCTTTCAAAGCGGGTCTGATCGTGGCGGGCGTAGCGTTAAt > 1:1517420/1‑71 (MQ=255)
aCATTCCGCCGACTGTGGCCAACCATTGCGCCTTTCAAAGCGGGTCTGATCGTGGCGGGCGTAGCGTTAAt > 1:1846560/1‑71 (MQ=255)
aCATTCCGCCGACTGTGGCCAACCATTGCGCCTTTCAAAGCGGGTCTGATCGTGGCGGGCGTAGCGTTAAt > 1:1847693/1‑71 (MQ=255)
aCATTCCGCCGACTGTGGCCAACCATTGCGCCTTTCAAAGCGGGTCTGATCGTGGCGGGCGTAGCGTTAAt > 1:785582/1‑71 (MQ=255)
cATTCCGCCGACTGTGGCCAATCATTGCGCCTTTCAAAGCGGGTCTGATCGTGGCGGGCGTAGCGTTAATc < 1:297253/71‑1 (MQ=255)
aTTCCGGCGACTGTGGCCAATCATTGCGCCTTTCAAAGCGGGTCTGATCGTGGCGGGCGTAGCGTTAATcc > 1:540772/1‑71 (MQ=255)
aTTCCGCCGACTGTGGCCAATCATTGCGCCTTTCAAAGCGGGTCTGATGGTGGCGGGCGTAGCGTTAATcc > 1:2159995/1‑71 (MQ=255)
aTTCCGCCGACTGTGGCCAATCATTGCGCCTTTCAAAGCGGGTCTGATCGTGGCGGGCGTAGCGTTAATcc > 1:3010453/1‑71 (MQ=255)
aTTCCGCCGACTGTGGCCAACCATTGCGCCTTTCAAAGCGGGTCTGATCGTGGCGGGCGTAGCGTTAAt > 1:1688822/1‑69 (MQ=255)
aTTCCGCCGACTGTGGCCAACCATTGCGCCTTTCAAAGCGGGTCTGATCGTGGCGGGCGTAGCGTTAAt > 1:1693549/1‑69 (MQ=255)
tCCGCCGACTGTGGCCAATCATTGCGCCTTTCAAAg > 1:1677659/1‑36 (MQ=255)
tCCGCCGACTGTGGCCAATCATTGCGCCTTTCAAAg > 1:3263746/1‑36 (MQ=255)
ccgccgACTGTGGCCAATCATTGCGCCTTTCAAAg > 1:2061519/1‑35 (MQ=255)
ccgccgACTGTGGCCAATCATTGCGCCTTTCAAAg > 1:3741719/1‑35 (MQ=255)
cgccgACTGTGGCCAATCATTGCGCCTTTCAAAGCGGGTCTGATCGTGGCGGGCGTAGCGTTAATCCTCa < 1:3518362/70‑1 (MQ=255)
cgccgACTGTGGCCAATCATTGCGCCTTTCAAAGCGAGTCTGATCGTGGCGGGCGTAGCGTTAATCCTCaa < 1:197374/71‑1 (MQ=255)
gccgACTGTGGCCAATCATTGCGCCTTTCAAAGCGGGTCTGATCGTGGCGGGCGTAGCGTTAATACTCaa < 1:4197124/70‑1 (MQ=255)
cgACTGTGGCCAACCATTGCGCCTTTCAAAGCGGGTCTGATCGTGGCGGGCGTAGCGTTAATCCTCAACGc < 1:4221783/71‑1 (MQ=255)
cgACTGTGGCCAACCATTGCGCCTTTCAAAGCGGGTCTGATCGTGGCGGGCGTAGCGTTAATCCTCAACGc < 1:2152337/71‑1 (MQ=255)
tgtgGCCAACCATTGCGCCTTTCAAAGCGGGTCTGATCGTGGCGGGCGTAGCGTTAATCCTCAACGcagc > 1:1628822/1‑70 (MQ=255)
tgtgGCCAACCATTGCGCCTTTCAAAGCGGGTCTGATCGTGGCGGGCGTAGCGTTAATCCTCAACGcagc > 1:3399556/1‑70 (MQ=255)
tggcAACCATTGCGCCTTTCAAAGCGGGTCTGATCGTGGCGGGCGTAGCGTTAATCCTCAACGcagccagc < 1:3842937/68‑1 (MQ=255)
gCCAACCATTGCGCCTTTCAAAGCGGGTCTGATCGTGGCGGGCGTAGCGTTAATCCTCAACGcagccagc < 1:2939662/70‑1 (MQ=255)
|
ATGCTGGTTTTTTGAATGCATAACGACAAAGATCTCTCTACGTGGCAGACATTCCGCCGACTGTGGCCAACCATTGCGCCTTTCAAAGCGGGTCTGATCGTGGCGGGCGTAGCGTTAATCCTCAACGCAGCCAGC > minE/623305‑623439
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
