Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F8 I0 R2
|
296 |
77.6 |
4260404 |
88.1% |
3753415 |
61.2 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
1,408,722 |
G→A |
35.0% |
A331T (GCG→ACG) |
yejF → |
fused predicted oligopeptide transporter subunits and ATP‑binding components of ABC superfamily |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 1,408,722 | 0 | G | A | 35.0%
| 45.9
/ 34.3
| 40 | A331T (GCG→ACG) | yejF | fused predicted oligopeptide transporter subunits and ATP‑binding components of ABC superfamily |
| Reads supporting (aligned to +/- strand): ref base G (10/16); new base A (8/6); total (18/22) |
| Fisher's exact test for biased strand distribution p-value = 3.27e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
GCTACGAGCGGGTGAAACACTGGGTTTAGTGGGCGAGTCCGGTTCCGGGAAAAGTACGACGGGACTGGCGCTGCTGCGACTGATTAATTCTCAGGGCAGCATCATCTTTGACGGTCAGCCACTGCAAAATTTAA > minE/1408655‑1408788
|
gCTACGAGCGGGTGAAACACTGGGTTTAGTGGGCGAGTCCGGTTCCGGGAAAAGTACGACGGGACTGacgc < 1:2800864/71‑1 (MQ=255)
cGAGCGGGTGAAACACTGGGTTTAGTGGGCGAGTCCGGTTCCGGGAAAAGTACGACGGGACTGACgctgct > 1:4182283/1‑71 (MQ=255)
gggTGAAACACTGGGTTTAGTGGGCGAGTCCGGTTCCGGGAAAAGTACGACGGGACTGGCGCTGCTGCGAc > 1:3648874/1‑71 (MQ=255)
acTGGGTTTAGTGGGCGAGTCCGGTTCCGGGAAAAGTACGACGGGACTGGCGCTGCTGCGACTGATTAAtt < 1:2639982/71‑1 (MQ=255)
cTGGGTTTAGTGGGCGAGTCCGGTTCCGGGAAAAGTACGACGGGACTGGCGCTGCTGCGACTGATTAATtc < 1:4025108/71‑1 (MQ=255)
gggTTTAGTGGGCGAGTCCGGTTCCGGGAAAAGTACGACGGGACTGGCGCTGCTGCGACTGATTAATtct > 1:467470/1‑70 (MQ=255)
ggggCGAGTCCGGTTCCGGGAAAAGTACGACGGGACTGGCGCTGCTGCGACTGATTAATtc < 1:2161224/60‑1 (MQ=255)
ggCGAGTCCGGTACCGGGAAAAGTACGACGGGACTGACGCTGCTGCGACTGATTAATTCTCAGGGCAGcat > 1:1034599/1‑71 (MQ=255)
ggCGAGTCCGGGTCCGGGAAAAGTACGACGGGACTGACGCTGCTGCGACTGATTAATTCTCAGGGCAGcat > 1:3651468/1‑71 (MQ=255)
cGGTTCCGGGAAAAGTACGACGGGACTGGCGCTGCTGCGACTGATTAATTCTCAGGGCAGCATCATCTTTg < 1:907432/71‑1 (MQ=255)
cGGTTCCGGGAAAAGTACGACGGGACTGGCGCTGCTGCGACTGATTAATTCTCAGGGCAGCATCATCTTTg < 1:1313264/71‑1 (MQ=255)
ccGGGAAAAGTACGACGGGACTGGCGCTGCTGCGACTGATTAATTCTCAGGGCAGCATCATCTTTGACGGt > 1:2321671/1‑71 (MQ=255)
ccGGGAAAAGTACGACGGGACTGGCGCTGCTGCGACTGATTAATTCTCAGGGCAGCATCATCTTTGACGGt > 1:2465670/1‑71 (MQ=255)
ccGGGAAAAGTACGACGGGACTGGCGCTGCTGCGACTGATTAATTCTCAGGGCAGCATCATCTTTGACGGt > 1:787297/1‑71 (MQ=255)
ccGGGAAAAGTACGACGGGACTGGCGCTGCTGCGACTGATTAATTCTCAGGGCAGCATCATCTTTGACGGt > 1:496580/1‑71 (MQ=255)
gggAAAAGTACGACGGGACTGGCGCTGCTGCGACTGATTAATTCTCAGGGCAGcatc < 1:387004/57‑1 (MQ=255)
gAAAAGTACGACGGGACTGGCGCTGCTGCGACTGATTAATtc > 1:3739460/1‑42 (MQ=255)
gAAAAGTACGACGGGACTGGCGCTGCTGCGACTGATTAATTCTCAGGGCAGcatcat > 1:1759228/1‑57 (MQ=255)
gAAAAGTACGACGGGACTGGCGCTGCTGCGACTGATTAATTCTCAGGGCAGcatcat > 1:1709955/1‑57 (MQ=255)
gAAAAGTACGACGGGACTGGCGCTGCTGCGACTGATTAATTCTCAGGGCAGcatcat > 1:435666/1‑57 (MQ=255)
gAAAAGTACGACGGGACTGGCGCTGCTGCGACTGATTAATTCTCAGGGCAGCATCATCTTTGACGGTCAGc < 1:25716/71‑1 (MQ=255)
gAAAAGTACGACGGGACTGACGCTGCTGCGACTGATTAATTCTCAGGGCAGCATCATCTTTGACGGTCAGc > 1:1024645/1‑71 (MQ=255)
aaaaGTACGACGGGACTGGCGCTGCTGCGACTGATTAATTCTCAGGGCAGCATCATCTTTGACGGTCAGcc < 1:4205840/71‑1 (MQ=255)
aaaaGTACGACGGGACTGGCGCTGCTGCGACTGATTAATTCTCAGGGCAGCATCATCTTTGACGGTCAGcc < 1:830111/71‑1 (MQ=255)
acgacgGGACTGACGCTGCTGCGACTGATTAATTCTCAGGGCAGCATCATCTTTGa > 1:1011355/1‑56 (MQ=255)
acgacgGGACTGACGCTGCTGCGACTGATTAATTCTCAGGGCAGCATCATCTTTGa > 1:4029728/1‑56 (MQ=255)
acgacgGGACTGACGCTGCTGCGACTGATTAATTCTCAGGGCAGCATCATCTTTGa > 1:4251111/1‑56 (MQ=255)
acgacgGGACTGACGCTGCTGCGACTGATTAATTCTCAGGGCAGCATCATCTTTGa > 1:3807940/1‑56 (MQ=255)
cgacgGGACTGGCGCTGCTGCGACTGATTAATTCTCAGGGCAGCATCATCTTTGACGGTCAGCCACTGc < 1:3949744/69‑1 (MQ=255)
cgacgGGACTGGCGCTGCTGCGACTGATTAATTCTCAGGGCAGCATCATCTTTGACGGTCAGCCACTGc < 1:4143515/69‑1 (MQ=255)
cgacgGGACTGACGCTGCTGCGACTGATTAATTCTCAGGGCAGCATCATCTTTGACGGTCAGCCACTGCaa < 1:1272137/71‑1 (MQ=255)
cgacgGGACTGACGCTGCTGCGACTGATTAATTCTCAGGGCAGCATCATCTTTGACGGTCAGCCACTGCaa < 1:4033570/71‑1 (MQ=255)
gggACTGACGCTGCTGCGACTGATTAATTCTCAGGGCAGCATCATCTTTGACGGTCAGCCACTGCAAAAtt < 1:2202081/71‑1 (MQ=255)
gggACTGACGCTGCTGCGACTGATTAATTCTCAGGGCAGCATCATCTTTGACGGTCAACCACTGCAAAAtt < 1:367070/71‑1 (MQ=255)
ggACTGACGCTGCTGCGACTGATTAATTCTCAGGGCAGCATCATCTTTGACGGTCAGCCACTGCAAAAttt < 1:1805829/71‑1 (MQ=255)
ggACTGACGCTGCTGCGACTGATTAATTCTCAGGGCAGCATCATCTTTGACGGTCAGCCACTGCAAAAttt < 1:727758/71‑1 (MQ=255)
aCTGGCGCTGCTGCGACTGATTAATTCTCAGGGCAGCATCATCTTTGACGGTCAGCCACTGCAAAATTTaa < 1:1348707/71‑1 (MQ=255)
aCTGGCGCTGCTGCGACTGATTAATTCTCAGGGCAGCATCATCTTTGACGGTCAGCCACTGCAAAATTTaa < 1:3191514/71‑1 (MQ=255)
aCTGGCGCTGCTGCGACTGATTAATTCTCAGGGCAGCATCATCTTTGACGGTCAGCCACTGCAAAATTTaa < 1:1736644/71‑1 (MQ=255)
aCTGGCGCTGCTGCGACTGATTAATTCTCAGGGCAGCATCATCTTTGACGGTCAGCCACTGCAAAATTTaa < 1:2772017/71‑1 (MQ=255)
aCTGGCGCTGATGCGACTGATTAATTCTCAGGGCAGCATCATCTTTGACGGTCAGCCACTGCAAAATTTaa < 1:4069688/71‑1 (MQ=255)
|
GCTACGAGCGGGTGAAACACTGGGTTTAGTGGGCGAGTCCGGTTCCGGGAAAAGTACGACGGGACTGGCGCTGCTGCGACTGATTAATTCTCAGGGCAGCATCATCTTTGACGGTCAGCCACTGCAAAATTTAA > minE/1408655‑1408788
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
