Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F8 I0 R2
|
296 |
77.6 |
4260404 |
88.1% |
3753415 |
61.2 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
1,475,665 |
A→T |
48.6% |
T244S (ACC→TCC) |
nrdB → |
ribonucleoside diphosphate reductase 1, beta subunit, ferritin‑like |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 1,475,665 | 0 | A | T | 48.6%
| ‑5.9
/ 103.4
| 70 | T244S (ACC→TCC) | nrdB | ribonucleoside diphosphate reductase 1, beta subunit, ferritin‑like |
| Reads supporting (aligned to +/- strand): ref base A (27/9); new base T (18/16); total (45/25) |
| Fisher's exact test for biased strand distribution p-value = 8.05e-02 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.50e-01 |
GCGAATTGATGGAAGGCAACGCCAAAATTATTCGCCTGATTGCCCGCGACGAAGCCCTGCACCTGACCGGCACCCAGCATATGCTGAATCTGCTGCGCAGCGGCGCGGACGATCCTGAGATGGCGGAAAT > minE/1475600‑1475729
|
gcgAATTGATGGAAGGCAACGCCAAAATTATTCGCCTGATTGCCCGCGACGAAGCCCTGCACCTGTCCGGc < 1:2520440/71‑1 (MQ=255)
gcgAATTGATGGAAGGCAACGCCAAAATTATTCGCCTGATTGCCCGCGACGAAGCCCTGCACCTGTCCGGc < 1:2700037/71‑1 (MQ=255)
gATGGAAGGCAACGCCAAAATTATTCGCCTGATTGCCCGCGACGAAGCCCTGCACCTGACCGGCACCCAg < 1:277064/70‑1 (MQ=255)
aTGGAAGGCAACGCCAAAATTATTCGCCTGATTGCCCGCGACGAAGCCCTGCACCTGTCCGGCACCCAGCa < 1:415052/71‑1 (MQ=255)
aTGGAAGGCAACGCCAAAATTATTCGCCTGATTGCCCGCGACGAAGCCCTGCACCTGTCCGGCACCCAGCa < 1:4196738/71‑1 (MQ=255)
aTGGAAGGCAACGCCAAAATTATTCGCCTGATTGCCCGCGACGAAGCCCTGCACCTGACCGGCACCCAGCa < 1:3090789/71‑1 (MQ=255)
gCAACGCCAAAATTATTCGCCTGATTGCCCGCGACGAAGCCCTGCACCTGTCCGGCACCCAGCATATGCt > 1:2192071/1‑70 (MQ=255)
cGCCGAAATTATTCGCCTGATTGCCCGCGACGAAGCCCTGCACCTTTCCGGCACCCAGc < 1:3071682/59‑1 (MQ=255)
cGCCAAAATTATTCGCCTGATTGCCCGCGACGAAGCCCTGCACCTGTCCGGCACCCAGc < 1:1201954/59‑1 (MQ=255)
gCCAAAATTATTCGCCTGATTGCCCGCGACGAAGCCCTGCACCTGTCCGGCACCCCGCata > 1:2737361/1‑61 (MQ=255)
gCCAAAATTATTCGCCTGATTGCCCGCGACGAAGCCCTGCACCTGTCCGGCACCCAGCata > 1:3540688/1‑61 (MQ=255)
gCCAAAATTATTCGCCTGATTGCCCGCGACGAAGCCCTGCACCTGTCCGGCACCCAGCata > 1:2479399/1‑61 (MQ=255)
gCCAAAATTATTCGCCTGATTGCCCGCGACGAAGCCCTGCACCTGTCCGGCACCCAGCata > 1:369456/1‑61 (MQ=255)
gCCAAAATTATTCGCCTGATTGCCCGCGACGAAGCCCTGCACCTGTCCGGCACCCAGCATATGCTGAATct > 1:2227613/1‑71 (MQ=255)
ccAAAATTATTCGCCTGATTGCCCGCGACGAAGCCCTGCACCTGTCCGGCACCCAGCATATGCTGAATctg > 1:351621/1‑71 (MQ=255)
ccAAAATTATTCGCCTGATTGCCCGCGACGAAGCCCTGCACCTGACCGGCACCCAGCATATGCTGAATctg > 1:364272/1‑71 (MQ=255)
aaaaTTATTCGCCTGATTGCCCGCGACGAAGCCCTGCACCTGTCCGGCACCCAGCATATGCTGAATctgct < 1:956886/71‑1 (MQ=255)
ttattCGCCTGATTGCCCGCGACGAAGCCCTGCACCTGTCCGGCACCCAGCATATGCTGAATCTGCTgcgc < 1:2074900/71‑1 (MQ=255)
ttCGCCTGATTGCCTGCGACGAAGCCCTGCACCTGACCGGCACCCAGCATATGCTGAATCTGCTGCGCAgc < 1:1520514/71‑1 (MQ=255)
ttCGCCTGATTGCCCGCGACGAAGCCCTGCACCTGTCCGGCACCCAGCATATGCTGAATCTGCTGCGCAg < 1:2620987/70‑1 (MQ=255)
cGCCTGATTGCCCGCGCCGAAGCCCTGCACCTGTCCGGCCCCCAGCATATGCTGAATCTGCTGCGCAg > 1:1852713/1‑68 (MQ=255)
cGCCTGATTGCCCGCGACGAAGCCCTGCACCTGTCCGGCACCCAGCATATGCTGAATCTGCTGCGCAg > 1:1526752/1‑68 (MQ=255)
cGCCTGATTGCCCGCGACGAAGCCCTGCACCTGTCCGGCACCCAGCATATGCTGAATCTGCTGCGCAg > 1:70061/1‑68 (MQ=255)
cGCCTGATTGCCCGCGACGAAGCCCTGCACCTGTCCGGCACCCAGCATATGCTGAATCTGCTGCGCAg > 1:1289253/1‑68 (MQ=255)
ccTGATTGCCCGCGACGAAGCCCTGCACCTGTCCGGc < 1:3936258/37‑1 (MQ=255)
tGATTGCCCGCGACGAAGCCCTGCACCTGTCCGGCACCCAGCATATGCTGAATCTGCTgcgc < 1:2369457/62‑1 (MQ=255)
tGATTGCCCGCGACGAAGCCCTGCACCTGACCGGCACCCAGCATATGCTGAATCTGCTGCGCAGCGGcgcg > 1:3310229/1‑71 (MQ=255)
tGATTGCCCGCGACGAAGCCCTGCACCTGACCGGCACCCAGCATATGCTGAATCTGCTGCGCAGCGGcgcg > 1:1212557/1‑71 (MQ=255)
gATTGCCCGCGACGAAGCCCTGCACCTGTCCGGCACCCAGCATATGCTGAATCTGCTGCGCAg < 1:3012560/63‑1 (MQ=255)
gATTGCCCGCGACGAAGCCCTGCACCTGTCCGGCACCCAGCATATGCTGAATCTGCTGCGCAGCGGcgcg > 1:2250382/1‑70 (MQ=255)
gATTGCCCGCGACGAAGCCCTGCACCTGTCCGGCACCCAGCATATGCTGAATCTGCTGCGCAGCGGcgcg > 1:455992/1‑70 (MQ=255)
gATTGCCCGCGACGAAGCCCTGCACCTGTCCGGCACCCAGCATATGCTGAATCTGCTGCGCAGCGGcgcg > 1:3875364/1‑70 (MQ=255)
gATTGCCCGCGACGAAGCCCTGCACCTGTCCGGCACCCAGCATATGCTGAATCTGCTGCGCAGCGGcgcg > 1:2743611/1‑70 (MQ=255)
cccGCGACGAAGCCCTGCACCTGTCCGGCACCCAGCATATGCTGAATCTGCTGCGCAGCGGCGCGGACGa < 1:200174/70‑1 (MQ=255)
cccGCGACGAAGCCCTGCACCTGTCCGGCACCCAGCATATGCTGAATCTGCTGCGCAGCGGCGCGGACGa < 1:144582/70‑1 (MQ=255)
cccGCGACGAAGCCCTGCACCTGTCCGGCACCCAGCATATGCTGAATCTGCTGCGCAGCGGCGCGGACGAt < 1:2978092/71‑1 (MQ=255)
cccGCGACGAAGCCCTGCACCTGACCGGCACCCAGCATATGCTGAATCTGCTGCGCAGCGGCGCGGACGa < 1:3876784/70‑1 (MQ=255)
cgcgACGAAGCCCTGCACCTGTCCGGCACCCAGCATATGCTGAATCTGCTGCGCAg < 1:222416/56‑1 (MQ=255)
gcgACGAAGCCCTGCACCTGACCGGCACCCAGCATATGCTGAATCTGCTGCGCAGCGGCGCGGACGATCCt > 1:4066903/1‑71 (MQ=255)
gacgaAGCCCTGCACCTGACCGGCACCCAGCATATGCTGAATCTGCTGCGCAGCGGCGCGGACGATCCt > 1:324433/1‑69 (MQ=255)
gacgaAGCCCTGCACCTGACCGGCACCCAGCATATGCTGAATCTGCTGCGCAGCGGCGCGGACGATCCt > 1:922770/1‑69 (MQ=255)
gacgaAGCCCTGCACCTGACCGGCACCCAGCATATGCTGAATCTGCTGCGCAGCGGCGCGGACGATCCt > 1:541663/1‑69 (MQ=255)
gacgaAGCCCTGCACCTGACCGGCACCCAGCATATGCTGAATCTGCTGCGCAGCGGCGCGGACGATCCTga < 1:1198786/71‑1 (MQ=255)
gacgaAGCCCTGCACCTGACCGGCACCCAGCATAAGCTGAATCTGCTGCGCAGCGGCGCGGACGATCCTg > 1:2690541/1‑70 (MQ=255)
cgaAGCCCTGCACCTGACCGGCACCCAGCATATGCTGAATCTGCTGCGCAGCGGCGCGGACg > 1:3437178/1‑62 (MQ=255)
cgaAGCCCTGCACCTGACCGGCACCCAGCATATGCTGAATCTGCTGCGCAGCGGCGCGGACGATCCt > 1:2324019/1‑67 (MQ=255)
gaAGCCCTGCACCTGACCGGCACCCAGCATATGCTGAATCTGCTGCGCAGCGGCGCGGACGATCCTga > 1:923235/1‑68 (MQ=255)
gaAGCCCTGCACCTGACCGGCACCCAGCATATGCTGAATCTGCTGCGCAGCGGCGCGGACGATCCTga > 1:1727885/1‑68 (MQ=255)
gaAGCCCTGCACCTGACCGGCACCCAGCATATGCTGAATCTGCTGCGCAGCGGCGCGGACGATCCTga > 1:2170450/1‑68 (MQ=255)
aGCCCTGCACCTGTCCGGCA‑CCAGCATATGCTGAATCTGCTGCGCAGCGGCGCGGACGATCCTGAGATGGc > 1:492917/1‑71 (MQ=255)
cccTGCACCTGACCGGCACCCAGCATATGCTGAATCTGCTGCGCAGCGGCGCGGACGATCCTga > 1:2071647/1‑64 (MQ=255)
cccTGCACCTGACCGGCACCCAGCATATGCTGAATCTGCTGCGCAGCGGCGCGGACGATCCTga > 1:2413883/1‑64 (MQ=255)
cccTGCACCTGACCGGCACCCAGCATATGCTGAATCTGCTGCGCAGCGGCGCGGACGATCCTga > 1:3816489/1‑64 (MQ=255)
cccTGCACCTGACCGGCACCCAGCATATGCTGAATCTGCTGCGCAGCGGCGCGGACGATCCTg > 1:2017773/1‑63 (MQ=255)
ccTGCACCTGACCGGCACCCAGCATATGCTGAATCTGCTGCGCAGCGGCGCGGACGATCCTGAGATGGCg > 1:975543/1‑70 (MQ=255)
cTGCACCTGACCGGCACCCAGCATATGCTGAATCTGCTGCGCAGCGGCGCGGACGATCCTGAGATGGCGGa < 1:1839362/71‑1 (MQ=255)
cTGCACCTGACCGGCACCCAGCATATGCTGAATCTGCTGCGCAGCGGCGCGGACGATCCTGAGATGGCGGa < 1:290611/71‑1 (MQ=255)
cTGCACCTGACCGGCACCCAGCATATGCTGAATCTGCTGCGCAGCGGCGCGGACGATCCTGAGATGGCGGa < 1:295652/71‑1 (MQ=255)
cTGCACCTGACCGGCACCCAGCATATGCTGAATCTGCTGCGCAGCGGCGCGGACGATCCTGAGATGGCGGa < 1:3033548/71‑1 (MQ=255)
gCACCTGACCGGCACCCAGCATATGCTGAATCTGCTGCGCAGCGGCGCGGACGATCCTGAGATGGCGGaaa > 1:1932230/1‑71 (MQ=255)
gCACCTGACCGGCACCCAGCATATGCTGAATCTGCTGCGCAGCGGCGCGGACGATCCTGAGATGGCGGaaa > 1:119628/1‑71 (MQ=255)
gCACCTGACCGGCACCCAGCATATGCTGAATCTGCTGCGCAGCGGCGCGGACGATCCTGAGATGGCGGaaa > 1:30341/1‑71 (MQ=255)
cACCTGTCCGGCACCCAGCATATGCTGAATCTGCTGCGCAGCGGCGCGGACGATCCTGAGATGGCGGAAAt > 1:2701638/1‑71 (MQ=255)
cACCTGTCCGGCAACCAGCATATGCTGAATCTGCTGCGCAGCGGCGCGGACGATCCTGAGATGGCGGAAAt > 1:2737911/1‑71 (MQ=255)
cACCTGACCGGCACCCAGCATATGCTGAATCTGCTGCGCAGCGGCGCGGa > 1:3289723/1‑50 (MQ=255)
cACCTGACCGGCACCCAGCATATGCTGAATCTGCTGCGCAGCGGCGCGGa > 1:1225759/1‑50 (MQ=255)
cACCTGACCGGCACCCAGCATATGCAGAATCTGCTGCGCAGCGGCGCgg > 1:1281017/1‑49 (MQ=255)
tGACCGGCACCCAGCATATGCTGAATCTGCTGCGCAGCGGCGCGGACGATCCTgaga > 1:2846701/1‑57 (MQ=255)
gACCGGCACCCAGCATATGCTGAATCTGCTGCGCAGCGGCGCGGACGATCCTg > 1:1899434/1‑53 (MQ=255)
gACCGGCACCCAGCATATGCTGAATCTGCTGCGCAGCGGCGCGGACGATCCTg > 1:423402/1‑53 (MQ=255)
|
GCGAATTGATGGAAGGCAACGCCAAAATTATTCGCCTGATTGCCCGCGACGAAGCCCTGCACCTGACCGGCACCCAGCATATGCTGAATCTGCTGCGCAGCGGCGCGGACGATCCTGAGATGGCGGAAAT > minE/1475600‑1475729
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
