Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F8 I0 R2
|
296 |
77.6 |
4260404 |
88.1% |
3753415 |
61.2 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
1,533,542 |
G→A |
41.5% |
P51S (CCT→TCT) |
prmB ← |
N5‑glutamine methyltransferase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 1,533,542 | 0 | G | A | 41.5%
| 44.2
/ 75.7
| 65 | P51S (CCT→TCT) | prmB | N5‑glutamine methyltransferase |
| Reads supporting (aligned to +/- strand): ref base G (13/25); new base A (19/8); total (32/33) |
| Fisher's exact test for biased strand distribution p-value = 5.69e-03 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
TTTTTCGCTGGAGGTCAGACGCGCGGTGCGCATATCTTCCGGAATATCCAGCGGCAGGTAGAGCGAAGGCAACACCAGCTGTACGGCTTCATCCCACGGGTTATCGGTACCGTGACCGTACCAGATATTTGCCGCGCTG > minE/1533474‑1533612
|
tttttCGCTGGAGGTCAGACGCGCGGTGCGCATATCTTCCGGAATATCCAGCGGCAGGTAGAGCGAAGGc < 1:67682/70‑1 (MQ=255)
tttCGCTGGAGGTCAGACGCGCGGTGCGCATATCTTCCGGAATATCCAGCGGCAGGTAGAGCGAAGGCaa > 1:3889941/1‑70 (MQ=255)
gCTGGAGGTCAGACGCGCGGTGCGCATATCTTCCGGAATATCCAGCGGCAGGTAGAGCGAAGACAACAcc < 1:2377066/70‑1 (MQ=255)
gTCAGACGCGCGGTGCGCATATCTTCCGGAATATCCAGCGGCAGGTAGAGCGAAGGCAACACCAGCTGt < 1:383872/69‑1 (MQ=255)
gTCAGACGCGCGGTGCGCATATCTTCCGGAATATCCAGCGGCAGGTAGAGCGAAGGCAACACCAGCTGTAc > 1:2801779/1‑71 (MQ=255)
aCGCGCGGTGCGCATATCTTCCGGAATATCCAGCGGCAGGTAGAGCGAAGGCAACACCa < 1:3291962/59‑1 (MQ=255)
gcgcgGTGCGCATATCTTCCGGAATATCCAGCGGCTGGTAGAGCGAAGACAACACCAGCTGTACGGCTTCa > 1:3829431/1‑71 (MQ=255)
gcgcgGTGCGCATATCTTCCGGAATATCCAGCGGCAGGTAGAGCGAAGACAACACCAGCTGTACGGCTTCa > 1:1038266/1‑71 (MQ=255)
ggTGCGCATATCTTCCGGAATATCCAGCGGCAGGTAGAGCGAAGACaa > 1:3371358/1‑48 (MQ=255)
ggTGCGCATATCTTCCGGAATATCCAGCGGCAGGTAGAGCGAAGACAACACCAGCTGTACGGCTTCATcc > 1:3957400/1‑70 (MQ=255)
gcgcATATCTTCCGGAATATCCAGCGGCAGGTAGAGCGAAGGCAACACCAGCTGTACGGCTTCATCCCACg > 1:510103/1‑71 (MQ=255)
cATATCTTCCGGAATATCCAGCGGCAGGTAGAGCGAAGGCAACACCAGCTGTACGGCTTCATCCCACGGGt < 1:1939827/71‑1 (MQ=255)
tatCTTCCGGAATATCCAGCGGCAGGTAGAGCGAAGGCAACACCAGCTGTACGTCtt < 1:456124/57‑1 (MQ=255)
tatCTTCCGGAATATCCAGCGGCAGGTAGAGCGAAGGCAACACCAGCTGTACGGCtt < 1:435640/57‑1 (MQ=255)
tatCTTCCGGAATATCCAGCGGCAGGTAGAGCGAAGGCAACACCAGCTGTACGGCtt < 1:2585017/57‑1 (MQ=255)
tCTTCCGGAATATCCAGCGGCAGGTAGAGCGAAGGCAACACCAGCTGTa > 1:920133/1‑49 (MQ=255)
tCTTCCGGAATATCCAGCGGCAGGTAGAGCGAAGGCAACACCAGCTGTa > 1:3477481/1‑49 (MQ=255)
tCTTCCGGAATATCCAGCGGCAGGTAGAGCGAAGGCAACACCAGCTGTa > 1:3051525/1‑49 (MQ=255)
tCTTCCGGAATATCCAGCGGCAGGTAGAGCGAAGACAACACCAGCTGTACGGCTTCATCCCACGGGTTAt < 1:3143975/70‑1 (MQ=255)
tCTTCCGGAATATCCAGCGGCAGGTAGAGCGAAGACAACACCAGCTGTACGGCTTCATCCCACGGGTTAt < 1:2186337/70‑1 (MQ=255)
gAATATCCAGCGGCAGGTAGAGCGAAGGCAACACCAGCTGTACgg < 1:2211531/45‑1 (MQ=255)
gAATATCCAGCGGCAGGTAGAGCGAAGGCAACACCAGCTGTACgg < 1:3970257/45‑1 (MQ=255)
gAATATCCAGCGGCAGGTAGAGCGAAGGCAACACCAGCTGTACgg < 1:1388211/45‑1 (MQ=255)
gAATATCCAGCGGCAGGTAGAGCGAAGGCAACACCAGCTGTACgg < 1:3641335/45‑1 (MQ=255)
gAATATCCAGCGGCAGGTAGAGCGAAGGCAACACCAGCTGTACgg < 1:3464573/45‑1 (MQ=255)
gAATATCCAGCGGCAGGTAGAGCGAAGGCAACACCAGCTGTACgg < 1:3288824/45‑1 (MQ=255)
gAATATCCAGCGGCAGGTAGAGCGAAGGCAACACCAGCTGTACgg < 1:103013/45‑1 (MQ=255)
tCCAGCGGCAGGTAGAGCGAAGACAACACCAGCTGTACGGCTTCATCCCACGGGTTATCGGTACCGTGAcc > 1:3970443/1‑71 (MQ=255)
tCCAGCGGCAGGTAGAGCGAAGACAACACCAGCTGTACGGCTTCATCCCACGGGTTATCGGTACCGTGAcc > 1:261908/1‑71 (MQ=255)
tCCAGCGGCAGGTAGAGCGAAGACAACACCAGCTGTACGGCTTCATCCCACGGGTTATCGGTACCGTGAcc > 1:2886513/1‑71 (MQ=255)
ccAGCGGCAGGTAGAGCGAAGACAACACCAGCTGTACGGCTTCATCCCACGGGTTATCGGTACCGTGACCg > 1:3549339/1‑71 (MQ=255)
ccAGCGGCAGGTAGAGCGAAGACAACACCAGCTGTACGGCTTCATCCCACGGGTTATCGGTACCGTGACCg > 1:1149045/1‑71 (MQ=255)
ccAGCGGCAGGTAGAGCGAAGACAACACCAGCTGTACGGCTTCATCCCACGGGTTATCGGTACCGTGACCg > 1:1292494/1‑71 (MQ=255)
ccAGCGGCAGGTAGAGCGAAGACAACACCAGCTGTACGGCTTCATCCCACGGGTTATCGGTACCGTGACCg > 1:345584/1‑71 (MQ=255)
cAGCGGCAGGTAGAGCGAAGGCAACACCAGCTGTACGGCTTCATCCCACGGGTTATCGGTACCGTGACCg > 1:2623683/1‑70 (MQ=255)
cAGCGGCAGGTAGAGCGAAGACAACACCAGCTGTACGGCTTCATCCCACGGGTTATCGGTACCGTGACCGt < 1:1136796/71‑1 (MQ=255)
gCGGCAGGTAGAGCGAAGACAACACCAGCTGTACGGCTTCATCCCACGGGTTATCGGTACCGTGACCGTAc > 1:4142049/1‑71 (MQ=255)
ggCAGTTAGAGCGAAGGCAACACCAGCTGTACGGCTTCATCCCACGGGTTATCGGTACCGTGACCGTACCa < 1:3999745/71‑1 (MQ=255)
ggCAGGTAGAGCGAAGGCAACACCAGCTGTACGGCTTCATCCCACGGGTTATCGGTACCGTGACCGTAc > 1:1526894/1‑69 (MQ=255)
ggCAGGTAGAGCGAAGGCAACACCAGCTGTACGGCTTCATCCCACGGGTTATCGGTACCGTGACCGTAc > 1:3651671/1‑69 (MQ=255)
ggCAGGTAGAGCGAAGGCAACACCAGCTGTACGGCTTCATCCCACGGGTTATCGGTACCGTGACCGTACCa < 1:913603/71‑1 (MQ=255)
ggCAGGTAGAGCGAAGGCAACACCAGCTGTACGGCTTCATCCCACGGGTTATCGGTACCGTGACCGTACCa < 1:884616/71‑1 (MQ=255)
ggCAGGTAGAGCGAAGGCAACACCAGCTGTACGGCTTCATCCCACGGGTTATCGGTACCGTGACCGTACCa < 1:1695278/71‑1 (MQ=255)
ggCAGGTAGAGCGAAGACAACACCAGCTGTACGGCTTCATCCCACGGGTTATCGGTACCGTGACCGTACCa < 1:2552986/71‑1 (MQ=255)
gCAGGTAGAGCGAAGACAACACCAGCTGTACGGCTTCATACCACGGGTTATCGGTACCGTGACCGTAc > 1:2316661/1‑68 (MQ=255)
cAGGTAGAGCGAAGGCAACACCAGCTGTACGGCTTCATCCCACGGGTTATCGGTACCGTGACCGt < 1:1251016/65‑1 (MQ=255)
cAGGTAGAGCGAAGGCAACACCAGCTGTACGGCTTCATCCCACGGGTTATCGGTACCGTGACCGt < 1:1403587/65‑1 (MQ=255)
cAGGTAGAGCGAAGGCAACACCAGCTGTACGGCTTCATCCCACGGGTTATCGGTACCGTGACCGt < 1:4216081/65‑1 (MQ=255)
cAGGTAGAGCGAAGGCAACACCAGCTGTACGGCTTCATCCCACGGGTTATCGGTACCGTGACCGt < 1:3412275/65‑1 (MQ=255)
cAGGTAGAGCGAAGGCAACACCAGCTGTACGGCTTCATCCCACGGGTTATCGGTACCGTGACCGt < 1:933688/65‑1 (MQ=255)
cAGGTAGAGCGAAGGCAACACCAGCTGTACGGCTTCATCCCACGGGTTATCGGTACCGTGACCGTACCAGa < 1:1976979/71‑1 (MQ=255)
aGGTAGAGCGAAGGCAACACCAGCTGTACGGCTTCATCCCACGGGTTATCGGTACCGTGACCGTACCAGa < 1:4081005/70‑1 (MQ=255)
aGGTAGAGCGAAGACAACACCAGCTGTACGGCTTCATCCCACGGGTTATCGGTACCGTGACCGt < 1:966166/64‑1 (MQ=255)
agagCGAAGGCAACACCAGCTGTACGGCTTCATCCCACGGGTTATCGGTACCGTGACCGTACCAGATAttt > 1:2838335/1‑71 (MQ=255)
agagCGAAGGCAACACCAGCTGTACGGCTTCATCCCACGGGTTATCGGTACCGTGACCGTACCAGATAttt > 1:3559460/1‑71 (MQ=255)
agagCGAAGGCAACACCAGCTGTACGGCTTCATCCCACGGGTTATCGGTACCGTGACCGTACCAGATAtt > 1:3579232/1‑70 (MQ=255)
agagCGAAGGCAACACCAGCTGTACGGCTTCATCCCACGGGTTATAGGTACCGTGACCGTACCAGATAttt > 1:2512050/1‑71 (MQ=255)
agagCGAAGACAACACCAGCTGTACGGCTTCATCCCACGGGTTATCGGTACCGTGACCGTACCAGATAttt > 1:2123009/1‑71 (MQ=255)
gagCGAAGACAACACCAGCTGTACGGCTTCATCCCACGGGTTATCGGTACCGTGACCGTACCAGATAttt < 1:1918966/70‑1 (MQ=255)
gCGAAGACAACACCAGCTGTACGGCTTCATCCCACGGGtt > 1:2043189/1‑40 (MQ=255)
gCGAAGACAACACCAGCTGTACGGCTTCATCCCACGGGtt > 1:2665560/1‑40 (MQ=255)
gCGAAGACAACACCAGCTGTACGGCTTCATCCCACGGGtt > 1:2737736/1‑40 (MQ=255)
gCGAAGACAACACCAGCTGTACGGCTTCATCCCACGGGtt > 1:1790525/1‑40 (MQ=255)
gCGAAGACAACACCAGCTGTACGGCTTCATCCCACGGGtt > 1:3265259/1‑40 (MQ=255)
cGAAGACAACACCAGCTGTACGGCTTCATCCCACGGGTTATCGGTACCGTGACCGTACCAGATAttt < 1:2735510/67‑1 (MQ=255)
ggCAACACCAGCTGTACGGCTTCATCCCACGGGTTATCGGTACCGTGCCCGTACCAGa > 1:911969/1‑58 (MQ=255)
ggCAACACCAGCTGTACGGCTTCATCCCACGGGTTATCGGTACCGTGACCGTACCAGa > 1:1649849/1‑58 (MQ=255)
ggCAACACCAGCTGTACGGCTTCATCCCACGGGTTATCGGTACCGTGACCGTACCAGa > 1:1988373/1‑58 (MQ=255)
gCAACACCAGCTGTACGGCTTCATCCCACGGGTTATCGGTACCGTGACCGTACCAGATATTTGCCGCGCTg < 1:810547/71‑1 (MQ=255)
gCAACACCAGCTGTACGGCTTCATCCCACGGGTTATCGGTACCGTGACCGTACCAGATATTTGCCGCGCTg < 1:1101596/71‑1 (MQ=255)
|
TTTTTCGCTGGAGGTCAGACGCGCGGTGCGCATATCTTCCGGAATATCCAGCGGCAGGTAGAGCGAAGGCAACACCAGCTGTACGGCTTCATCCCACGGGTTATCGGTACCGTGACCGTACCAGATATTTGCCGCGCTG > minE/1533474‑1533612
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
