Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I0 R2
|
230 |
93.6 |
4701257 |
96.3% |
4527310 |
61.2 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
244,761 |
C→T |
39.1% |
G221R (GGG→AGG) |
sbcD ← |
exonuclease, dsDNA, ATP‑dependent |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 244,761 | 0 | C | T | 39.1%
| 50.9
/ 71.5
| 69 | G221R (GGG→AGG) | sbcD | exonuclease, dsDNA, ATP‑dependent |
| Reads supporting (aligned to +/- strand): ref base C (18/24); new base T (13/14); total (31/38) |
| Fisher's exact test for biased strand distribution p-value = 8.05e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.05e-01 |
ATGGGGGAGCCGCAATAGCGAACATGTTCCATGCCGCCAATAATCTGTGCGCGGTGAATATGCCCGAGCGCGATGTAGTCGGCTGGTGGAAAGTTTTGTGCCGGAAACGCGTCCAGCGTGCCAATATAAATGT > minE/244697‑244829
|
aTGGGGGAGCCGCAATAGCGAACATGTTCCATGCCGCCAATAATCTGTGCGCGGTGAATATGCCTGAgcgc > 1:3979400/1‑71 (MQ=255)
aTGGGGGAGCCGCAATAGCGAACATGTTCCATGCCGCCAATAATCTGTGCGCGGTGAATATGCCCGAgcgc < 1:3684698/71‑1 (MQ=255)
tGGGGGAGCCGCAATAGCGAACATGTTCCATGCCGCCAATAATCTGTGCGCGGTGAATATGCCCGAgcgc > 1:710494/1‑70 (MQ=255)
tGGGGGAGCCGCAATAGCGAACATGTTCCATGCCGCCAATAATCTGTGCGCGGTGAATATGCCCGAGcgcg < 1:3381322/71‑1 (MQ=255)
gggAGCCGCAATAGCGAACATGTTCCATGCCGCCAATAATCTGTGCGCGGTGAATATGCCCGAGCGCGATg < 1:58207/71‑1 (MQ=255)
gggAGCCGCAATAGCGAACATGTTCCATGCCGCCAATAATCTGTGCGCGGTGAATATGCCCGAGCGCGATg < 1:2196045/71‑1 (MQ=255)
gAGCCGCAATAGCGAACATGTTCCATGCCGCCAATAATCTGTGCGCGGTGAATATGCCCGAGCGCGATGTa < 1:2556003/71‑1 (MQ=255)
gAGCCGCAATAGCGAACATGTTCCATGCCGCCAATAATCTGTGCGCGGTGAATATGCCCGAGCGCGATGTa < 1:3583324/71‑1 (MQ=255)
aGCCGCAATAGCGAACATGTTCCATGCCGCCAATAATCTGTGCGCGGTGAATATGCCTGAGCGCGATGTAg > 1:320207/1‑71 (MQ=255)
gCCGCAATAGCGAACATGTTCCATGTCGCCAATAATCTGTGCGCGGTGAATATGCCCGAGCGCGATGTAGt > 1:4237853/1‑71 (MQ=255)
gCAATAGCGAACATGTTCCATGCCGCCAATAATCTGTGCGCGGTGAATATGCCTGAGCGCGATGTAGTCgg > 1:1912817/1‑71 (MQ=255)
aaTAGCGAACATGTTCCATGCCGCCAATAATCTGTGCGCGGTGAATATGCCTGAGCGCGATGTAGTCGGCt > 1:4232721/1‑71 (MQ=255)
aGCGAACATGTTCCATGCCGCCATTAATCTGTGCGCGGTGAATATGCCCGAGCGCGATGTAGTCGGCtggt > 1:4614247/1‑71 (MQ=255)
aGCGAACATGTTCCATGCCGCCAATAATCTGTGCGCGGTGAATATGCCCGAGCGCGATGTAGTCGGCtggt > 1:4608749/1‑71 (MQ=255)
gCGAACATGTTCCATGCCGCCAATAATCTGTGCGCGGTGAATATGCCCGAGCGCGATGTAg > 1:1621678/1‑61 (MQ=255)
gCGAACATGTTCCATGCCGCCAATAATCTGTGCGCGGTGAATATGCCCGAGCGCGATGTAg > 1:2118105/1‑61 (MQ=255)
cATGTTCCATGCCGCCAATAATCTGTGCGCGGTTAATATGCCCGAGCGCGATGTAGTCGGCTGGTGGAAAg < 1:2305450/71‑1 (MQ=255)
cATGTTCCATGCCGCCAATAATCTGTGCGCGGTGAATATGCCCGAGCGCGATGTAGTCGGCTGGTGGAAAg < 1:4578208/71‑1 (MQ=255)
cATGTTCCATGCCGCCAATAATCTGTGCGCGGTGAATATGCCCGAGCGCGATGTAGTCGGCTGGTGGAAAg > 1:3057552/1‑71 (MQ=255)
tGTTCCATGCCGCCAATAATCTGTGCGCGGTGAATATGCCTGAGCGCGATGTAGTCGGCTGGTGGAAAGtt < 1:1636096/71‑1 (MQ=255)
tGTTCCATGCCGCCAATAATCTGTGCGCGGTGAATATGCCTGAGCGCGATGTAGTCGGCTGGTGGAAAGt < 1:3763664/70‑1 (MQ=255)
tGTTCCATGCCGCCAATAATCTGTGCGCGGTGAATATGCCCGAGCGCGATGTAg < 1:2507124/54‑1 (MQ=255)
tGTTCCATGCCGCCAATAATCTGTGCGCGGTGAATATGCCCGAGCGCGATGTAGTCGGCTGGTGGAAAGtt < 1:955570/71‑1 (MQ=255)
tGTTCCATGCCGCCAATAATCTGTGCGCGGTGAATATGCCCGAGCGCGATGTAGTCGGCTGGTGGAAAGtt < 1:2049821/71‑1 (MQ=255)
gTTCCATGCCGCCAATAATCTGTGCGCGGTGAATATGCCCGAGCGCGATGTAGTCGGCtggtg > 1:270619/1‑63 (MQ=255)
gTTCCATGCCGCCAATAATCTGTGCGCGGTGAATATGCCCGAGCGCGATGTAGTCGGCTGGTGGAAAGttt > 1:276112/1‑71 (MQ=255)
ttCCATGCCGCCAATAATCTGTGCGCGGTGAATATGCCTGAGCGCGATGTAGTCGGCTGGTGGAAAGttt < 1:4276158/70‑1 (MQ=255)
ttCCATGCCGCCAATAATCTGTGCGCGGTGAATATGCCCGAGCGCGATg < 1:4287465/49‑1 (MQ=255)
ttCCATGCCGCCAATAATCTGTGCGCGGTGAATATGCCCGAGCGCGATGTAg < 1:2116602/52‑1 (MQ=255)
cATGCCGCCAATAATCTGTGCGCGGTGAATATGCCTGAGCGCGATGTAGTCGGCTGGTGGAAAGTTTtgt < 1:3063905/70‑1 (MQ=255)
aTGCCGCCAATAATCTGTGCGCGGTGAATATGCCTGAGCGCGATGTAGTCGGCTGGTGGAAAGTTTTGTGc > 1:1377725/1‑71 (MQ=255)
tGCCGCCAATAATCTGTGCGCGGTGAATATGCCCGAGCGCGATg < 1:1139409/44‑1 (MQ=255)
gccgccAATAATCTGTGCGCGGTGAATATGCCCGAGCGCGATg < 1:1133858/43‑1 (MQ=255)
ccgccAATAATCTGTGCGCGGTGAATATGCCTGAGCGCGATGTAGTCGGCTGGTGGAAAGTTTTGTGcc > 1:2350170/1‑69 (MQ=255)
ccgccAATAATCTGTGCGCGGTGAATATGCCTGAGCGCGATGTAGTCGGCTGGTGGAAAGTTTTGTGCCgg < 1:3996868/71‑1 (MQ=255)
gccAATAATCTGTGCGCGGTGAATATGCCCGAGCGCGATGTAGTCGGCTGGTGGAAAGTTTTGTGCCGGa > 1:1493106/1‑70 (MQ=255)
cAATAATCTGTGCGCGGTGAATATGCCTGAGcgcg < 1:3229520/35‑1 (MQ=255)
cAATAATCTGTGCGCGGTGAATATGCCTGAGCGCGATGTAGTCGGCTGGTGGAAAGTTTTGTGCCGGAAAc < 1:3507617/71‑1 (MQ=255)
aataatCTGTGCGCGGTGAATATGCCCGAGCGCGATGTAGTCGGCTGGTGGAAAGTTTtgtg > 1:2271962/1‑62 (MQ=255)
taatCTGTGCGCGGTGAATATGCCTGAGCGCGATGTAGTCGGCTGGTGGaa < 1:3514358/51‑1 (MQ=255)
taatCTGTGCGCGGTGAATATGCCCGAGCGCGATGTAGTCGGCTGGTGGAAAGTTTTGTGCCGGAAAcgcg < 1:3651241/71‑1 (MQ=255)
aatCTGTGCGCGGTGAATATGCCTGAGCGCGATGTAGTCGGCTGGTGGAAAGtt > 1:1424923/1‑54 (MQ=255)
atCTGTGCGCGGTGAATATGCCCGAGCGCGATGTAGTCGGCtg > 1:4497600/1‑43 (MQ=255)
tgCGCGGTGAATATGCCTGAGCGCGATGTAGTCGGCTGGTGGAAAGTTTTGTGCCGGAAACGCGTCCAg < 1:3448480/69‑1 (MQ=255)
tgCGCGGTGAATATGCCCGAGCGCGATGTAGTCGGCTGGTGGAAAGTTTTGTGCCGGAAACGCGTCCAGCg > 1:375101/1‑71 (MQ=255)
tgCGCGGTGAATATGCCCGAGCGCGATGTAGTCGGCTGGTGGAAAGTTTTGTGCCGGAAACGCGTCCAGCg < 1:3077347/71‑1 (MQ=255)
gcgcGGTGAATATGCCTGAGCGCGATGTAGTCGGCTGGTGGAAAGttt < 1:2037544/48‑1 (MQ=255)
cgcgGTGAATATGCCTGAGCGCGATGTAGTCGGCTGGTGGAAAGTTTTGTGCCGGAAACGCGTCCAGCGTg > 1:4487220/1‑71 (MQ=255)
gcgGTGAATATGCCCGAGCGCGATGTGGTCGGCTGGTGGAAAGTTTTGTGCCGGAAACGCGTCCAGCGTGc < 1:2855936/71‑1 (MQ=255)
gcgGTGAATATGCCCGAGCGCGATGTAGTCGGCTGGTGGAAAGTTTTGTGCCGGAAACGCGTCCAGCGTGc > 1:2505367/1‑71 (MQ=255)
cgGTGAATATGCCCGAGCGCGATGTAGTCGGCTGGTGGAAAGTTTTGTGCCGGAAACGCGTCCAGCGTGc > 1:4372218/1‑70 (MQ=255)
cgGTGAATATGCCCGAGCGCGATGTAATCGGCTGGTGGAAAGTTTTGTGCCGGAAACGCGTCCAGCGTGcc < 1:1695029/71‑1 (MQ=255)
tGAATATGCCTGAGCGCGATGTAGTCGGCTGGTGGAAAGTTTTGTGCCGGAAACGCGTCCAGCGTGCCaa > 1:478308/1‑70 (MQ=255)
tGAATATGCCCGAGCGCGATGTAGTCGGCTGGTGGAAAGtt < 1:2906398/41‑1 (MQ=255)
aaTATGCCTGAGCGCGATGTAGTCGGCTGGTGGAAAGTTTTGTGCCGGAAACGCGTCCAGCGTGCCAatat < 1:2788139/71‑1 (MQ=255)
aaTATGCCCGAGCGCGATGTAGTCGGCTGGTGGAAAGtttt > 1:4235935/1‑41 (MQ=255)
atatGCCTGAGCGCGATGTAGTCGGCTGGTGGAAAGTTTTGTGCCGGAAACGCGTCCAGCGTGCCAatat > 1:3660931/1‑70 (MQ=255)
atatGCCTGAGCGCGATGTAGTCGGCTGGTGGAAAGTTTTGTGCCGGAAACGCGTCCAGCGTGCCAatat > 1:85986/1‑70 (MQ=255)
atatGCCTGAGCGCGATGTAGTCGGCTGGTGGAAAGTTTTGTGCCGGAAACGCGTCCAGCGTGCCAAtata < 1:4157659/71‑1 (MQ=255)
atatGCCTGAGCGCGATGTAGTCGGCTGGTGGAAAGTTTTGTGCCGGAAACGCGTCCAGCGTGCCAAtata < 1:2197829/71‑1 (MQ=255)
atatGCCTGAGCGCGATGTAGTCGGCTGGTGGAAAGTTTTGTGCCGGAAACGCGTCCAGCGTGCCAAtata < 1:537717/71‑1 (MQ=255)
atatGCCCGAGCGCGATGTAGTCGGCTGGTGGAAAGTTTTGTGCCGGAAACGCGTCCAGCGTGCCAatat > 1:161868/1‑70 (MQ=255)
tatGCCCGAGCGCGATGTAGTCGGCTGGTGGAAAGTTTTGTGc > 1:2128687/1‑43 (MQ=255)
tatGCCCGAGCGCGATGTAGTCGGCTGGTGGAAAGTTTTGTGCCGGAAACGCGTCCAGCGTGCCa < 1:565045/65‑1 (MQ=255)
tatGCCCGAGCGCGATGTAGTCGGCTGGTGGAAAGTTTTGTGCCGGAAACGCGTCCAGCGTGCCAAtata < 1:941429/70‑1 (MQ=255)
tatGCCCGAGCGCGATGTAGTCGGCTGGTGGAAAGTTTTGTGCCGGAAACGCGTCCAGCGTGCCAATATaa < 1:166549/71‑1 (MQ=255)
tGCCCGAGCGCGATGTAGTCGGCTGGTGGAAAGTTTTGTGCCGGAAACGCGTCCAGCGTGCCAAtata < 1:4423294/68‑1 (MQ=255)
gCCTGAGCGCGATGTAGTCGGCTGGTGGAAAGTTTTGTGCCGGAAACGCGTCCAGCGTGCCAATATaa > 1:3841255/1‑68 (MQ=255)
gCCTGAGCGCGATGTAGTCGGCTGGTGGAAAGTTTTGTGCCGGAAACGCGTCCAGCGTGCCAATATaa > 1:546742/1‑68 (MQ=255)
cccGAGCGCGATGTAGTCGGCTGGTGGAAAGTTTTGTGCCGGAAACGCGTCCAGCGTGCCAATATAAATg < 1:3875163/70‑1 (MQ=255)
cccGAGCGCGATGTAGTCGGCTGGTGGAAAGTTTTGTGCCGGAAACGCGTCCAGCGTGCCAATATAAATGt > 1:329979/1‑71 (MQ=255)
ccGAGCGCGATGTAGTCGGCTGGTGGAAAGTTTTGTGcc > 1:1801636/1‑39 (MQ=255)
cGAGCGCGATGTAGTCGGCTGGTGGAAAGTTTTGTGCCGGa < 1:2650914/41‑1 (MQ=255)
cGAGCGCGATGTAGTCGGCTGGTGGAAAGTTTTGTGCCGGAAACGCGTCCAGCGTGCCa < 1:1424634/59‑1 (MQ=255)
cGAGCGCGATGTAGTCGGCTGGTGGAAAGTTTTGTGCCGGAAACGCGTCCAGCGTGCCa < 1:1111497/59‑1 (MQ=255)
|
ATGGGGGAGCCGCAATAGCGAACATGTTCCATGCCGCCAATAATCTGTGCGCGGTGAATATGCCCGAGCGCGATGTAGTCGGCTGGTGGAAAGTTTTGTGCCGGAAACGCGTCCAGCGTGCCAATATAAATGT > minE/244697‑244829
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
