Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F21 I0 R2
|
308 |
27.9 |
1975068 |
96.7% |
1909890 |
67.2 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
W3110S.gb |
904,996 |
G→A |
100% |
intergenic (‑107/‑19) |
ybjP ← / → ybjQ |
predicted lipoprotein/conserved hypothetical protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 904,996 | 0 | G | A | 100.0%
| 102.2
/ NA
| 32 | intergenic (‑107/‑19) | ybjP/ybjQ | predicted lipoprotein/conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (8/24); total (8/24) |
| Rejected as polymorphism: Frequency below/above cutoff threshold. |
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. |
ATGATATCCTTTCAATAATAGCGTATCAGTCTGATAATGCTTTTGAGATCGAAGGCTTAGCAAACAAGGAGATCGATCATGCAATTTTCTACAACCCCAACTCTGGAAGGCCAGACCATCGTTGAA > W3110S.gb/904937‑905062
|
atGATATCCTTTCAATAATAGCGTATCAGTCTGATAATGCTTTTGAGATCGAAGGCTTAACAAACAAGga < 1:1700691/70‑1 (MQ=255)
atGATATCCTTTCAATAATAGCGTATCAGTCTGATAATGCTTTTGAGATCGAAGGCTTAACAAACAAGga < 1:1952963/70‑1 (MQ=255)
atGATATCCTTTCAATAATAGCGTATCAGTCTGATAATGCTTTTGAGATCGAAGGCTTAACAAACAAGga < 1:1476981/70‑1 (MQ=255)
atGATATCCCTTCAATAATAGCGTATCAGTCTGATAATGCTTTTGAGATCGAAGGCTTAACAAACAAGga < 1:931435/70‑1 (MQ=255)
tGATATCCTTTCAATAATAGCGTATCAGTCTGATAATGCTTTTGAGATCGAAGGCTTAACAAACAAGga < 1:160388/69‑1 (MQ=255)
tGATATCCTTTCAATAATAGCGTATCAGTCTGATAATGCTTTTGAGATCGAAGGCTTAACAAACAAGga < 1:451892/69‑1 (MQ=255)
gATATCCTTTCAATAATAGCGTATCAGTCTGATAATGCTTTTGAGATCGAAGGCTTAACAAACAAg > 1:1041978/1‑66 (MQ=255)
gATATCCTTTCAATAATAGCGTATCAGTCTGATAATGCTTTTGAGATCGAAGGCTTAACAAACAAg > 1:1962774/1‑66 (MQ=255)
gATATCCTTTCAATAATAGCGTATCAGTCTGATAATGCTTTTGAGATCGAAGGCTTAACAAACAAg > 1:284363/1‑66 (MQ=255)
gATATCCTTTCAATAATAGCGTATCAGTCTGATAATGCTTTTGAGATCGAAGGCTTAACAAACAAg > 1:871150/1‑66 (MQ=255)
tCCTTTCAATAATAGCGTATCAGTCTGATAATGCTTTTGAGATCGAAGGCTTAACAAACAAGGAgatcgat < 1:1368163/71‑1 (MQ=255)
tCCTTTCAATAATAGCGTATCAGTCTGATAATGCTTTTGAGATCGAAGGCTTAACAAACAAGGAgatcgat < 1:700910/71‑1 (MQ=255)
tCCTTTCAATAATAGCGTATCAGTCTGATAATGCTTTTGAGATCGAAGGCTTAACAAACAAGGAgatcgat < 1:1875689/71‑1 (MQ=255)
tCCTTTCAATAATAGCGTATCAGTCTGATAATGCTTTTGAGATCGAAGGCTTAACAAACAAGGAgatcgat < 1:1768569/71‑1 (MQ=255)
aTAATGCTTTTGAGATCGAAGGCTTAACAAACAAGGAGATCGATCATGCAATTTTCTACAACCCCAAc < 1:49302/68‑1 (MQ=255)
aTAATGCTTTTGAGATCGAAGGCTTAACAAACAAGGAGATCGATCATGCAATTTTCTACAACCCCAAc < 1:919911/68‑1 (MQ=255)
aTAATGCTTTTGAGATCGAAGGCTTAACAAACAAGGAGATCGATCATGCAATTTTCTACAACCCCAAc < 1:1753114/68‑1 (MQ=255)
aTAATGCTTTTGAGATCGAAGGCTTAACAAACAAGGAGATCGATCATGCAATTTTCTACAACCCCAAc < 1:1793968/68‑1 (MQ=255)
aTAATGCTTTTGAGATCGAAGGCTTAACAAACAAGGAGATCGATCATGCAATTTTCTACAACCCCAAc < 1:1625693/68‑1 (MQ=255)
aTGCTTTTGAGATCGAAGGCTTAACAAACAAGGAGATCGATCATGCAATTTTCTACAACCCCAACTCTgg > 1:1326713/1‑70 (MQ=255)
aTGCTTTTGAGATCGAAGGCTTAACAAACAAGGAGATCGATCATGCAATTTTCTACAACCCCAACTCTgg > 1:873109/1‑70 (MQ=255)
aTGCTTTTGAGATCGAAGGCTTAACAAACAAGGAGATCGATCATGCAATTTTCTACAACCCCAACTCTgg > 1:1158847/1‑70 (MQ=255)
aTGCTTTTGAGATCGAAGGCTTAACAAACAAGGAGATCGATCATGCAATTTTCTACAACCCCAACTCTgg > 1:1111184/1‑70 (MQ=255)
cTTAACAAACAAGGAGATCGATCATGCAATTTTCTACAACCCCAACTCTGGAAGGCCAGACCATCGTTGaa < 1:1000041/71‑1 (MQ=255)
cTTAACAAACAAGGAGATCGATCATGCAATTTTCTACAACCCCAACTCTGGAAGGCCAGACCATCGTTGaa < 1:1702051/71‑1 (MQ=255)
cTTAACAAACAAGGAGATCGATCATGCAATTTTCTACAACCCCAACTCTGGAAGGCCAGACCATCGTTGaa < 1:1688374/71‑1 (MQ=255)
cTTAACAAACAAGGAGATCGATCATGCAATTTTCTACAACCCCAACTCTGGAAGGCCAGACCATCGTTGaa < 1:1969344/71‑1 (MQ=255)
cTTAACAAACAAGGAGATCGATCATGCAATTTTCTACAACCCCAACTCTGGAAGGCCAGACCATCGTTGaa < 1:1526562/71‑1 (MQ=255)
cTTAACAAACAAGGAGATCGATCATGCAATTTTCTACAACCCCAACTCTGGAAGGCCAGACCATCGTTGaa < 1:1488800/71‑1 (MQ=255)
cTTAACAAACAAGGAGATCGATCATGCAATTTTCTACAACCCCAACTCTGGAAGGCCAGACCATCGTTGaa < 1:1414828/71‑1 (MQ=255)
cTTAACAAACAAGGAGATCGATCATGCAATTTTCTACAACCCCAACTCTGGAAGGCCAGACCATCGTTGaa < 1:1367573/71‑1 (MQ=255)
cTTAACAAACAAGGAGATCGATCATGCAATTTTCTACAACCCCAACTCTGGAAGGCCAGACCATCGTTGaa < 1:997862/71‑1 (MQ=255)
|
ATGATATCCTTTCAATAATAGCGTATCAGTCTGATAATGCTTTTGAGATCGAAGGCTTAGCAAACAAGGAGATCGATCATGCAATTTTCTACAACCCCAACTCTGGAAGGCCAGACCATCGTTGAA > W3110S.gb/904937‑905062
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
