Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F8 I0 R1
|
311 |
50.1 |
4085234 |
95.9% |
3917739 |
60.7 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
W3110S.gb |
905,152 |
Δ1 bp |
10.9% |
coding (138/324 nt) |
ybjQ → |
conserved hypothetical protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 905,152 | 0 | T | . | 10.9%
| 114.1
/ 11.9
| 46 | coding (138/324 nt) | ybjQ | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base T (20/21); new base . (3/2); total (23/23) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
GCGAAGCGATTTTAGGTGCCAATATTTTCCGTGATTTCTTTGCCGGTATCCGCGATATCGTTGGCGGACGTTCCGGTGCGTATGAAAAAGAACTGCGTAAAGCACGGGAGATCGCCTTTGAGGAA > W3110S.gb/905082‑905206
|
gCGAAGCGATTTTAGGTGCCAATATTTTCCGTGATTTCTTTGCCGGTATCCGCGATATCGTTGGCGGACGt > 1:3431039/1‑71 (MQ=255)
aaGCGATTTTAGGTGCCAATATTTTCCGTGATTTCTTTGCCGGTATCCGCGATATCGTTGGCGGACGTTcc < 1:168129/71‑1 (MQ=255)
ttttAGGTGCCAATATTTTCCGTGATTTCTTTGCCGGTATCCGCGATATCGTTGGCGGACGTTCCGGTGCg < 1:2879723/71‑1 (MQ=255)
ttttAGGTGCCAATATTTTCCGTGATTTCTTTGCCGGTATCCGCGATATCGTTGGCGGACGTTCCGGTGCg < 1:1421223/71‑1 (MQ=255)
ttttAGGTGCCAATATTTTCCGTGATTTCTTTGCCGGTATCCGCGATATCGTTGGCGGACGTTCCGGTGCg < 1:3710712/71‑1 (MQ=255)
tttAGGTGCCAATATTTTCCGTGATTTCTTTGCCGGTATCCGCGATATCGTTGGCGGACGTTCCGGTGCg > 1:1488688/1‑70 (MQ=255)
tttAGGTGCCAATATTTTCCGTGATTTCTTTGCCGGTATCCGCGATATCGTTGGCGGACGTTCCGGTGCg > 1:3799902/1‑70 (MQ=255)
tttAGGTGCCAATATTTTCCGTGATTTCTTTGCCGGTATCCGCGATATCGTTGGCGGACGTTCCGGTGCg < 1:683291/70‑1 (MQ=255)
tttAGGTGCCAATATTTTCCGTGATTTCTTTCCCGGTATCCGCGATATCGTTGGCGGACGTTCCGGTGCg > 1:3527298/1‑70 (MQ=255)
ggTGCCAATATTTTCCGTGATTTCTTTGCCGGTATCCGCGATATCGTTGGCGGACGTTCCGGTGCGTATg < 1:3908055/70‑1 (MQ=255)
ggTGCCAATATTTTCCGTGATTTCTTTGCCGGTATCCGCGATATCGTTGGCGGACGTTCCGGTGCGTATGa < 1:1238570/71‑1 (MQ=255)
gCCAATATTTTCCGTGATTTCTTTGCCGGTATCCGCGATATCGTTGGCGGACG‑TCCGGTGCGTa > 1:2667592/1‑64 (MQ=255)
gCCAATATTTTCCGTGATTTCTTTGCCGGTATCCGCGATATCGTTGGCGGACG‑TCCGGTGCGTa > 1:281983/1‑64 (MQ=255)
gCCAATATTTTCCGTGATTTCTTTGCCGGTATCCGCGATATCGTTGGCGGACG‑TCCGGTGCGTa > 1:695164/1‑64 (MQ=255)
tatTTTCCGTGATTTCTTTGCCGGTATCCGCGATATCGTTGGCGGACGTTcc < 1:3793189/52‑1 (MQ=255)
tatTTTCCGTGATTTCTTTGCCGGTATCCGCGATATCGTTGGCGGACGTTcc < 1:1341507/52‑1 (MQ=255)
atTTTCCGTGATTTCTTTGCCGGTATCCGCGATATCGTTGGCGGACGtt > 1:572034/1‑49 (MQ=255)
atTTTCCGTGATTTCTTTGCCGGTATCCGCGATATCGTTGGAGGACGtt > 1:3337325/1‑49 (MQ=255)
ttttCCGTGATTTCTTTGCCGGTATCCGCGATATCGTTGGCGGACGTTCCGGTGCGTATGAAAAAGAACTg > 1:3034553/1‑71 (MQ=255)
ttttCCGTGATTTCTTTGCCGGTATCCGCGATATCGTTGGCGGACGTTCCGGTGCGTATGAAAAAGAACTg > 1:485761/1‑71 (MQ=255)
ttttCCGTGATTTCTTTGCCGGTATCCGCGATATCGTTGGCGGACGTTCCGGTGCGTATGAAAAAGAACTg > 1:2857674/1‑71 (MQ=255)
cGTGATTTCTTTGCCGGTATCCGCGATATCGTTGGCGGACGTTCCGGTGCGTATGAAAAAGAACTGCGTa < 1:3459039/70‑1 (MQ=255)
cGTGATTTCTTTGCCGGTATCCGCGATATCGTTGGCGGACGTTCCGGTGCGTATGAAAAAGAACTGCGTa < 1:1693055/70‑1 (MQ=255)
aTTTCTTTGCCGGTATCCGCGATATCGTTGGCGGACGTTCCGGTGCGTATGAAAAAg < 1:151622/57‑1 (MQ=255)
cTTTGCCGGTATCCGCGATATCGTTGGCGGACGTTCCGGTGCGTATGAAAAAGAACTGCGTAAAGCACgg < 1:1779480/70‑1 (MQ=255)
ccGGTATCCGCGATATCGTTGGCGGACGTTCCGGTGCGTATGAAAAAGAACTGCGTAAAGCACGGgag < 1:2940660/68‑1 (MQ=255)
ggTATCCGCGATATCGTTGGCGGACGTTCCGGTGCGTATGAAAAAGAACTGCGTAAAGCAc < 1:3627414/61‑1 (MQ=255)
ggTATCCGAGATATCGTTGGCGGACG‑TCCGGTGCGTATGAAAAAg < 1:2259880/45‑1 (MQ=38)
tCCGCGATATCGTTGGCGGACGTTCCGGTGCGTATGAAAAAg < 1:3309324/42‑1 (MQ=255)
tCCGCGATATCGTTGGCGGACGTTCCGGTGCGTATGAAAAAGAACTGCGTAAAGCACGGgag < 1:3640317/62‑1 (MQ=255)
tCCGCGATATCGTTGGCGGACGTTCCGGTGCGTATGAAAAAGAACTGCGTAAAGCACGGgag < 1:2097124/62‑1 (MQ=255)
tCCGCGATATCGTTGGCGGACGTTCCGGTGCGTATGAAAAAGAACTGCGTAAAGCACGGGAGATCGCCtt < 1:3867008/70‑1 (MQ=255)
cgcgATATCGTTGGCGGACGTTCCTGTGCGTATGAAAAAGAACTGCGTAAAGCACGGGAGATCGCCttt > 1:564233/1‑69 (MQ=255)
cgcgATATCGTTGGCGGACGTTCCGGTGCGTATGAAAAAGAACTGCGTAAAGCACGGGAGAt > 1:2048707/1‑62 (MQ=255)
cgcgATATCGTTGGCGGACGTTCCGGTGCGTATGAAAAAGAACTGCGTAAAGCACGGGAGAt > 1:3644304/1‑62 (MQ=255)
cgcgATATCGTTGGCGGACGTTCCGGTGCGTATGAAAAAGAACTGCGTAAAGCACGGGAGATCGCCttt > 1:3164587/1‑69 (MQ=255)
cgcgATATCGTTGGCGGACGTTCCGGTGCGTATGAAAAAGAACTGCGTAAAGCACGGGAGATCGCCttt > 1:2968876/1‑69 (MQ=255)
cgcgATATCGTTGGCGGACGTTCCGGTGCGTATGAAAAAGAACTGCGTAAAGCACGGGAGATCGCCttt > 1:3554048/1‑69 (MQ=255)
cgcgATATCGTTGGCGGACGTTCCGGTGCGTATGAAAAAGAACTGCGTAAAGCACGGGAGATCGCCttt > 1:1488524/1‑69 (MQ=255)
cgcgATATCGTTGGCGGACGTTCCGGTGCGTATGAAAAAGAACTGCGTAAAGCACGGGAGATCGCCttt > 1:2382120/1‑69 (MQ=255)
cgcgATATCGTTGGCGGACCTTCCGGTGCGTATGAAAAAGAACTGCGTAAAGCACGGgaga > 1:2316229/1‑61 (MQ=255)
gATATCGTTGGCGGACGTTCCGGTGCGTATGAAAAAGAACTGCGTAAAGCACGGGAGATCGCCTTTGAgg < 1:1621506/70‑1 (MQ=255)
atatCGTTGGCGGACG‑TCCGGTGCGTATGAAAAAg < 1:166459/35‑1 (MQ=37)
tCGTTGGCGGACGTTCCGGTGCGTATGAAAAAGAACTGCGTAAAGCACGGgag < 1:162062/53‑1 (MQ=255)
tCGTAGGCGGACGTTCCGGTGCGTATGAAAAAGaa > 1:2947988/1‑35 (MQ=255)
gTTGGCGGCCGTTCCGGTGCGTATGAAAAAGAACTGCGTAAAGCACGGGAGATCGCCTTTGAGGaa > 1:2179410/1‑66 (MQ=255)
gTTGGCGGACGTTCCGGTGCGTATGAAAAAGAACTGCGTaaa > 1:1025370/1‑42 (MQ=255)
gTTGGCGGACGTTCCGGTGCGTATGAAAAAGAACTGCGTAAAGCACGGGAGATCGCCTTTGAGGaa > 1:1060524/1‑66 (MQ=255)
gTTGGCGGACGTTCCGGTGCGTATGAAAAAGAACTGCGTAAAGCACGGGAGATCGCCTTTGAGGaa > 1:1718409/1‑66 (MQ=255)
ttCCGGTGCGTATGAAAAAGAACTGCGTAAAGCACGGGAGATCGCCTTTGAgg < 1:316204/53‑1 (MQ=255)
ttCCGGTGCGTATGAAAAAGAACTGCGTAAAGCACGGGAGATCGCCTTTGAgg < 1:2892888/53‑1 (MQ=255)
ttCCGGTGCGTATGAAAAAGAACTGCGTAAAGCACGGGAGATCGCCTTTGAgg < 1:1582091/53‑1 (MQ=255)
|
GCGAAGCGATTTTAGGTGCCAATATTTTCCGTGATTTCTTTGCCGGTATCCGCGATATCGTTGGCGGACGTTCCGGTGCGTATGAAAAAGAACTGCGTAAAGCACGGGAGATCGCCTTTGAGGAA > W3110S.gb/905082‑905206
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
