Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F28 I2 R2
|
356 |
55.1 |
4139924 |
95.4% |
3949487 |
63.8 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
904,996 |
G→A |
intergenic (‑107/‑19) |
ybjP ← / → ybjQ |
predicted lipoprotein/conserved hypothetical protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 904,996 | 0 | G | A | 95.1%
| 119.2
/ ‑1.2
| 41 | intergenic (‑107/‑19) | ybjP/ybjQ | predicted lipoprotein/conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base G (0/2); new base A (16/23); total (16/25) |
| Fisher's exact test for biased strand distribution p-value = 5.12e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.61e-01 |
TAATAATGATATCCTTTCAATAATAGCGTATCAGTCTGATAATGCTTTTGAGATCGAAGGCTTAGCAAACAAGGAGATCGATCATGCAATTTTCTACAACCCCAACTCTGGAAGGCCAGACCATCGTTGAA > W3110S.gb/904932‑905062
|
taataaTGATATCCTTTCAATAATAGCGTATCAGTCTGATAATGCTTTTGAGATCGAAGGCTTaacaaaca < 1:3882725/71‑1 (MQ=255)
taataaTGATATCCTTTCAATAATAGCGTATCAGTCTGATAATGCTTTTGAGATCGAAGGCTTaacaaaca < 1:3877970/71‑1 (MQ=255)
taataaTGATATCCTTTCAATAATAGCGTATCAGTCTGATAATGCTTTTGAGATCGAAGGCTTaacaaaca < 1:1704863/71‑1 (MQ=255)
taataaTGATATCCTTTCAATAATAGCGTATCAGTCTGATAATGCTTTTGAGATCGAAGGCTTaacaaaca < 1:346203/71‑1 (MQ=255)
taataaTGATATCCTTTCAATAATAGCGTATCAGTCTGATAATGCTTTTGAGATCGAAGGCTTaacaaaca < 1:2886764/71‑1 (MQ=255)
taataaTGATATCCTTTCAATAATAGCGTATCAGTCTGATAATGCTTTTGAGATCGAAGGCTTaacaaaca < 1:1982178/71‑1 (MQ=255)
taataaTGATATCCTTTCAATAATAGCGTATCAGTCTGATAATGCTTTTGAGATCGAAGGCTTaacaaaca < 1:2307828/71‑1 (MQ=255)
aataatGATATCCTTTCAATAATAGCGTATCAGTCTGATAATGCTTTTGAGATCGAAGGCTTaacaaaca < 1:2229329/70‑1 (MQ=255)
tatCCTTTCAATAATAGCGTATCAGTCTGATAATGCTTTTGAGATCGAAGGCTTAACAAACAAGGAgatcg < 1:347231/71‑1 (MQ=255)
tatCCTTTCAATAATAGCGTATCAGTCTGATAATGCTTTTGAGATCGAAGGCTTAACAAACAAGGAgatcg < 1:97870/71‑1 (MQ=255)
tatCCTTTCAATAATAGCGTATCAGTCTGATAATGCTTTTGAGATCGAAGGCTTAACAAACAAGGAgatcg < 1:849182/71‑1 (MQ=255)
tatCCTTTCAATAATAGCGTATCAGTCTGATAATGCTTTTGAGATCGAAGGCTTAACAAACAAGGAgatcg < 1:4092695/71‑1 (MQ=255)
tatCCTTTCAATAATAGCGTATCAGTCTGATAATGCTTTTGAGATCGAAGGCTTAACAAACAAGGAgatcg < 1:3693982/71‑1 (MQ=255)
tatCCTTTCAATAATAGCGTATCAGTCTGATAATGCTTTTGAGATCGAAGGCTTAACAAACAAGGAgatcg < 1:2447549/71‑1 (MQ=255)
atCCTTTCAATAATAGCGTATCAGTCTGATAATGCTTTTGAGATCGAAGGCTTAACa < 1:1338094/57‑1 (MQ=255)
atCCTTTCAATAATAGCGTATCAGTCTGATAATGCTTTTGAGATCGAAGGCTTAACa < 1:780072/57‑1 (MQ=255)
atCCTTTCAATAATAGCGTATCAGTCTGATAATGCTTTTGAGATCGAAGGCTTAACa < 1:625045/57‑1 (MQ=255)
atCCTTTCAATAATAGCGTATCAGTCTGATAATGCTTTTGAGATCGAAGGCTTAACa < 1:787502/57‑1 (MQ=255)
atCCTTTCAATAATAGCGTATCAGTCTGATAATGCTTTTGAGATCGAAGGCTTAACa < 1:3639222/57‑1 (MQ=255)
atCCTTTCAATAATAGCGTATCAGTCTGATAATGCTTTTGAGATCGAAGGCTTAACa < 1:1905605/57‑1 (MQ=255)
atCCTTTCAATAATAGCGTATCAGTCTGATAATGCTTTTGAGATCGAAGGCTTAACa < 1:2012626/57‑1 (MQ=255)
atCCTTTCAATAATAGCGTATCAGTCTGATAATGCTTTTGAGATCGAAGGCTTAACAAACAAGGAgatcg < 1:595265/70‑1 (MQ=255)
atCCTTTCAATAATAGCGTATCAGTCTGATAATGCTTTTGAGATCGAAGGCTTAACAAACAAGGAgatcg < 1:971314/70‑1 (MQ=255)
tCCTTTCAATAATAGCGTATCAGTCTGATAATGCTTTTGAGATCGAAGGCTTAACa < 1:814526/56‑1 (MQ=255)
cGTATCAGTCTGATAATGCTTTTGAGATCGAAGGCTTAACAAACAAGGAGATCGATCATGCAATTTTCTAc < 1:50849/71‑1 (MQ=255)
aGTGTGATAATGCTTTTGAGATCGAAGGCTTAACAAACAAGGAGATCGATCATACAATTTTCTACAAccc > 1:3898631/1‑70 (MQ=255)
aGTCTGATAATGCTTTTGAGATCGAAGGCTTAACAAACAAGGAGATCGATCATACAATTTTCTACAAccc > 1:209417/1‑70 (MQ=255)
aGTCTGATAATGCTTTTGAGATCGAAGGCTTAACAAACAAGGAGATCGATCATACAATTTTCTACAAccc > 1:2382143/1‑70 (MQ=255)
aGTCTGATAATGCTTTTGAGATCGAAGGCTTAACAAACAAGGAGATCGATCATACAATTTTCTACAAccc > 1:3815196/1‑70 (MQ=255)
aGTCTGATAATGCTTTTGAGATCGAAGGCTTAACAAACAAGGAGATCGATCATACAATTTTCTACAAccc > 1:3848837/1‑70 (MQ=255)
tGATAATGCTTTTGAGATCGAAGGCTTAACAAACAAGGAGATCGATCa > 1:733226/1‑48 (MQ=255)
tGATAATGCTTTTGAGATCGAAGGCTTAACAAACAAGGAGATCGATCa > 1:1567164/1‑48 (MQ=255)
tGATAATGCTTTTGAGATCGAAGGCTTAACAAACAAGGAGATCGATCa > 1:859233/1‑48 (MQ=255)
tGATAATGCTTTTGAGATCGAAGGCTTAACAAACAAGGAGATCGATCa > 1:1144402/1‑48 (MQ=255)
gATAATGCTTTTGAGATCGAAGGCTTAACAAACAAGGAgatcg < 1:2171968/43‑1 (MQ=255)
gATAATGCTTTTGAGATCGAAGGCTTAACAAACAAGGAgatcg < 1:1424697/43‑1 (MQ=255)
gATAATGCTTTTGAGATCGAAGGCTTAACAAACAAGGAgatcg < 1:3500623/43‑1 (MQ=255)
gATAATGCTTTTGAGATCGAAGGCTTAACAAACAAGGAgatcg < 1:337285/43‑1 (MQ=255)
tAATGCTTTTGAGATCGAAGGCTTAACAAACAAGGAgatcgat > 1:931437/1‑43 (MQ=255)
tAATGCTTTTGAGATCGAAGGCTTAACAAACAAGGAgatcgat > 1:1651789/1‑43 (MQ=255)
tAATGCTTTTGAGATCGAAGGCTTAACAAACAAGGAgatcgat > 1:862547/1‑43 (MQ=255)
tAATGCTTTTGAGATCGAAGGCTTAACAAACAAGGAgatcgat > 1:2949000/1‑43 (MQ=255)
tAATGCTTTTGAGATCGAAGGCTTAACAAACAAGGAgatcgat > 1:1932483/1‑43 (MQ=255)
tAATGCTTTTGAGATCGAAGGCTTAACAAACAAGGAgatcgat > 1:2790529/1‑43 (MQ=255)
tAATGCTTTTGAGATCAAAGGCTTAACAAACAAGGAgatcgat > 1:1608577/1‑43 (MQ=38)
gaTCGAAGGCTTAGCAAACAAGGAGATCGATCATGCAATTTTCTACAACCCCAACTCTGGAAGGCCAGAcc < 1:3936015/71‑1 (MQ=255)
gaTCGAAGGCTTAGCAAACAAGGAGATCGATCATGCAATTTTCTACAACCCCAACTCTGGAAGGCCAGAcc < 1:1746137/71‑1 (MQ=255)
ttAACAAACAAGGAGATCGATCATGCAATTTTCTACAACCCCAACTCTGGAAGGCCAGACCATCGTTGaa < 1:3654874/70‑1 (MQ=255)
ttAACAAACAAGGAGATCGATCATGCAATTTTCTACAACCCCAACTCTGGAAGGCCAGACCATCGTTGaa < 1:2162458/70‑1 (MQ=255)
|
TAATAATGATATCCTTTCAATAATAGCGTATCAGTCTGATAATGCTTTTGAGATCGAAGGCTTAGCAAACAAGGAGATCGATCATGCAATTTTCTACAACCCCAACTCTGGAAGGCCAGACCATCGTTGAA > W3110S.gb/904932‑905062
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
