Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F21 I0 R2	308	27.9	1975068	96.7%	1909890	67.2

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	W3110S.gb	2,916,386	G→A	100%	E892K (GAA→AAA)	barA →	hybrid sensory histidine kinase, in two‑component regulatory system with UvrY

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	2,916,386	0	G	A	77.8%	49.4 / 12.0	27	E892K (GAA→AAA)	barA	hybrid sensory histidine kinase, in two‑component regulatory system with UvrY
Reads supporting (aligned to +/- strand): ref base G (4/2); new base A (6/15); total (10/17)
Fisher's exact test for biased strand distribution p-value = 1.53e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.79e-01
Rejected as polymorphism: Polymorphic base substitution creates a homopolymer stretch.

CGTATGAAGAATCTCTGCCAACTGATCGAACAACAGCTACGTAGCGGTACTAAAGAAGAAGATTTGGAACCGGAGCTGCTGGAACTGTTGGACGAGATGGATAATGTCGCGCGCGAAGC  >  W3110S.gb/2916332‑2916450

cGTATGAAGAATCTCTGCCAACTGATCGAACAACAGCTACGTAGCGGTACTAAAGAAGAAGATTTGGAAcc                                                  >  1:1126026/1‑71 (MQ=255)

  tATGAAGAATCTCTGCCAACTGATCGAACAACAGCTACGTAGCGGTACTAAAAAAGAAGATTTGGAACCgg                                                <  1:442949/71‑1 (MQ=255)

                      tGATCGAACAACAGCTACGTAGCGGTACTAAAAAAGAAGATTTGGAACCGGAGctgctg                                        >  1:194725/1‑59 (MQ=255)

                      tGATCGAACAACAGCTACGTAGCGGTACTAAAAAAGAAGATTTGGAACCGGAGCTGCTGGAACTGTTGGAc                            >  1:111141/1‑71 (MQ=255)

                      tGATCGAACAACAGCTACGTAGCGGTACTAAAAAAGAAGATTTGGAACCGGAGCTGCTGGAACTGTTGGAc                            >  1:1674602/1‑71 (MQ=255)

                        aTCGAACAACAGCTACGTAGCGGTACTAAAAAAGAAGATTTGGAACCGGAGCTGCTGGAACTGTTGGAc                            >  1:1058682/1‑69 (MQ=255)

                        aTCGAACAACAGCTACGTAGCGGTACTAAAAAAGAAGATTTGGAACCGGAGCTGCTGGAACTGTTGGAc                            >  1:852775/1‑69 (MQ=255)

                        aTCGAACAACAGCTACGTAGCGGTACTAAAAAAGAAGATTTGGAACCGGAGCTGCTGGAACTGTTGGAc                            >  1:1296899/1‑69 (MQ=255)

                        aTCGAACAACAGCTACGTAGCGGTACTAAAAAAGAAGATTTGGAACCGGAGCTGCTGGAACTGTTGGACga                          <  1:820219/71‑1 (MQ=255)

                        aTCGAACAACAGCTACGTAGCGGTACTAAAAAAGAAGATTTGGAACCGGAGCTGCTGGAACTGTTGGACga                          <  1:1955416/71‑1 (MQ=255)

                        aTCGAACAACAGCTACGTAGCGGTACTAAAAAAGAAGATTTGGAACCGGAGCTGCAGGAACTGTTGGACga                          <  1:480987/71‑1 (MQ=255)

                         tCGAACAACAGCTACGTAGCGGTACTAAAAAAGAAGATTTGGAACCGGAGCTGCTGGAACTGTTGGACga                          <  1:133056/70‑1 (MQ=255)

                         tCGAACAACAGCTACGTAGCGGTACTAAAAAAGAAGATTTGGAACCGGAGCTGCTGGAACTGTTGGACga                          <  1:1101086/70‑1 (MQ=255)

                               aacaGCTACGTAGCGGTACTAAAGAAGAAGATTTGGAACCGGAGCTGCTGGAACTGTTGGACGAGATGGAt                   <  1:1399222/71‑1 (MQ=255)

                               aacaGCTACGTAGCGGTACTAAAAAAGAAGATTTGGAACCGGAGCTGCTGGAACTGTTGGACGAGATGGAt                   <  1:903439/71‑1 (MQ=255)

                               aacaGCTACGTAGCGGTACTAAAAAAGAAGATTTGGAACCGGAGCTGCTGGAACTGTTGGACGAGATGGAt                   <  1:595040/71‑1 (MQ=255)

                               aacaGCTACGTAGCGGTACTAAAAAAGAAGATTTGGAACCGGAGCTGCTGGAACTGTTGGACGAGATGGAt                   <  1:1849722/71‑1 (MQ=255)

                               aacaGCTACGTAGCGGTACTAAAAAAGAAGATTTGGAACCGGAGCTGCTGGAACTGTTGGACGAGATGGAt                   <  1:396484/71‑1 (MQ=255)

                               aacaGCTACGTAGCGGTACTAAAAAAGAAGATTTGGAACCGGAGCTGCTGGAACTGTTGGACGAGATGGAt                   <  1:230063/71‑1 (MQ=255)

                               aacaGCTACGTAGCGGTACTAAAAAAGAAGATTTGGAACCGGAGCTGCTGGAACTGTTGGACGAGATGGAt                   <  1:1836496/71‑1 (MQ=255)

                               aacaGCTACGTAGCGGTACTAAAAAAGAAGATTTGGAACCGGAGCTGCTGGAACTGTTGGACGAGATGGAt                   <  1:157657/71‑1 (MQ=255)

                               aacaGCTACGTAGCGGTACTAAAAAAGAAGATTTGGAACCGGAGCTGCTGGAACTGTTGGACGAGATGGAt                   <  1:1544058/71‑1 (MQ=255)

                                   gCTACGTAGCGGTACTAAAGAAGAAGATTTGGAACCGGAGCTGCTGGAACTGTTGGACGAGATGGAt                   <  1:1514287/67‑1 (MQ=255)

                                               tACTAAAAAAGAAGATTTGGAACCGGAGCTGCTGGAACTGTTGGACga                          <  1:895244/48‑1 (MQ=255)

                                                 cTAAAGAAGAAGATTTGGAACCGGAGCTGCTGGAACTGTTGGACGAGATGGATAATGTCGCGCGCGAAGc  >  1:1053581/1‑70 (MQ=255)

                                                 cTAAAGAAGAAGATTTGGAACCGGAGCTGCTGGAACTGTTGGACGAGATGGATAATGTCGCGCGCGAAGc  >  1:67827/1‑70 (MQ=255)

                                                 cTAAAGAAGAAGATTTGGAACCGGAGCTGCTGGAACTGTTGGACGAGATGGATAATGTCGCGCGCGAAGc  >  1:932247/1‑70 (MQ=255)

CGTATGAAGAATCTCTGCCAACTGATCGAACAACAGCTACGTAGCGGTACTAAAGAAGAAGATTTGGAACCGGAGCTGCTGGAACTGTTGGACGAGATGGATAATGTCGCGCGCGAAGC  >  W3110S.gb/2916332‑2916450

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

N/A