Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F28 I3 R2
|
122 |
64.1 |
3058059 |
97.6% |
2984665 |
63.3 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
minE |
2,029,965 |
A→G |
F294L (TTC→CTC) |
parE ← |
DNA topoisomerase IV, subunit B |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 2,029,965 | 0 | A | G | 100.0%
| 240.2
/ NA
| 67 | F294L (TTC→CTC) | parE | DNA topoisomerase IV, subunit B |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (43/24); total (43/24) |
GCAGCGATCCCAGATATCTTCCGCCGACAGCTTTACACCGCGCGGCAGAATATTGCGGTATTCACAGAACTCACGCATCGCGTCCAACAGGCCCTGACGCAGACCATTAACATGGGTACCGCCCTGCATCGTTGG > minE/2029897‑2030031
|
gcAGCGATCCCAGATATCTTCCGCCGACAGCTTTACACCGCGCGGCAGAATATTGCGGTATTCACAGAGCt > 1:1257717/1‑71 (MQ=255)
cAGCGATCCCAGATATCTTCCGCCGACAGCTTTACACCGCGCGGCAGAATATTGCGGTATTCACAGAGCt < 1:2798330/70‑1 (MQ=255)
tCCCAGATATCTTCCGCCGACAGCTTTACACCGCGCGGCAGAATATTGCGGTATTCACAGAGCTCACGCAt < 1:715355/71‑1 (MQ=255)
tCCCAGATATCTTCCGCCGACAGCTTTACACCGCGCGGCAGAATATTGCGGTATTCACAGAGCTCACGCAt > 1:2985730/1‑71 (MQ=255)
tCCCAGATATCTTCCGCCGACAGCTTTACACCGCGCGGCAGAATATTGCGGTATTCACAGAGCTCACGCAt > 1:774577/1‑71 (MQ=255)
cccAGATATCTTCCGCCGACAGCTTTACACCGCGCGGCAGAATATTGCGGTATTCACAGAGCTCACGCATc > 1:1411372/1‑71 (MQ=255)
ccATATATCTTCCGCCGACAGCTTTACACCGCGCGGCAGAATATTGCGGTATTCACAGAGCt < 1:245141/62‑1 (MQ=255)
ccAGATATCTTCCGCCGACAGCTTTACACCGCGCGGCAGAATATTGCGGTATTCACAGAGCTCACGCATc < 1:456/70‑1 (MQ=255)
aGATATCTTCCGCCGACAGCTTTACACCGCGCGGCAGAATATTGCGGTATTCACAGAGCTCACGCATcgcg > 1:1831079/1‑71 (MQ=255)
aGATATCTTCCGCCGACAGCTTTACACCGCGCGGCAGAATATTGCGGTATTCACAGAGCTCACGCATcgcg > 1:6352/1‑71 (MQ=255)
atatCTTCCGCCGACAGCTTTACACCGCGCGGCAGAATATTGCGGTATTCACAGAGCTCACGCATCGCGTc > 1:1326186/1‑71 (MQ=255)
atatCTTCCGCCGACAGCTTTACACCGCGCGGCAGAATATTGCGGTATTCACAGAGCTCACGCATCGCGTc > 1:3050064/1‑71 (MQ=255)
tCTTCCGCCGACAGCTTTACACCGCGCGGCAGAATATTGCGGTATTCACAGAGCTCACGCATCGCGTCCaa > 1:2453223/1‑71 (MQ=255)
ccgccgACAGCTTTACACCGCGCGGCAGAATATTGCGGTATTCACAGAGCTCACGCATCGCGTCCAAc < 1:1674876/68‑1 (MQ=255)
ccgccgACAGCTTTACACCGCGCGGCAGAATATTGCGGTATTCACAGAGCTCACGCATCGCGTCCAACa < 1:2954413/69‑1 (MQ=255)
ccgccgACAGCTTTACACCGCGCGGCAGAATATTGCGGTATTCACAGAGCTCACGCATCGCGTCCAACAgg > 1:2593041/1‑71 (MQ=255)
gccgACAGCTTTACACCGCGCGGCAGAATATTGCGGTATTCACAGAGCTCACGCATCGCGTCCAACAGGcc > 1:2154948/1‑71 (MQ=255)
gACAGATTTACACCGCGCGGCAGAGTATTGCGGTATTCACAGAGCTCACGCATCGCGTCCAACAGGCCCTg < 1:1643594/71‑1 (MQ=255)
aCAGCTTTACACCGCGCGGCAGAATATTGCGGTATTCACAGAGCTCa > 1:2585920/1‑47 (MQ=255)
aCAGCTTTACACCGCGCGGCAGAATATTGCGGTATTCACAGAGCTCa > 1:758462/1‑47 (MQ=255)
aCAGCTTTACACCGCGCGGCAGAATATTGCGGTATTCACAGAGCTCa > 1:640443/1‑47 (MQ=255)
cAGCTTTACACCGCGCGGCAGAATATTGCGGTATTCACAGAGCTCACGCATCGCGTCCAACAGGCCCTGa < 1:2553557/70‑1 (MQ=255)
cAGCTTTACACAGCGCGGCAGAATATTGCGGTATTCACAGAGCTCACGCATCGCGTCCAACAGGCCCTGAc > 1:2386236/1‑71 (MQ=255)
aGCTTTACACCGCGCGGCAGAATATTGCGGTATTCACAGAGCTCACGCATCGCGTCCAACAGGCCCTGACg > 1:442011/1‑71 (MQ=255)
cTTTACACCGCGCGGCAGAATATTGCGGTATTCACAGAGCTCACGCATCGCGTCCAACAGGCCCTGACg > 1:2809091/1‑69 (MQ=255)
cTTTACACCGCGCGGCAGAATATTGCGGTATTCACAGAGCTCACGCATCGCGTCCAACAGGCCCTGACg > 1:1130606/1‑69 (MQ=255)
cTTTACACCGCGCGGCAGAATATTGCGGTATTCACAGAGCTCACGCATCGCGTCCAACAGGCCCTGACGCa < 1:1860354/71‑1 (MQ=255)
ttACACCGCGCGGCAGAATATTGCGGTATTCACAGAGCTCACGCCTCGCGTCCAAc > 1:2388037/1‑56 (MQ=255)
tACACCGCGCGGCAGAATATTGCGGTATTCACAGAGCTCACGc > 1:1359874/1‑43 (MQ=255)
tACACCGCGCGGCAGAATATTGCGGTATTCACAGAGCTCACGCATCGCGTCCAACAGGCCCTTACGCAg < 1:392296/69‑1 (MQ=255)
acacCGCGCGGCAGAATATTGCGGTATTCACAGAGCTCACGCATCGCGTCCAACAGGc > 1:2263403/1‑58 (MQ=255)
acCGCGCGGCAGAATATTGCGGTATTCACAGAGCTCACGCATCGCGTCCAACAGGCCCTGACGCAGACCAt < 1:643579/71‑1 (MQ=255)
ccGCGCGGCAGAATATTGCGGTATTCACAGAGCTCACGCATCGCGTCCAACAGGCCCTGACGCAGACCAt > 1:280407/1‑70 (MQ=255)
cgcgcgGCAGAATATTGCGGTATTCACAGAGCTCACGCATCGCGTCCAACAGGCCCTGACGCAGACCAtt < 1:488804/70‑1 (MQ=255)
gcgcgGCAGAATATTGCGGTATTCACAGAGCTCACGCATCGCGTCCAACAGGCCCTGACGCAGACCATTaa > 1:2339294/1‑71 (MQ=255)
gcgcgGCAGAATATTGCGGTATTCACAGAGCTCACGCATCGCGTCCAACAGGCCCTGACGCAGACCATTa > 1:1371982/1‑70 (MQ=255)
cgcgGCAGAATATTGCGGTATTCACAGAGCTCACGCATTGCGTCCAACAGGCCCTGACGCAGACCa > 1:2167151/1‑66 (MQ=255)
cgcgGCAGAATATTGCGGTATTCACAGAGCTCACGCATCGCGTCCAACAGGCCCTGACGCAGACCATTaa > 1:2740013/1‑70 (MQ=255)
cgcgGCAGAATATTGCGGTATTCACAGAGCTCACGCATCGCGTCCAACAGGCCCTGACGCAGACCATTa > 1:2909853/1‑69 (MQ=255)
gcgGCAGAATATTGCGGTATTCACAGAGCTCACGCATCGCGTcc < 1:130774/44‑1 (MQ=255)
gCAGAATATTGCGGTATTCACAGAGCTCACGCATCGCGTCCAACAGGCCCTGACGCAGACCATTAACATgg < 1:2425017/71‑1 (MQ=255)
cAGAATATTGCGGTATTCACAGAGCTCACGCATCGCGTCCAACAGGCCCTGACGCAGACCATTAACATggg < 1:144741/71‑1 (MQ=255)
cAGAATATTGCGGTATTCACAGAGCTCACGCATCGCGTCCAACAGGCCCTGACGCAGACCATTAACATggg > 1:2475971/1‑71 (MQ=255)
cAGAATATTGCGGTATTCACAGAGCTCACGCATCGCGTCCAACAGGCCCTGACGCAGACCATTAACATggg > 1:838781/1‑71 (MQ=255)
cAGAATATTGCGGTATTCACAGAGCTCACGCATCGCGTCCAACAGGCCCTGACGCAGACCATTAACATggg > 1:1490108/1‑71 (MQ=255)
aaTATTGCGGTATTCACAGAGCTCACGCATCGCGTCCAACAGGCCCTGACGCAGACCATTAACATGGGTAc > 1:1190868/1‑71 (MQ=255)
tatTGCGGTATTCACAGAGCTCACGCATCGCGTCCa > 1:1755699/1‑36 (MQ=255)
tatTGCGGTATTCACAGAGCTCACGCATCGCGTCCa > 1:636375/1‑36 (MQ=255)
ttGCGGTATTCACAGAGCTCACGCATCGCGTCCAACAGGCCCTGACGCAGACCATTAACa > 1:1549778/1‑60 (MQ=255)
ttGCGGTATTCACAGAGCTCACGCATCGCGTCCAACAGGCCCTGACGCAGACCATTAACa > 1:1965014/1‑60 (MQ=255)
ttGCGGTATTCACAGAGCTCACGCATCGCGTCCAACAGGCCCTGACGCAGACCATTAACATGGGTACCGcc < 1:889815/71‑1 (MQ=255)
tGCGGTATTCACAGAGCTCACGCATCGCGTCCAACAGGCCCTGACGCAGACCAtt < 1:2894690/55‑1 (MQ=255)
tGCGGTATTCACAGAGCTCACGCATCGCGTCCAACAGGCCCTGACGCAGACCATTAACATGGGTACCGcc > 1:604073/1‑70 (MQ=255)
tGCGGTATTCACAGAGCTCACGCATCGCGTCCAACAGGCCCTGACGCAGACCATTAACATGGGTACCGcc > 1:226500/1‑70 (MQ=255)
gCGGTATTCACAGAGCTCACGCATCGCGTCCAACAGGCCCTGa > 1:1114297/1‑43 (MQ=255)
ggTATTCACAGAGCTCACGCATCGCGTCCAACAGGCCCTGACGCAGACCATTAACATGGGTACCGcc > 1:404108/1‑67 (MQ=255)
tATTCACAGAGCTCACGCATCGCGTCCAACAGGCCCTGACGCa < 1:890599/43‑1 (MQ=255)
ttCACAGAGCTCACGCATCGCGTCCAACAGGCCCTGACGCAGACCATTAACATGGGTACCGCCCTGCATCg > 1:2343320/1‑71 (MQ=255)
ttCACAGAGCTCACGCATCGCGTCCAACAGGCCCTGACGCAGACCATTAACATGGGTACCGCCCTGCATCg > 1:2278454/1‑71 (MQ=255)
ttCACAGAGCTCACGCATCGCGTCCAACAGGCCCTGACGCAGACCATTAACATGGGTACCGCCCTGCATCg < 1:1893464/71‑1 (MQ=255)
ttCACAGAGCTCACGCATCGCGTCCAACAGGCCCTGACGCAGACCATTAACATGGGTACCGCCCTGCATCg > 1:1493297/1‑71 (MQ=255)
cacaGAGCTCACGCATCGCGTCCAACAGGCCCTGa > 1:787580/1‑35 (MQ=255)
acaGAGCTCACGCATCGCGTCCAACAGGCCCTGACGCAGACCATTAACATGGGTACCGCCCTGCATCGtt < 1:2961119/70‑1 (MQ=255)
acaGAGCTCACGCATCGCGTCCAACAGGCCCTGACGCAGACCATTAACATGGGTACCGCCCTGCATCGtt < 1:1642032/70‑1 (MQ=255)
caGAGCTCACGCATCGCGTCCAACAGGCCCTGACGCAGACCAtt < 1:2956428/44‑1 (MQ=255)
caGAGCTCACGCATCGCGTCCAACAGGCCCTGACGCAGACCATTAACATGGGTACCGCCCTGCATCGTTgg < 1:2498767/71‑1 (MQ=255)
caGAGCTCACGCATCGCGTCCAACAGGCCCTGACGCAGACCATTAACATGGGTACCGCCCTGCATCGTTgg < 1:108301/71‑1 (MQ=255)
agagCTCACGCATCGCGTCCAACAGGCCCTGACGCAGACCATTAACATGGGTACCGCCCTGCATCGTTgg > 1:64594/1‑70 (MQ=255)
|
GCAGCGATCCCAGATATCTTCCGCCGACAGCTTTACACCGCGCGGCAGAATATTGCGGTATTCACAGAACTCACGCATCGCGTCCAACAGGCCCTGACGCAGACCATTAACATGGGTACCGCCCTGCATCGTTGG > minE/2029897‑2030031
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
