Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F28 I1 R2
|
328 |
63.0 |
4687567 |
98.0% |
4593815 |
65.2 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
505,179 |
C→T |
L348L (CTG→TTG) |
ushA → |
bifunctional UDP‑sugar hydrolase and 5'‑nucleotidase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 505,179 | 0 | C | T | 100.0%
| 212.0
/ NA
| 70 | L348L (CTG→TTG) | ushA | bifunctional UDP‑sugar hydrolase and 5'‑nucleotidase |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (38/32); total (38/32) |
GAAGACGGGAAAAGCGAGCGCGTGCTTTACACTCCTGAAATCGCTGAAAACCAGCAAATGATCTCGCTGTTATCACCGTTCCAGAACAAAGGCAAAGCGCAGCTGGAAGTGAAAATAGGCGAAACCAATGGTC > W3110S.gb/505113‑505245
|
gAAGACGGGAAAAGCGAGCGCGTGCTTTACACTCCTGAAATCGCTGAAAACCAGCAAATGATCTCgttgtt < 1:2573379/71‑1 (MQ=255)
gAAGACGGGAAAAGCGAGCGCGTGCTTTACACTCCTGAAATCGCTGAAAACCAGCAAATGATCTCgttgtt < 1:187379/71‑1 (MQ=255)
aCGGGAAAAGCGAGCGCGTGCTTTACACTCCTGAAATCGCTGAAAACCAGCAAATGATCTCGTTGTTATCa > 1:2488252/1‑71 (MQ=255)
aCGGGAAAAGCGAGAGCGTGCTTTACACTCCTGAAATCGCTGAAAACCAGCAAATGATCTCGTTGTTAt > 1:3282480/1‑69 (MQ=255)
cGGGAAAAGCGAGCGCGTGCTTTACACTCCTGAAATCGCTGAAAACCAGCAAATGATCTCGTTGTTATCAc < 1:86158/71‑1 (MQ=255)
aaaGCGAGCGCGTGCTTTACACTCCTGAAATCGCTGAAAACCAGCAAATGATCTCGTTGTTATCACCGTTc > 1:1947783/1‑71 (MQ=255)
gagcgCGTGCTTTACACTCCTGAAATCGCTGAAAACCAGCAAATGATCTCGTTGTTATCACCGTTCCAGaa < 1:1658637/71‑1 (MQ=255)
gcgcGTGCTTTACACTCCTGAAATCGCTGAAAACCAGCAAATGATCTCGTTGTTATCACCGTTCCAGAACa < 1:612266/71‑1 (MQ=255)
gcgTGCTTTACACTCCTGAAATCGCTGAAAACCAGCAAATGATCTCGTTGTTATCACCGt < 1:371229/60‑1 (MQ=255)
cTTTACACTCCTGAAATCGCTGAAAACCAGCAAATGATCTCGTTGTTATCACCGTTCCAGAACAAAGGCa > 1:1423851/1‑70 (MQ=255)
cTTTACACTCCTGAAATCGCTGAAAACCAGCAAATGATCTCGTTGTTATCACCGTTCCAGAACAAAGGCa > 1:3924463/1‑70 (MQ=255)
cTTTACACTCCTGAAATCGCTGAAAACCAGCAAATGATCTCGTTGTTATCACCGTTCCAGAACAAAGGCa > 1:4234196/1‑70 (MQ=255)
cTTTACACTCCTGAAATCGCTGAAAACCAGCAAATGATCTCGTTGTTATCACCGTTCCAGAACAAAGGCa > 1:447706/1‑70 (MQ=255)
cTTTACACTCCTGAAATCGCTGAAAACCAGCAAATGATCTCGTTGTTATCACCGTTCCAGAACAAAGGCa > 1:4285346/1‑70 (MQ=255)
tttACACTCCTGAAATCGCTGAAAACCAGCAAATGATCTCGTTGTTATCACCGTTCCAGAACAAAGGCa < 1:442992/69‑1 (MQ=255)
ttACACTCCTGAAATCGCTGAAAACCAGCAAATGATCTCGTTGTTATCACCGTTCCAGAACAAAGGCAAAg > 1:1301789/1‑71 (MQ=255)
tACACTCCTGAAATCGCTGAAAACCAGCAAATGATCTCGTTGTTa > 1:3888145/1‑45 (MQ=255)
tACACTCCTGAAATCGCTGAAAACCAGCAAATGATCTCGTTGTTa > 1:2290884/1‑45 (MQ=255)
tACACTCCTGAAATCGCTGAAAACCAGCAAATGATCTCGTTGTTa > 1:1120096/1‑45 (MQ=255)
tACACTCCTGAAATCGCTGAAAACCAGCAAATGATCTCGTTGTTATCACCGTTCCAGAACAAAGGCAAAgc > 1:966463/1‑71 (MQ=255)
cTCCTGAAATCGCTGAAAACCAGCAAATGATCTCGTTGTTATCAc < 1:2155771/45‑1 (MQ=255)
cTCCTGAAATCGCTGAAAACCAGCAAATGATCTCGTTGTTATCAc < 1:3738066/45‑1 (MQ=255)
cTCCTGAAATCGCTGAAAACCAGCAAATGATCTCGTTGTTATCACCGTTCCAGAACAAAGGCAAAGCGCAg > 1:879178/1‑71 (MQ=255)
ccTGAAATCGCTGAAAACCAGCAAATGATCTCGTTGTTAt > 1:1632378/1‑40 (MQ=255)
ccTGAAATCGCTGAAAACCAGCAAATGATCTCGTTGTTAt > 1:4150682/1‑40 (MQ=255)
ccTGAAATCGCTGAAAACCAGCAAATGATCTCGTTGTTATCACCGTTCCAGAACAAAGGCaa > 1:4431806/1‑62 (MQ=255)
ccTGAAATCGCTGAAAACCAGCAAATGATCTCGTTGTTATCACCGTTCCAGAACAAAGGCaa > 1:4656383/1‑62 (MQ=255)
ccTGAAATCGCTGAAAACCAGCAAATGATCTCGTTGTTATCACCGTTCCAGAACAAAGGCAAAg > 1:1646738/1‑64 (MQ=255)
ccTGAAATCGCTGAAAACCAGCAAATGATCTCGTTGTTATCACCGTTCCAGAACAAAGGCAAAGCGCAGCt > 1:1126427/1‑71 (MQ=255)
ccTGAAATCGCTGAAAACCAGCAAATGATCTCGTTGTTATCACCGTTCCAGAACAAAGGCAAAGCGCAGCt > 1:753443/1‑71 (MQ=255)
ccTGAAATCGCTGAAAACCAGCAAATGATCTCGTTGTTATCACCGTTCCAGAACAAAGGCAAAGCGCAGCt > 1:3300584/1‑71 (MQ=255)
cTGAAATCGCTGAAAACCAGCAAATGATCTCgttgtt < 1:4155832/37‑1 (MQ=255)
cTGAAATCGCTGAAAACCAGCAAATGATCTCGTTGTTATCACCGTTCCAGAACAAAGGCAAAGCGCAGCTg > 1:3176167/1‑71 (MQ=255)
cTGAAATCGCTGAAAACCAGCAAATGATCTCGTTGTTATCACCGTTCCAGAACAAAGGCAAAGCGCAGCTg > 1:4162792/1‑71 (MQ=255)
gAAATCGCTGAAAACCAGCAAATGATCTCGTTGTTAt > 1:3224492/1‑37 (MQ=255)
gAAATCGCTGAAAACCAGCAAATGATCTCGTTGTTAt > 1:2410471/1‑37 (MQ=255)
gAAATCGCTGAAAACCAGCAAATGATCTCGTTGTTAt > 1:3356312/1‑37 (MQ=255)
aaaTCGCTGAAAACCAGCAAATGATCTCGTTGTTATCACCGTTCCAGAACAAAGGCAAAGCGCAGCTgg < 1:3026874/69‑1 (MQ=255)
aaaTCGCTGAAAACCAGCAAATGATCTCGTTGTTATCACCGTTCCAGAACAAAGGCAAAGCGCAGCTgg < 1:3212680/69‑1 (MQ=255)
aaTCGCTGAAAACCAGCAAATGATCTCGTTGTTATCACCGt < 1:1523859/41‑1 (MQ=255)
aTCGCTGAAAACCAGCAAATGATCTCGTTGTTATCACCGTTCCAGAACAAAGGCAAAGCGCAGCTGGAAGt > 1:1753219/1‑71 (MQ=255)
tCGCTGAAAACCAGCAAATGATCTCGTTGTTATCACCGTTCCAGAACAAAGGCAAAGCGCAGCTGGAAGt > 1:2196764/1‑70 (MQ=255)
tCGCTGAAAACCAGCAAATGATCTCGTTGTTATCACCGTTCCAGAACAAAGGCAAAGCGCAGCTGGAAGt > 1:4362094/1‑70 (MQ=255)
cGCTGAAAACCAGCAAATGATCTCGTTGTTATCACCGTTCCAGAACAAAGGCAAAGCGCAGCTGGAAGTg > 1:147045/1‑70 (MQ=255)
cGCTGAAAACCAGCAAATGATCTCGTTGTTATCACCGTTCCAGAACAAAGGCAAAGCGCAGCTGGAAGTg > 1:3800750/1‑70 (MQ=255)
cGCTGAAAACCAGCAAATGATCTCGTTGTTATCACCGTTCCAGAACAAAGGCAAAGCGCAGCTGGAAGTg > 1:2373091/1‑70 (MQ=255)
gCTGAAAACCAGCAAATGATCTCGTTGTTATCACCGTTCCAGAACAAAGGCAAAGCTCAGCTGGAAGTGaa < 1:3394397/71‑1 (MQ=255)
gCTGAAAACCAGCAAATGATCTCGTTGTTATCACCGTTCCAGAACAAAGGCAAAGCGCAGCTGGAAGTGaa < 1:425684/71‑1 (MQ=255)
gCTGAAAACCAGCAAATGATCTCGTTGTTATCACCGTTCCAGAACAAAGGCAAAGCGCAGCTGGAAGTGaa < 1:1868419/71‑1 (MQ=255)
gCTGAAAACCAGCAAATGATCTCGTTGTTATCACCGTTCCAGAACAAAGGCAAAGCGCAGCTGGAAGTGaa < 1:537071/71‑1 (MQ=255)
gCTGAAAACCAGCAAATGATCTCGTTGTTATCACCGTTCCAGAACAAAGGCAAAGCGCAGCTGGAAGTGaa < 1:2102062/71‑1 (MQ=255)
gCTGAAAACCAGCAAATGATCTCGTTGTTATCACCGTTCCAGAACAAAGGCAAAGCGCAGCTGGAAGTGaa < 1:2222414/71‑1 (MQ=255)
gCTGAAAACCAGCAAATGATCTCGTTGTTATCACCGTTCCAGAACAAAGGCAAAGCGCAGCTGGAAGTGaa < 1:3630540/71‑1 (MQ=255)
gCTGAAAACCAGCAAATGATCTCGTTGTTATCACCGTTCCAGAACAAAGGCAAAGCGCAGCTGGAAGTGaa < 1:320887/71‑1 (MQ=255)
aaaaCCAGCAAATGATCTCGTTGTTATCACCGTTCCAGAACAAAGGCAAAGCGCAGCTGGAAGTGaaaa < 1:829536/69‑1 (MQ=255)
aaaaCCAGCAAATGATCTCGTTGTTATCACCGTTCCAGAACAAAGGCAAAGCGCAGCTGGAAGTGaaaa < 1:4437473/69‑1 (MQ=255)
aaaaCCAGCAAATGATCTCGTTGTTATCACCGTTCCAGAACAAAGGCAAAGCGCAGCTGGAAGTGaaaa < 1:4553659/69‑1 (MQ=255)
aaaCCAGCAAATGATCTCGTTGTTATCACCGTTCCAGAACAAAGGCAAAGCGCAGCTGGAAGTGAAAATAg < 1:3820088/71‑1 (MQ=255)
ccAGCAAATGATCTCGTTGTTATCACCGTTCCAGAACAAAGGCAAAGCGCAGCTGGAAGTGAAAATAGGc > 1:4044649/1‑70 (MQ=255)
gCAAATGATCTCGTTGTTATCACCGTTCCAGAACAAAGGCAAAGCGCAGCTGGAAGTGaa < 1:2347711/60‑1 (MQ=255)
aaaTGATCTCGTTGTTATCACCGTTCCAGAACAAAGGCAAAGCGCAGCTGGAAGTGAAAATAGGCGAAAcc < 1:1649933/71‑1 (MQ=255)
aaaTGATCTCGTTGTTATCACCGTTCCAGAACAAAGGCAAAGCGCAGCTGGAAGTGAAAATAGGCGAAAcc < 1:3124614/71‑1 (MQ=255)
aaTGATCTCGTTGTTATCACCGTTCCAGAACAAAGGCAAAGCGCAGCTgg < 1:1309237/50‑1 (MQ=255)
aaTGATCTCGTTGTTATCACCGTTCCAGAACAAAGGCAAAGCGCAGCTgg < 1:1785470/50‑1 (MQ=255)
aaTGATCTCGTTGTTATCACCGTTCCAGAACAAAGGCAAAGCGCAGCTgg < 1:1780153/50‑1 (MQ=255)
aaTGATCTCGTTGTTATCACCGTTCCAGAACAAAGGCAAAGCGCAGCTGGAAGTGaa < 1:2048480/57‑1 (MQ=255)
aTGATCTCGTTGTTATCACCGTTCCAGAACAAAGGCAAAGCGCAGCTGGAAGTGAAAATAGGCGAAAcc < 1:2615868/69‑1 (MQ=255)
aTCTCGTTGTTATCACCTTTCCAGAACAAAGGCAAAGCGCAg > 1:2997328/1‑42 (MQ=38)
aTCTCGTTGTTATCACCGTTCCAGAACAAAGGCAAAGCGCAGCTGGAAGTGaa < 1:1180729/53‑1 (MQ=255)
aTCTCGTTGTTATCACCGTTCCAGAACAAAGGCAAAGCGCAGCTGGAAGTGaa < 1:2419454/53‑1 (MQ=255)
aTCTCGTTGTTATCACCGTTCCAGAACAAAGGCAAAGCGCAGCTGGAAGTAAAAATAGGCGAAACCAATgg > 1:3298260/1‑71 (MQ=255)
aTCTCGTTGTTATCACCGTTCCAGAACAAAGGCAAAGCGCAGATGGAAGTGAAAATAGGCGAAACCAATgg > 1:2672497/1‑71 (MQ=255)
ctcGTTGTTATCACCGTTCCAGAACAAAGGCAAAGCGCAGCTGGAAGTGAAAATAGGCGAAACCAATGgtc > 1:1275701/1‑71 (MQ=255)
|
GAAGACGGGAAAAGCGAGCGCGTGCTTTACACTCCTGAAATCGCTGAAAACCAGCAAATGATCTCGCTGTTATCACCGTTCCAGAACAAAGGCAAAGCGCAGCTGGAAGTGAAAATAGGCGAAACCAATGGTC > W3110S.gb/505113‑505245
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
