Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F28 I1 R2
|
328 |
63.0 |
4687567 |
98.0% |
4593815 |
65.2 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
1,303,631 |
C→T |
Q246Y (CAG→TAT) |
oppA → |
oligopeptide transporter subunit |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 1,303,631 | 0 | C | T | 100.0%
| 200.6
/ NA
| 68 | Q246Y (CAG→TAT) | oppA | oligopeptide transporter subunit |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (22/46); total (22/46) |
TAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGT > W3110S.gb/1303566‑1303687
|
tAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTa < 1:579039/71‑1 (MQ=255)
tAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTa < 1:1714405/71‑1 (MQ=255)
aacgaacgAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAAc > 1:2949300/1‑71 (MQ=255)
aacgaacgAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAAc > 1:3313664/1‑71 (MQ=255)
aacgaacgAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAAc > 1:4220221/1‑71 (MQ=255)
acgaacgaATCGTTCTTGAACGCAGGCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAAcc < 1:96869/71‑1 (MQ=255)
acgaacgaATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAAcc < 1:1816859/71‑1 (MQ=255)
gaacgaaTCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCTAAAACCGTTATTAACTATGTAACCTa < 1:218479/71‑1 (MQ=255)
gaacgaaTCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTa < 1:4339830/71‑1 (MQ=255)
gaacgaaTCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTa < 1:313087/71‑1 (MQ=255)
gaacgaaTCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTa < 1:4548665/71‑1 (MQ=255)
gaacgaaTCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTa > 1:3942965/1‑71 (MQ=255)
gaacgaaTCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTa < 1:4594595/71‑1 (MQ=255)
gaacgaaTCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTa < 1:3140804/71‑1 (MQ=255)
gaacgaaTCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTa < 1:89776/71‑1 (MQ=255)
gaacgaaTCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTa < 1:2758332/71‑1 (MQ=255)
gaacgaaTCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTa < 1:2454370/71‑1 (MQ=255)
gaacgaaTCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTa < 1:2362684/71‑1 (MQ=255)
gaacgaaTCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAAATATGTAACCTa < 1:2938012/71‑1 (MQ=255)
ctaaTCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTAt < 1:2783373/67‑1 (MQ=255)
aaTCGTTC‑TCAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGcc < 1:3259904/71‑1 (MQ=255)
aTCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGcc < 1:3507448/71‑1 (MQ=255)
aTCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGcc < 1:4521602/71‑1 (MQ=255)
aTCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGcc < 1:3939997/71‑1 (MQ=255)
aTCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGcc < 1:2353667/71‑1 (MQ=255)
aTCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGcc < 1:293011/71‑1 (MQ=255)
tCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCt < 1:2654723/71‑1 (MQ=255)
tCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCt < 1:1899424/71‑1 (MQ=255)
tCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCt < 1:4211105/71‑1 (MQ=255)
tCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCt < 1:2205424/71‑1 (MQ=255)
gTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTAtt > 1:2605016/1‑64 (MQ=255)
gTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTAt > 1:4361204/1‑71 (MQ=255)
gTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATATAACCTAtt > 1:758159/1‑64 (MQ=255)
cTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGc > 1:1775636/1‑71 (MQ=255)
gAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGc < 1:1311591/68‑1 (MQ=255)
gAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGc < 1:4061581/68‑1 (MQ=255)
gAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCt < 1:330502/69‑1 (MQ=255)
cGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGa < 1:3915287/71‑1 (MQ=255)
gCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGt > 1:3528490/1‑70 (MQ=255)
ccGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTAc > 1:2357513/1‑71 (MQ=255)
ccGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTAc > 1:4151010/1‑71 (MQ=255)
ccGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTAc > 1:2339589/1‑71 (MQ=255)
cGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTAcc < 1:2141967/71‑1 (MQ=255)
cGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTAcc < 1:4627353/71‑1 (MQ=255)
cGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTAcc < 1:1082670/71‑1 (MQ=255)
cGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTAcc < 1:3754187/71‑1 (MQ=255)
cGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTAcc < 1:3718599/71‑1 (MQ=255)
cGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTAcc < 1:2399733/71‑1 (MQ=255)
cGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTAcc < 1:3140555/71‑1 (MQ=255)
cGACCTACTGGAACAACGCGAAAACCGTTACTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTAcc < 1:1482630/71‑1 (MQ=255)
aCCTACTTGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGa < 1:3498553/71‑1 (MQ=255)
cTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGt > 1:2507970/1‑69 (MQ=255)
cTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTc > 1:2809164/1‑70 (MQ=255)
cTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTc > 1:1478980/1‑70 (MQ=255)
cTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTc > 1:300105/1‑70 (MQ=255)
cTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTc > 1:2678875/1‑70 (MQ=255)
cTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTc > 1:2529195/1‑70 (MQ=255)
cTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTc > 1:3814326/1‑70 (MQ=255)
cTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCa < 1:763548/71‑1 (MQ=255)
ggAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTTCCTATTGCTTCTGAAGTTACCGATGTCAAc > 1:1046094/1‑71 (MQ=255)
acaacGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGa > 1:863010/1‑61 (MQ=255)
acaacGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGa > 1:259265/1‑61 (MQ=255)
cgAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGt < 1:2723949/71‑1 (MQ=255)
cgAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGt < 1:3607408/71‑1 (MQ=255)
aaaCCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAgt < 1:2486592/71‑1 (MQ=255)
aaCCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAgt < 1:3154808/70‑1 (MQ=255)
ccGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCg < 1:1727236/48‑1 (MQ=39)
ccGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCg < 1:92911/48‑1 (MQ=39)
|
TAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGT > W3110S.gb/1303566‑1303687
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
