Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F28 I1 R2
|
328 |
63.0 |
4687567 |
98.0% |
4593815 |
65.2 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
1,558,725 |
G→A |
Q129Q (CAG→CAA) |
osmC → |
osmotically inducible, stress‑inducible membrane protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 1,558,725 | 0 | G | A | 100.0%
| 157.6
/ NA
| 53 | Q129Q (CAG→CAA) | osmC | osmotically inducible, stress‑inducible membrane protein |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (28/25); total (28/25) |
CCGGGTATTGATGCCTCTACCTTTGACGGCATAATCCAGAAAGCAAAAGCAGGATGCCCGGTCTCTCAGGTACTGAAAGCGGAAATTACGCTGGATTACCAGTTGAAATCGTAAAGCATTGCCGGATGACGCGT > W3110S.gb/1558657‑1558790
|
ccGGGTATTGATGCCTCTACCTTTGACGGCATAATCCAGAAAGCAAAAGCAGGATGCCCGGTCTCTCAAGt < 1:751232/71‑1 (MQ=255)
tATTGATGCCTCTACCTTTGACGGCATAATCCAGAAAGGAAAAGCAGGATGCCCGGTCTCTCAAGTACTg > 1:1006134/1‑70 (MQ=255)
gATGCCTCTACCTTTGACGGCATAATCCAGAAAGCAAAAGCAGGATGCCCGGTCTCTCAAGTACTGAAAGc > 1:3227187/1‑71 (MQ=255)
gATGCCTCTACCTTTGACGGCATAATCCAGAAAGCAAAAGCAGGATGCCCGGTCTCTCAAGTACTGAAAGc > 1:2443679/1‑71 (MQ=255)
gATGCCTCTACCTTTGACGGCATAATCCAGAAAGCAAAAGCAGGATGCCCGGTCTCTCAAGTACTGAAAGc > 1:2041848/1‑71 (MQ=255)
ccTCTACCTTTGACGGCATAATCCAGAAAGCAAAAGCAGGATGCCCGGTCTCTCAAGTACTGAAAGCGGa < 1:3062058/70‑1 (MQ=255)
ccTCTACCTTTGACGGCATAATCCAGAAAGCAAAAGCAGGATGCCCGGTCTCTCAAGTACTGAAAGCGGa < 1:3745855/70‑1 (MQ=255)
ttGACGGCATAATCCAGAAAGCAAAAGCAGGATGCCCGGTCTCTCAAGTACTGAAAGCGGaaa > 1:4449837/1‑63 (MQ=255)
gACGGCATAATCCAGAAAGCAAAAGCAGGATGCCCGGTCTCTCAAGTACTGAAAGCGGAAATTACGCTgg < 1:936911/70‑1 (MQ=255)
gACGGCATAATCCAGAAAGCAAAAGCAGGATGCCCGGTCTCTCAAGTACTGAAAGCGGAAATTACGCTgg < 1:1717538/70‑1 (MQ=255)
gCATAATCCAGAAAGCAAAAGCAGGATGCCCGGTCTCTCAAGTACTGAAAG‑GGAAATTACGCTGGATTAc > 1:3392075/1‑70 (MQ=255)
cATAATCCAGAAAGCAAAAGCAGGATGCCCGGTCTCTCAAGTACTGAAAGCGGAAATTACGCTGGATTAc > 1:2297098/1‑70 (MQ=255)
aTAATCCAGAAAGCAAAAGCAGGATGCCCGGTCTCTCAAGTACTGAAAGCGGaaa > 1:347885/1‑55 (MQ=255)
aTAATCCAGAAAGCAAAAGCAGGATGCCCGGTCTCTCAAGTAATGAAAGCGGaaa > 1:3119644/1‑55 (MQ=255)
aTAATCCAGAAAGCAAAAGCAGGATGCCCGGTCTCTCAAGAACTGAAAGCGGAAATTACGCTGGATTAc > 1:995106/1‑69 (MQ=255)
tAATCCAGAAAGCAAAAGCAGGATGCCCGGTCTCTCAAGTACTGAAAGCGGAAATTACGCTGGATTAcc < 1:4295262/69‑1 (MQ=255)
aTCCAGAAAGCAAAAGCAGGATGCCCGGTCTCTCAAGTACTGAAAGCGGAAATTACGCTGGATTACCa > 1:4684806/1‑68 (MQ=255)
aTCCAGAAAGCAAAAGCAGGATGCCCGGTCTCTCAAGTACTGAAAGCGGAAATTACGCTGGATTACCAGtt > 1:2409474/1‑71 (MQ=255)
aTCCAGAAAGCAAAAGCAGGATGCCCGGTCTCTCAAGTACTGAAAGCGGAAATTACGCTGGATTACCAGtt > 1:3043520/1‑71 (MQ=255)
aTCCAGAAAGCAAAAGCAGGATGCCCGGTCTCTCAAGTACTGAAAGCGGAAATTACGCTGGATTACCAGtt > 1:1201143/1‑71 (MQ=255)
aTCCAGAAAGCAAAAGCAGGATGCCCGGTCTCTCAAGTACTGAAAGCGGAAATTACGCTGGATTACCAGtt > 1:1267026/1‑71 (MQ=255)
aTCCAGAAAGCAAAAGCAGGATGCCCGGTCTCTCAAGTACTGAAAGCGGAAATTACGCTGGATTACCAGtt > 1:899856/1‑71 (MQ=255)
aTCCAGAAAGCAAAAGCAGGATGCCCGGTCTCTCAAGTACTGAAAGCGGAAATTACGCTGGATTACCAGtt > 1:1838079/1‑71 (MQ=255)
cAGAAAGCAAAAGCAGGATGCCCGGTCTCTCAAGTACTGAAAGCGGAAATTACGCTGGATTACCAGTTGa > 1:2398882/1‑70 (MQ=255)
aaagcaaaagcaGGATGCCCGGTCTCTCAAGTACTGAAAGCGGAAATTACGCTGGATTACCAGTTGAAAt < 1:3736253/70‑1 (MQ=255)
agcaaaagcaGGATGCCCGGTCTCTCAAGTACTGAAAGCgg > 1:1631229/1‑41 (MQ=255)
gcaaaagcaGGATGCCCGGTCTCTCAAGTACTGAAAGCGGAAATTACGCTGGATTACCAGTTGAAATCGTa < 1:1094997/71‑1 (MQ=255)
aaaagcaGGATGCCCGGTCTCTCAAGTACTGAAAGCGGAAATTACGCTGGATTACCAGTTGAAATCGTaaa < 1:2499269/71‑1 (MQ=255)
aaagcaGGATGCCCGGTCTCTCAAGTACTGTAAGCGGAAATTACGCTGGATTACCAGTTGAAATCGTAAAg < 1:2201309/71‑1 (MQ=255)
aaagcaGGATGCCCGGTCTCTCAAGTACTGAAAGCGGAAATTACGCTGGATTACCAGTTGAAATCGTAAAg < 1:3159243/71‑1 (MQ=255)
aaagcaGGATGCCCGGTCTCTCAAGTACTGAAAGCGGAAATTACGCTGGATTACCAGTTGAAATCGTAAAg < 1:267738/71‑1 (MQ=255)
aaagcaGGATGCCCGGTCTCTCAAGTACTGAAAGCGGAAATTACGCTGGATTACCAGTTGAAATCGTAAAg < 1:1491440/71‑1 (MQ=255)
aaagcaGGATGCCCGGTCTCTCAAGTACTGAAAGCGGAAATTACGCTGGATTACCAGTTGAAATCGTAAAg < 1:2078168/71‑1 (MQ=255)
aagcaGGATGCCCGGTCTCTCAAGTACTGAAAGCGGAAATTACGCTGGATTACCAGTTGAAATCGTAAAGc < 1:313351/71‑1 (MQ=255)
aagcaGGATGCCCGGTCTCTCAAGTACTGAAAGCGGAAATTACGCTGGATTACCAGTTGAAATCGTAAAGc < 1:1274658/71‑1 (MQ=255)
aagcaGGATGCCCGGTCTCTCAAGTACTGAAAGCGGAAATTACGCTGGATTACCAGTTGAAATCGTAAAGc < 1:1777329/71‑1 (MQ=255)
aagcaGGATGCCCGGTCTCTCAAGTACTGAAAGCGGAAATTACGCTGGATTACCAGTTGAAATCGTAAAGc < 1:1854448/71‑1 (MQ=255)
aagcaGGATGCCCGGTCTCTCAAGTACTGAAAGCGGAAATTACGCTGGATTACCAGTTGAAATCGTAAAGc < 1:3739965/71‑1 (MQ=255)
agcaGGATGCCCGGTCTCTCAAGTACTGAAAGCGGAAATTACGCTGGATTACCAGTTGAAATCGTAAAGc > 1:106644/1‑70 (MQ=255)
gATGCCCGGTCTCTCAAGTACTGAAAGCGGAAATTACGCTGGATTACCAGTTGAAATCGTAAAGc < 1:3629840/65‑1 (MQ=255)
gATGCCCGGTCTCTCAAGTACTGAAAGCGGAAATTACGCTGGATTACCAGTTGAAATCGTAAAGc < 1:4031085/65‑1 (MQ=255)
aTGCCCGGTCTCTCAAGTACTGAAAGCGGAAATTACGCTGGATTACCAGTTGAAATCGTAAAg < 1:3698931/63‑1 (MQ=255)
aTGCCCGGTCTCTCAAGTACTGAAAGCGGAAATTACGCTGGATTACCAGTTGAAATCGTAAAGCATTGCCg < 1:3524455/71‑1 (MQ=255)
aTGCCCGGTCTCTCAAGTACTGAAAGCGGAAATTACGCTGGATTACCAGTTGAAATCGTAAAGCATTGCCg > 1:1925349/1‑71 (MQ=255)
cccGGTCTCTCAAGTACTGAAAGCGGAAATTACGCTGGATTACCAGTTGAAATCGTAAAGCATTGCCGGAt < 1:2125910/71‑1 (MQ=255)
gTCTCTCAAGTACTGAAAGCGGAAATTACGCTGGATTACCAGTTGAAATCGTAAAGc < 1:3292651/57‑1 (MQ=255)
ctctcAAGTACTGAAAGCGGAAATTACGCTGGATTACCAGTTGAAATCGTAAAGCATTGCCGGATGAcgcg > 1:2288871/1‑71 (MQ=255)
ctctcAAGTACTGAAAGCGGAAATTACGCTGGATTACCAGTTGAAATCGTAAAGCATTGCCGGATGAcgcg > 1:1846102/1‑71 (MQ=255)
ctctcAAGTACTGAAAGCGGAAATTACGCTGGATTACCAGTTGAAATCGTAAAGCATTGCCGGATGAcgcg > 1:2302638/1‑71 (MQ=255)
ctctcAAGTACTGAAAGCGGAAATTACGCTGGATTACCAGTTGAAATCGTAAAGCATTGCCGGATGAcgcg > 1:2614091/1‑71 (MQ=255)
ctctcAAGTACTGAAAGCGGAAATTACGCTGGATTACCAGTTGAAATCGTAAAGCATTGCCGGATGAcgcg > 1:3107951/1‑71 (MQ=255)
tctcAAGTACTGAAAGCGGAAATTACGCTGGATTACCAGTTGAAATCGTAAAGCa > 1:4343352/1‑55 (MQ=255)
tctcAAGTACTGAAAGCGGAAATTACGCTGGATTACCAGTTGAAATCGTAAAGCATTGCCGGATGACGCGt > 1:4121149/1‑71 (MQ=255)
|
CCGGGTATTGATGCCTCTACCTTTGACGGCATAATCCAGAAAGCAAAAGCAGGATGCCCGGTCTCTCAGGTACTGAAAGCGGAAATTACGCTGGATTACCAGTTGAAATCGTAAAGCATTGCCGGATGACGCGT > W3110S.gb/1558657‑1558790
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
