Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F28 I1 R2
|
328 |
63.0 |
4687567 |
98.0% |
4593815 |
65.2 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
1,832,108 |
C→T |
T124T (ACG→ACA) |
astA ← |
arginine succinyltransferase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 1,832,108 | 0 | C | T | 100.0%
| 145.6
/ NA
| 50 | T124T (ACG→ACA) | astA | arginine succinyltransferase |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (19/31); total (19/31) |
AACATAAAGCGCGATTTCGACAGCAAATAGCCGTTGCCCTCTTTGCGCCAGTCCGGGTCGAGAAACAGCGTGCACAGCTCGCTGCTGCCGGTGTGATCGTTACTGAGAAACAGCGTCGGCAATGCGTTATAGA > W3110S.gb/1832040‑1832172
|
aacataaaGCGCGATTTCGAGAGCAAATAGCCGTTGCCCTCTTTGCGCCAGTCCGGGTCGAGAAACAgtgt > 1:1652578/1‑71 (MQ=255)
aacataaaGCGCGATTTCGACAGCAAATAGCCGTTGCCCTCTTTGCGCCAGTCCTGGTCGAGAAACAgtgt > 1:2612846/1‑71 (MQ=255)
aacataaaGCGCGATTTCGACAGCAAATAGCCGTTGCCCTCTTTGCGCCAGTCCGGGTCGAGAAACAgtgt > 1:3740203/1‑71 (MQ=255)
aacataaaGCGCGATTTCGACAGCAAATAGCCGTTGCCCTCTTTGCGCCAGTCCGGGTCGAGAAACAgtgt > 1:2745998/1‑71 (MQ=255)
aacataaaGCGCGATTTCGACAGCAAATAGCCGTTGCCCTCTTTGCGCCAGTCCGGGTCGAGAAACAgtgt > 1:4147610/1‑71 (MQ=255)
aacataaaGCGCGATTTCGACAGCAAATAGCCGTTGCCCTCTTTGCGCCAGTCCGGGTCGAGAAACAgtgt > 1:4390773/1‑71 (MQ=255)
aacataaaGCGCGATTTCGACAGCAAATAGCCGTTGCCCTCTTTGCGCCAGTCCGGGTCGAGAAACAgtgt > 1:1855112/1‑71 (MQ=255)
aacataaaGCGCGATTTCGACAGCAAATAGCCGTTGCCCTCTTTGCGCCAGTCCGGGTCGAGAAACAgtgt > 1:4094256/1‑71 (MQ=255)
aacataaaGCGCGATTTCGACAGCAAATAGCCGTTGCCCTCTTTGCGCCAGTCCGGGTCGAGAAACAgtgt > 1:4575798/1‑71 (MQ=255)
aacataaaGCGCGATTTCGACAGCAAATAGCCGTTGCCCTCTTTGCGCCAGTCCGGGTCGAGAAACAgtgt > 1:1734202/1‑71 (MQ=255)
aacataaaGCGCGATTTCGACAGCAAATAGCCGTTGCCCTCTTTGCGCCAGTCCGGGTCGAGAAACAgtgt > 1:1498006/1‑71 (MQ=255)
aacataaaGCGCGATTTCGACAGCAAATAGCCGTTGCCCTCTTTGCGCCAGTCCGGGTCGAGAAACAgtgt > 1:148506/1‑71 (MQ=255)
acataaaGCGCGATTTCGACAGCAAATAGCCGTTGCCCTCTTTGCGCCAGTCCGGGTCGAGAAACAGtgtg < 1:645339/71‑1 (MQ=255)
taaaGCGCGATTTCGACAGCAAATAGCCGTTGCCCTCTTTGCGCCAGTCCGGGTCGAGAAACAGTGTGcac < 1:3093760/71‑1 (MQ=255)
aaaGCGCGATTTCGACAGCAAATAGCCGTTGCCCTCTTTGCGCCAGTCCGGGTCGAGAAACAGTGTGca > 1:3848557/1‑69 (MQ=255)
aaaGCGCGATTTCGACAGCAAATAGCCGTTGCCCTCTTTGCGCCAGTCCGGGTCGAGAAACAGTGTGca > 1:2761609/1‑69 (MQ=255)
gATTTCGACAGCAAATAGCCGTTGCCCTCTTTGCGCCAGTCCGGGTCGAGAAACAGTGTGCACAGCTCgct < 1:3966482/71‑1 (MQ=255)
gATTTCGACAGCAAATAGCCGTTGCCCTCTTTGCGCCAGTCCGGGTCGAGAAACAGTGTGCACAGCTCgct < 1:3133522/71‑1 (MQ=255)
gATTTCGACAGCAAATAGCCGTTGCCCTCTTTGCGCCAGTCCGGGTCGAGAAACAGTGTGCACAGCTCgct < 1:3571343/71‑1 (MQ=255)
ttagACAGCAAATAGCCGTTGCCCTCTTTGCGCCAGTCCGGGTCGAGACACAGTGTGCACAGCTCgctgct > 1:15127/4‑71 (MQ=255)
ttagACAGCAAATAGCCGTTGCCCTCTTTGCGCCAGTCCGGGTCGAGAAACAGTGTGCACAGCTCgctgct > 1:3060412/4‑71 (MQ=255)
ttCGACAGCAAATAGCCGTTGCCCTCTTTGCGCCAGTCCGGGTCGAGAAACAGTGTGCACAGCTCgctgc > 1:3345296/1‑70 (MQ=255)
tCGACAGCAAATAGCCGTTGCCCTCTTTGCGCCAGTCCGGGTCGAGAAACAGTGTGCACAGCTCGctgctg > 1:4646575/1‑71 (MQ=255)
cGACAGCAAATAGCCGTTGCCCTCTTTGCGCCAGTCCGGGTCGAGAAACAGTGTGCACAGCTCgctgct < 1:1033205/69‑1 (MQ=255)
aaaTAGCCGTTGCCCTCTTTGCGCCAGTCCGGGTCGAGAAACAGTGTGCACAGCTCGCTGCTGCCGGtgtg < 1:3019784/71‑1 (MQ=255)
aaaTAGCCGTTGCCCTCTTTGCGCCAGTCCGGGTCGAGAAACAGTGTGCACAGCTCGCTGCTGCCGGtgtg < 1:2380358/71‑1 (MQ=255)
aaaTAGCCGTTGCCCTCTTTGCGCCAGTCCGGGTCGAGAAACAGTGTGCACAGCTCGCTGCTGCCGGtgtg < 1:4422888/71‑1 (MQ=255)
aaaTAGCCGTTGCCCTCTTTGCGCCAGTCCGGGTCGAGAAACAGTGTGCACAGCTCGCTGCTGCCGGtgtg < 1:3843079/71‑1 (MQ=255)
tAGCCGTTGCCCTCTTTGCGCCAGTCCGGGTCGAGAAACAGTGTGCACAGCTCGCTGCTGCCGGTGTGATc > 1:442951/1‑71 (MQ=255)
tAGCCGTTGCCCTCTTTGCGCCAGTCCGGGTCGAGAAACAGTGTGCACAGCTCGCTGCTGCCGGTGTGATc > 1:99190/1‑71 (MQ=255)
tAGCCGTTGCCCTCTTTGCGCCAGTCCGGGTCGAGAAACAGTGTGCACAGCTCGCTGCTGCCGGTGTGATc > 1:3348008/1‑71 (MQ=255)
tAGCCGTTGCCCTCTTTGCGCCAGTCCGGGTCGAGAAACAGTGTGCACAGCTCGCTGCTGCCGGTGTGATc > 1:2256208/1‑71 (MQ=255)
aGCCGTTGCCCTCTTTGCGCCAGTCCGGGTCGAGAAACAGTGTGCACAGCTCGCTGCTGCCGGTGTGATCg > 1:4339221/1‑71 (MQ=255)
gCCGTTGCCCTCTTTGCGCCAGTCCGGGTCGAGAAACAGTGTGCACAGCTCGCTGCTTCCGGTGTGATCGt < 1:127007/71‑1 (MQ=255)
gCCGTTGCCCTCTTTGCGCCAGTCCGGGTCGAGAAACAGTGTGCACAGCTCGCTGCTGCCGGTGTGATCGt < 1:701034/71‑1 (MQ=255)
gCCGTTGCCCTCTTTGCGCCAGTCCGGGTCGAGAAACAGTGTGCACAGCTCGCTGCTGCCGGTGTGATCGt < 1:4030902/71‑1 (MQ=255)
gCCGTTGCCCTCTTTGCGCCAGTCCGGGTCGAGAAACAGTGTGCACAGCTCGCTGCTGCCGGTGTGATCGt < 1:1786277/71‑1 (MQ=255)
gCCGTTGCCCTCTTTGCGCCAGTCCGGGTCGAGAAACAGTGTGCACAGCTCGCTGCTGCCGGTGTGATCGt < 1:4036657/71‑1 (MQ=255)
gCCGTTGCCCTCTTTGCGCCAGTCCGGGTCGAGAAACAGTGTGCACAGCTCGCTGCTGCCGGTGTGATCGt < 1:3198789/71‑1 (MQ=255)
gCCGTTGCCCTCTTTGCGCCAGTCCGGGTCGAGAAACAGTGTGCACAGCTCGCTGCTGCCGGTGTGATCGt < 1:4383260/71‑1 (MQ=255)
gCCGTTGCCCTCTTTGCGCCAGTCCGGGTCGAGAAACAGTGTGCACAGCTCGCTGCTGCCGGTGTGATCGt < 1:4172882/71‑1 (MQ=255)
ccGTTGCCCTCTTTGCGCCAGTCCGGGTCGAGAAACAGTGTGCACAGCTCGCTGCTGCCGGTGTGATCGtt < 1:926233/71‑1 (MQ=255)
ccGTTGCCCTCTTTGCGCCAGTCCGGGTCGAGAAACAGTGTGCACAGCTCGCTGCTGCCGGTGTGATCGtt < 1:328785/71‑1 (MQ=255)
ccGTTGCCCTCTTTGCGCCAGTCCGGGTCGAGAAACAGTGTGCACAGCTCGCTGCTGCCGGTGTGATCGtt < 1:2266715/71‑1 (MQ=255)
gCCCTCTTTGCGCCAGTCCGGGTCGAGAAACAGTGTGCACAGCTCGCTGCTGCCGGTGTGAt > 1:3499093/1‑62 (MQ=255)
ccTCTTTGCGCCAGTCCGGGTCGAGAAACAGTGTGCACAGCTCGCTGCTGCCGGTGTGATCGTTACTgaga < 1:635571/71‑1 (MQ=255)
ctctTTGCGCCAGTCCGGGTCGAGAAACAGTGTGCACAGCTCGCTGCTGCCGGTGTGATCGtt < 1:952727/63‑1 (MQ=255)
ctctTTGCGCCAGTCCGGGTCGAGAAACAGTGTGCACAGCTCGCTGCTGCCGGTGTGATCGTTACTgaga > 1:4214156/1‑70 (MQ=255)
ctctTTGCGCCAGTCCGGGTCGAGAAACAGTGTGCACAGCTCGCTGCTGCCGGTGTCATCGtt < 1:1810179/63‑1 (MQ=255)
tctTTGCGCCAGTCCGGGTCGAGAAACAGTGTGCACAGCTc < 1:2736423/41‑1 (MQ=255)
tctTTGCGCCAGTCCGGGTCGAGAAACAGTGTGCACAGCTCGCTGCTGCCGGTGTGATCGt < 1:4613346/61‑1 (MQ=255)
tttGCGCCAGTCCGGGTCGAGAAACAGTGTGCACAGCTCGCTGCTGCCGGTGTGATCGTTACTGAGaaa > 1:4544769/1‑69 (MQ=255)
tGCGCCAGTCCGGGTCGAGAAACAGTGTGCACAGCTCGCTGCTGCCGGTGTGATCGTTACTGAGAAACAGc > 1:3610692/1‑71 (MQ=255)
tGCGCCAGTCCGGGTCGAGAAACAGTGTGCACAGCTCGCTGCTGCCGGTGTGATCGTTACTGAGAAACAGc > 1:4139328/1‑71 (MQ=255)
cAGTCCGGGTAGAGAAACAGTGTGCACAGCTCGCTGCTGCCGGTGTGATCGTTAc > 1:4259934/1‑55 (MQ=255)
aGTCCGGGTCGAGAAACAGTGTGCACAGCTCGCTGCTGCCGGTGTGATCGTTa > 1:3478817/1‑53 (MQ=255)
aGTCCGGGTCGAGAAACAGTGTGCACAGCTCGCTGCTGCCGGTGTGATCGTTACTGAGAAACAGCGTCGGc < 1:1270420/71‑1 (MQ=255)
aGTCCGGGTCGAGAAACAGTGTGCACAGCTCGCTGCTGCCGGTGTGATCGTTACTGAGAAACAGCGTCGGc < 1:3805406/71‑1 (MQ=255)
ggTCGAGAAACAGTGTGCACAGCTCGCTGCTGCCGGTGTGATCGTTACTGAGAAACAGCGTCGGCAATGCg < 1:842572/71‑1 (MQ=255)
gTCGAGAAACAGTGTGCACAGCTCGCTGCTGCCGGTGTGATCGTTACTTAGAAACAGCGTCGGCAATGCGt < 1:4121295/71‑1 (MQ=255)
gTCGAGAAACAGTGTGCACAGCTCGCTGCTGCCGGTGTGATCGTTACTGAGAAACAGCGTCGGCAATGCGt < 1:457593/71‑1 (MQ=255)
tCGAGAAACAGTGTGCACAGCTCGCTGCTGCCGGTGTGATCGTTACTGAGAAACAGCGTCGGCAATGCGt > 1:4032972/1‑70 (MQ=255)
aaaCAGTGTGCACAGCTCGCTGCTGCCGGTGTGATCGTTACTGAGAAACAGCGTCGGCAATGCGTTATAGa < 1:1129093/71‑1 (MQ=255)
|
AACATAAAGCGCGATTTCGACAGCAAATAGCCGTTGCCCTCTTTGCGCCAGTCCGGGTCGAGAAACAGCGTGCACAGCTCGCTGCTGCCGGTGTGATCGTTACTGAGAAACAGCGTCGGCAATGCGTTATAGA > W3110S.gb/1832040‑1832172
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
