Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F28 I1 R2
|
328 |
63.0 |
4687567 |
98.0% |
4593815 |
65.2 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
1,838,873 |
C→T |
P363S (CCG→TCG) |
ynjB → |
conserved hypothetical protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 1,838,873 | 0 | C | T | 100.0%
| 101.3
/ NA
| 36 | P363S (CCG→TCG) | ynjB | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (8/28); total (8/28) |
GATCCGCAAAAACTGCCTGACGGGCAGCGCGAATCATTGCAATCAAGAATGCCGCAGGATCTGCCGCCGGTACTGGCTGAACCGCACGCAGGTTGGGTAAATGCGCTGGAACAAGAATGGC > W3110S.gb/1838810‑1838930
|
gATCCGCAAAAACTGCCTGACGGGCAGCTCGAATCATTGCAATCAAGAATGCCGCAGGATCTGTCGCCgg < 1:2285006/70‑1 (MQ=255)
gATCCGCAAAAACTGCCTGACGGGCAGCGCGAATCATTGCAATCAAGAATGCCGCAGGATCTGTCGCCgg < 1:4510790/70‑1 (MQ=255)
gATCCGCAAAAACTGCCTGACGGGCAGCGCGAATCATTGCAATCAAGAATGCCGCAGGATCTGTCGCCgg < 1:173279/70‑1 (MQ=255)
gATCCGCAAAAACTGCCTGACGGGCAGCGCGAATCATTGCAATCAAGAATGCCGCAGGATCTGTCGCCgg < 1:3827453/70‑1 (MQ=255)
gATCCGCAAAAACTGCCTGACGGGCAGCGCGAATCATTGCAATCAAGAATGCCGCAGGATCTGTCGCCgg < 1:3219537/70‑1 (MQ=255)
gATCCGCAAAAACTGCCTGACGGGCAGCGCGAATCATTGCAATCAAGAATGCCGCAGGATCTGTCGCCgg < 1:2345601/70‑1 (MQ=255)
gATCCGCAAAAACTGCCTGACGGGCAGCGCGAATCATTGCAATCAAGAATGCCGCAGGATCTGTCGCCgg < 1:309735/70‑1 (MQ=255)
gATCCGCAAAAACTGCCTGACGGGCAGCGCGAATCATTGCAATCAAGAATGCCGCAGGATCTGTCGCCGGt < 1:470523/71‑1 (MQ=255)
aaaaCTGCCTGACGGGCAGCGCGAATCATTGCAATCAAGAATGCCGCAGGATCTGTCGCCGGTAc > 1:4511503/1‑65 (MQ=255)
aaaaCTGCCTGACGGGCAGCGCGAATCATTGCAATCAAGAATGCCGCAGGATCTGTCGCCGGTAc > 1:2345803/1‑65 (MQ=255)
aaaaCTGCCTGACGGGCAGCGCGAATCATTGCAATCAAGAATGCCGCAGGATCTGTCGCCGGTACTGGCt > 1:2335584/1‑70 (MQ=255)
aaaCTGCCTGACGGGCAGCGCGAATCATTGCAATCAAGAATCCCGCAGGATCTGTCGCCGGTACTGGCTGa < 1:3109716/71‑1 (MQ=255)
gCCTGACGGGCAGCGCGAATCATTGCAATCAAGAATGCCGCAGGATCTGTCGCCGGt < 1:3041291/57‑1 (MQ=255)
gCCTGACGGGCAGCGCGAATCATTGCAATCAAGAATGCCGCAGGATCTGTCGCCGGTACTGGCTGAACcg > 1:655931/1‑70 (MQ=255)
aCGGGCAGCGCGAATCATTGCAATCAAGAATGCCGCAGGATCTGTCGCCgg < 1:2159624/51‑1 (MQ=255)
gggCAGCGCGAATCATTGCAATCAAGAATGCCGCAGGATCTGTCGCCGGTACTGGCTGAACCGCACGCAgg > 1:1129084/1‑71 (MQ=255)
gcgAATCATTGCAATCAAGAATGCCGCAGGATCTGTCGCCGGTACTGGCTGAACCGCACGCAGGTTGGGTa < 1:666350/71‑1 (MQ=255)
gcgAATCATTGCAATCAAGAATGCCGCAGGATCTGTCGCCGGTACTGGCTGAACCGCACGCAGGTTGGGTa < 1:353980/71‑1 (MQ=255)
gcgAATCATTGCAATCAAGAATGCCGCAGGATCTGTCGCCGGTACTGGCTGAACCGCACGCAGGTTGGGTa < 1:4206837/71‑1 (MQ=255)
cgAATCATTGCAATCAAGAATGCCGCAGGATCTGTCGCCGGt < 1:2174915/42‑1 (MQ=255)
aTTGCAATCAAGAATGCCGCAGGATCTGTCGCCGGTACTGGCTGAACCGCACGCAg < 1:3699199/56‑1 (MQ=255)
ttGCAATCAAGAATGCCGCAGGATCTGTCGCCGGTACTGGCTGAACcgcacgca < 1:4342944/54‑1 (MQ=255)
tCAAGAATGCCGCAGGATCTGTCGCCGGTACTGGCTGAACCGCACGCAGGTTGGGTAAATGCGCTGGAACa < 1:4340540/71‑1 (MQ=255)
cAAGAATGCCGCAGGATCTGTCGCCGGTACTGGCTGAACCGCACGCAGGTTGGGTAAATGCGCTGGAAc > 1:3383232/1‑69 (MQ=255)
aaGAATGCCGCAGGATCTGTCGCCGGTACTGGCTGAACCGCACGCAGGTTGGGTAAATGCGCTGGAACAAg < 1:1406547/71‑1 (MQ=255)
aaGAATGCCGCAGGATCTGTCGCCGGTACTGGCTGAACCGCACGCAGGTTGGGTAAATGCGCTGGAACAAg < 1:541307/71‑1 (MQ=255)
aaGAATGCCGCAGGATCTGTCGCCGGTACTGGCTGAACCGCACGCAGGTTGGGTAAATGCGCTGGAACAAg < 1:52522/71‑1 (MQ=255)
aaGAATGCCGCAGGATCTGTCGCCGGTACTGGCTGAACCGCACGCAGGTTGGGTAAATGCGCTGGAACAAg < 1:973055/71‑1 (MQ=255)
aaGAATGCCGCAGGATCTGTCGCCGGTACTGGCTGAACCGCACGCAGGTTGGGTAAATGCGCTGGAACAAg < 1:3655769/71‑1 (MQ=255)
aaGAATGCCGCAGGATCTGTCGCCGGTACTGGCTGAACCGCACGCAGGTTGGGTAAATGCGCTGGAACAAg < 1:3147932/71‑1 (MQ=255)
aaGAATGCCGCAGGATCTGTCGCCGGTACTGGCTGAACCGCACGCAGGTTGGGTAAATGCGCTGGAACAAg < 1:2254587/71‑1 (MQ=255)
aaGAATGCCGCAGGATCTGTCGCCGGTACTGGCTGAACCGCACGCAGGTTGGGTAAATGCGCTGGAACAAg < 1:1587345/71‑1 (MQ=255)
aaGAATGCCGAAGGATCTGTCGCCGGTACTGGCTGAACCGCAAGCAGGTTGGGTAAATGCGCTGGAAc > 1:604996/1‑68 (MQ=255)
tGCCGCAGGATCTGTCGCCGGTACTGGCTGAACCGCACGCAGGTTGGGTAAATGCGCTGGAACAAGAAt < 1:2383628/69‑1 (MQ=255)
ccGCAGGATCTGTCGCCGGTACTGGCTGAACCGCACGCAGGTTGGGTAAATGCGCTGGAACAAGAATGGc < 1:787443/70‑1 (MQ=255)
cTGTCGCCGGTACTGGCTGAACCGCACGCAGGTTGGGTAAATgcgc > 1:3455581/1‑46 (MQ=255)
|
GATCCGCAAAAACTGCCTGACGGGCAGCGCGAATCATTGCAATCAAGAATGCCGCAGGATCTGCCGCCGGTACTGGCTGAACCGCACGCAGGTTGGGTAAATGCGCTGGAACAAGAATGGC > W3110S.gb/1838810‑1838930
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
