Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F28 I1 R2
|
328 |
63.0 |
4687567 |
98.0% |
4593815 |
65.2 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
1,935,986 |
C→T |
M111I (ATG→ATA) |
edd ← |
6‑phosphogluconate dehydratase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 1,935,986 | 0 | C | T | 100.0%
| 184.7
/ NA
| 61 | M111I (ATG→ATA) | edd | 6‑phosphogluconate dehydratase |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (42/19); total (42/19) |
ATCGCTATCACTTCGCGGCTTAGCAGCGACAATTCCATTCCATCCTGCCCCTGGGTGACACCATCACACATCGCCGGAACACCGCCCGCAACCTGACCAACCGCATTCGCTTCATGCAGGGCTTTACGAATGATTT > W3110S.gb/1935918‑1936053
|
aTCGCTATCACTTCGCGGCTTAGCAGCGACAATTCCATTCCATCCTGCCCCTGGGTGACACCATCACatat > 1:2954337/1‑71 (MQ=255)
aTCGCTATCACTTCGCGGCTTAGCAGCGACAATTCCATTCCATCCTGCCCCTGGGTGACACCATCACatat > 1:1514672/1‑71 (MQ=255)
aTCGCTATCACTTCGCGGCTTAGCAGCGACAATTCCATTCCATCCTGCCCCTGGGTGACACCATCACatat > 1:3981999/1‑71 (MQ=255)
aTCGCTATCACTTCGCGGCTTAGCAGCGACAATTCCATTCCATCCTGCCCCTGGGTGACACCATCACatat > 1:1869892/1‑71 (MQ=255)
aTCGCTATAACTTCGCGGCTTAGCAGCGACAATTCCATTCCATCATGCCCCTGGGTGACACCATCACatat > 1:1337308/1‑71 (MQ=255)
gCTATCACTTCGCGGCTTAGCAGCGACAATTCCATTCCATCCTGCCCCTGGGTGACACCATCACATATCGc > 1:994839/1‑71 (MQ=255)
gCTATCACTTCGCGGCTTAGCAGCGACAATTCCATTCCATCCTGCCCCTGGGTGACACCATCACATATCGc > 1:4364383/1‑71 (MQ=255)
gCTATCACTTCGCGGCTTAGCAGCGACAATTCCATTCCATCCTGCCCCTGGGTGACACCATCACATATCGc > 1:4614747/1‑71 (MQ=255)
gCTATCACTTCGCGGCTTAGCAGCGACAATTCCATTCCATCCTGCCCCTGGGTGACACCATCACATATCGc > 1:4624928/1‑71 (MQ=255)
gCTATCACTTCGCGGCTTAGCAGCGACAATTCCATTCCATCCTGCCCCTGGGTGACACCATCACATATCGc > 1:550558/1‑71 (MQ=255)
gCTATCACTTCGCGGCTTAGCAGCGACAATTCCATTCCATCCTGCCCCTGGGTGACACCATCACATATCGc > 1:1781860/1‑71 (MQ=255)
tCACTTCGCGGCTTAGCAGCGACAATTCCATTCCATCCTGCCCCTGGGTGACACCATCACATATCGCCgg < 1:321761/70‑1 (MQ=255)
aCTTCGCGGCTTAGCAGCGACAATTCCATTCCATCCTGCCCCTGGGTGACACCATCACATATCGCCGGaa > 1:3253129/1‑70 (MQ=255)
aCTTCGCGGCTTAGCAGCGACAATTCCATTCCATCCTGCCCCTGGGTGACACCATCACATATCGCCGGaa > 1:2600818/1‑70 (MQ=255)
tCGCGGCTTAGCAGCGACAATTCCATTCCATCCTGCCCCTGGGTGACACCATCACATATCGCCGGAACAcc > 1:1406967/1‑71 (MQ=255)
cTTAGCAGCGACAATTCCATTCCATCCTGCCCCTGGGTGACACCATCACATATCGCCGGAACAccgcccgc < 1:4227057/71‑1 (MQ=255)
agcagcGACAATTCCATTCCATCCTGCCCCTGGGTGACACCATCACATATCGCCGGAACACCGCCCGCa < 1:3069412/69‑1 (MQ=255)
agcagcGACAATTCCATTCCATCCTGCCCCTGGGTGACACCATCACATATCGCCGGAACACCGCCCGCa < 1:3293108/69‑1 (MQ=255)
aaTTCCATTCCATCCTGCCCCTGGGTGACACCATCACATATCGCCGGAACACCGCCCGCAACCTGACCAAc > 1:1557317/1‑71 (MQ=255)
aaTTCCATTCCATCCTGCCCCTGGGTGACACCATAACATATCGCCGGAACACCGCCCGCAACCTGACCAAc > 1:485061/1‑71 (MQ=255)
ttccattccaTCCTGCCCCTGGGTGACACCATCACATATCGCCGGAACACCGCCCGCAACCTGACCAACCg < 1:3408597/71‑1 (MQ=255)
tccattccatCCTGCCCCTGGGTGACACCATCACATATCGCCGGAACACCGCCCGCAACCTGACCAACCg > 1:3150925/1‑70 (MQ=255)
tccattccatCCTGCCCCTGGGTGACACCATCACATATCGCCGGAACACCGCCCGCAACCTGACCAACCg > 1:1908057/1‑70 (MQ=255)
ttccatCCTGCCCCTGGGTGACACCATCACATATCGCCGGAACACCGCCCGCAACCTg < 1:650373/58‑1 (MQ=255)
tccatCCTGCCCCTGGGTGACACCATCACATATCGCCGGAACACCGCCCGCAACCTGACCAACCGCATTCg > 1:2421472/1‑71 (MQ=255)
ccatCCTGCCCCTGGGTGACACCATCACATATCGCCGGAACACCGCCCGCAACCTGa > 1:1802176/1‑57 (MQ=255)
ccatCCTGCCCCTGGGTGACACCATCACATATCGCCGGAACACCGCCCGCAACCTGACCAACCGCATTCg < 1:1490276/70‑1 (MQ=255)
ccatCCTGCCCCTGGGTGACACCATCACATATCGCCGGAACACCGCCCGCAACCTGACCAACCGCATTCg < 1:3164569/70‑1 (MQ=255)
ccatCCTGCCCCTGGGTGACACCATCACATATCGCCGGAACACCGCCCGCAACCTGACCAACCGCATTCGc > 1:1227348/1‑71 (MQ=255)
ccatCCTGCCCCTGGGTGACACCATCACATATCGCCGGAACACCGCCCGCAACCTGACCAACCGCATTCGc > 1:550387/1‑71 (MQ=255)
ccatCCTGCCCCTGGGTGACACCATCACATATCGCCGGAACACCGCCCGCAACCTGACCAACCGCATTCGc > 1:903776/1‑71 (MQ=255)
atCCTGACCCTGGGTGACACCATCACATATCGCCGGAACACCGCCCGCAACCTGACCAACCGCATTCGCtt > 1:1720181/1‑71 (MQ=255)
cTGCCCCTGGGTGACACCATCACATATCGCCGGAACACCGCCCGCAACCTGACCAACCGCATTCGCTTCAt > 1:2290975/1‑71 (MQ=255)
cTGCCCCTGGGTGACACCATCACATATCGCCGGAACACCGCCCGCAACCTGACCAACCGCATTCGCTTCAt > 1:4517076/1‑71 (MQ=255)
cTGCCCCTGGGTGACACCATCACATATCGCCGGAACACCGCCCGCAACCTGACCAACCGCATTCGCTTCAt > 1:2841852/1‑71 (MQ=255)
cTGCCCCTGGGTGACACCATCACATATCGCCGGAACACCGCCCGCAACCTGACCAACCGCATTCGCTTCAt > 1:976624/1‑71 (MQ=255)
cccccTGGGTGACACCATCACATATCGCCGGAACACCGCCCGCAACCTGACCAACCGCATTCGCTTCATg < 1:1550310/69‑1 (MQ=255)
ccccTGGGTGACACCATCACATATCGCCGGAACACCGCCCGCAACCTGACCAACCGCATTCGCTTCATg < 1:2304198/69‑1 (MQ=255)
ccccTGGGTGACACCATCACATATCGCCGGAACACCGCCCGCAACCTGACCAACCGCATTCGCTTCATg < 1:3962262/69‑1 (MQ=255)
ccTGGGTGACACCATCACATATCGCCGGAACACCGCCCGCAACCTGACCAACCGCATTCGCTTCATGCAgg < 1:3118529/71‑1 (MQ=255)
cTGGGTGACACCATCACATATCGCCGGAACACCGCCCGCAACCTGACCa < 1:1583241/49‑1 (MQ=255)
gggTGACACCATCACATATCGCCGGAACACCGCCCGCAACCTGACCAACCGCATTCGCTTCATGCAGGGCt > 1:1276666/1‑71 (MQ=255)
gggTGACACCATCACATATCGCCGGAACACCGCCCGCAACCTGACCAACCGCATTCGCTTCATGCAGGGCt > 1:3630264/1‑71 (MQ=255)
gggTGACACCATCACATATCGCCGGAACACCGCCCGCAACCTGACCAACCGCATTCGCTTCATGCAGGGCt > 1:4404614/1‑71 (MQ=255)
gggTGACACCATCACATATCGCCGGAACACCGCCCGCAACCTGACCAACCGCATTCGCTTCATGCAGGGCt > 1:3011122/1‑71 (MQ=255)
gggTGACACCATCACATATCGCCGGAACACCGCCCGCAACCTGACCAACCGCATTCGCTTCATGCAGGGCt > 1:1768010/1‑71 (MQ=255)
ggTGACACCATCACATATCGCCGGAACACCGCCCGCAACCTGAc > 1:1358875/1‑44 (MQ=255)
ggTGACACCATCACATATCGCCGGAACACCGCCCGCAACCTGAc > 1:3517534/1‑44 (MQ=255)
ggTGACACCATCACATATCGCCGGAACACCGCCCGCAACCTGACCAACCGCATTCGCTTCATGCAGGGc < 1:2111479/69‑1 (MQ=255)
acacCATCACATATCGCCGGAACACCGCCCGCAACCTGACCAACCGCATTCGCTTCATGCAGGGCTTTAc > 1:2973912/1‑70 (MQ=255)
acCATCACATATCGCCGGAACACCGGCCGCAACCTGACCAACCGCATTCGCTTCATGCAGGGCTTTACGaa > 1:2260969/1‑71 (MQ=255)
acCATCACATATCGCCGGAACACCGCCCGCAACCTGACCAACCGCATTCGCTTCATGCAGGGCTTTACGaa > 1:3575943/1‑71 (MQ=255)
acCATCACATATCGCCGGAACACCGCCCGCAACCTGACCAACCGCATTCGCTTCATGCAGGGCTTTACGaa > 1:2150150/1‑71 (MQ=255)
ccATCACATATCGCCGGAACACCGCCCGCAACCTGa > 1:1629359/1‑36 (MQ=255)
ccATCACATATCGCCGGAACACCGCCCGCAACCTGACCAACCGCATTCGCTTCATGCAGGGCTTTACGAAt < 1:95693/71‑1 (MQ=255)
ccATCACATATCGCCGGAACACCGCCCGCAACCTGACCAACCGCATTCGCTTCATGCAGGGCTTTACGAAt < 1:4133072/71‑1 (MQ=255)
ccATCACATATCGCCGGAACACCGCCCGCAACCTGACCAACCGCATTCGCTTCATGCAGGGCTTTACGAAt < 1:206330/71‑1 (MQ=255)
aTCACATATCGCCGGAACACCGCCCGCAACCTGACCAACCGCAtt > 1:2855842/1‑45 (MQ=255)
aTCACATATCGCCGGAACACCGCCCGCAACCTGACCAACCGCATTCGCTTCATGCAGGGCTTTACGAATg > 1:4480394/1‑70 (MQ=255)
aTCACATATCGCCGGAACACCGCCCGCAACCTGACCAACCGCATTCGCTTCATGCAGGGCTTTACGAATGa > 1:2583946/1‑71 (MQ=255)
aTCACATATCGCCGGAACACCGCCCGCAACCTGACCAACCGCATTCGCTTCATGCAGGGCTTTACGAATGa > 1:2956854/1‑71 (MQ=255)
aTCACATATCGCCGGAACACCGCCCGCAACCTGACCAACCGCATTCGCTTCATGCAGGGCTTTACGAATGa > 1:3685181/1‑71 (MQ=255)
aTCACATATCGCCGGAACACCGCCCGCAACCTGACCAACCGCATTCGCTTCATGCAGGGCTTTACGAATGa > 1:2131016/1‑71 (MQ=255)
aTCACATATCGCCGGAACACCGCCCGCAACCTGACCAACCGAATTCGCTTCATGCAGGGCTTTACGAATGa > 1:2154434/1‑71 (MQ=255)
tCACATATCGCCGGAACACCGCCCGCAACCTGACCAACCGCATTCGCTTCATGCAGGGCTTTACGAAt < 1:2387398/68‑1 (MQ=255)
tCACATATCGCCGGAACACCGCCCGCAACCTGACCAACCGCATTCGCTTCATGCAGGGCTTTACGAATGAt < 1:2247492/71‑1 (MQ=255)
cacataTCGCCGGAACACCGCCCGCAACCTGACCAACCGCATTCGCTTCa > 1:70587/1‑50 (MQ=255)
cacataTCGCCGGAACACCGCCCGCAACCTGACCAACCGCATTCGCTTCATGCAGGGCTTTACGAATGAtt > 1:3471871/1‑71 (MQ=255)
acataTCGCCGGAACACCGCCCGCAACCTGACCAACCGCa > 1:2329057/1‑40 (MQ=255)
acataTCGCCGGAACACCGCCCGCAACCTGACCAACCGCATTCGCTTCATGCAGGGCTTTACGAATGAttt > 1:1627427/1‑71 (MQ=255)
acataTCGCCGGAACACCGCCCGCAACCTGACCAACCGCATTCGCTTCATGCAGGGCTTTACGAATGAtt > 1:4385612/1‑70 (MQ=255)
acataTCGCCGGAACACCGCCCGCAACCTGACCAACCGCATTCGCTTCATGCAGGGCTTTACGAATGAtt > 1:3980981/1‑70 (MQ=255)
|
ATCGCTATCACTTCGCGGCTTAGCAGCGACAATTCCATTCCATCCTGCCCCTGGGTGACACCATCACACATCGCCGGAACACCGCCCGCAACCTGACCAACCGCATTCGCTTCATGCAGGGCTTTACGAATGATTT > W3110S.gb/1935918‑1936053
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
