Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F28 I1 R2
|
328 |
63.0 |
4687567 |
98.0% |
4593815 |
65.2 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
1,962,205 |
C→T |
R144C (CGT→TGT) |
argS → |
arginyl‑tRNA synthetase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 1,962,205 | 0 | C | T | 100.0%
| 99.6
/ NA
| 34 | R144C (CGT→TGT) | argS | arginyl‑tRNA synthetase |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (15/19); total (15/19) |
GCCAAACGTGGCGAAAGAGATGCATGTCGGTCACCTGCGCTCTACCATTATTGGTGACGCAGCAGTGCGTACTCTGGAGTTCCTCGGTCACAAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTT > W3110S.gb/1962138‑1962272
|
gcCAAACGTGGCGAAAGAGATGCATGTCGGTCACCTGCGCTCTACCATTATTGGTGACGCAGCAGTGTGTa < 1:4535909/71‑1 (MQ=255)
gcCAAACGTGGCGAAAGAGATGCATGTCGGTCACCTGCGCTCTACCATTATTGGTGACGCAGCAGTGTGTa < 1:3952537/71‑1 (MQ=255)
aaCGTGGCGAAAGAGATGCATGTCGGTCACCTGCGCTCTACCATTATTGGTGACGCAGCAGTGTGTActct > 1:3009733/1‑71 (MQ=255)
aCGTGGCGAAAGAGATGCATGTCGGTCACCTGCGCTCTACCATTATTGGTGACGCAGCAGTGTGTACTCTg < 1:910676/71‑1 (MQ=255)
agagATGCATGTCGGTCACCTGCGCTCTACCATTATTGGTGACGCAGCAGTGTGTACTCTGGAGTTCCTCg > 1:2505114/1‑71 (MQ=255)
gCATGTCGGTCACCTGCGCTCTACCATTATTGGTGACGCAGCAGTGTGTACTCTGTAGTTCCTCGGTcac > 1:1141555/1‑70 (MQ=255)
cATGTCGGTCACCTGCGCTCTACCATTATTGGTGACGCAGCAGTGTGTACTCTGGAGTTCCTCGGTCACaa < 1:3660115/71‑1 (MQ=255)
tCGGTCACCTGCGCTCTACCATTATTGGTGACGCAGCAGTGTGTACTCTGGAGTTCCTCGGTCACAAAGt > 1:1761690/1‑70 (MQ=255)
tCGGTCACCTGCGCTCTACCATTATTGGTGACGCAGCAGTGTGTACTCTGGAGTTCCTCGGTCACAAAGt < 1:2286602/70‑1 (MQ=255)
ggTCACCTGCGCTCTACCATTATTGGTGACGCAGCAGTGTGTACTCTGGAGTTCCTCGGTCACAAAGTGAt > 1:1827276/1‑71 (MQ=255)
cACCTGCGCTCTACCATTATTGGTGACGCAGCAGTGTGTACTCTGGAGTTCCTCGGTCACAAAGTGATTcg > 1:2416020/1‑71 (MQ=255)
tGCGCTCTACCATTATTGGTGACGCAGCAGTGTGTACTCTgg < 1:1254716/42‑1 (MQ=255)
tGCGCTCTACCATTATTGGTGACGCAGCAGTGTGTACTCTGGAGTTCCTCGGTCACAAAg > 1:1733002/1‑60 (MQ=255)
tGCGCTCTACCATTATTGGTGACGCAGCAGTGTGTACTCTGGAGTTCCTCGGTCACAAAGTGATTCgcgc > 1:4030588/1‑70 (MQ=255)
gcgcTCTACCATTATTGGTGACGCAGCAGTGTGTACTCTGGAGTTCCTCGGTCACAAAGTGATTCGCGCa > 1:832638/1‑70 (MQ=255)
gcTCTACCATTATTGGTGACGCAGCAGTGTGTACTCTGGAGTTCCTCGGTCACAAAGTGATTCGCGCAAAc < 1:2831574/71‑1 (MQ=255)
tACCATTATTGGTGACGCAGCAGTGTGTACTCTGGAGTTCCTCGGTCACaa < 1:3614095/51‑1 (MQ=255)
tACCATTATTGGTGACGCAGCAGTGTGTACTCTGGAGTTCCTCGGTCACaa < 1:934925/51‑1 (MQ=255)
tACCATTATTGGTGACGCAGCAGTGTGTACTCTGGAGTTCCTCGGTCACAAAGTGATTCGCGCAAACCa < 1:1661181/69‑1 (MQ=255)
ccATTATTGGTGACGCAGCAGTGTGTACTCTGGAGTTCCTCGGTCACAAAGTGATTCGCGCAAACCACGTc > 1:326476/1‑71 (MQ=255)
ccATTATTGGTGACGCAGCAGTGTGTACTCTGGAGTTCCTCGGTCACAAAGTGATTCGCGCAAACCACGTc > 1:2334135/1‑71 (MQ=255)
ccATTATTGGTGACGCAGCAGTGTGTACTCTGGAGTTCCTCGGTCACAAAGTGATTCGCGCAAACCACGTc > 1:222833/1‑71 (MQ=255)
ccATTATTGGTGACGCAGCAGTGTGTACTCTGGAGTTCCTCGGTCACAAAGTGATTCGCGCAAACCACGTc > 1:3983333/1‑71 (MQ=255)
tattGGTGACGCAGCAGTGTGTACTCTGGAGTTCCTCGGTCACAAAGTGATTCGCGCAAACCACGTCGGc < 1:4002146/70‑1 (MQ=255)
tattGGTGACGCAGCAGTGTGTACTCTGGAGTTCCTCGGTCACAAAGTGATTCGCGCAAACCACGTCGGc < 1:4447954/70‑1 (MQ=255)
tattGGTGACGCAGCAGTGTGTACTCTGGAGTTCCTCGGTCACAAAGTGATTCGCGCAAACCACGTCGGc < 1:418571/70‑1 (MQ=255)
attGGTGACGCAGCAGTGTGTACTCTGGAGTTCCTCGGTCACAAAGTGATTCGCGCAAACCACGTCGGCg < 1:2122646/70‑1 (MQ=255)
attGGTGACGCAGCAGTGTGTACTCTGGAGTTCCTCGGTCACAAAGTGATTCGCGCAAACCACGTCGGCg < 1:463659/70‑1 (MQ=255)
attGGTGACGCAGCAGTGTGTACTCTGGAGTTCCTAGGTCACAAAGTGATTCGCGCAAACCACGTCGGCGa > 1:851414/1‑71 (MQ=255)
ttGGTGACGCAGCAGTGTGTACTCTGGAGTTCCTCGGTCACAAAGTGATTCGCGCAAACCACGTCGGCg < 1:1513424/69‑1 (MQ=255)
ttGGTGACGCAGCAGTGTGTACTCTGGAGTTCCTCGGTCACAAAGTGATTCGCGCAAACAACGTCGGCg < 1:2563496/69‑1 (MQ=255)
ggTGACGCAGCAGTGTGTACTCTGGAGTTCCTCGGTCACAAAGTGATTCGCGCAAACCACGTCGGCGACTg > 1:1763714/1‑71 (MQ=255)
aCGCAGCAGTGTGTACTCTGGAGTTCCTCGGTCACAAAGTTATTCGCGCAAACCACGTCGGCGACTGGGGc < 1:3493644/71‑1 (MQ=255)
gcagTGTGTACTCTGGAGTTCCTCGGTCACAAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCa < 1:2317695/71‑1 (MQ=255)
gtgtgtACTCTGGAGTTCCTCGGTCACAAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGtt > 1:1144268/1‑71 (MQ=255)
|
GCCAAACGTGGCGAAAGAGATGCATGTCGGTCACCTGCGCTCTACCATTATTGGTGACGCAGCAGTGCGTACTCTGGAGTTCCTCGGTCACAAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTT > W3110S.gb/1962138‑1962272
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
