Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F28 I1 R2
|
328 |
63.0 |
4687567 |
98.0% |
4593815 |
65.2 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
2,264,229 |
C→T |
L172L (CTG→CTA) |
fruA ← |
fused fructose‑specific PTS enzyme IIB'BC components |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 2,264,229 | 0 | C | T | 100.0%
| 196.2
/ NA
| 66 | L172L (CTG→CTA) | fruA | fused fructose‑specific PTS enzyme IIB'BC components |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (28/38); total (28/38) |
CCTGCGCGGTTTTCTTCAGCGCCAGACCGGTAGAGGTACGATACATCGGTTTACCAGCAAATTTCGCCAGATCCACTTCGATATCTGCCGCCACAATCACCAGATCCGCTGCTGCGACTTCTTCGGGAGTGAT > W3110S.gb/2264162‑2264294
|
ccTGCGCGGTTTTCTTCAGCGCCAGACCGGTAGAGGTACGATACATCGGTTTACCAGCAAATTTCGCTAg < 1:182688/70‑1 (MQ=255)
cgcgGTTTTCTTCAGCGCCAGACCGGTAGAGGTACGATACATCGGTTTACCAGCAAATTTCGCTAGATc < 1:2315985/69‑1 (MQ=255)
cgcgGTTTTCTTCAGCGCCAGACCGGTAGAGGTACGATACATCGGTTTACCAGCAAATTTCGCTAGATc < 1:3611109/69‑1 (MQ=255)
cgcgGTTTTCTTCAGCGCCAGACCGGTAGAGGTACGATACATCGGTTTACCAGCAAATTTCGCTAGATc < 1:879763/69‑1 (MQ=255)
cgcgGTTTTCTTCAGCGCCAGACCGGTAGAGGTACGATACATCGGTTTACCAGCAAATTTCGCTAGATc < 1:658643/69‑1 (MQ=255)
gTTTTCTTCAGCGCCAGACCGGTAGAGGTACGATACATCGGTTTACCAGCAAATTTCGCTAGATCCACTTc < 1:3776771/71‑1 (MQ=255)
gTTTTCTTCAGCGCCAGACCGGTAGAGGTACGATACATCGGTTTACCAGCAAATTTCGCTAGATCCACTTc < 1:4083766/71‑1 (MQ=255)
ttttCTTCAGCGCCAGACCGGTAGAGGTACGATACATCGGTTTACCAGCAAATTTCGCTAGATCCACTTc > 1:3827428/1‑70 (MQ=255)
ttcttcAGCGCCAGACCGGTAGATGTACGATACATCGGTTTACCAGCAAATTTCGCTAGATCCACTTCGa < 1:1493091/70‑1 (MQ=255)
ttcttcAGCGCCAGACCGGTAGAGGTACGATACATCGGTTTACCAGCAAATTTCGCTAGATCCACTTCGa > 1:1416016/1‑70 (MQ=255)
ttcttcAGCGCCAGACCGGTAGAGGTACGATACATCGGTTTACCAGCAAATTTCGCTAGATCCACTTCGa < 1:1494280/70‑1 (MQ=255)
ctcttcAGCGCCAGACCGTTAGAGGTACGATACATCGGTTTACCAGCAAATTTCGCTAGATCCACTTCGa < 1:2535387/69‑1 (MQ=255)
ctcttcAGCGCCAGACCGGTAGAGGTACGATACATCGGTTTACCAGCAAATTTCGCTAGATCCACTTCGa < 1:705636/69‑1 (MQ=255)
ctcttcAGCGCCAGACCGGTAGAGGTACGATACATCGGTTTACCAGCAAATTTCGCTAGATCCACTTCGa < 1:3468555/69‑1 (MQ=255)
tcttcAGCGCCAGACCGGTAGAGGTACGATACATCGGTTTACCAGCAAATTTCGCTAGATCCACTTCGat < 1:3871544/70‑1 (MQ=255)
tcttcAGCGCCAGACCGGTAGAGGTACGATACATCGGTTTACCAGCAAATTTCGCTAGATCCACTTCGat < 1:1285638/70‑1 (MQ=255)
gACCGGTAGAGGTACGATACATCGGTTTACCAGCAAATTTCGCTAg < 1:791508/46‑1 (MQ=255)
ccGGTAGAGGTACGATACATCGGTTTACCAGCAAATTTCGCTAGATCCACTTCGATATc > 1:4071265/1‑59 (MQ=255)
ccGGTAGAGGTACGATACATCGGTTTACCAGCAAATTTCGCTAGATCCACTTCGATATCTGCCGCCACAAt < 1:88084/71‑1 (MQ=255)
ccGGTAGAGGTACGATACATCGGTTTACCAGCAAATTTCGCTAGATCCACTTCGATATCTGCCGCCACAAt < 1:3855062/71‑1 (MQ=255)
agGTACGATACATCGGTTTACCAGCAAATTTCGCTAGATCCACTTc < 1:3544447/46‑1 (MQ=255)
agGTACGATACATCGGTTTACCAGCAAATTTCGCTAGATCCACTTCGATATCTGCCGCCACAATCACCAGa < 1:371479/71‑1 (MQ=255)
ggTACGATACATCGGTTTACCAGCAAATTTCGCTAGATCCACTTCGATATCTGCCGCCACAATCAc > 1:1760950/1‑66 (MQ=255)
ggTACGATACATCGGTTTACCAGCAAATTTCGCTAGATCCACTTCGATATCTGCCGCCACAATCAc > 1:3312405/1‑66 (MQ=255)
gTACGATACATCGGTTTACCAGCAAATTTCGCTAGATCCACTTCGa > 1:899104/1‑46 (MQ=255)
gTACGATACATCGGTTTACCAGCAAATTTCGCTAGATCCACTTCGATATCTGCCGCCACAATCACCAGATc > 1:2413145/1‑71 (MQ=255)
tACGATACATCGGTTTACCAGCAAATTTCGCTAGATCCACTTAGata < 1:2999242/47‑1 (MQ=255)
aCGATACATCGGTTTACCAGCAAATTTCGCTAGATCCACTTCGATATCTGCCGCCACAAt > 1:3742523/1‑60 (MQ=255)
aCGATACATCGGTTTACCAGCAAATTTCGCTAGATCCACTTCGATATCTGCCGCCACAATCACCAGATCCg > 1:4581754/1‑71 (MQ=255)
aCGATACATCGGTTTACCAGCAAATTTCGCTAGATCCACTTCGATATCTGCCGCCACAATCACCAGATCCg > 1:4587049/1‑71 (MQ=255)
aCGATACATCGGTTTACCAGCAAATTTCGCTAGATCCACTTCGATATCTGCCGCCACAATCACCAGATCCg > 1:1280626/1‑71 (MQ=255)
cGATACATCGGTTTACCAGCAAATTTCGCTAGATCCACTTCGat < 1:954435/44‑1 (MQ=255)
cGATACATCGGTTTACCAGCAAATTTCGCTAGATCCACTTCGat < 1:318943/44‑1 (MQ=255)
cGATACATCGGTTTACCAGCAAATTTCGCTAGATCCACTTCGa > 1:2319518/1‑43 (MQ=255)
cGATACATCGGTTTACCAGCAAATTTCGCTAGATCCACTTCGATATc > 1:2814350/1‑47 (MQ=255)
cGATACATCGGTTTACCAGCAAATTTCGCTAGATCCACTTCGATATc > 1:1626559/1‑47 (MQ=255)
cGATACATCGGTTTACCAGCAAATTTCGCTAGATCCACTTCGATATCTGCCGCCACAATCACCAGATCCgc < 1:2834426/71‑1 (MQ=255)
cGATACATCGGTTTACCAGCAAATTTCGCTAGATCCACTTCGATATCTGCCGCCACAATCACCAGATCCgc > 1:143830/1‑71 (MQ=255)
cGATACATCGGTTTACCAGCAAATTTCGCTAGATCCACTTCGATATCTGCCGCCACAATCACCAGATCCgc > 1:1570828/1‑71 (MQ=255)
cGATACATCGGTTTACCAGCAAATTTCGCTAGATCCACTTCGATATCTGCCGCCACAATCACCAGATCCgc > 1:1905570/1‑71 (MQ=255)
cGATACATCGGTTTACCAGCAAATTTCGCTAGATCCACTTCGATATCTGCCGCCACAATCACCAGATCCg > 1:1347667/1‑70 (MQ=255)
gATACATCGGTTTACCAGCAAATTTCGCTAGATCCACTTCGATATCTGCCGCCACAATCACCAGATCCgct < 1:1294390/71‑1 (MQ=255)
aCATCGGTTTACCAGCAAATTTCGCTAGATCCACTTCGa > 1:286020/1‑39 (MQ=255)
aCATCGGTTTACCAGCAAATTTCGCTAGATCCACTTCGATATCTGCCGCCACAATCACCAGATCCgctgct > 1:4541317/1‑71 (MQ=255)
aCATCGGTTTACCAGCAAATTTCGCTAGATCCACTTCGATATCTGCCGCCACAATCACCAGATCCgctgct > 1:1670705/1‑71 (MQ=255)
cATCGGTTTACCAGCAAATTTCGCTAGATCCACTTCGATATCTGCCGCCACAATCACCAGATCCGctgctg > 1:385475/1‑71 (MQ=255)
aTCGGTTTACCAGCAAATTTCGCTAGATCCACTTCGATATCTGCCGCCACAATCTCCAGATCCGCtgctgc < 1:101052/71‑1 (MQ=255)
aTCGGTTTACCAGCAAATTTCGCTAGATCCACTTCGATATCTGCCGCCACAATCACCAGATCCGCtgctgc < 1:4611239/71‑1 (MQ=255)
tCGGTTTACCAGCAAATTTCGCTAGATCCACTTCGATATc > 1:2711447/1‑40 (MQ=255)
tCGGTTTACCAGCAAATTTCGCTAGATCCACTTCGATATCTGCCGCCACAATCACCAGATCCGCTGCTGCg < 1:1674317/71‑1 (MQ=255)
tCGGTTTACCAGCAAATTTCGCTAGATCCACTTCGATATCTGCCGCCACAATCACCAGATCCGCTGCTGCg < 1:2269955/71‑1 (MQ=255)
ggTTTACCAGCAAATTTCGCTAGATCCACTTCGATATCTGCCGCCACAATCACCAGATCCGCTGCTGCGAc > 1:1642635/1‑71 (MQ=255)
tttACGAGCAAATTTCGCTAGATCCACTTGGATATCTGCCGCCACAATCACCAGATCCGCTGCTGCGActt < 1:626206/71‑1 (MQ=255)
tACCAGCAAATTTCGCTAGATCCACTTCGATATCTGCCGCCACaa > 1:2059541/1‑45 (MQ=255)
tACCAGCAAATTTCGCTAGATCCACTTCGATATCTGCCGCCACaa > 1:2054234/1‑45 (MQ=255)
tACCAGCAAATTTCGCTAGATCCACTTCGATATCTGCCGCCACaa > 1:1323257/1‑45 (MQ=255)
gCAAATTTCGCTGGATCCACTTCGATATCTGCCGCCACAATCACCAGATCCGCTGCTGCGACTTCTTCggg < 1:4663681/71‑1 (MQ=255)
gCAAATTTCGCTAGATCCACTTCGATATCTGCCGCCACAATCACCAGATCCGCTGCTGCGACTTCTTCggg < 1:1291803/71‑1 (MQ=255)
cAAATTTCGCTAGATCCACTTCGATATCTGCCGCCACAATCACCAGATCCGCTGCTGCGACTTCTTCggg < 1:4492952/70‑1 (MQ=255)
aaTTTCGCTAGATCCACTTCGATATCTGCCGCCACAATCACCAGATCCGCTGCTGCGACTTCTTCGGGAGt < 1:2460174/71‑1 (MQ=255)
aaTTTCGCTAGATCCACTTCGATATCTGCCGCCACAATCACCAGATCCGCTGCTGCGACTTCTTCGGGAGt < 1:4490944/71‑1 (MQ=255)
aaTTTCGCTAGATCCACTTCGATATCTGCCGCCACAATCACCAGATCCGCTGCTGCGACTTCTTCGGGAGt < 1:3237751/71‑1 (MQ=255)
aTTTCGCTAGATCCACTTCGATATCTGCCGCCACAATCACCAGATCCGCTGCTGCGACTTCTTCGGGAGTg < 1:689042/71‑1 (MQ=255)
aTTTCGCTAGATCCACTTCGATATCTGCCGCCACAATCACCAGATCCGCTGCTGCGACTTCTTCGGGAGTg < 1:1014266/71‑1 (MQ=255)
aTTTCGCTAGATCCACTTCGATATCTGCCGCCACAATCACCAGATCCGCTGCTGCGACTTCTTCGGGAGTg < 1:2889321/71‑1 (MQ=255)
ttCGCTAGATCCACTTCGATATCTGCCGCCACAATCACCAGATCCGCTGCTGCGACTTCTTCGGGAGTGAt > 1:667138/1‑71 (MQ=255)
|
CCTGCGCGGTTTTCTTCAGCGCCAGACCGGTAGAGGTACGATACATCGGTTTACCAGCAAATTTCGCCAGATCCACTTCGATATCTGCCGCCACAATCACCAGATCCGCTGCTGCGACTTCTTCGGGAGTGAT > W3110S.gb/2264162‑2264294
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
