Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F28 I1 R2
|
328 |
63.0 |
4687567 |
98.0% |
4593815 |
65.2 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
2,512,230 |
A→G |
W371R (TGG→CGG) |
ypdG ← |
predicted enzyme IIC component of PTS |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 2,512,230 | 0 | A | G | 100.0%
| 120.4
/ NA
| 36 | W371R (TGG→CGG) | ypdG | predicted enzyme IIC component of PTS |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (23/13); total (23/13) |
TTATGACTGCCCCCAGCGCGATCCCCGCCATATAGACGCCCAGGTTAGTGACCAGCGGCCATGCCCAGATAGCGGACTCCGGGAACCATTGCACTGCGCCCAGCCAGACAGCGGCGGTTGAGCCGACAATAGCG > W3110S.gb/2512164‑2512297
|
ttATGACTGCCCCCAGCGCGATCCCCGCCATATAGACGCCCAGGTTAGTGACCAGCGGCCATGCCCGGa < 1:1960568/69‑1 (MQ=255)
aTGACTGCCCCCAGCGCGATCCTCGCCATATAGACGCCCAGGTTAGTGACCAGCGGCCATGCCCGGATAGc > 1:1234454/1‑71 (MQ=255)
aTGACTGCCCCCAGCGCGATCCCCGCCATATAGACGCCCAGGTTAGTGACCAGCGGCCATGCCCGGATAg > 1:521954/1‑70 (MQ=255)
tGACTGCCCCCAGCGCGATCCCCGCCATATAGACGCCCAGGTTAGTGACCAGCGGCCATGCCCGGATAGCg < 1:550750/71‑1 (MQ=255)
cTGCCCCCAGCGCGATCCCCGCCATATAGACGCCCAGGTTAGTGACCAGCGTCCATGCCCGGATAGCGGAc < 1:954324/71‑1 (MQ=255)
gCCCCCAGCGCGATCCCCGCCATATAGACGCCCAGGTTAGTGACTAGCGGCCATGCCCGGATAGCGGACTc > 1:214424/1‑71 (MQ=255)
cgcgATCCCCGCCATATAGACGCCCAGGTTAGTGACCAGCGGCCATGCCCGGATAGCGGACTCCGGGAAcc < 1:1486626/71‑1 (MQ=255)
gcgATCCCCGCCATATAGACGCCCAGGTTAGTGACCAGCGGCCATGCCCGGATAGCGGACTCCGGGAACCa > 1:2271472/1‑71 (MQ=255)
aTCCCCGCCATATAGACGCCCAGGTTAGTGACCAGCGGCCATGCCCGGATAGCGGACTCCGGGAACCATTg < 1:3452973/71‑1 (MQ=255)
tCCCCGCCATATAGACGCCCAGGTTAGTGACCAGCGGCCATGCCCGGATAGCGGACTCCGGGAACCATTg < 1:4447456/70‑1 (MQ=255)
ataGACGCCCAGGTTAGTGACCAGCGGCCATGCCCGGATAGCGGACTCCGGGAACCATTGCACTGCGcc > 1:4492414/1‑69 (MQ=255)
taGACGCCCAGGTTAGTGACCAGCGGCCATGCCCGGATAGCGGACTCCGGGAACCATTGCACTGCGCccag > 1:1888438/1‑71 (MQ=255)
taGACGCCCAGGTTAGTGACCAGCGGCCATGCCCGGATAGCGGACTCCGGGAACCATTGCACTGCGCccag > 1:2766707/1‑71 (MQ=255)
taGACGCCCAGGTTAGTGACCAGCGGCCATGCCCGGATAGCGGACTCCGGGAACCATTGCACTGCGCccag > 1:1765375/1‑71 (MQ=255)
taGACGCCCAGGTTAGTGACCAGCGGCCATGCCCGGATAGCGGACTCCGGGAACCATTGCACTGCGCccag > 1:530116/1‑71 (MQ=255)
taGACGCCCAGGTTAGTGACCAGCGGCCATGCCCGGATAGCGGACTCCGGGAACCATTGCACTGCGCccag > 1:1523125/1‑71 (MQ=255)
taGACGCCCAGGTTAGTGACCAGCGGCCATGCCCGGATAGCGGACTCCGGGAACCATTGCACTGCGCccag > 1:4299022/1‑71 (MQ=255)
taGACGCCCAGGTTAGTGACCAGCGGCCATGCCCGGATAGCGGACTCCGGGAACCATTGCACTGCGCccag > 1:4270261/1‑71 (MQ=255)
taGACGCCCAGGTTAGTGACCAGCGGCCATGCCCGGATAGCGGACTCCGGGAACCATTGCACTGCGCccag > 1:4217587/1‑71 (MQ=255)
aCGCCCAGGTTAGTGACCAGCGGCCATGCCCGGATAGCGGACTCCGGGAACCATTGCACTGCGCccagcca < 1:4182725/71‑1 (MQ=255)
tAGTGACCAGCGGCCATGCCCGGATAGCGGACTCCGGGAACCATTGCACTGCGCCCAGCCAGACAgcggcg > 1:16880/1‑71 (MQ=255)
aGTGACCAGCGGCCATGCCCGGATAGCGGACTCCGGGAACCATTGCACTGCGCCCAGCCAGACAGcggcgg > 1:457148/1‑71 (MQ=255)
gTGACCAGCGGCCATGCCCGGATAGCGGACTCCGGGAACCATTGCACTGCGCCCAGCCAGACAGcggcgg < 1:3162928/70‑1 (MQ=255)
gTGACCAGCGGCCATGCCCGGATAGCGGACTCCGGGAACCATTGCACTGCGCCCAGCCAGACAGcggcgg < 1:3996682/70‑1 (MQ=255)
ggCCATGCCCGGATAGCGGACTCCGGGAACCATTGCACTGCGCCCAGCCAGACAGCGGCGGTTGAGCCGAc < 1:976808/71‑1 (MQ=255)
gCCATGCCCGGATAGCGGACTCCGGGAACCATTGCACTGCGCCCAGCCAGACAGCGGCGGTTGAGCCGAc < 1:406681/70‑1 (MQ=255)
tGCCCGGATAGCGGACTCCGGGAACCATTGCACTGCGCCCAGCCAGACAGCGGCGGTTGAGCCGAc < 1:3917318/66‑1 (MQ=255)
tGCCCGGATAGCGGACTCCGGGAACCATTGCACTGCGCCCAGCCAGACAGCGGCGGTTGAGCCGAc < 1:371586/66‑1 (MQ=255)
gCCCGGATAGCGGACTCCGGGAACCATTGCACTGCGCCCAGCCAGACAGCGGCGGTTGAGCCGACAATAgc > 1:4397631/1‑71 (MQ=255)
cccGGATAGCGGACTCCGGGAACCATTGCACTGCGCCCAGCCGGACAGCGGCGGTTGAGCCGACAATAgc > 1:1338771/1‑70 (MQ=255)
cccGGATAGCGGACTCCGGGAACCATTGCACTGCGCCCAGCCAGAc > 1:3789353/1‑46 (MQ=255)
cccGGATAGCGGACTCCGGGAACCATTGCACTGCGCCCAGCCAGACAGCGGCGGTTGAGCCGACaa > 1:3944892/1‑66 (MQ=255)
cccGGATAGCGGACTCCGGGAACCATTGCACTGCGCCCAGCCAGACAGCGGCGGTTGAGCCGACaa > 1:3646258/1‑66 (MQ=255)
cccGGATAGCGGACTCCGGGAACCATTGCACTGCGCCCAGCCAGACAGCGGCGGTTGAGCCGACAATAgcg > 1:3286219/1‑71 (MQ=255)
cccGGATAGCGGACTCCGGGAACCATTGCACTGCGCCCAGCCAGACAGCGGCGGTTGAGCCGACAATAgcg > 1:3021993/1‑71 (MQ=255)
cccGGATAGCGGACTCCGGGAACCATTGCACTGCGCCCAGCCAGACAGCGGCGGTTGAGCCGACAATAgcg > 1:1767310/1‑71 (MQ=255)
|
TTATGACTGCCCCCAGCGCGATCCCCGCCATATAGACGCCCAGGTTAGTGACCAGCGGCCATGCCCAGATAGCGGACTCCGGGAACCATTGCACTGCGCCCAGCCAGACAGCGGCGGTTGAGCCGACAATAGCG > W3110S.gb/2512164‑2512297
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
