Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F28 I1 R2
|
328 |
63.0 |
4687567 |
98.0% |
4593815 |
65.2 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
2,855,194 |
A→T |
I90N (ATC→AAC) |
ygbA ← |
hypothetical protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 2,855,194 | 0 | A | T | 100.0%
| 122.2
/ NA
| 41 | I90N (ATC→AAC) | ygbA | hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base T (22/19); total (22/19) |
GGACGATATTTTTCCGGCAGTTCTGGCACCGGACGCTTGTCATCGATGAGATGACGCACGGTTAAGATCGGATGACGCCACAGCATTCTCGGCCCGGCCCAACGCATAATCTGTTTCATCTCTTCACGCTTTG > W3110S.gb/2855128‑2855260
|
ggACGATATTTTTCCGGCAGTTCTGGCACCGGACGCTTGTCATCGATGAGATGACGCACGGTTAAGTTCg < 1:1233530/70‑1 (MQ=255)
ggACGATATTTTTCCGGCAGTTCTGGCACCGGACGCTTGTCATCGATGAGATGACGCACGGTTAAGTTCg < 1:3652961/70‑1 (MQ=255)
tttttCCGGCAGTTCTGGCACCGGACGCTTGTCATCGATGAGATGACGCACGGTTAAGTTCGGATGACGc > 1:2833512/1‑70 (MQ=255)
tttttCCGGCAGTTCTGGCACCGGACGCTTGTCATCGATGAGATGACGCACGGTTAAGTTCGGATGACGc > 1:4548128/1‑70 (MQ=255)
ccGGCAGTTCTGGCACCGGACGCTTGTCATCGATGAGATGACGCACGGTTAAGTTCGGATGACGCCACAg < 1:2737053/70‑1 (MQ=255)
ccGGCAGTTCTGGCACCGGACGCTTGTCATCGATGAGATGACGCACGGTTAAGTTCGGATGACGCCACAg < 1:2498559/70‑1 (MQ=255)
cGGCAGTTCTGGCACCGGACGCTTGTCATCGATGAGATGACGCACGGTTAAGTTCGGATGACGCCACAGc < 1:2648757/70‑1 (MQ=255)
ggCAGTTCTGGCACCGGACGCTTGTCATCGATGAGATGACGCACGGTTAAGTTCGGATGACGCCACAGCAt < 1:4048093/71‑1 (MQ=255)
gTTCTGGCACCGGACGCTTGTCATCGATGAGATGACGCACGGTTAAGTTCg < 1:3216675/51‑1 (MQ=255)
tCTGGCACCGGACGCTTGTCATCGATGAGATGACGCACGGTTAAGTTCGGATGACGCCACAGCATTCTcgg > 1:2777502/1‑71 (MQ=255)
cTGGCACCGGACGCTTGTCATCGATGAGATGACGCACGGTTAAGTTCGGATGACGCCACAGCATTCTcggc > 1:1635341/1‑71 (MQ=255)
cTGGCACCGGACGCTTGTCATCGATGAGATGACGCACGGTTAAGTTCGGATGACGCCACAGCATTCTcggc > 1:4645150/1‑71 (MQ=255)
cTGGCACCGGACGCTTGTCATCGATGAGATGACGCACGGTTAAGTTCGGATGACGCCACAGCATTCTcggc > 1:2876709/1‑71 (MQ=255)
cTGGCACCGGACGCTTGTCATCGATGAGATGACGCACGGTTAAGTTCGGATGACGCCACAGCATTCTcggc > 1:3626463/1‑71 (MQ=255)
cACCGGACGCTTGTCATCGATGAGATGACGCACGGTTAAGTTCGGATGACGCCACAg < 1:3938261/57‑1 (MQ=255)
cGGACGCTTGTCATCGATGAGATGACGCACGGTTAAGTTCGGATGACGCCACAGCATTCTcggcccggcc > 1:3543739/1‑70 (MQ=255)
cGGACGCTTGTCATCGATGAGATGACGCACGGTTAAGTTCGGATGACGCCACAGCATTCTCggcccggccc < 1:4005312/71‑1 (MQ=255)
gACGCTTGTCATCGATGAGATGACGCACGGTTAAGTTCGGATGACGCCACAGCATTCTCGGCCCGGCCCaa > 1:917346/1‑71 (MQ=255)
gACGCTTGTCATCGATGAGATGACGCACGGTTAAGTTCGGATGACGCCACAGCATTCTCGGCCCGGCCCaa > 1:1399636/1‑71 (MQ=255)
cTTGTCATCGATGAGATGACGCACGGTTAAGTTCGGATGACGCCACAg < 1:3314602/48‑1 (MQ=255)
gTCATCGATGAGATGACGCACGGTTAAGTTCGGATGACGCCACAGCATTCTCGGCCCGGCCCAACGCAt < 1:611361/69‑1 (MQ=255)
tCATCGATGAGATGACGCACGGTTAAGTTCGGATGACGCCACAGCATTCTCGGCCCGGCCCAACGCATaa < 1:584221/70‑1 (MQ=255)
tCGATGAGATGACGCACGGTTAAGTTCGGATGACGCCACAg < 1:695549/41‑1 (MQ=255)
gatgagatgaCGCACGGTTAAGTTCGGATGACGCCACAGCATTCTCGGCCCGGCCCAACGCATAATCTGtt > 1:646919/1‑71 (MQ=255)
gatgagatgaCGCACGGTTAAGTTCGGATGACGCCACAGCATTCTCGGCCCGGCCCAACGCATAATCTGtt > 1:101475/1‑71 (MQ=255)
gatgagatgaCGCACGGTTAAGTTCGGATGACGCCACAGCATTCTCGGCCCGGCCCAACGCATAATCTGtt > 1:1380052/1‑71 (MQ=255)
gatgagatgaCGCACGGTTAAGTTCGGATGACGCCACAGCATTCTCGGCCCGGCCCAACGCATAATCTGt < 1:4683993/70‑1 (MQ=255)
tgagatgaCGCACGGTTAAGTTCGGATGACGCCACAGCATTCTCGGCCCGGCCCAACGCATAATCTGttt > 1:3038891/1‑70 (MQ=255)
gagatgaCGCACGGTTAAGTTCGGATGACGCCACAGCATTCTCGGCCCGGCCCAACGCATAATCTGTTTCa > 1:3286573/1‑71 (MQ=255)
gaCGCACGGTTAAGTTCGGATGACGCCACAGCATTCTCGGCCCGGCCCAACGCATAATCTGTTTCATctct > 1:3184173/1‑71 (MQ=255)
gaCGCACGGTTAAGTTCGGATGACGCCACAGCATTCTCGGCCCGGCCCAACGCATAATCTGTTTCATctct > 1:4351534/1‑71 (MQ=255)
gaCGCACGGTTAAGTTCGGATGACGCCACAGCATTCTCGGCCCGGCCCAACGCATAATCTGTTTCATctct > 1:1183965/1‑71 (MQ=255)
gaCGCACGGTTAAGTTCGGATGACGCCACAGCATTCTCGGCCCGGCCCAACGCATAATCTGTTTCATctct > 1:1000227/1‑71 (MQ=255)
aCGCACGGTTAAGTTCGGATGACGCCACAGCATTCTCGGCCCGGCCCAACGCATAATCTGTTTCATCTCtt < 1:2434697/71‑1 (MQ=255)
aCGCACGGTTAAGTTCGGATGACGCCACAGCATTCTCGGCCCGGCCCAACGCATAATCTGTTTCATCTCtt < 1:4015166/71‑1 (MQ=255)
aCGCACGGTTAAGTTCGGATGACGCCACAGCATTCTCGGCCCGGCCCAACGCATAATCTGTTTCATCTCtt < 1:1545498/71‑1 (MQ=255)
cGCACGGTTAAGTTCGGATGACGCCACAGCATTCTCGGCCCGGCCCAACGCATAATCTTTTTCATCTCtt > 1:1732914/1‑70 (MQ=255)
aCGGTTAAGTTCGGATGACGCCACAGCATTCTCGGCCCGGCCCAACGCATAATCTGTTTCATCTCTTCACg < 1:1198283/71‑1 (MQ=255)
gggTTAAGTTCGGATGACGCCACAGCATTCTCGGCCCGGCCCAACGCATAATCTGTTTCATCTCTTCACGc > 1:2443900/2‑71 (MQ=255)
ttAAGTTCGGATGACGCCACAGCATTCTCGGCCCGGCCCAACGCATAATCTGTTTCATCTCTTCACGCttt > 1:1577393/1‑71 (MQ=255)
aaGTTCGGATGACGCCACAGCATTCTCGGCCCGGCCCAACGCATAATCTGTTTCATCTCTTCACGCTTTg < 1:1540025/70‑1 (MQ=255)
|
GGACGATATTTTTCCGGCAGTTCTGGCACCGGACGCTTGTCATCGATGAGATGACGCACGGTTAAGATCGGATGACGCCACAGCATTCTCGGCCCGGCCCAACGCATAATCTGTTTCATCTCTTCACGCTTTG > W3110S.gb/2855128‑2855260
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
