Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F28 I1 R2	328	63.0	4687567	98.0%	4593815	65.2

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	2,906,931	A→G	V464V (GTT→GTC)	pyrG ←	CTP synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	2,906,931	0	A	G	100.0%	407.9 / NA	116	V464V (GTT→GTC)	pyrG	CTP synthetase
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (61/55); total (61/55)

Only 100 of 124 total aligned reads displayed.

CGCAGACCTGCATCTTCAATCTGTTTCAACAGCATGTTGTTGACTTCGTAACGGTGACGATGACGCTCAACAATTGTCGGCGCATTGTACAGCTGGCGAACCAGGCTATCGTCAACCAACTGGCACTGCTGT  >  W3110S.gb/2906863‑2906994

cgcAGACCTGCATCTTCAATCTGTTTCAACAGCATGTTGTTGACTTCGTAACGGTGACGATGACGCTcgac                                                               <  1:2703820/71‑1 (MQ=255)

 gcAGACCTGCATCTTCAATCTGTTTCAACAGCATGTTGTTGACTTCGTAACGGTGACGATGACGCTcgac                                                               <  1:13206/70‑1 (MQ=255)

  cAGACCTGCATCTTCAATCTGTTTCAACAGCATGTTGTTGACTTCGTAACGGTGACGATGACGCTcgacaa                                                             >  1:4416821/1‑71 (MQ=255)

    gACCTGCATCTTCAATCTGTTTCAACAGCATGTTGTTGACTTCGTAACGGTGACGATGACGCTCGACAAt                                                            >  1:2427871/1‑70 (MQ=255)

       cTGCATCTTCAATCTGTTTCAACAGCATGTTGTTGACTTCGTAACGGTGACGATGACGCTCGACAAtt                                                           <  1:1499469/68‑1 (MQ=255)

          cATCTTCAATCTGTTTCAACAGCATGTTGTTGACTTCGTAACGGTGACGATGACGCTCGACAATTGTCGgc                                                     >  1:4617439/1‑71 (MQ=255)

          cATCTTCAATCTGTTTCAACAGCATGTTGTTGACTTCGTAACGGTGACGATGACGCTCGACAATTGTCGgc                                                     >  1:3063674/1‑71 (MQ=255)

            tCTTCAATCTGTTTCAACAGCATGTTGTTGACTTCGTAACGGTGACGATGACGCTCGACAATTGTCGgcg                                                    <  1:372026/70‑1 (MQ=255)

            tCTTCAATCTGTTTCAACAGCATGTTGTTGACTTCGTAACGGTGACGATGACGCTCGACAATTGTCGgcg                                                    <  1:1597512/70‑1 (MQ=255)

              ttCAATCTGTTTCAACAGCATGTTGTTGACTTCGTAACGGTGACGATGACGCTcgacaa                                                             >  1:3062374/1‑59 (MQ=255)

              ttCAATCTGTTTCAACAGCATGTTGTTGACTTCGTAACGGTGACGATGACGCTcgacaa                                                             >  1:861575/1‑59 (MQ=255)

              ttCAATCTGTTTCAACAGCATGTTGTTGACTTCGTAACGGTGACGATGACGCTCGACAATTGTCGGCGCAt                                                 >  1:2271979/1‑71 (MQ=255)

              ttCAATCTGTTTCAACAGCATGTTGTTGACTTCGTAACGGTGACGATGACGCTCGACAATTGTCGGCGCAt                                                 >  1:3437335/1‑71 (MQ=255)

              ttCAATCTGTTTCAACAGCATGTTGTTGACTTCGTAACGGTGACGATGACGCTCGACAATTGTCGGCGCAt                                                 >  1:3115386/1‑71 (MQ=255)

              ttCAATCTGTTTCAACAGCATGTTGTTGACTTCGTAACGGTGACGATGACGCTCGACAATTGTCGGCGCAt                                                 >  1:3007418/1‑71 (MQ=255)

              ttCAATCTGTTTCAACAGCATGTTGTTGACTTCGTAACGGTGACGATGACGCTCGACAATTGTCGGCGCAt                                                 >  1:1084348/1‑71 (MQ=255)

              ttCAATCTGTTTCAACAGCATGTTGTTGACTTCGTAACGGTGACGATGACGCTCGACAATTGTCGGCGCAt                                                 >  1:2316017/1‑71 (MQ=255)

              ttCAATCTGTTTCAACAGCATGTTGTTGACTTCGTAACGGTGACGATGACGCTCGACAATTGTCGGCGCAt                                                 >  1:2426621/1‑71 (MQ=255)

              ttCAATCTGTTTCAACAGCATGTTGTTGACTTCGTAACGGTGACGATGACGCTCGACAATTGTCGGCGCAt                                                 >  1:2648560/1‑71 (MQ=255)

              ttCAATCTGTTTCAACAGCATGTTGTTGACTTCGTAACGGTGACGATGACGCTCGACAATTGTCGGCGCAt                                                 >  1:1793266/1‑71 (MQ=255)

              ttCAATCTGTTTCAACAGCATGTTGTTGACTTCGTAACGGTGACGATGACGCTCGACAATTGTCGGCGCAt                                                 >  1:40868/1‑71 (MQ=255)

              ttCAATCTGTTTCAACAGCATGTTGTTGACTTCGTAACGGTGACGATGACGCTCGACAATTGTCGGCGCAt                                                 >  1:4181003/1‑71 (MQ=255)

              ttCAATCTGTTTCAACAGCATGTTGTTGACTTCGTAACGGTGACGATGACGCTCGACAATTGTCGGCGCAt                                                 >  1:1523432/1‑71 (MQ=255)

              ttCAATCTGTTTCAACAGCATGTTGTTGACTTCGTAACGGTGACGATGACGCTCGACAATTGTCGGCGCAt                                                 >  1:1468266/1‑71 (MQ=255)

              ttCAATCTGTTTCAACAGCATGTTGTTGACTTCGTAACGGTGACGATGACGCTCGACAATTGTCGGCGCAt                                                 >  1:914055/1‑71 (MQ=255)

               tCAATCTGTTTCAACAGCATGTTGTTGACTTCGTAACGGTGACGATGACGCTCGACAATTGTCGGCGCAtt                                                >  1:2592347/1‑71 (MQ=255)

                cAATCTGTTTCAACAGCATGTTGTTGACTTCGTAACGGTGACGATGACGCTCGACAATTGTCgg                                                      >  1:2816209/1‑64 (MQ=255)

                cAATCTGTTTCAACAGCATGTTGTTGACTTCGTAACGGTGACGATGACGCTCGACAATTGTCGgcgc                                                   <  1:951308/67‑1 (MQ=255)

                cAATCTGTTTCAACAGCATGTTGTTGACTTCGTAACGGTGACGATGACGCTCGACAATTGTCGGCGCa                                                  <  1:3514152/68‑1 (MQ=255)

                 aaTCTGTTTCAACAGCATGTTGTTGACTTCGTAACGGTGACGATGACGCTCGACAATTGTCGGCGCATTg                                               <  1:4446476/70‑1 (MQ=255)

                 aaTCTGTTTCAACAGCATGTTGTTGACTTCGTAACGGTGACGATGACGCTCGACAATTGTCGGCGCATTg                                               <  1:1236951/70‑1 (MQ=255)

                  aTCTGTTTCAACAGCATGTTGTTGACTTCGTAACGGTGACGATGACGCTcgaca                                                              <  1:1753069/54‑1 (MQ=255)

                  aTCTGTTTCAACAGCATGTTGTTGACTTCGTAACGGTGACGATGACGCTCGACAATTGTCGGCGCATTg                                               <  1:2738237/69‑1 (MQ=255)

                         tCAACAGCATGTTGTTGACTTCGTAACGGTGACGATGACGCTCGACAATTGTCGGCGCATTGTACAGCTgg                                      >  1:4462540/1‑71 (MQ=255)

                         tCAACAGCATGTTGTTGACTTCGTAACGGTGACGATGACGCTCGACAATTGTCGGCGCATTGTACAGCTgg                                      >  1:3409664/1‑71 (MQ=255)

                         tCAACAGCATGTTGTTGACTTCGTAACGGTGACGATGACGCTCGACAATTGTCGGCGCATTGTACAGCTgg                                      >  1:3218172/1‑71 (MQ=255)

                           aaCAGCATGTTGTTGACTTCGTAACGGTGACGATGACGCTCGACAATTGTCGGCGCATTGTACAGCTGGCg                                    <  1:2165479/71‑1 (MQ=255)

                               gCATGTTGTTGACTTCGTAACGGTGACGATGACGCTCGACAATTGTCGGCGCATTGTACAGCTGGCGAAcc                                <  1:4445905/71‑1 (MQ=255)

                               gCATGTTGTTGACTTCGTAACGGTGACGATGACGCTCGACAATTGTCGGCGCATTGTACAGCTGGCGAAc                                 >  1:3489317/1‑70 (MQ=255)

                                 aTGTTGTTGACTTCGTAACGGTGACGATGACGCTCGACAATTGTCGGCGCATTGTACAGCTGGCGAACCAg                              <  1:4343044/71‑1 (MQ=255)

                                  tgttgtTGACTTCGTAACGGTGACGATGACGCTCGACAATTGTCGGCGCATTGTAc                                            <  1:3118662/56‑1 (MQ=255)

                                  tgttgtTGACTTCGTAACGGTGACGATGACGCTCGACAATTGTCGGCGCATTGTAc                                            <  1:2965043/56‑1 (MQ=255)

                                  tgttgtTGACTTCGTAACGGTGACGATGACGCTCGACAATTGTCGGCGCATTGTACAGCTGGCGAAcc                                <  1:734766/68‑1 (MQ=255)

                                    ttgttgACTTCGTAACGGTGACGATGACGCTCGACAATTGTCGGCGCAtt                                                >  1:626598/1‑50 (MQ=255)

                                    ttgttgACTTCGTAACGGTGACGATGACGCTCGACAATTGTCGGCGCATTGTAc                                            >  1:2484967/1‑54 (MQ=255)

                                    ttgttgACTTCGTAACGGTGACGATGACGCTCGACAATTGTCGGCGCATTGTACAg                                          >  1:4172456/1‑56 (MQ=255)

                                    ttgttgACTTCGTAACGGTGACGATGACGCTCGACAATTGTCGGCGCATTGTACAGc                                         >  1:3028658/1‑57 (MQ=255)

                                    ttgttgACTTCGTAACGGTGACGATGACGCTCGACAATTGTCGGCGCATTGTACAGCTGGc                                     >  1:1562439/1‑61 (MQ=255)

                                    ttgttgACTTCGTAACGGTGACGATGACGCTCGACAATTGTCGGCGCATTGTACAGCTGGCGAAc                                 >  1:2070271/1‑65 (MQ=255)

                                    ttgttgACTTCGTAACGGTGACGATGACGCTCGACAATTGTCGGCGCATTGTACAGCTGGCGAACCAgg                             >  1:3311322/1‑69 (MQ=255)

                                    ttgttgACTTCGTAACGGTGACGATGACGCTCGACAATTGTCGGCGCATTGTACAGCTGGCGAACCAGGc                            >  1:297743/1‑70 (MQ=255)

                                    ttgttgACTTCGTAACGGTGACGATGACGCTCGACAATTGTCGGCGCATTGTACAGCTGGCGAACCAGGc                            >  1:2534628/1‑70 (MQ=255)

                                    ttgttgACTTCGTAACGGTGACGATGACGCTCGACAATTGTCGGCGCATTGTACAGCTGGCGAACCAGGc                            >  1:3932197/1‑70 (MQ=255)

                                    ttgttgACTTCGTAACGGTGACGATGACGCTCGACAATTGTCGGCGCATTGTACAGCTGGCGAACCAGGc                            >  1:3918000/1‑70 (MQ=255)

                                    ttgttgACTTCGTAACGGTGACGATGACGCTCGACAATTGTCGGCGCATTGTACAGCTGGCGAACCAGGc                            >  1:3843280/1‑70 (MQ=255)

                                    ttgttgACTTCGTAACGGTGACGATGACGCTCGACAATTGTCGGCGCATTGTACAGCTGGCGAACCAGGc                            <  1:787662/70‑1 (MQ=255)

                                    ttgttgACTTCGTAACGGTGACGATGACGCTCGACAATTGTCGGCGCATTGTACAGCTGGCGAACCAGGc                            >  1:3659727/1‑70 (MQ=255)

                                    ttgttgACTTCGTAACGGTGACGATGACGCTCGACAATTGTCGGCGCATTGTACAGCTGGCGAACCAGGc                            >  1:3659393/1‑70 (MQ=255)

                                    ttgttgACTTCGTAACGGTGACGATGACGCTCGACAATTGTCGGCGCATTGTACAGCTGGCGAACCAGGc                            >  1:3601768/1‑70 (MQ=255)

                                    ttgttgACTTCGTAACGGTGACGATGACGCTCGACAATTGTCGGCGCATTGTACAGCTGGCGAACCAGGc                            >  1:1919012/1‑70 (MQ=255)

                                    ttgttgACTTCGTAACGGTGACGATGACGCTCGACAATTGTCGGCGCATTGTACAGCTGGCGAACCAGGc                            >  1:2812084/1‑70 (MQ=255)

                                    ttgttgACTTCGTAACGGTGACGATGACGCTCGACAATTGTCGGCGCATTGTACAGCTGGCGAACCAGGc                            >  1:648243/1‑70 (MQ=255)

                                    ttgttgACTTCGTAACGGTGACGATGACGCTCGACAATTGTCGGCGCATTGTACAGCTGGCGAACCAGGc                            >  1:1010021/1‑70 (MQ=255)

                                    ttgttgACTTCGTAACGGTGACGATGACGCTCGACAATTGTCGGCGCATTGTACAGCTGGCGAACCAGGCt                           <  1:691865/71‑1 (MQ=255)

                                    ttgttgACTTCGTAACGGTGACGATGACGCTCGACAATTGTCGGCGCATTGTACAGCTGGCGAACCAGGCt                           <  1:148740/71‑1 (MQ=255)

                                    ttgttgACTTCGTAACGGTGACGATGACGCTCGACAATTGTCGGCGCATTGTACAGCTGGCGAACCAGGCt                           <  1:2681018/71‑1 (MQ=255)

                                    ttgttgACTTCGTAACGGTGACGATGACGCTCGACAATTGTCGGCGCATTGTACAGCTGGCGAACCAGGCt                           <  1:2136809/71‑1 (MQ=255)

                                      gttgACTTCGTAACGGTGACGATGACGCTCGACAATTGTCGGCGCATTGTACAGCTGGCGAACCAGGCTa                          <  1:4253145/70‑1 (MQ=255)

                                      gttgACTTCATAACGGTGACGATGACGCTCGACAATTGTCGGCGCATTGTACAGCTGGCGAACCAGGCTa                          <  1:4145458/70‑1 (MQ=255)

                                         gACTTCGTAACGGTGACGATGACGCTCGACAATTGTCGGCGCATTGTACAGCTGGCGAACCAGGCTATCGt                      >  1:589213/1‑71 (MQ=255)

                                          aCTTCGTAACGGTGACGATGACGCTCGACAATTGTCGGCGCATTGTACAGCTGGCGAACCAGGCTATCGt                      >  1:172201/1‑70 (MQ=255)

                                          aCTTCGTAACGGTGACGATGACGCTCGACAATTGTCGGCGCATTGTACAGCTGGCGAACCAGGCTATCGTc                     <  1:3354084/71‑1 (MQ=255)

                                            ttCGTAACGGTGACGATGACGCTCGACAATTGTCGGCGCATTGTACAGCTGGCGAACCAGGCTATCGTc                     <  1:4631527/69‑1 (MQ=255)

                                              cGTAACGGTGACGATGACGCTCGACAATTGTCGGCGCATTGTACAGCTGGCGAACCAGGCTATCGTcaa                   >  1:2931459/1‑69 (MQ=255)

                                               gTAACGGTGACGATGACGCTCGACAATTGTCGGCGCATTGTACAGCTGGCGAACCAGGCTATCGTcaacca                <  1:4630947/71‑1 (MQ=255)

                                               gTAACGGTGACGATGACGCTCGACAATTGTCGGCGCATTGTACAGCTGGCGAACCAGGCTATCGTcaacca                <  1:1537405/71‑1 (MQ=255)

                                               gTAACGGTGACGATGACGCTCGACAATTGTCGGCGCATTGTACAGCTGGCGAACCAGGCTATCGTcaacc                 <  1:652477/70‑1 (MQ=255)

                                               gTAACGGTGACGATGACGCTCGACAATTGTCGGCGCATTGTACAGCTGGCGAACCAGGCTATCGTcaacc                 <  1:3105533/70‑1 (MQ=255)

                                               gTAACGGTGACGATGACGCTCGACAATTGTCGGCGCATTGTACAGCTGGCGAACCAGGCTATCGTcaacc                 <  1:4672543/70‑1 (MQ=255)

                                               gTAACGGTGACGATGACGCTCGACAATTGTCGGCGCATTGTACAGCTGGCGAACCAGGCTATCGTcaacc                 <  1:1435908/70‑1 (MQ=255)

                                               gTAACGGTGACGATGACGCTCGACAATTGTCGGCGCATTGTACAGCTGGCGAACCAGGCTATCGTcaacc                 <  1:3588703/70‑1 (MQ=255)

                                               gTAACGGTGACGATGACGCTCGACAATTGTCGGCGCATTGTACAGCTGGCGAACCAGGCTATCGTcaacc                 <  1:1943205/70‑1 (MQ=255)

                                               gTAACGGTGACGATGACGCTCGACAATTGTCGGCGCATTGTACAGCTGGCGAACCAGGATATCGTcaacca                <  1:4273269/71‑1 (MQ=255)

                                                    ggTGACGATGACGCTCGACAATTGTCGGCGCATTGTACAGCTGGCGAACCAGGCTATCGTCAACCAACTgg           <  1:4247360/71‑1 (MQ=255)

                                                    ggTGACGATGACGCTCGACAATTGTCGGCGCATTGTACAGCTGGCGAACCAGGCTATCGTCAACCAACTgg           <  1:957271/71‑1 (MQ=255)

                                                    ggTGACGATGACGCTCGACAATTGTCGGCGCATTGTACAGCTGGCGAACCAGGCTATCGTCAACCAACTgg           <  1:1110724/71‑1 (MQ=255)

                                                    ggTGACGATGACGCTCGACAATTGTCGGCGCATTGTACAGCTGGCGAACCAGGCTATCGTCAACCAACTgg           <  1:1591428/71‑1 (MQ=255)

                                                    ggTGACGATGACGCTCGACAATTGTCGGCGCATTGTACAGCTGGCGAACCAGGCTATCGTCAACCAACTgg           <  1:1815885/71‑1 (MQ=255)

                                                    ggTGACGATGACGCTCGACAATTGTCGGCGCATTGTACAGCTGGCGAACCAGGCTATCGTCAACCAACTgg           <  1:2141061/71‑1 (MQ=255)

                                                    ggTGACGATGACGCTCGACAATTGTCGGCGCATTGTACAGCTGGCGAACCAGGCTATCGTCAACCAACTgg           <  1:272203/71‑1 (MQ=255)

                                                    ggTGACGATGACGCTCGACAATTGTCGGCGCATTGTACAGCTGGCGAACCAGGCTATCGTCAACCAACTgg           <  1:2959213/71‑1 (MQ=255)

                                                    ggTGACGATGACGCTCGACAATTGTCGGCGCATTGTACAGCTGGCGAACCAGGCTATCGTCAACCAACTgg           <  1:3184527/71‑1 (MQ=255)

                                                    ggTGACGATGACGCTCGACAATTGTCGGCGCATTGTACAGCTGGCGAACCAGGCTATCGTCAACCAACTgg           <  1:4138652/71‑1 (MQ=255)

                                                     gTGACGATGACGCTCGACAATTGTCGGCGCATTGTACAGCTGGCGAACCAGGCTATCGTCAACCAACTgg           >  1:750972/1‑70 (MQ=255)

                                                     gTGACGATGACGCTCGACAATTGTCGGCGCATTGTACAGCTGGCGAACCAGGCTATCGTCAACCAACTGGc          <  1:23666/71‑1 (MQ=255)

                                                     gTGACGATGACGCTCGACAATTGTCGGCGCATTGTACAGCTGGCGAACCAGGCTATCGTCAACCAACTGGc          <  1:2735377/71‑1 (MQ=255)

                                                          gATGACGCTCGACAATTGTCGGCGCATTGTACAGCTGGCGAACCAGGCTATCGTCAACCAACTGGCActgc     >  1:4240290/1‑71 (MQ=255)

                                                             gACGCTCGACAATTGTCGGCGCATTGTACAGCTGGCGAACCAGGCTATCGTcaaccaa               >  1:4050510/1‑58 (MQ=255)

                                                             gACGCTCGACAATTGTCGGCGCATTGTACAGCTGGCGAACCAGGCTATCGTCAACCAACTGGCACTGCtgt  >  1:1887469/1‑71 (MQ=255)

                                                               cGCTCGTCAATTGTCGGCGCATTGTACAGCTGGCGAACCAGGCTATc                        <  1:2500534/47‑1 (MQ=39)

CGCAGACCTGCATCTTCAATCTGTTTCAACAGCATGTTGTTGACTTCGTAACGGTGACGATGACGCTCAACAATTGTCGGCGCATTGTACAGCTGGCGAACCAGGCTATCGTCAACCAACTGGCACTGCTGT  >  W3110S.gb/2906863‑2906994

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A