Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F17 I0 R2
|
276 |
45.3 |
3729697 |
93.0% |
3468618 |
60.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
W3110S.gb |
2,906,931 |
A→G |
46.4% |
V464V (GTT→GTC) |
pyrG ← |
CTP synthetase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 2,906,931 | 0 | A | G | 46.4%
| 7.7
/ 99.8
| 69 | V464V (GTT→GTC) | pyrG | CTP synthetase |
| Reads supporting (aligned to +/- strand): ref base A (11/26); new base G (10/22); total (21/48) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.68e-01 |
CAGACCTGCATCTTCAATCTGTTTCAACAGCATGTTGTTGACTTCGTAACGGTGACGATGACGCTCAACAATTGTCGGCGCATTGTACAGCTGGCGAACCAGGCTATCGTCAACCAACTGGC > W3110S.gb/2906865‑2906986
|
cAGACCTGCATCTTCAATCTGTTTCAACAGCATGTTGTTGACTTCGTAACGGTGACGATGACGCTcaac < 1:1215431/69‑1 (MQ=255)
cAGACCTGCATCTTCAATCTGTTTCAACAGCATGTTGTTGACTTCGTAACGGTGACGATGACGCTcaac < 1:2487204/69‑1 (MQ=255)
cAGACCTGCATCTTCAATCTGTTTCAACAGCATGTTGTTGACTTCGTAACGGTGACGATGACGCTcaac < 1:2647252/69‑1 (MQ=255)
ccTGCATCTTCAATCTGTTTCAACAGCATGTTGTTGACTTCGTAACGGTGACTATGACGCTCGACAAtt > 1:550081/1‑69 (MQ=255)
gCATCTTCAATCTGTTTCAACAGCATGTTGTTGACTTCGTAACGGTGACGATGACGCTcaac < 1:1222967/62‑1 (MQ=255)
gCATCTTCAATCTGTTTCAACAGCATGTTGTTGACTTCGTAACGGTGACGATGACGCTcaac < 1:1478780/62‑1 (MQ=255)
gCATCTTCAATCTGTTTCAACAGCATGTTGTTGACTTCGTAAAGGTGACGATGACGCTCAACAATTGTc < 1:3346040/69‑1 (MQ=255)
cATCTTCAATCTGTTTCAACAGCATGTTGTTGACTTCGTAACGGTGACGATGACGCTCGACAATTGTCg > 1:658018/1‑69 (MQ=255)
cATCTTCAATCTGTTTCAACAGCATGTTGTTGACTTCGTAACGGTGACGATGACGCTCGACAATTGTCg > 1:568959/1‑69 (MQ=255)
cATCTTCAATCTGTTTCAACAGCATGTTGTTGACTTCGTAACGGTGACGATGACGCTCGACAATTGTCg > 1:3728980/1‑69 (MQ=255)
ttCAATCTGTTTCAACAGCATGTTGTTGACTTCGTAACGGTGACGATGACGCTCGACAATTGTCGgcgc > 1:2405572/1‑69 (MQ=255)
ttCAATCTGTTTCAACAGCATGTTGTTGACTTCGTAACGGTGACGATGACGCTCAACAAtt > 1:543367/1‑61 (MQ=255)
cAATCTGTTTCAACAGCATGTTGTTGACTTCGTAACGGTGACGATGACGCTCGACAATTGTCGGCGCAt > 1:3090298/1‑69 (MQ=255)
cAATCTGTTTCAACAGCATGTTGTTGACTTCGTAACGGTGACGATGACGCTCGACAATTGTCGGCGCAt > 1:2680137/1‑69 (MQ=255)
cAATCTGTTTCAACAGCATGTTGTTGACTTCGTAACGGTGACGATGACGCTCAACAATTGTCGGCGCAt > 1:17006/1‑69 (MQ=255)
cAATCTGTTTCAACAGCATGTTGTTGACTTCGTAACGGTGACGATGACGCTCAACAATTGTCGGCGCAt > 1:1572124/1‑69 (MQ=255)
tCTGTTTCAACAGCATGTTGTTGACTTCGTAACGGTGCCGATGACGCTCAACAATTGTCGGCGCATTg > 1:1794629/1‑68 (MQ=255)
tCTGTTTCAACAGCATGTTGTTGACTTCGTAACGGTGACGATGACGCTCGACAATTGTCGGCGCATTg < 1:2669027/68‑1 (MQ=255)
tCTGTTTCAACAGCATGTTGTTGACTTCGTAACGGTGACGATGACGCTCGACAATTGTCGGCGCATTg < 1:1770018/68‑1 (MQ=255)
tCTGTTTCAACAGCATGTTGTTGACTTCGTAACGGTGACGATGACGCTCGACAATTGTCGGCGCATTg < 1:2664846/68‑1 (MQ=255)
tCTGTTTCAACAGCATGTTGTTGACTTCGTAACGGTGACGATGACGCTCGACAATTGTCGGCGCATTg < 1:1974768/68‑1 (MQ=255)
tCTGTTTCAACAGCATGTTGTTGACTTCGTAACGGTGACGATGACGCTCGACAATTGTCGGCGCATTg < 1:202245/68‑1 (MQ=255)
tCTGTTTCAACAGCATGTTGTTGACTTCGTAACGGTGACGATGACGCTCGACAATTGTCGGCGCATTg < 1:2536395/68‑1 (MQ=255)
tCTGTTTCAACAGCATGTTGTTGACTTCGTAACGGTGACGATGACGCTCAACAATTGTCGGCGCATTg > 1:1798907/1‑68 (MQ=255)
tCTGTTTCAACAGCATGTTGTTGACTTCGTAACGGTGACGATGACGCTCAACAATTGTCGGCGCATTg > 1:3021133/1‑68 (MQ=255)
tCAAAAGCATGTTGTTGACTTCGTAACGGTGACGATGACGCTCAACAATTGTCGGCGCATTGTACAGCt > 1:2310828/1‑69 (MQ=255)
cAGCATGTTGTTGACTTCGTAACGGTGACGATGACGCTCAACAATTGTCGGCGCATTGTACAGCTGGCg < 1:2621507/69‑1 (MQ=255)
cAGCATGTTGTTGACTTCGTAACGGTGACGATGACGCTCAACAATTGTCGGCGCATTGTACAGCTGGCg < 1:201223/69‑1 (MQ=255)
cAGCATGTTGTTGACTTCGTAACGGTGACGATGACGCTCAACAATTGTCGGCGCATTGTACAGCTGGCg < 1:3683997/69‑1 (MQ=255)
cATGTTGTTGACTTCGTAACGGTGACGATGACGCTCAACAATTGTCGGCGCa > 1:104559/1‑52 (MQ=255)
cATGTTGTTGACTTCGTAACGGTGACGATGACGCTCAACAATTGTCGGCGCATTg < 1:3339306/55‑1 (MQ=255)
cATGTTGTTGACTTCGTAACGGTGACGATGACGCTCAACAATTGTCGGCGCATTg < 1:3195041/55‑1 (MQ=255)
cATGTTGTTGACTTCGTAACGGTGACGATGACGCTCAACAATTGTCGGCGCATTg < 1:2750130/55‑1 (MQ=255)
cATGTTGTTGACTTCGTAACGGTGACGATGACGCTCAACAATTGTCGGCGCATTg < 1:1158803/55‑1 (MQ=255)
cATGTTGTTGACTTCGTAACGGTGACGATGACGCTCAACAATTGTCGGCGCATTGTACAGCTGGCGAAc < 1:2313936/69‑1 (MQ=255)
aTGTTGTTGACTTCGTAACGGTGACGATGACGCTCAACAATTGTCGGCGCATTGTACAGCTGGCGAAcc < 1:1884411/69‑1 (MQ=255)
tgttgtTGACTTCGTAACGGTGACGATGACGCTCAACAATTGTCGGCGCATTGTACAGCTGGCg < 1:1477852/64‑1 (MQ=255)
ttgttgACTTCGTAACGGTGACGATGACGCTCGACAATTGTCGGCGCATTg < 1:1752037/51‑1 (MQ=255)
ttgttgACTTCGTAACGGTGACGATGACGCTCGACAATTGTCGGCGCATTg < 1:54256/51‑1 (MQ=255)
ttgttgACTTCGTAACGGTGACGATGACGCTCGACAATTGTCGGCGCATTg < 1:1578265/51‑1 (MQ=255)
ttgttgACTTCGTAACGGTGACGATGACGCTCGACAATTGTCGGCGCATTg < 1:3656284/51‑1 (MQ=255)
ttgttgACTTCGTAACGGTGACGATGACGCTCGACAATTGTCGGCGCATTg < 1:1260157/51‑1 (MQ=255)
ttgttgACTTCGTAACGGTGACGATGACGCTCGACAATTGTCGGCGCATTg < 1:2343887/51‑1 (MQ=255)
tgttgACTTCGTAACGGTGACGATGACGCTCGACAATTGTCGGCGCATTg < 1:1064808/50‑1 (MQ=255)
tgttgACTTCGTAACGGTGACGATGACGCTCGACAATTGTCGGCGCATTg < 1:3339532/50‑1 (MQ=255)
tgttgACTTCGTAACGGTGACGATGACGCTCGACAATTGTCGGCGCATTg < 1:2252189/50‑1 (MQ=255)
gttgACTTCGTAACGGTGACGATGACGCTCGACAATTGTCGGCGCATTGTACAGCTGGCGAACCAGGCt > 1:2066177/1‑69 (MQ=255)
tgACTTCGTAACGGTGACGATGACGCTCAACAAtt < 1:2040076/35‑1 (MQ=255)
tgACTTCGTAACGGTGACGATGACGCTCAACAAtt < 1:3474982/35‑1 (MQ=255)
tgACTTCGTAACGGTGACGATGACGCTCAACAAtt < 1:3287464/35‑1 (MQ=255)
tgACTTCGTAACGGTGACGATGACGCTCAACAAtt < 1:3067957/35‑1 (MQ=255)
tgACTTCGTAACGGTGACGATGACGCTCAACAAtt > 1:2893175/1‑35 (MQ=255)
tgACTTCGTAACGGTGACGATGACGCTCAACAAtt < 1:37589/35‑1 (MQ=255)
tgACTTCGTAACGGTGACGATGACGCTCAACAAtt < 1:677311/35‑1 (MQ=255)
tgACTTCGTAACGGTGACGATGACGCTCAACAAtt < 1:1938230/35‑1 (MQ=255)
ttCGTAACGGTGACGATGACGCTCAACAATTGTCTGCGCATTGTACAGCTGGCGAACCAGGCTATCGTc < 1:79684/69‑1 (MQ=255)
tCGTAACGGTGACGATGACGCTCAACAATTGTCGGCGCATTGTACAGCTGGCGAACCAGGCTATCGTca > 1:663170/1‑69 (MQ=255)
tCGTAACGGTGACGATGACGCTCAACAATTGTCGGCGCATTGTACAGCTGGCGAACCACGCTATCGTc > 1:1039172/1‑68 (MQ=255)
aaCGGTGACGATGACGCTCAACAATTGTCGGCGCATTGTACAGCTGGCGAACCAGGCTATCGTcaacc < 1:1883982/68‑1 (MQ=255)
aaCGGTGACGATGACGCTAGACAATTGTCGGCGCATTGTACAGCTGGCGAACCAGGCTATCGTcaacca < 1:2076269/69‑1 (MQ=255)
aCGGTGACGATGACGCTCAACAATTGTCGGCGCATTGTACAGCTGGCGAACCAGGCTATCGTcaacca < 1:2677085/68‑1 (MQ=255)
tGACGATGACGCTCGACAATTGTCGGCGCATTGTACAGCTGGCGAACCAGGCTATCGTCAACCAACTgg < 1:3225986/69‑1 (MQ=255)
tGACGATGACGCTCGACAATTGTCGGCGCATTGTACAGCTGGCGAACCAGGCTATCGTCAACCAACTgg < 1:2867192/69‑1 (MQ=255)
tGACGATGACGCTCAACAATTGTCGGCGCATTGTACAGCTGGCGAACCAGGCTATCGTCAACCAACTgg < 1:39067/69‑1 (MQ=255)
gaggATGACGCTCAACAATTGTCGGCGCATTGTACAGCTGGCGAACCAGGCTATCGTCAACCAACTGGc < 1:1508185/66‑1 (MQ=255)
gACGATGACGCTCGACATTTGTCGGCGCATTGTACAGCTGGCGAACCAGGCTATCGTCAACGAACTgg < 1:479103/68‑1 (MQ=255)
gACGATGACGCTCGACAATTGTCGGCGCATTGTACAGCTGGCGAACCAGGCTATCGTCAACCAACTgg < 1:1395600/68‑1 (MQ=255)
gACGATGACGCTCAACAATTGTCGGCGCATTGTACAGCTGGCGAACCAGGCTATCGTCAACCAACTGGc < 1:423836/69‑1 (MQ=255)
gACGATGACGCTCAACAATTGTCGGCGCATTGTACAGCTGGCGAACCAGGCTATCGTCAACCAACTGGc < 1:1061089/69‑1 (MQ=255)
gATGACGCTCAACAATTGTCGGCGCATTGTACAGCTGGCGAACCAGGCTATCGTCAACCAACTgg < 1:2126421/65‑1 (MQ=255)
cGCTCGACAATTGTCGGCGCATTGTACAGCTGGCGAACCAGGCTATCGTCAACCAACTgg > 1:1610611/1‑60 (MQ=255)
cGCTCGACAATTGTCGGCGCATTGTACAGCTGGCGAACCAGGCTATCGTCAACCAACTgg > 1:567332/1‑60 (MQ=255)
cGCTCGACAATTGTCGGCGCATTGTACAGCTGGCGAACCAGGCTATCGTCAACCAACTgg < 1:3342341/60‑1 (MQ=255)
cGCTCGACAATTGTCGGCGCATTGTACAGCTGGCGAACCAGGCTATCGTCAACCAACTgg < 1:3591259/60‑1 (MQ=255)
|
CAGACCTGCATCTTCAATCTGTTTCAACAGCATGTTGTTGACTTCGTAACGGTGACGATGACGCTCAACAATTGTCGGCGCATTGTACAGCTGGCGAACCAGGCTATCGTCAACCAACTGGC > W3110S.gb/2906865‑2906986
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
