Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F28 I1 R2
|
328 |
63.0 |
4687567 |
98.0% |
4593815 |
65.2 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
2,971,531 |
G→T |
L330I (CTA→ATA) |
ygeD ← |
predicted inner membrane protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 2,971,531 | 0 | G | T | 100.0%
| 64.2
/ NA
| 24 | L330I (CTA→ATA) | ygeD | predicted inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (4/20); total (4/20) |
TCGCCAAGGTTTTGTACTGCAATCGCATTCCCCGCCCCGACGCTTTTTTTACCCCGCTCCTGTAGCAACGCATTGAGCGGAACGACAAAAAAGCCCCCCATCACGCCAATCAGCATCAACAAGGCA > W3110S.gb/2971467‑2971592
|
tCGCCAAGGTTTTGTACTGCAATCGCATTCCCCGCCCCGACGCTTTTTTTACCCCGCTCCTGTATCAACg < 1:2067181/70‑1 (MQ=255)
cGCCAAGGTTTTGTACTGCAATCGCATTCCCCGCGCCGACGCTTTTTTTACCCCGCTCCTGTATCAACGc < 1:2306240/70‑1 (MQ=255)
cGCCAAGGTTTTGTACTGCAATCGCATTCCCCGCCCCGACGCTTTTTTTAGCCCGCTCCTGTATCAACGc < 1:911725/70‑1 (MQ=255)
gCCAAGGTTTTGTACTGCAATCGCATTCCCCGCCCCGACGCTTTTTTTACCCCGCTCCTGTATCAACGCa < 1:1960022/70‑1 (MQ=255)
gCCAAGGTTTTGTACTGCAATCGCATTCCCCGCCCCGACGCTTTTTTTACCCCGCTCCTGTATCAACGCa < 1:1631286/70‑1 (MQ=255)
ttttGTACTGCAATCGCATTCCCCGCCCCGACGCTTTTTTTACCCCGCTCCTGTATCAACGCATTGAGCgg < 1:1149325/71‑1 (MQ=255)
gCAATCGCATTCCCCGCCCCGACGCTTTTTTTACCCCGCTCCTGTATCAACGCATTGAGCGGAACGACaa > 1:2764198/1‑70 (MQ=255)
cAATCGCATTCCCCGCCCCGACGCTTTTTTTACCCCGCTCCTGTATCAACGCATTGAGCGGAACGACaaaa < 1:2331996/71‑1 (MQ=255)
aaTCGCATTCCCCGCCCCGACGCTTTTTTTACCCCGCTCCTGTATCAACGCATTGAGCGGAACGACaaaaa < 1:119159/71‑1 (MQ=255)
aTCGCATTCCCCGCCCCGACGCTTTTTTTACCCCGCTCCTGTATCAACGCATTGAGCGGAACGACaaaaaa < 1:1457095/71‑1 (MQ=255)
aTCGCATTCCCCGCCCCGACGCTTTTTTTACCCCGCTCCTGTATCAACGCATTGAGCGGAACGACaaaaaa < 1:2741971/71‑1 (MQ=255)
tCGCATTCCCCGCCCCGACGCTTTTTTTACCCCGCTCCTGTATCAACg < 1:2769987/48‑1 (MQ=255)
ttCCCCGCCCCGACGCTTTTTTTACCCCGCTCCTGTATCaa > 1:1242008/1‑41 (MQ=255)
ttCCCCGCCCCGACGCTTTTTTTACCCCGCTCCTGTATCAACGCATTGAGCGGAACGACAAAAAAGccccc < 1:1536824/71‑1 (MQ=255)
cccgccccgACGCTTTTTTTACCCCGCTCCTGTATCAACGCATTGAGCGGAACGACAAAAAAGCCCCCCAt < 1:900301/71‑1 (MQ=255)
cTTTTTTTACCCCGCTCCTGTATCAACGCATTGAGCGGAACGACAAAAAAGCCCCCCATCACGCCAATCAg < 1:2157892/71‑1 (MQ=255)
ccccTCCTGTATCAACGCATTGAGCGGAACGACAAAAAAGCCCCCCATCACGCCAATCAGCATCAAAAAgg < 1:4286269/68‑1 (MQ=255)
ccGCTCCTGTATCAACGCATTGAGCGGAACGACAAAAAAGCCCCCCATCACGCCAATCAGCATCAACAAgg < 1:341192/71‑1 (MQ=255)
ccGCTCCTGTATCAACGCATTGAGCGGAACGACAAAAAAGCCCCCCATCACGCCAATCAGCATCAACAAgg < 1:4397059/71‑1 (MQ=255)
ccGCTCCTGTATCAACGCATTGAGCGGAACGACAAAAAAGCCCCCCATCACGCCAATCAGCATCAACAAgg < 1:1081529/71‑1 (MQ=255)
cGCTCCTGTATCAACGCATTGAGCGGAACGACAAAAAAGCCCCCCATCACGCCAATCAGCATCAACAAgg < 1:1072290/70‑1 (MQ=255)
cGCTCCTGTATCAACGCATTGAGCGGAACGACAAAAAAGCCCCCCATCACGCCAATCAGCATCAACAAgg > 1:4501770/1‑70 (MQ=255)
gCTCCTGTATCAACGCATTGAGCGGAACGACAAAAAAGCCCCCCATCACGCCAATCAGCATCAACAAGGCa < 1:3168195/71‑1 (MQ=255)
ccTGTATCAACGCATTGAGCGGAACGACAAAAAAGCCCCCCATCAc > 1:3721801/1‑46 (MQ=255)
|
TCGCCAAGGTTTTGTACTGCAATCGCATTCCCCGCCCCGACGCTTTTTTTACCCCGCTCCTGTAGCAACGCATTGAGCGGAACGACAAAAAAGCCCCCCATCACGCCAATCAGCATCAACAAGGCA > W3110S.gb/2971467‑2971592
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
