Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F28 I1 R2
|
328 |
63.0 |
4687567 |
98.0% |
4593815 |
65.2 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
3,219,258 |
T→C |
F379S (TTC→TCC) |
ygjG → |
putrescine:2‑oxoglutaric acid aminotransferase, PLP‑dependent |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 3,219,258 | 0 | T | C | 99.0%
| 331.9
/ ‑4.5
| 97 | F379S (TTC→TCC) | ygjG | putrescine:2‑oxoglutaric acid aminotransferase, PLP‑dependent |
| Reads supporting (aligned to +/- strand): ref base T (0/1); new base C (39/57); total (39/58) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01 |
AATGTGTTGCTGGAGCAGAACTTACCGGCTCAGGCTGAGCAAAAAGGCGATATGTTGCTGGACGGTTTCCGTCAACTGGCGCGGGAATATCCCGATCTGGTACAGGAAGCGCGTGGTAAAGGGATGTTGA > W3110S.gb/3219191‑3219320
|
aaTGTGTTGCTGGAGCAGAACTTACCGGCTCAGGCTGAGCAAAAAGGCGATATGTTGCTGGACGGTTccc > 1:752672/1‑70 (MQ=255)
tgtgTTGCTGGAGCAGAACTTACCGGCTCAGGCTGAGCAAAAAGGCGATATGTTGCTGGACGGTTCCCGt > 1:935013/1‑70 (MQ=255)
tgtgTTGCTGGAGCAGAACTTACCGGCTCAGGCTGAGCAAAAAGGCGATATGTTGCTGGACGGTTCCCGt > 1:4180976/1‑70 (MQ=255)
tgtgTTGCTGGAGCAGAACTTACCGGCTCAGGCTGAGCAAAAAGGCGATATGTTGCTGGACGGTTCCCGTc < 1:3363631/71‑1 (MQ=255)
tgtgTTGCTGGAGCAGAACTTACCGGCTCAGGCTGAGCAAAAAGGCGATATGTTGCTGGACGGTTCCCGTc < 1:4293488/71‑1 (MQ=255)
tgtgTTGCTGGAGCAGAACTTACCGGCTCAGGCTGAGCAAAAAGGCGATATGTTGCTGGACGGTTCCCGTc < 1:8674/71‑1 (MQ=255)
tgtTGCTGGAGCAGAACTTACCGGCTCAGGCTGAGCAAAAAGGCGATATGTTGCTGGACGGTTCCCGTCaa > 1:34214/1‑71 (MQ=255)
tgtTGCTGGAGCAGAACTTACCGGCTCAGGCTGAGCAAAAAGGCGATATGTTGCTGGACGGTTCCCGTCaa > 1:2862512/1‑71 (MQ=255)
tgtTGCTGGAGCAGAACTTACCGGCTCAGGCTGAGCAAAAAGGCGATATGTTGCTGGACGGTTCCCGTCaa > 1:1999664/1‑71 (MQ=255)
tgtTGCTGGAGCAGAACTTACCGGCTCAGGCTGAGCAAAAAGGCGATATGTTGCTGGACGGTTCCCGTCaa > 1:1263224/1‑71 (MQ=255)
tgtTGCTGGAGCAGAACTTACCGGCTCAGGCTGAGCAAAAAGGCGATATGTTGCTGGACGGTTCCCGTCaa > 1:1627342/1‑71 (MQ=255)
tgtTGCTGGAGCAGAACTTACCGGCTCAGGCTGAGCAAAAAGGCGATATGTTGCTGGACGGTTCCCGTCa < 1:2665596/70‑1 (MQ=255)
tgtTGCTGGAGCAGAACTTACCGGCTCAGGCTGAGCAAAAAGGCGATATGTTGCTGGACGGTTCCCGTCa < 1:3144017/70‑1 (MQ=255)
tgtTGCTGGAGCAGAACTTACCGGCTCAGGCTGAGCAAAAAGGCGATATGTTCCTGGACGGTTCCCGTCa < 1:868113/70‑1 (MQ=255)
gCTGGAGCAGAACTTACCGGCTCAGGCTGAGCAAAAAGGCGATATGTTGCTGGACGGTTCCCGTCAACTgg > 1:2342953/1‑71 (MQ=255)
gCAGAACTTACCGGCTCAGGCTGAGCAAAAAGGCGATATGTTGCTGGACGGTTCCCGTCaa > 1:1950171/1‑61 (MQ=255)
gCAGAACTTACCGGCTCAGGCTGAGCAAAAAGGCGATATGTTGCTGGACGGTTCCCGTCaa > 1:237750/1‑61 (MQ=255)
gCAGAACTTACCGGCTCAGGCTGAGCAAAAAGGCGATATGTTGCTGGACGGTTCCCGTCAACTGGCGCggg < 1:3943772/71‑1 (MQ=255)
gCAGAACTTACCGGCTCAGGCTGAGCAAAAAGGCGATATGTTGCTGGACGGTTCCCGTCAACTGGCGCggg < 1:488239/71‑1 (MQ=255)
cAGAACTTACCGGCTCAGGCTGAGCAAAAAGGCGATATGTTGCTGGACGGTTCCCGTCAACTGGCGCggg < 1:3398545/70‑1 (MQ=255)
cAGAACTTACCGGCTCAGGCTGAGCAAAAAGGCGATATGTTGCTGGACGG‑TTCCGTCAACTGGCGCGGGaa < 1:3246875/71‑1 (MQ=255)
aGAACTTACCGGCTCAGGCTGAGCAAAAAGGCGATATGTTGCTGGACGGTTCCCGTCAACTGGCGCGGGaa < 1:3895351/71‑1 (MQ=255)
aGAACTTACCGGCTCAGGCTGAGCAAAAAGGCGATATGTTGCTGGACGGTTCCCGTCAACTGGCGCGGGaa < 1:4606719/71‑1 (MQ=255)
aGAACTTACCGGCTCAGGCTGAGCAAAAAGGCGATATGTTGCTGGACGGTTCCCGTCAACTGGCGCGGGaa < 1:4350211/71‑1 (MQ=255)
aGAACTTACCGGCTCAGGCTGAGCAAAAAGGCGATATGTTGCTGGACGGTTCCCGTCAACTGGCGCGGGaa < 1:2770384/71‑1 (MQ=255)
gAACTTACCGGCTCAGGCTGAGCAAAAAGGCGATATGTTGCTGGACGGTTCCCGTCAACTGGCGCGGGaa < 1:1529171/70‑1 (MQ=255)
gAACTTACCGGCTCAGGCTGAGCAAAAAGGCGATATGTTGCTGGACGGTTCCCGTCAACTGGCGCGGGaa > 1:683216/1‑70 (MQ=255)
gAACTTACCGGCTCAGGCTGAGCAAAAAGGCGATATGTTGCTGGACGGTTCCCGTCAACTGGCGCGGGaa > 1:1599014/1‑70 (MQ=255)
aaCTTACCGGCTCAGGCTGAGCAAAAAGGCGATATGTTGCTGGACGGTTCCCGTCa < 1:2817858/56‑1 (MQ=255)
aaCTTACCGGCTCAGGCTGAGCAAAAAGGCGATATGTTGCTGGACGGTTCCCGTCAACTGGCGCGGGAata < 1:3489684/71‑1 (MQ=255)
aCTTACCGGCTCAGGCTGAGCAAAAAGGCGATATGTTGCTGGACGGTTCCCGTCAACTGGCGCGGGAatat > 1:4642661/1‑71 (MQ=255)
aCTTACCGGCTCAGGCTGAGCAAAAAGGCGATATGTTGCTGGACGGTTCCCGTCAACTGGCGCGGGAatat > 1:76899/1‑71 (MQ=255)
aCTTACCGGCTCAGGCTGAGCAAAAAGGCGATATGTTGCTGGACGGTTCCCGTCAACTGGCGCGGGAatat > 1:2684295/1‑71 (MQ=255)
aCTTACCGGCTCAGGCTGAGCAAAAAGGCGATATGTTGCTGGACGGTTCCCGTCAACTGGCGCGGGAata < 1:1282909/70‑1 (MQ=255)
aCTTACCGGCTCAGGCTGAGCAAAAAGGCGATATGTTGCTGGACGGTTCCCGTCAACTGGCGCGGGAata < 1:2272088/70‑1 (MQ=255)
cTTACCGGCTCAGGCTGAGCAAAAAGGCGATATGTTGCTGGACGGTTCCCGTCAACTGGCGCGGGAATATc > 1:3076134/1‑71 (MQ=255)
cTTACCGGCTCAGGCTGAGCAAAAAGGCGATATGTTGCTGGACGGTTCCCGTCAACTGGCGCGGGAATATc > 1:504714/1‑71 (MQ=255)
cTTACCGGCTCAGGCTGAGCAAAAAGGCGATATGTTGCTGGACGGTTCCCGTCAACTGGCGCGGGAATATc > 1:4011858/1‑71 (MQ=255)
tACCGGCTCAGGCTGAGCAAAAAGGCGATATGTTGCTGGACGGTTCCCGTCAACTGGCGCGGGaa > 1:2351775/1‑65 (MQ=255)
tACCGGCTCAGGCTGAGCAAAAAGGCGATATGTTGCTGGACGGTTCCCGTCAACTGGCGCGGGaa > 1:797620/1‑65 (MQ=255)
aCCGGCTCAGGCTGAGCAAAAAGGCGATATGTTGCTGGACGGTTCCCGTCa < 1:4490525/51‑1 (MQ=255)
ccGGCTCAGGCTGAGCAAAAAGGCGATATGTTGCTGGACGGTTCCCGTCAACTGGCGCGGGAATATCCCg < 1:2535346/70‑1 (MQ=255)
ccGGCTCAGGCTGAGCAAAAAGGCGATATGTTGCTGGACGGTTCCCGTCAACTGGCGCGGGAATATCCCg < 1:2133074/70‑1 (MQ=255)
ccGGCTCAGGCTGAGCAAAAAGGCGATATGTTGCTGGACGGTTCCCGTCAACTGGCGCGGGAATATCCCGa < 1:2181541/71‑1 (MQ=255)
cAGGCTGAGCAAAAAGGCGATATGTTGCTGGACGGTTCCCGTc < 1:4164438/43‑1 (MQ=255)
cAGGCTGAGCAAAAAGGCGATATGTTGCTGGACGGTTCCCGTCAACTGGCGCGGGAATATCCCGAt > 1:3578818/1‑66 (MQ=255)
aGGCTGAGCAAAAAGGCGATATGTTGCTGGACGGTTCCCGTCAACTGGCGCGGGAATATCCCGATCTGGt < 1:3234433/70‑1 (MQ=255)
aGGCTGAGCAAAAAGGCGATATGTTGCTGGACGGTTCCCGTCAACTGGCGCGGGAATATCCCGATCTGGTa < 1:3127330/71‑1 (MQ=255)
ggCTGAGCAAAAAGGCGATATGTTGCTGGACGGTTCCCGTCAACTGGCGCGGGAATATCCCGATCTgg < 1:3987136/68‑1 (MQ=255)
gCTGAGCAAAAAGGCGATATGTTGCTGGACGGTTCCCGTCAACTGGCGCGGGaa < 1:459464/54‑1 (MQ=255)
cTGAGCAAAAAGGCGATATGTTGCTGGACGGTTCCGGTCAACTGGCGCGGGAATATCCCGATCTGGTACAg > 1:4245055/1‑71 (MQ=255)
cTGAGCAAAAAGGCGATATGTTGCTGGACGGTTCCCGTCAACTGGCGCGGGAATATCCCGATCTGGTACAg > 1:582067/1‑71 (MQ=255)
tGAGCAAAAAGGCGATATGTTGCTGGACGGTTCCCGTCAACTGGCGCGGGAATATCCCGATCTGGTACAg > 1:2922427/1‑70 (MQ=255)
tGAGCAAAAAGGCGATATGTTGCTGGACGGTTCCCGTCAACTGGCGCGGGAATATCCCGATCTGGTACAg > 1:873656/1‑70 (MQ=255)
tGAGCAAAAAGGCGATATGTTGCTGGACGGTTCCCGTCAACTGGCGCGGGAATATCCCGATCTGGTACAg > 1:828885/1‑70 (MQ=255)
tGAGCAAAAAGGCGATATGTTGCTGGACGGTTCCCGTCAACTGGCGCGGGAATATCCCGATCTGGTACAg > 1:3240280/1‑70 (MQ=255)
aGCAAAAAGGCGATATGTTGCTGGACGGTTCCCGTCAACTGGCGCGGGAATATCCCGATCTGGTACAgg < 1:2094333/69‑1 (MQ=255)
aGCAAAAAGGCGATATGTTGCTGGACGGTTCCCGTCAACTGGCGCGGGAATATCCCGATCTGGTACAgg < 1:237028/69‑1 (MQ=255)
gCAAAAAGGCGATATGTTGCTGGACGGTTCCCGTCAACTGGCGCGGGAATATCCCGATCTGGTACAGGaa > 1:3935271/1‑70 (MQ=255)
aaaaaGGCGATATGTTGCTGGACGGTTCCCGTCAACTGGCGCGGGAATATCCCGATCTGGTa < 1:371307/62‑1 (MQ=255)
aaGGCGATATGTTGCTGGACGGTTCCCGTCAACTGGCGCGGGAATATCCCGATc > 1:516781/1‑54 (MQ=255)
gCGATATGTTGCTGGACGGTTCCCGTCAACTGGCGCGGGAATATCCCGATCTGGTACAGGAAGCGCGTGGt < 1:633326/71‑1 (MQ=255)
cGATATGTTGCTGGACGGTTCCCGTCAACTGGCGCGGGAATATCCCGATCTGGTACAGGAAGCGCGTGGTa < 1:3047010/71‑1 (MQ=255)
gATATGTTGCTGGACGGTTCCCGTCAACTGGCGCGGGAATATCCCGATCTGGTACAGGAAGCGCGTGGTaa < 1:2063199/71‑1 (MQ=255)
atatGTTGCTGGCCGGTTCCCGGCAACTGGCGCggg < 1:1795130/36‑1 (MQ=25)
atatGTTGCTGGACGGTTCCCGTCAACTGGCGCggg < 1:1247380/36‑1 (MQ=255)
atatGTTGCTGGACGGTTCCCGTCAACTGGCGCggg < 1:3683244/36‑1 (MQ=255)
atatGTTGCTGGACGGTTCCCGTCAACTGGCGCggg < 1:3662442/36‑1 (MQ=255)
tatGTTGCTGGACGGTTCCCGTCAACTGGCGCGGGaa > 1:3673022/1‑37 (MQ=255)
atGTTGCTGGACGGTTCCCGTCAACTGGCGCGGGaa > 1:3068927/1‑36 (MQ=255)
atGTTGCTGGACGGTTCCCGTCAACTGGCGCGGGAATATCCCGATCTGGTACAGGAAGCGCGTGGTAAAgg > 1:4147682/1‑71 (MQ=255)
atGTTGCTGGACGGTTCCCGTCAACTGGCGCGGGAATATCCCGATCTGGTACAGGAAGCGCGTGGTAAAgg > 1:2708643/1‑71 (MQ=255)
tGTTGCTGGACGGTTCCCGTCAACTGGCGCGGGAATATCCCGATCTGGTACAGGAAGCGCGTGGTAAAgg < 1:1666985/70‑1 (MQ=255)
tGTTGCTGGACGGTTCCCGTCAACTGGCGCGGGAATATCCCGATCTGGTACAGGAAGCGCGTGGTAAAgg < 1:1889752/70‑1 (MQ=255)
tGTTGCTGGACGGTTCCCGTCAACTGGCGCGGGAATATCCCGATCTGGTACAGGAAGCGCGTGGTAAAgg < 1:2006040/70‑1 (MQ=255)
ttGCTGGACGGTTCCCGTCAACTGGCGCGGGAATATCCCGATCTTGTACAGGAAGCGCGTGGTAAAGGGAt > 1:983598/1‑71 (MQ=255)
ttGCTGGACGGTTCCCGTCAACTGGCGCGGGAATATCCCGATCTGGTACAGGAAGCGCGTGGTAAAGGGa < 1:1237343/70‑1 (MQ=255)
ttGCTGGACGGTTCCCGTCAACTGGCGCGGGAATATCCCGATCTGGTACAGGAAGCGCGTGGTAAAGGGAt > 1:2159088/1‑71 (MQ=255)
ttGCTGGACGGTTCCCGTCAACTGGCGCGGGAATATCCCGATCTGGTACAGGAAGCGCGTGGTAAAGGGAt > 1:139558/1‑71 (MQ=255)
ttGCTGGACGGTTCCCGTCAACTGGCGCGGGAATATCCCGATCTGGTACAGGAAGCGCGTGGTAAAGGGAt > 1:3873070/1‑71 (MQ=255)
ttGCTGGACGGTTCCCGTCAACTGGCGCGGGAATATCCCGATCTGGTACAGGAAGCGCGTGGTAAAGGGAt > 1:2685128/1‑71 (MQ=255)
gCTGGACGGTTCCCGTCAACTGGCGCGGGAATATCCCGATCTGGTACAGGAAGCGCGTGGTAAAGGGATg < 1:2474493/70‑1 (MQ=255)
cTGGACGGTTCTCGTCAACTGGCGCGGGAATATCCCGATCTGGTACAGGAAGCGCGTGGTAAAGGGATGtt < 1:874690/71‑1 (MQ=255)
cTGGACGGTTCCCGTCAACTGGCGCGGGAATATCCCGATCTGGTACAGGACGCGCGTGGTAAAGGGATGtt < 1:2061291/71‑1 (MQ=255)
cTGGACGGTTCCCGTCAACTGGCGCGGGAATATCCCGATCTGGTACAGGAAGCGTGTGGTAAAGGGATGtt < 1:2046497/71‑1 (MQ=255)
cTGGACGGTTCCCGTCAACTGGCGCGGGAATATCCCGATCTGGTACAGGAAGCGCGTGGTAAAGGGATGtt < 1:55381/71‑1 (MQ=255)
cTGGACGGTTCCCGTCAACTGGCGCGGGAATATCCCGATCTGGTACAGGAAGCGCGTGGTAAAGGGATGtt < 1:3736366/71‑1 (MQ=255)
cTGGACGGTTCCCGTCAACTGGCGCGGGAATATCCCGATCTGGTACAGGAAGCGCGTGGTAAAGGGATGtt < 1:900886/71‑1 (MQ=255)
cTGGACGGTTCCCGTCAACTGGCGCGGGAATATCCCGATCTGGTACAGGAAGCGCGTGGTAAAGGGATGtt < 1:3099436/71‑1 (MQ=255)
cTGGACGGTTCCCGTCAACTGGCGCGGGAATATCCCGATCTGGTACAGGAAGCGCGTGGTAAAGGGATGtt < 1:832013/71‑1 (MQ=255)
cTGGACGGTTCCCGTCAACTGGCGCGGGAATATCCCGATCTGGTACAGGAAGCGCGTGGTAAAGGGATGtt < 1:173461/71‑1 (MQ=255)
cTGGACGGTTCCCGTCAACTGGCGCGGGAATATCCCGATCTGGTACAGGAAGCGCGTGGTAAAGGGATGtt < 1:187165/71‑1 (MQ=255)
cTGGACGGTTCCCGTCAACTGGCGCGGGAATATCCCGATCTGGTACAGGAAGCGCGTGGTAAAGGGATGtt < 1:797090/71‑1 (MQ=255)
cTGGACGGTTCCCGTCAACTGGCGCGGGAATATCCCGATCTGGTACAGGAAGCGCGTGGTAAAGGGATGtt < 1:219453/71‑1 (MQ=255)
gACGGTTCCCGTCAACTGGCGCGGGAATATCCCGATCTGGTACAGGAAGCGCGTGGTAAAGGGATGtt < 1:2240153/68‑1 (MQ=255)
gACGGTTCCCGTCAACTGGCGCGGGAATATCCCGATCTGGTACAGGAAGCGCGTGGTAAAGGGATGtt < 1:2207385/68‑1 (MQ=255)
aCGGTTCCCGTCAACTGGCGCGGGAATATCCCGATCTGGTACAGGAAGCGCGTGGTAAAGGGATGTTGa > 1:4130745/1‑69 (MQ=255)
gTTCCCGTCAACTGGCGCGGGAATATCCCGATCTGGTACAGGAAGCGCGTGGTAAAGGGATGtt < 1:1644662/64‑1 (MQ=255)
|
AATGTGTTGCTGGAGCAGAACTTACCGGCTCAGGCTGAGCAAAAAGGCGATATGTTGCTGGACGGTTTCCGTCAACTGGCGCGGGAATATCCCGATCTGGTACAGGAAGCGCGTGGTAAAGGGATGTTGA > W3110S.gb/3219191‑3219320
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
