Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F8 I0 R1
|
311 |
50.1 |
4085234 |
95.9% |
3917739 |
60.7 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
W3110S.gb |
3,222,206 |
C→T |
8.8% |
N306N (AAC→AAT) |
ebgA → |
cryptic beta‑D‑galactosidase, alpha subunit |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 3,222,206 | 0 | C | T | 8.8%
| 208.7
/ 8.6
| 68 | N306N (AAC→AAT) | ebgA | cryptic beta‑D‑galactosidase, alpha subunit |
| Reads supporting (aligned to +/- strand): ref base C (34/28); new base T (4/2); total (38/30) |
| Fisher's exact test for biased strand distribution p-value = 6.87e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01 |
CAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAACGGCAACGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATC > W3110S.gb/3222138‑3222275
|
cagcaATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAACGGCAACGt < 1:953953/71‑1 (MQ=255)
cagcaATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAACGGCAACGt < 1:2680331/71‑1 (MQ=255)
cagcaATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAACGGCAACGt < 1:2052336/71‑1 (MQ=255)
agcaATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAACGGCAACGt < 1:1494918/70‑1 (MQ=255)
aaTGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACCCTGAAAGACGCCAACGGCAACGTTCTg > 1:4080999/1‑71 (MQ=255)
aTGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAACGGCAACGTTCTgg < 1:2234930/71‑1 (MQ=255)
aTGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAACGACAACGTTCTgg < 1:2876455/71‑1 (MQ=255)
gTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAACGGCAATGTTCttg > 1:206929/1‑66 (MQ=255)
gTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAACGGCAATGTTCTgg > 1:1311610/1‑68 (MQ=255)
gTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAACGGCAATGTTCTgg > 1:232290/1‑68 (MQ=255)
gTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAACGGCAACGTTCTGGAAg > 1:1180702/1‑71 (MQ=255)
agAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAACGGCAACGtt > 1:936254/1‑58 (MQ=255)
aaTCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAACGGCAACGTTCTGGAAgt < 1:1420708/64‑1 (MQ=255)
aTCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAACGGCAACGTTCTGGAAGTGGTGCcac < 1:2637517/71‑1 (MQ=255)
aTCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAACGGCAACGTTCTGGAAGT‑GTGCcac > 1:1190634/1‑70 (MQ=255)
tCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAACGGCAACGTTCTGGAAGTGGTGCcac > 1:2858473/1‑70 (MQ=255)
ccccTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAACGGCAACGTTCTGGaa > 1:2593799/1‑59 (MQ=255)
cccTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAACGGCAACGtt > 1:3124564/1‑52 (MQ=255)
cccTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAACGGCAACGTTCTGGaa > 1:1758822/1‑58 (MQ=255)
cccTTATCTTTACCATCAGGTCATGACGCTGAAAGACGCCAACGGCAACGTTCTGGaa > 1:3474959/1‑58 (MQ=255)
ttATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAACGGCAACGTTCTGGAAGTGGTGCCACAAcgcg > 1:1569892/1‑71 (MQ=255)
tATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAACGGCATCGTTCTGGAATTGGTGCCACAAcgc < 1:144542/69‑1 (MQ=255)
tATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAACGGCAACGTTCTGGAAGTGGTGCCACAACGCGt > 1:3454655/1‑71 (MQ=255)
tATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAACGGCAACGTTCTGGAAGTGGTGCCACAACGCGt > 1:289194/1‑71 (MQ=255)
aTCTTTACCATCTGGTCATGACGCTGAAAGACGCCAACGGCAATGTTCTGGAAGTGGTGCCACAACGCGtt > 1:1753755/1‑71 (MQ=255)
aTCTTTACCATCTGGTCATGACGCTGAAAGACGCCAACGGCAACGTTCTGGaa > 1:1749313/1‑53 (MQ=255)
aTCTTTACCATCTGGTCATGACGCTGAAAGACGCCAACGGCAACGTTCTGGAAgtgg > 1:2325323/1‑57 (MQ=255)
aTCTTTACCATCTGGTCATGACGCTGAAAGACGCCAACGGCAACGTTCTGGAAGTGGTGCCACAACGCGtt > 1:1342195/1‑71 (MQ=255)
aTCTTTACCATCTGGTCATGACGCTGAAAGACGCCAACGGCAACGTTCTGGAAGTGGTGCCACAACGCGtt > 1:2573641/1‑71 (MQ=255)
tCTTTACCATCTGGTCATGACGCTGAAAGACGCCAACGGCAACGTTCTGGAAGTGGTGCCACAACGCGTTg > 1:810656/1‑71 (MQ=255)
cTTTACCATCTGGTCATGACGCTGAAAGACGCCAACGGCAACGTTCTGGAAGTGGTGCcac < 1:139027/61‑1 (MQ=255)
tttACCATCTGGTCATGACGCTGAAAGACGCCAACGGCAACGTTCTGGAAGTGGTGCCACAACGCGTTGGc > 1:2721121/1‑71 (MQ=255)
tttACCATCTGGTCATGACGCTGAAAGACGCCAACGGCAACGTTCTGGAAGTGGTGCCACAACGCGTTGGc > 1:1178421/1‑71 (MQ=255)
tttACCATCTGGTCATGACGCTGAAAGACGCCAACGGCAACGTTCTGCAAGTGGTGCCACAACGCGTTGGc > 1:652854/1‑71 (MQ=255)
aTCTGGTCATGACGCTGAAAGACGCCAACGGCAACGTTCTGGAAGTTGTGCCACAACGCGTTGGCTTCCGt < 1:744023/71‑1 (MQ=255)
aTCTGGTCATGACGCTGAAAGACGCCAACGGCAACGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGt < 1:1810736/71‑1 (MQ=255)
aTCTGGTCATGACGCTGAAAGACGCCAACGGCAACGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGt < 1:927378/71‑1 (MQ=255)
aTCTGGTCATGACGCTGAAAGACGCCAACGGCAACGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGt < 1:2264431/71‑1 (MQ=255)
tCTGGTCATGACGCTGAAAGACGCCAACGGCAACGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGt > 1:2258338/1‑70 (MQ=255)
gTCATGACGCTGAAAGACGCCAACGGCAATGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGa < 1:3305433/68‑1 (MQ=255)
gTCATGACGCTGAAAGACGCCAACGGCAACGTTCTGGAAGTGGTGCCACAACTCGTTGGCTTCCGTg < 1:3385898/67‑1 (MQ=255)
tCATGACGCTGAAAGACGCCAACGGCAACGTTCTGGAAgt < 1:1817340/40‑1 (MQ=255)
tCATGACGCTGAAAGACGCCAACGGCAACGTTCTGGAAgt < 1:763325/40‑1 (MQ=255)
cATGACGCTGAAAGACGCCAACGGCAACGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATc > 1:1274095/1‑70 (MQ=255)
aTGACGCTGAAAGACGCTAACGGCAACGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATCa < 1:2549169/70‑1 (MQ=255)
aTGACGCTGAAAGACGCCAACGGCAACGTTCTGGAAGTGGTGCcac < 1:2763314/46‑1 (MQ=255)
aTGACGCTGAAAGACGCCAACGGCAACGTTCTGGAAGTGGTGCcac < 1:942999/46‑1 (MQ=255)
aTGACGCTGAAAGACGCCAACGGCAACGTTCTGGAAGTGGTGCcac < 1:938134/46‑1 (MQ=255)
tGACGCTGAAAGACGCCAACGGCAACGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATCa < 1:4060533/69‑1 (MQ=255)
gACGCTGAAAGACGCCAACGGCAACGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATCAAAg > 1:4082907/1‑71 (MQ=255)
gACGCTGAAAGACGCCAACGGCAACGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATCAAAg > 1:3156714/1‑71 (MQ=255)
gACGCTGAAAGACGCCAACGGCAACGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATCAAAg > 1:50206/1‑71 (MQ=255)
aCGCTGAAAGACGCCAACGGCAACGTTCTGGAAGTGGTGCCACaa > 1:431877/1‑45 (MQ=255)
aCGCTGAAAGACGCCAACGGCAACGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATCaaa > 1:3683716/1‑69 (MQ=255)
aCGCTGAAAGACGCCAACGGCAACGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATCaaa > 1:3508892/1‑69 (MQ=255)
aCGCTGAAAGACGCCAACGGCAACGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATCaaa > 1:2089465/1‑69 (MQ=255)
aaGACGCCAACGGCAACGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGAc > 1:3509061/1‑71 (MQ=255)
aaGACGCCAACGGCAACGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGAc > 1:3197849/1‑71 (MQ=255)
ccAACGGCAATGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGa < 1:3269500/49‑1 (MQ=255)
ccAACGGCAACGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATCa < 1:3283618/54‑1 (MQ=255)
ccAACGGCAACGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTg < 1:3575399/71‑1 (MQ=255)
ccAACGGCAACGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTg < 1:1833628/71‑1 (MQ=255)
ccAACGGCAACGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTg < 1:2385274/71‑1 (MQ=255)
aaCGGCAACGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGt > 1:3061718/1‑70 (MQ=255)
aCGGCAACGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTc < 1:181728/71‑1 (MQ=255)
ggCAACGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGt > 1:1211826/1‑63 (MQ=255)
aaCGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGtctggtctgg > 1:229196/1‑69 (MQ=255)
aCGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATc < 1:3181671/71‑1 (MQ=255)
|
CAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAACGGCAACGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATC > W3110S.gb/3222138‑3222275
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
