Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F28 I2 R1
|
330 |
0.0 |
2544031 |
91.9% |
2337964 |
62.1 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
3,222,206 |
C→T |
N306N (AAC→AAT) |
ebgA → |
cryptic beta‑D‑galactosidase, alpha subunit |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 3,222,206 | 0 | C | T | 100.0%
| 11.8
/ NA
| 5 | N306N (AAC→AAT) | ebgA | cryptic beta‑D‑galactosidase, alpha subunit |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (5/0); total (5/0) |
ACGCTGAAAGACGCCAACGGCAACGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGATA > W3110S.gb/3222183‑3222246
|
aCGCTGAAAGACGCCAACGGCAATGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGata > 1:1410774/1‑64 (MQ=255)
aCGCTGAAAGACGCCAACGGCAATGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGata > 1:1634701/1‑64 (MQ=255)
aCGCTGAAAGACGCCAACGGCAATGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGata > 1:1799596/1‑64 (MQ=255)
aCGCTGAAAGACGCCAACGGCAATGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGata > 1:722245/1‑64 (MQ=255)
aCGCTGAAAGACGCCAACGGCAATGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGata > 1:982693/1‑64 (MQ=255)
|
ACGCTGAAAGACGCCAACGGCAACGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGATA > W3110S.gb/3222183‑3222246
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
