Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F28 I1 R2
|
328 |
63.0 |
4687567 |
98.0% |
4593815 |
65.2 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
3,634,018 |
T→C |
V46A (GTA→GCA) |
yigF → |
conserved inner membrane protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 3,634,018 | 0 | T | C | 100.0%
| 266.4
/ NA
| 78 | V46A (GTA→GCA) | yigF | conserved inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (29/49); total (29/49) |
TCAACATGGTTTTCCTGGATTCTGGGGGGCAACACCAGAATATAAAGCAGCATTTAAAGCATTAAAAGTAAGACAACGCTTAACTATTCAAATGAATTTCATTGCTTTCTTCTGTTCCTGGATATATTTATTTG > W3110S.gb/3633950‑3634083
|
tCAACATGGTTTTCCTGGATTCTGGGGGGCAACACCAGAATATAAAGCAGCATTTAAAGCATTAAAAGCaa < 1:4645651/71‑1 (MQ=255)
aCATGGTTTTCCTGGATTCTGGGGGGCAACACCAGAATATAAAGCAGCATTTAAAGCATTAAAAGCAAGAc < 1:2039924/71‑1 (MQ=255)
aCATGGTTTTCCTGGATTCTGGGGGGCAACACCAGAATATAAAGCAGCATTTAAAGCATTAAAAGCAAGAc < 1:1935742/71‑1 (MQ=255)
aCATGGTTTTCCTGGATTCTGGGGGGCAACACCAGAATATAAAGCAGCATTTAAAGCATTAAAAGCAAGAc < 1:3774461/71‑1 (MQ=255)
aCATGGTTTTCCTGGATTCTGGGGGGCAACACCAGAATATAAAGCAGCATTTAAAGCATTAAAAGCAAGAc < 1:625222/71‑1 (MQ=255)
ttCCTGGATTCTGGGGGGCAACACCAGAATATAAAGCAGCATTTAAAGCATTAAAAGCAAGACAACGCtt > 1:1102292/1‑70 (MQ=255)
ttCCTGGATTCTGGGGGGCAACACCAGAATATAAAGCAGCATTTAAAGCATTAAAAGCAAGACAACGCtt < 1:3076721/70‑1 (MQ=255)
ttCCTGGATTCTGGGGGGCAACACCAGAATATAAAGCAGCATTTAAAGCATTAAAAGCAAGACAACGCtt < 1:1561183/70‑1 (MQ=255)
ttCCTGGATTCTGGGGGGCAACACCAGAATATAAAGCAGCATTTAAAGCATTAAAAGCAAGACAACGCtt < 1:429986/70‑1 (MQ=255)
tCCTGGATTCTGGGGGGCAACACCAGAATATAAAGCAGCATTTAAAGCATTAAAAGCAAGACAACGCTTa < 1:2563417/70‑1 (MQ=255)
tCCTGGATTCTGGGGGGCAACACCAGAATATAAAGCAGCATTTAAAGCATTAAAAGCAAGACAACGCTTa < 1:2795363/70‑1 (MQ=255)
ttCTGGGGGGCAACACCAGAATATAAAGCAGCATTTAAAGCATTAAAAGCAAGACAACGCTTAACTATTCa < 1:794783/71‑1 (MQ=255)
ttCTGGGGGGCAACACCAGAATATAAAGCAGCATTTAAAGCATTAAAAGCAAGACAACGCTTAACTATTCa < 1:1118822/71‑1 (MQ=255)
cTGGGGGGCAACACCAGAATATAAAGCAGCATTTAAAGCATTAAAAGCAAGACAACGCTTAACTa > 1:3420490/1‑65 (MQ=255)
gggggCAACACCAGAATATAAAGCAGCATTTAAAGCATTAAAAGCAAGACAACGCTTAACTATTCAAATg < 1:3225294/70‑1 (MQ=255)
gggggCAACACCAGAATATAAAGCAGCATTTAAAGCATTAAAAGCAAGACAACGCTTAACTATTCAAATg < 1:1759737/70‑1 (MQ=255)
gggggCAACACCAGAATATAAAGCAGCATTTAAAGCATTAAAAGCAAGACAACGCTTAACTATTCAAATg < 1:2256575/70‑1 (MQ=255)
gggggCAACACCAGAATATAAAGCAGCATTTAAAGCATTAAAAGCAAGACAACGCTTAACTATTCAAATg < 1:4665158/70‑1 (MQ=255)
gggggCAACACCAGAATATAAAGCAGCATTTAAAGCATTAAAAGCAAGACAACGCTTAACTATTCAAATg < 1:2455603/70‑1 (MQ=255)
gCAACACCAGAATATAAAGCAGCATTTAAAGCATTAAAAGCAAGACAACGCTTAACTATTCAAATg < 1:678295/66‑1 (MQ=255)
gCAACACCAGAATATAAAGCAGCATTTAAAGCATTAAAAGCAAGACAACGCTTAACTATTCAAATGAAttt < 1:124189/71‑1 (MQ=255)
gCAACACCAGAATATAAAGCAGCATTTAAAGCATTAAAAGCAAGACAACGCTTAACTATTCAAATGAAttt < 1:3352719/71‑1 (MQ=255)
cAACACCAGAATATAAAGCAGCATTTAAAGCATTAAAAGCAAGACAACGCTTAACTATTCAAATGTATTTc < 1:4621043/71‑1 (MQ=255)
acacCAGAATATAAAGCAGCATTTAAAGCATTAAAAGCAAGAc < 1:2835274/43‑1 (MQ=255)
acacCAGAATATAAAGCAGCATTTAAAGCATTAAAAGCAAGACAACGCtt < 1:2897920/50‑1 (MQ=255)
cacCAGAATATAAAGCAGCATTTAAAGCATTAAAAGCAAGACAACGCtt < 1:225526/49‑1 (MQ=255)
cacCAGAATATAAAGCAGCATTTAAAGCATTAAAAGCAAGACAACGCtt < 1:615888/49‑1 (MQ=255)
cacCAGAATATAAAGCAGCATTTAAAGCATTAAAAGCAAGACAACGCtt < 1:1667775/49‑1 (MQ=255)
cacCAGAATATAAAGCAGCATTTAAAGCATTAAAAGCAAGACAACGCtt < 1:1613805/49‑1 (MQ=255)
acCAGAATATAAAGCAGCATTTAAAGCATTAAAAGCAAGACAACGCTTAACTATTCAAATGAATTTCATTg > 1:92069/1‑71 (MQ=255)
acCAGAATATAAAGCAGCATTTAAAGCATTAAAAGCAAGACAACGCTTAACTATTCAAATGAATTTCATTg > 1:3025607/1‑71 (MQ=255)
ccAGAATATAAAGCAGCATTTAAAGCATTAAAAGCAAGACAACGCTTAACTTTTCAAATGAATTTCATTGc < 1:35990/71‑1 (MQ=255)
ccAGAATATAAAGCAGCATTTAAAGCATTAAAAGCAAGACAACGCTTAACTATTCAAATGAATTTCATTGc > 1:1707753/1‑71 (MQ=255)
aGAATATAAAGCAGCATTTAAAGCATTAAAAGCAAGACAACGCTTAACTATTCAAATGAATTTCATTGCtt > 1:2211476/1‑71 (MQ=255)
atatAAAGCAGCATTTAAAGCATTAAAAGCAAGACAACGCTTAACTATTCAAATGAATTTCATTGCTttct > 1:840479/1‑71 (MQ=255)
atatAAAGCAGCATTTAAAGCATTAAAAGCAAGACAACGCTTAACTATTCAAATGAATTTCATTGCTttct > 1:699389/1‑71 (MQ=255)
tataAAGCAGCATTTAAAGCATTAAAAGCAAGACAACGCTTAGCTATTCAAATGAATTTCATTGCTttctt > 1:4239292/1‑71 (MQ=255)
ataAAGCAGCATTTAAAGCATTAAAAGCAAGACAACGCTTAACTATTCAAATGAATTTCATTGCTttctt < 1:4111303/70‑1 (MQ=255)
taAAGCAGCATTTAAAGCATTAAAAGCAAGACAACGCTTAACTATTCAAATGAATTTCAtt > 1:4224003/1‑61 (MQ=255)
taAAGCAGCATTTAAAGCATTAAAAGCAAGACAACGCTTAACTATTCAAATGAATTTCATTGCTTtcttct > 1:897052/1‑71 (MQ=255)
taAAGCAGCATTTAAAGCATTAAAAGCAAGACAACGCTTAACTATTCAAATGAATTTCATTGCTTtcttct > 1:696977/1‑71 (MQ=255)
aaGCAGCATTTAAAGCATTAAAAGCAAGACAACGCTTAACTATTCAAATGAATTTCATTGCTTTCTTCTGt > 1:1130207/1‑71 (MQ=255)
aaGCAGCATTTAAAGCATTAAAAGCAAGACAACGCTTAACTATTCAAATGAATTTCATTGCTTTCTTCTGt < 1:780272/71‑1 (MQ=255)
agcagcATTTAAAGCATTAAAAGCAAGACAACGCtt < 1:625975/36‑1 (MQ=255)
agcagcATTTAAAGCATTAAAAGCAAGACAACGCTTAACTATTCAAATGAATTTCATTGCTTTCTTCTGt < 1:1479288/70‑1 (MQ=255)
agcagcATTTAAAGCATTAAAAGCAAGACAACGCTTAACTATTCAAATGAATTTCATTGCTTTCTTCTGt < 1:3914722/70‑1 (MQ=255)
agcagcATTTAAAGCATTAAAAGCAAGACAACGCTTAACTATTCAAATGAATTTCATTGCTTTCTTCTGt < 1:3970418/70‑1 (MQ=255)
aTTTAAAGCATTAAAAGCAAGACAACGCTTAACTATTCAAATGAATTTCATTGCTTTCTTCTGTTCCTGGa < 1:4059673/71‑1 (MQ=255)
tttAAAGCATTAAAAGCAAGACAACGCTTAACTATTCAAATGAATTTCATTGCTTTCTTCTGt < 1:548926/63‑1 (MQ=255)
tttAAAGCATTAAAAGCAAGACAACGCTTAACTATTCAAATGAATTTCATTGCTTTCTTCTGt < 1:1825760/63‑1 (MQ=255)
tAAAGCATTAAACGCAAGACACCGCTTAACTATTCAAATGAATTTCATTGCt > 1:815092/1‑52 (MQ=37)
tAAAGCATTAAAAGCAAGACAACGCTTAACTATTCAAATGAATTTCAtt > 1:1596682/1‑49 (MQ=255)
tAAAGCATTAAAAGCAAGACAACGCTTAACTATTCAAATGAATTTCATTGCTTtcttct > 1:284416/1‑59 (MQ=255)
tAAAGCATTAAAAGCAAGACAACGCTTAACTATTCAAATGAATTTCATTGCTTTCTTCTGTTCCTGGata > 1:1708758/1‑70 (MQ=255)
tAAAGCATTAAAAGCAAGACAACGCTTAACTATTCAAATGAATTTCATTGCTTTCTTCTGTTCCTGGata > 1:1952202/1‑70 (MQ=255)
tAAAGCATTAAAAGCAAGACAACGCTTAACTATTCAAATGAATTTCATTGCTTTCTTCTGTTCCTGGata > 1:4169427/1‑70 (MQ=255)
tAAAGCATTAAAAGCAAGACAACGCTTAACTATTCAAATGAATTTCATTGCTTTCTTCTGTTCCTGGata > 1:3013413/1‑70 (MQ=255)
tAAAGCATTAAAAGCAAGACAACGCTTAACTATTCAAATGAATTTCATTGCTTTCTTCTGTTCCTGGata > 1:379255/1‑70 (MQ=255)
tAAAGCATTAAAAGCAAGACAACGCTTAACTATTCAAATGAATTTCATTGCTTTCTTCTGTTCCTGGata > 1:3127909/1‑70 (MQ=255)
tAAAGCATTAAAACCAAGACAACGCTTAACTATTCAAATGAATTTCAATGCTTTCTACTGTTCCt > 1:3558088/1‑65 (MQ=255)
aaaGCATTAAAGGCAAGACAACGCTTAACTATTCAAATGAATTTCATTGCTTTCTTCTGTTCCTGGatata > 1:1800831/1‑71 (MQ=255)
aaaGCATTAAAAGCAAGACAACGCTTAACTATTCAAATGAATTTCATTGCTTTCTTCTGTTCCTGGatat < 1:663978/70‑1 (MQ=255)
cATTAAAAGCAAGACAACGCTTAACTATTCAAATGAATTTCATTGCTTTCTTCTGTTCCTGGATAtattta > 1:3358953/1‑71 (MQ=255)
ttAAAAGCAAGACAACTCTTAACTATTCAAATGAATTTCATTGCTTTCTTCTGTTCCTGGATAtattta < 1:2368863/69‑1 (MQ=255)
ttAAAAGCAAGACAACTCTTAACTATTCAAATGAATTTCATTGCTTTCTTCTGTTCCTGGATAtattta < 1:2363580/69‑1 (MQ=255)
ttAAAAGCAAGACAACGCTTAACTATTCAAATGAATTTCATTGCTTTCTTCTGt < 1:2340053/54‑1 (MQ=255)
aaaaGCAAGACAACGCTTAACTATTCAAATGAATTTCa > 1:3476330/1‑38 (MQ=255)
aaaaGCAAGACAACGCTTAACTATTCAAATGAATTTCATTGCTttctt > 1:2951621/1‑48 (MQ=255)
aaaaGCAAGACAACGCTTAACTATTCAAATGAATTTCATTGCTTTCTTCTGTTCCTGGATATATTTATTTg > 1:55223/1‑71 (MQ=255)
aaaaGCAAGACAACGCTTAACTATTCAAATGAATTTCATTGCTTTCTTCTGTTCCTGGATATATTTATTTg > 1:2799910/1‑71 (MQ=255)
aaaGCAAGACAACGCTTAACTATTCAAATGAATTTCATTGCTTTCTTCTGTTCCTGGATATATTTATTTg < 1:3298332/70‑1 (MQ=255)
aaaGCAAGACAACGCTTAACTATTCAAATGAATTTCATTGCTTTCTTCTGTTCCTGGATATATTTATTTg < 1:2368277/70‑1 (MQ=255)
aaaGCAAGACAACGCTTAACTATTCAAATGAATTTCATTGCTTTCTTCTGTTCCTGGATATATTTATTTg < 1:3518432/70‑1 (MQ=255)
aaaGCAAGACAACGCTTAACTATTCAAATGAATTTCATTGCTTTCTTCTGTTCCTGGATATATTTATTTg < 1:541957/70‑1 (MQ=255)
aaaGCAAGACAACGCTTAACTATTCAAATGAATTTCATTGCTTTCTTCTGTTCCTGGATATATTTATTTg < 1:2171433/70‑1 (MQ=255)
aaaGCAAGACAACGCTTAACTATTCAAATGAATTTCATTGCTTTCTTCTGTTCCTGGATATATTTATTTg < 1:891246/70‑1 (MQ=255)
aaaGCAAGACAACGCTTAACTATTCAAATGAATTTCATTGCTTTCTTCTGTTCCTGGATATATTTATTTg < 1:2969043/70‑1 (MQ=255)
aaaGCAAGACAACGCTTAACTATTCAAATGAATTTCATTGCTTTCTTCTGTTCCTGGATATATTTATTTg < 1:3897962/70‑1 (MQ=255)
|
TCAACATGGTTTTCCTGGATTCTGGGGGGCAACACCAGAATATAAAGCAGCATTTAAAGCATTAAAAGTAAGACAACGCTTAACTATTCAAATGAATTTCATTGCTTTCTTCTGTTCCTGGATATATTTATTTG > W3110S.gb/3633950‑3634083
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
