Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F3 I0 R2
|
232 |
76.3 |
4135488 |
90.9% |
3759158 |
60.7 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
2,144,345 |
G→A |
31.0% |
D206N (GAT→AAT) |
argG → |
argininosuccinate synthetase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 2,144,345 | 0 | G | A | 31.0%
| 122.3
/ 74.5
| 87 | D206N (GAT→AAT) | argG | argininosuccinate synthetase |
| Reads supporting (aligned to +/- strand): ref base G (33/27); new base A (11/16); total (44/43) |
| Fisher's exact test for biased strand distribution p-value = 2.52e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.47e-01 |
CAAAATGTCTGTCGAAAAAGCCTACTCCACAGACTCCAACATGCTTGGTGCAACGCATGAAGCGAAGGATCTGGAATACCTCAACTCCAGCGTCAAAATCGTCAACCCGATTATGGGCGTGAAATTCTGGGATGA > minE/2144278‑2144412
|
cAAAATGTCTGTCGAAAAAGCCTACTCCACAGACTCCAACATGCTTGGTGCAACGCATGAAGCGAAGGATc > 1:750543/1‑71 (MQ=255)
cAAAATGTCTGTCGAAAAAGCCTACTCCACAGACTCCAACATGCTTGGTGCAACGCATGAAGCGAAGAATc < 1:2658746/71‑1 (MQ=255)
aaaTGTCTGTCGAAAAAGCCTACTCCACAGACTCCAACATGCTTGGTGCAACGCATGAAGCGAAGAATCTg < 1:1192767/71‑1 (MQ=255)
aaTGTCTGTCGAAAAAGCCTACTCCACAGACTCCAACATGCTTGGTGCAACGCATGAAGCGAAGGATCTgg < 1:1580226/71‑1 (MQ=255)
aaTGTCTGTCGAAAAAGCCTACTCCACAGACTCCAACATGCTTGGTGCAACGCATGAAGCGAAGGATCTgg < 1:1105003/71‑1 (MQ=255)
aaTGTCTGTCGAAAAAGCCTACTCCACAGACTCCAACATGCTTGGTGCAACGCATGAAGCGAAGGATCTgg < 1:2175003/71‑1 (MQ=255)
aaTGTCTGTCGAAAAAGCCTACTCCACAGACTCCAACATGCTTGGTGCAACGCATGAAGCGAAGGATCTgg < 1:2492069/71‑1 (MQ=255)
aaTGTCTGTCGAAAAAGCCTACTCCACAGACTCCAACATGCTTGGTGCAACGCATGAAGCGAAGGATCTgg < 1:1243837/71‑1 (MQ=255)
aTGTCTGTCGAAAAAGCCTACTCCACAGACTCCAACATGCTTGGTGCAACGCATGAAGCGAAGGATCTgg > 1:3011755/1‑70 (MQ=255)
aTGTCTGTCGAAAAAGCCTACTCCACAGACTCCAACATGCTTGGTGCAACGCATGAAGCGAAGGATCTgg > 1:3580168/1‑70 (MQ=255)
aTGTCTGTCGAAAAAGCCTACTCCACAGACTCCAACATGCTTGGTGCAACGCATGAAGCGAAGGATCTg > 1:4010377/1‑69 (MQ=255)
gAAAAAGCCTACTCCACAGACTCCAACATGCTTGGTGCAACGCATGAAGCGAAGGATCTGGAATACCTCaa > 1:1668073/1‑71 (MQ=255)
gAAAAAGCCTACTCCACAGACTCCAACATGCTTGGTGCAACGCATGAAGCGAAGGATCTGGAATACCTCaa > 1:1111247/1‑71 (MQ=255)
gAAAAAGCCTACTCCACAGACTCCAACATGCTTGGTGCAACGCATGAAGCGAAGGATCTGGAATACCTCaa > 1:468186/1‑71 (MQ=255)
gAAAAAGCCTACTCCACAGACTCCAACATGCTTGGTGCAACGCATGAAGCGAAGGATCTGGAATACCTCaa > 1:3067744/1‑71 (MQ=255)
aaGCCTACTCCACAGACTCCAACATGCTTGGTGCAACGCATGAAGCGAAGGATCTGGAATACCTCAACTcc < 1:2530824/71‑1 (MQ=255)
tACTCCACAGACTCCAACATGCTTGGTGCAACGCATGAAGCGAAgg < 1:2175890/46‑1 (MQ=255)
tACTCCACAGACTCCAACATGCTTGGTGCAACGCATGAAGCGAAgg < 1:1596347/46‑1 (MQ=255)
tCCACAGACTCCAACATGCTTGGTGCAACGCATGAAGCGAAGGATCTgg < 1:1067677/49‑1 (MQ=255)
tCCACAGACTCCAACATGCTTGGTGCAACGCATGAAGCGAAGGATCTgg < 1:582113/49‑1 (MQ=255)
tCCACAGACTCCAACATGCTTGGTGCAACGCATGAAGCGAAGGATCTgg < 1:990924/49‑1 (MQ=255)
tCCACAGACTCCAACATGCTTGGTGCAACGCATGAAGCGAAGAATCTGGAATACCTCAACTCCAGCGTCa > 1:2868906/1‑70 (MQ=255)
cacaGACTCCAACATGCTTGGTGCAACGCATGAAGCGAAGGa > 1:564447/1‑42 (MQ=255)
cacaGACTCCAACATGCTTGGTGCAACGCATGAAGCGAAGGAt > 1:460427/1‑43 (MQ=255)
cacaGACTCCAACATGCTTGGTGCAACGCATGAAGCGAAGGAt > 1:3586204/1‑43 (MQ=255)
cacaGACTCCAACATGCTTGGTGCAACGCATGAAGCGAAGGAt > 1:1813361/1‑43 (MQ=255)
cacaGACTCCAACATGCTTGGTGCAACGCATGAAGCGAAGGATc > 1:4092000/1‑44 (MQ=255)
cacaGACTCCAACATGCTTGGTGCAACGCATGAAGCGAAGGATCTg > 1:1185145/1‑46 (MQ=255)
cacaGACTCCAACATGCTTGGTGCAACGCATGAAGCGAAGGATCTGGAATACCTCAACTCCAGCGTCaaaa > 1:3014940/1‑71 (MQ=255)
cacaGACTCCAACATGCTTGGTGCAACGCATGAAGCGAAGGATCTGGAATACCTCAACTCCAGCGTCaaaa < 1:3718338/71‑1 (MQ=255)
cacaGACTCCAACATGCTTGGTGCAACGCATGAAGCGAAGGATCTGGAATACCTCAACTCCAGCGTCaaaa > 1:2221489/1‑71 (MQ=255)
cacaGACTCCAACATGCTTGGTGCAACGCATGAAGCGAAGGATCTGGAATACCTCAACTCCAGCGTCaaaa > 1:1309890/1‑71 (MQ=255)
cacaGACTCCAACATGCTTGGTGCAACGCATGAAGCGAAGGATCTGGAATACCTCAACTCCAGCGTCaaaa > 1:2478378/1‑71 (MQ=255)
cacaGACTCCAACATGCTTGGTGCAACGCATGAAGCGAAGGATCTGGAATACCTCAACTCCAGCGTCaaa > 1:1652193/1‑70 (MQ=255)
cacaGACTCCAACATGCTTGGTGCAACGCATGAAGCGAAGGATCTGGAATACCTCAACTCCAGCGTCaaa < 1:1386910/70‑1 (MQ=255)
cacaGACTCCAACATGCTTGGTGCAACGCATGAAGAGAAGGAt > 1:963293/1‑43 (MQ=255)
caGACTCCAACATGCTTGGTGCAACGCATGAAGCGAAGAATCTGGAATACCTCAACTCCAGCGTCAAAATc > 1:2741175/1‑71 (MQ=255)
aCTCCAACATGCTTGGTGCAACGCATGAAGCGAAGGATCTGGAATGCCTCAACTCCAGCGTCAAAATCGTc < 1:337590/71‑1 (MQ=255)
aCTCCAACATGCTTGGTGCAACGCATGAAGCGAAGGATCTGGAATACCTCAACTCCAGCGTCAAAATCGTc < 1:1493088/71‑1 (MQ=255)
aCTCCAACATGCTTGGTGCAACGCATGAAGCGAAGGATCTGGAATACCTCAACTCCAGCGTCAAAATCGTc < 1:3316588/71‑1 (MQ=255)
cTCCAACATGCTTGGTGCAACGCATGAAGCGAAGGATCTGGAATACCTCAACTCCAGCGTCAAAATCGTc < 1:2598872/70‑1 (MQ=255)
cATGCTTGGTGCAACGCATGAAGCGAAGAATCTgg < 1:3447966/35‑1 (MQ=255)
cATGCTTGGTGCAACGCATGAAGCGAAGAATCTgg < 1:993147/35‑1 (MQ=255)
cATGCTTGGTGCAACGCATGAAGCGAAGAATCTgg < 1:563971/35‑1 (MQ=255)
cATGCTTGGTGCAACGCATGAAGCGAAGAATCTgg < 1:355079/35‑1 (MQ=255)
cATGCTTGGTGCAACGCATGAAGCGAAGAATCTgg < 1:3297660/35‑1 (MQ=255)
cATGCTTGGTGCAACGCATGAAGCGAAGAATCTgg < 1:3050354/35‑1 (MQ=255)
cATGCTTGGTGCAACGCATGAAGCGAAGAATCTGGAATACCTCAACTCCAGCGTCAAAATCGTCaa > 1:2793215/1‑66 (MQ=255)
tGCTTGGTGCAACGCATGAAGCGAAGGATCTGGAATACCTCAACTCCAGCGTCAAAATCGTCAACCCGAtt < 1:564233/71‑1 (MQ=255)
cTTGGTGCAACGCATGAAGCGAAGGATCTGGAATACCTCAACTCCAGCGTCAAAATCGTCAACCCGATTa < 1:3579563/70‑1 (MQ=255)
gTGCAACGCATGAAGCGAAGAATCTGGAATACCTCAACTCCAGCGTCAAAAt > 1:3169697/1‑52 (MQ=255)
gTGCAACGCATGAAGCGAAGAATCTGGAATACCTCAACTCCAGCGTCAAAAt > 1:1585351/1‑52 (MQ=255)
gTGCAACGCATGAAGCGAAGAATCTGGAATACCTCAACTCCAGCGTCAAAAt > 1:380636/1‑52 (MQ=255)
aaCGCATGAAGCGAAGGATCTGGAATACCTCAACTCCAGCGTCAAAATCGTCAACCCGATTATGGGCGTGa < 1:1446405/71‑1 (MQ=255)
aaCGCATGAAGCGAAGGATCTGGAATACCTCAACTCCAGCGGCAAAATCGTCAACCCGATTATGGGCGTGa < 1:3515623/71‑1 (MQ=255)
gCATGAAGCGAAGGATCTGGAATACCTCAACTCCAGCGTCAAAATCGTc > 1:247580/1‑49 (MQ=255)
aTGAAGCGAAGGATCTGGAATACCTCAACTCCAGCGTCAAAATCGTCAACCCGATTATGGGCGTGAAAtt > 1:236937/1‑70 (MQ=255)
aTGAAGCGAAGGATCTGGAATACCTCAACTCCAGCGTCAAAATCGTCAACCCGATTATGGGCGTGAAATTc > 1:1768207/1‑71 (MQ=255)
aTGAAGCGAAGGATCTGGAATACCTCAACTCCAGCGTCAAAATCGTCAACCCGATTATGGGCGTGAAATTc > 1:712348/1‑71 (MQ=255)
aTGAAGCGAAGGATCTGGAATACCTCAACTCCAGCGTCAAAATCGTCAACCCGATTATGGGCGTGAAATTc > 1:1631562/1‑71 (MQ=255)
aTGAAGCGAAGGATCTGGAATACCTCAACTCCAGCGTCAAAATCGTCAACCCGATTATGGGCGTGAAATTc > 1:1651561/1‑71 (MQ=255)
aTGAAGCGAAGAATCTGGAATACCTCAACTCCAGCGTCAAAATCGTCaa > 1:1408142/1‑49 (MQ=255)
aTGAAGCGAAGAATCTGGAATACCTCAACTCCAGCGTCAAAATCGTCaa > 1:491018/1‑49 (MQ=255)
aTGAAGCGAAGAATCTGGAATACCTCAACTCCAGCGTCAAAATCGTCaa > 1:1531974/1‑49 (MQ=255)
aTGAAGCGAAGAATCTGGAATACCTCAACTCCAGCGTCAAAATCGTCaa > 1:441695/1‑49 (MQ=255)
tGAAGCGAAGGATCTGGAATACGTCAACTCCAGCGTCAAAATCGTCAACCCGATTa > 1:3782180/1‑56 (MQ=255)
tGAAGCGAAGGATCTGGAATACCTCAACTCCAGCGTCAAAATCGTc < 1:291275/46‑1 (MQ=255)
tGAAGCGAAGGATCTGGAATACCTCAACTCCAGCGTCAAAATCGTCAACCCGATTa > 1:2270747/1‑56 (MQ=255)
tGAAGCGAAGAATCTGGAATACCTCAACTCCAGCGTCaaa < 1:47821/40‑1 (MQ=255)
tGAAGCGAAGAATCTGGAATACCTCAACTCCAGCGTCaaa < 1:2392283/40‑1 (MQ=255)
tGAAGCGAAGAATCTGGAATACCTCAACTCCAGCGTCaaa < 1:576838/40‑1 (MQ=255)
tGAAGCGAAGAATCTGGAATACCTCAACTCCAGCGTCaaa < 1:2017053/40‑1 (MQ=255)
tGAAGCGAAGAATCTGGAATACCTCAACTCCAGCGTCaaa < 1:4095308/40‑1 (MQ=255)
tGAAGCGAAGAATCTGGAATACCTCAACTCCAGCGTCaaa < 1:162653/40‑1 (MQ=255)
tGAAGCGAAGAATCTGGAATACCTCAACTCCAGCGTCaaa < 1:3309164/40‑1 (MQ=255)
aaGCGAAGGATCTGGAATACCTCAACTCCAGCGTCAAAATCGTCAACCCGa > 1:2336028/1‑51 (MQ=255)
aaGCGAAGGATCTGGAATACCTCAACTCCAGCGTCAAAATCGTCAACCCGATTATGGGCGTGAAATTCTgg > 1:334027/1‑71 (MQ=255)
aaGCGAAGGATCTGGAATACCTCAACTCCAGCGTCAAAATCGTCAACCCGATTATGGGCGTGAAATTCTgg < 1:1107149/71‑1 (MQ=255)
aaGCGAAGGATCTGGAATACCTCAACTCCAGCGTCAAAATCGTCAACCCGATTATGGGCGTGAAATTCTgg < 1:2027907/71‑1 (MQ=255)
aaGCGAAGGATCTGGAATACCTCAACTCCAGCGTCAAAATCGTCAACCCGATTATGGGCGTGAAATTCTgg < 1:3309709/71‑1 (MQ=255)
aaGCGAAGGATCTGGAATACCTCAACTCCAGCGTCAAAATCGTCAACCCGATTATGGGCGTGAAATTCTgg > 1:1498892/1‑71 (MQ=255)
aaGCGAAGAATCTGGAATACCTCAACTCCAGCGTCAAAATCGTCAACCCGATTATg > 1:1788434/1‑56 (MQ=255)
cGAAGGATCTTGAATACCTCAACTCCAGCGTCAAAATCGTCAACCCGATTATGGGCGTGaaa < 1:3155840/62‑1 (MQ=255)
gaagaaTCTGGAATACCTCAACTCCAGCGTCAAAATCGTCAACCCGATTATGGGCGTGAAAtt > 1:22370/1‑63 (MQ=255)
gAATGATCTGGAATACCTCAACTCCAGCGTCAAAATCGTCAACCCGATTATGGGCGTGAAATTCTGGGATg < 1:3223358/71‑1 (MQ=255)
gAAGGATCTGGAATACCTCAACTCCAGCGTCAAAATCGTCAACCCGATTATGGGCGTGAAATTCTGGGATg < 1:1026094/71‑1 (MQ=255)
gAAGGATCTGGAATACCTCAACTCCAGCGTCAAAATCGTCAACCCGATTATGGGAGTGAAATTCTGGGATg < 1:2694630/71‑1 (MQ=255)
aaGAATCTGGAATACCTCAACTCCAGCGTCAAAATCGTCAACCCGATTATGGGCGTGAAATTCTGGGATga < 1:3331910/71‑1 (MQ=255)
aGGATCTGGAATACCTCAACTCCAGCGTCAAAATCGTCAACCCGATTATGGGCGTGAAATTCTGGGAt > 1:231467/1‑68 (MQ=255)
aGGATCTGGAATACCTCAACTCCAGCGTCAAAATCGTCAACCAGATTATGGGCGTGAAATTCTGGGa > 1:2468287/1‑67 (MQ=255)
|
CAAAATGTCTGTCGAAAAAGCCTACTCCACAGACTCCAACATGCTTGGTGCAACGCATGAAGCGAAGGATCTGGAATACCTCAACTCCAGCGTCAAAATCGTCAACCCGATTATGGGCGTGAAATTCTGGGATGA > minE/2144278‑2144412
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
