Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F8 I0 R2
|
296 |
77.6 |
4260404 |
88.1% |
3753415 |
61.2 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
2,144,345 |
G→A |
45.0% |
D206N (GAT→AAT) |
argG → |
argininosuccinate synthetase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 2,144,345 | 0 | G | A | 45.0%
| 35.4
/ 123.6
| 91 | D206N (GAT→AAT) | argG | argininosuccinate synthetase |
| Reads supporting (aligned to +/- strand): ref base G (20/30); new base A (17/24); total (37/54) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
AATGTCTGTCGAAAAAGCCTACTCCACAGACTCCAACATGCTTGGTGCAACGCATGAAGCGAAGGATCTGGAATACCTCAACTCCAGCGTCAAAATCGTCAACCCGATTATGGGCGTGAAATTCTGGGAT > minE/2144281‑2144410
|
aaTGTCTGTCGAAAAAGCCTACTCCACAGACTCCAACATGCTTGGTGCAACGCATGAAGCGAAGAATCTgg < 1:2671956/71‑1 (MQ=255)
tctgtcGAAAAAGCCTACTCCACAGACTCCAACATGCTTGGTGCAACGCATGAAGCGAAGGATCTGGAATa < 1:3617213/71‑1 (MQ=255)
tcGAAAAAGCCTACTCCACAGACTCCAACATGCTTGGTGCAACGCATGAAGCGAAGGATCTgg < 1:2603356/63‑1 (MQ=255)
tcGAAAAAGCCTACTCCACAGACTCCAACATGCTTGGTGCAACGCATGAAGCGAAGAATCTgg < 1:2975273/63‑1 (MQ=255)
tcGAAAAAGCCTACTCCACAGACTCCAACATGCTTGGTGCAACGCATGAAGCGAAGAATCTgg < 1:459278/63‑1 (MQ=255)
tcGAAAAAGCCTACTCCACAGACTCCAACATGCTTGGTGCAACGCATGAAGCGAAGAATCTgg < 1:3809518/63‑1 (MQ=255)
gAAAAAGCCTACTCCACAGACTCCAACATGCTTGGTGCAACGCATGAAGCGAAGGATCTGGaa > 1:2020302/1‑63 (MQ=255)
aaaaaGCCTACTCCACAGACTCCAACATGCTTGGTGCAACGCATGAAGCGAAGGATc < 1:211703/57‑1 (MQ=255)
aaaaaGCCTACTCCACAGACTCCAACATGCTTGGTGCAACGCATGAAGCGAAGGATc < 1:3759460/57‑1 (MQ=255)
gCCTACTCCACAGACTCCAACATGCTTGGTGCAACGCATGAAGCGAAGAATCTgg < 1:922399/55‑1 (MQ=255)
tACTCCACAGACTCCAACATGCTTGGTGCAACGCATGAAGCGAAGGATCTGGAATACCTCAACTCCa < 1:3520407/67‑1 (MQ=255)
tACTCCACAGACTCCAACATGCTTGGTGCAACGCATGAAGCGAAGGATCTGGAATACCTCAACTCCa < 1:539660/67‑1 (MQ=255)
tACTCCACAGACTCCAACATGCTTGGTGCAACGCATGAAGCGAAGGATCTGGAATACCTCAACTCCa < 1:158298/67‑1 (MQ=255)
tCCACAGACTCCAACATGCTTGGTGCAACGCATGAAGCGAAGAATCTGGAATACCTCAACTCCAGCGTCa > 1:4065092/1‑70 (MQ=255)
tCCACAGACTCCAACATGCTTGGTGCAACGCATGAAGCGAAGAATCTGGAATACCTCAACTCCAGCGTCa > 1:2349126/1‑70 (MQ=255)
tCCACAGACTCCAACATGCTTGGTGCAACGCATGAAGCGAAGAATCTGGAATACCTCAACTCCAGCGTCa > 1:3036346/1‑70 (MQ=255)
cacaGACTCCAACATGCTTGGTGCAACGCATGAAGCGAAGGa < 1:2869574/42‑1 (MQ=255)
cacaGACTCCAACATGCTTGGTGCAACGCATGAAGCGAAGGATCTGGAATACCTCAACTCCAGCGTCaaa < 1:424499/70‑1 (MQ=255)
cacaGACTCCAACATGCTTGGTGCAACGCATGAAGCGAAGGATCTGGAATACCTCAACTCCAGCGTCaaa < 1:1210859/70‑1 (MQ=255)
cacaGACTACAACATGCTTGGTGCAACGCATGAAGCGAAGGATCTGGaa > 1:2342585/1‑49 (MQ=255)
acaGACTCCAACATGCTTGGTGCAACGCATGAAGCGAAGGATCTGGAATa > 1:1464837/1‑50 (MQ=255)
acaGACTCCAACATGCTTGGTGCAACGCATGAAGCGAAGGATCTGGAATAc > 1:1815530/1‑51 (MQ=255)
acaGACTCCAACATGCTTGGTGCAACGCATGAAGCGAAGAATCTGGAATACCTCAACTCCAGCGTCAAAAt > 1:3469066/1‑71 (MQ=255)
caGACTCCAACATGCTTGGTGCAACGCATGAAGCTAAGGATCTGGAATACCTCAACTCCAGCGTCAAAATc < 1:610878/71‑1 (MQ=255)
caGACTCCAACATGCTTGGTGCAACGCATGAAGCGAAGGATCTGGAATACCTCAACTCCAGCGTCAAAATc < 1:3779486/71‑1 (MQ=255)
aCTCCAACATGCTTGGTGCAACGCATGAAGCGAAGAATCTGGAATACCTCAACTCCAGCGTCaaa < 1:116041/65‑1 (MQ=255)
tCCAACATGCTTGGTGCAACGCATGAAGCGAAGGATCTgg > 1:2699081/1‑40 (MQ=255)
tCCAACATGCTTGGTGCAACGCATGAAGCGAAGGATCTgg > 1:2643810/1‑40 (MQ=255)
tCCAACATGCTTGGTGCAACGCATGAAGCGAAGGATCTgg > 1:980873/1‑40 (MQ=255)
tCCAACATGCTTGGTGCAACGCATGAAGCGAAGGATCTgg > 1:1177503/1‑40 (MQ=255)
tCCAACATGCTTGGTGCAACGCATGAAGCGAAGGATCTgg > 1:1279475/1‑40 (MQ=255)
tCCAACATGCTTGGTGCAACGCATGAAGCGAAGGATCTGGAATACCTCAACTCCAGCGTCAAAATc < 1:843391/66‑1 (MQ=255)
tCCAACATGCTTGGTGCAACGCATGAAGCGAAGGATCTGGAATACCTCAACTCCAGCGTCAAAATc < 1:2567271/66‑1 (MQ=255)
tCCAACATGCTTGGTGCAACGCATGAAGCGAAGGATCTGGAATACCTCAACTCCAGCGTCAAAATc < 1:160864/66‑1 (MQ=255)
tCCAACATGCTTGGTGCAACGCATGAAGCGAAGAATCTGGAATACCTCAACTCCAGCGTCaaaa > 1:2524624/1‑64 (MQ=255)
tCCAACATGCTTGGTGCAACGCATGAAGCGAAGAATCTGGAATACCTCAACTCCAGCGTCaaaa > 1:2540427/1‑64 (MQ=255)
tCCAACATGCTTGGTGCAACGCATGAAGCGAAGAATCTGGAATACCTCAACTCCAGCGTCaaaa > 1:2935372/1‑64 (MQ=255)
tCCAACATGCTTGGTGCAACGCATGAAGCGAAGAATCTGGAATACCTCAACTCCAGCGTCAAAAt > 1:1109877/1‑65 (MQ=255)
ccAACATGCTTGGTGCAACGCATGAAGCGAAGAATCTgg < 1:140316/39‑1 (MQ=255)
ccAACATGCTTGGTGCAACGCATGAAGCGAAGAATCTgg < 1:3730138/39‑1 (MQ=255)
ccAACATGCTTGGTGCAACGCATGAAGCGAAGAATCTgg < 1:358343/39‑1 (MQ=255)
ccAACATGCTTGGTGCAACGCATGAAGCGAAGAATCTgg < 1:2759927/39‑1 (MQ=255)
cAACATGCTTGGTGCAACGCATGAAGCGAAGGATCTGGAATACCTCAACTCCAGCGTCAAAATCGTCAAcc > 1:123452/1‑71 (MQ=255)
aaCATGCTTGGTGCAACGCATGAAGCGAAGAATCTGGAATACCTCAACTCCAGCGTCAAAATCGTCAAccc < 1:3319367/71‑1 (MQ=255)
aCATGCTTGGTGCAACGCATGAAGCGAAGAATCTGGAATACCTCaa > 1:2670236/1‑46 (MQ=255)
aTGCTTGGTGCAACGCATGAAGCGAAGGATCTGGAATACCTCAACTCCAGCGTCAAAATCGTCAACCCGAt < 1:505541/71‑1 (MQ=255)
aTGCTTGGTGCAACGCATGAAGCGAAGGATCTGGAATACCTCAACTCCAGCGTCAAAATCGTCAACCCGAt < 1:1616168/71‑1 (MQ=255)
tGCTTGGTGCAACGCATGAAGCGAAGAATCTGGAATa > 1:413737/1‑37 (MQ=255)
tGCTTGGTGCAACGCATGAAGCGAAGAATCTGGAATACCTCAACTCCAGCGTCAAAATCGTCAACCCGAtt < 1:3639102/71‑1 (MQ=255)
cTTGGTGCAACGCATGAAGCGAAGAATCTGGAATACCTCAACTCCAGCGTCAAAATCGTCAACCCGATTAt < 1:3135097/71‑1 (MQ=255)
ttGGTGCAACGCATGAAGCGAAGAATCTGGAATACCTCAACTCCAGCGTCAAAATCGTCAACCCGATTATg > 1:1360774/1‑71 (MQ=255)
ttGGTGAAACGCATGAAGCGAAGAATCTGGAATACCTCAACTCCAGCGTCAAAATCGTCAACCCGATTATg > 1:2854659/1‑71 (MQ=255)
tGGTGCAACGCATGAAGCGAAGAATCTGGAATACCTCAACTCCAGCGTCAAAATCGTCAACCCGATTATgg > 1:3176158/1‑71 (MQ=255)
tGGTGCAACGCATGAAGCGAAGAATCTGGAATACCTCAACTCCAGCGTCAAAATCGTCAACCCGATTATgg > 1:3248112/1‑71 (MQ=255)
ggTGCAACGCATGAAGCGAAGGATCTGGAATACCTCAACTCCAGCGTCAAAATCg < 1:3281305/55‑1 (MQ=255)
ggTGCAACGCATGAAGCGAAGGATCTGGAATACCTCAACTCCAGCGTCAAAATCg < 1:3670049/55‑1 (MQ=255)
ggTGCAACGCATGAAGCGAAGGATCTGGAATACCTCAACTCCAGCGTCAAAATCg < 1:2208329/55‑1 (MQ=255)
ggTGCAACGCATGAAGCGAAGGATCTGGAATACCTCAACTCCAGCGTCAAAATCg < 1:2651325/55‑1 (MQ=255)
ggTGCAACGCATGAAGCGAAGGATCTAGAATACCTCAACTCCAGCGTCAAAATCg < 1:811956/55‑1 (MQ=255)
ggTGCAACGCATGAAGCGAAGAATCTGGAATACCTCAACTCCAGCGTCAAAATCGTCAACCCGAtt > 1:3633810/1‑66 (MQ=255)
ggTGCAACGCATGAAGCGAAGAATCTGGAATACCTCAACTCCAGCGTCAAAATCGTCAACCCGATTATgg < 1:253528/70‑1 (MQ=255)
ggTGCAACGCATGAAGCGAAGAATCTGGAATACCTCAACTCCAGCGTCAAAATCGTCAACCCGATTATgg < 1:2348378/70‑1 (MQ=255)
gatGCAACGCATGAAGCGAAGGATCTGGAATACCTCAACTCCAGCGTCAAAATCg < 1:752698/53‑1 (MQ=255)
gTGCAACGCATGAAGCGAAGGATCTGGAATACCTCAACTCCAGCGTCAAAATCGTCAACCCGATTATGGGc < 1:1472408/71‑1 (MQ=255)
gTGCAACGCATGAAGCGAAGGATCTGGAATACCTCAACTCCAGCGTCAAAATCGTCAACCCGATTATGGGc < 1:1544375/71‑1 (MQ=255)
tGCAACGCATGAAGCGAAGGATCTGGAATACCTCAACTCCAGCGTCAAAATCGTCAACCCGATTAt < 1:4146528/66‑1 (MQ=255)
tGCAACGCATGAAGCGAAGGATCTGGAATACCTCAACTCCAGCGTCAAAATCGTCAACCCGATTAt < 1:3655767/66‑1 (MQ=255)
tGCAACGCATGAAGCGAAGGATCTGGAATACCTCAACTCCAGCGTCAAAATCGTCAACCCGATTAt < 1:1368956/66‑1 (MQ=255)
tGCAACGCATGAAGCGAAGGATCTGGAATACCTCAACTCCAGCGTCAAAATCGTCAACCCGATTAt < 1:3791085/66‑1 (MQ=255)
tGCAACGCATGAAGCGAAGGATCTGGAATACCTCAACTCCAGCGTCAAAATCGTCAACCCGATTAt < 1:387807/66‑1 (MQ=255)
gCAACGCATGAAGCGAAGGATCTGGAATACCTCAACTCCAGCGTCAAAATCGTCAACCCGATTATGGGCGt > 1:862464/1‑71 (MQ=255)
gCAACGCATGAAGCGAAGGATCTGGAATACCTCAACTCCAGCGTCAAAATCGTCAACCCGATTATGGGCGt > 1:4188067/1‑71 (MQ=255)
aCGCATGAAGCGAAGGATCTGGAATACCTCAACTCCAGCGTCAAAATCGTCAACCCGATTATGGGCGTg > 1:1368665/1‑69 (MQ=255)
aCGCATGAAGCGAAGGATCTGGAATACCTCAACTCCAGCGTCAAAATCGTCAACCCGATTATGGGCGTGa > 1:293130/1‑70 (MQ=255)
cATGAAGCGAAGAATCTGGAATACCTCAACTCCAGCGTCAAAATCGTCAACCCGATTATGGGCGTGAAAtt < 1:2684475/71‑1 (MQ=255)
cATGAAGCGAAGAATCTGGAATACCTCAACTCCAGCGTCAAAATCGTCAACCCGATTATGGGCGTGAAAtt < 1:208133/71‑1 (MQ=255)
cATGAAGCGAAGAATCTGGAATACCTCAACTCCAGCGTCAAAATCGTCAACCCGATTATGGGCGTGAAAtt < 1:2247840/71‑1 (MQ=255)
cATGAAGCGAAGAATCTGGAATACCTCAACTCCAGCGTCAAAATCGTCAACCCGATTATGGGCGTGAAAtt < 1:622440/71‑1 (MQ=255)
cATGAAGCGAAGAATCTGGAATACCTCAACTCCAGCGTCAAAATCGTCAACCCGATTATGGGCGTGAAAtt < 1:29189/71‑1 (MQ=255)
tGAAGCGAAGAATCTGGAATACCTCAACTCCAGCGTCAAAATCGTCAACCCGATTATGGGCGTGAAATTCt < 1:1926860/71‑1 (MQ=255)
tGAAGCGAAGAATCTGGAATACCTCAACTCCAGCGTCAAAATCGTCAACCCGATTATGGGCGTGAAATTCt < 1:4166984/71‑1 (MQ=255)
aaGCGAAGAATCTGGAATACCTCAACTCCAGCGTCAAAATCGTCAAccc < 1:2299597/49‑1 (MQ=255)
aaGCGAAGAATCTGGAATACCTCAACTCCAGCGTCAAAATCGTCAAccc < 1:1448471/49‑1 (MQ=255)
gCGAAGAATCTGGAATACCTCAACTCCAGCGTCAAAATCGTCAACCCGa > 1:133168/1‑49 (MQ=255)
cGAAGAATCTGGAATACCTCAACTCCAGCGTCAAAATCGTCAACCCGATTATGGGCGTGAAATTCTGGGAt > 1:3157783/1‑71 (MQ=255)
gaagaaTCTGGAATACCTCAACTCCAGCGTCAAAATCGTCAACCCGAt < 1:2124468/48‑1 (MQ=255)
gaagaaTCTGGAATACCTCAACTCCAGCGTCAAAATCGTCAACCCGAt < 1:495088/48‑1 (MQ=255)
gaagaaTCTGGAATACCTCAACTCCAGCGTCAAAATCGTCAACCCGAt < 1:2962342/48‑1 (MQ=255)
gaagaaTCTGGAATACCTCAACTCCAGCGTCAAAATCGTCAACCCGAt < 1:2698116/48‑1 (MQ=255)
gaagaaTCTGGAATACCTCAACTCCAGCGTCAAAATCGTCAACCCGAt < 1:1159370/48‑1 (MQ=255)
gaagaaTCTGGAATACCTCAACTCCAGCGTCAAAATCGTCAACCCGAt < 1:1836574/48‑1 (MQ=255)
aaGGATCTGGAATACCTCAACTCCAGCGTCAAAAt > 1:1265812/1‑35 (MQ=255)
aaGGATCTGGAATACCTCAACTCCAGCGTCAAAAt > 1:434778/1‑35 (MQ=255)
aaGGATCTGGAATACCTCAACTCCAGCGTCAAAAt > 1:3647628/1‑35 (MQ=255)
aaGGATCTGGAATACCTCAACTCCAGCGTCAAAAt > 1:3642615/1‑35 (MQ=255)
aaGGATCTGGAATACCTCAACTCCAGCGTCAAAAt > 1:1167476/1‑35 (MQ=255)
aaGGATCTGGAATACCTCAACTCCAGCGTCAAAAt > 1:2916938/1‑35 (MQ=255)
|
AATGTCTGTCGAAAAAGCCTACTCCACAGACTCCAACATGCTTGGTGCAACGCATGAAGCGAAGGATCTGGAATACCTCAACTCCAGCGTCAAAATCGTCAACCCGATTATGGGCGTGAAATTCTGGGAT > minE/2144281‑2144410
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
