Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F17 I0 R2
|
278 |
43.2 |
2215644 |
94.6% |
2095999 |
61.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
2,144,345 |
G→A |
13.3% |
D206N (GAT→AAT) |
argG → |
argininosuccinate synthetase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 2,144,345 | 0 | G | A | 13.3%
| 118.5
/ 9.1
| 45 | D206N (GAT→AAT) | argG | argininosuccinate synthetase |
| Reads supporting (aligned to +/- strand): ref base G (17/21); new base A (3/3); total (21/24) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
CTACAAAATGTCTGTCGAAAAAGCCTACTCCACAGACTCCAACATGCTTGGTGCAACGCATGAAGCGAAGGATCTGGAATACCTCAACTCCAGCGTCAAAATCGTCAACCCGATTATGGGCGTGAAATTCTGGGAT > minE/2144275‑2144410
|
cTACAAAATGTCTGTCGAAAAAGCCTACTCCACAGACTCCAACATGCTTGGTGCAACGCATGAAGCTAAgg < 1:1582816/71‑1 (MQ=255)
aaTGTCTGTCGAAAAAGCCTACTCCACAGACTCCAACATGCTTGGTGCAACGCATGAAGCGAAGGATCTg > 1:1546749/1‑70 (MQ=255)
aTGTCTGTCGAAAAAGCCTACTCCACAGACTCCAACATGCTTGGTGCAACGCATGAAGCGAAGGAt > 1:944713/1‑66 (MQ=255)
tcGAAAAAGCCTACTCCACAGCCTCCAACATGCTTGGTGCAACGCATGAAGCGAAGAATCTGGAATACCTc < 1:2184836/71‑1 (MQ=255)
tcGAAAAAGCCTACTCCACAGACTCCAACATGCTTGGTGCAACGCATGAAGCGAAGGATCTgg < 1:289380/63‑1 (MQ=255)
aaGCCTACTCCACAGACTCCAACATGCTTGGTGCAACGCATGAAGCGAAGGATCTGGAATACCTCAACTcc < 1:1648376/71‑1 (MQ=255)
cacaGACTCCAACATGCTTGGTGCAACGCATGAAGCGAAGGATCTGGaa > 1:1228614/1‑49 (MQ=255)
cacaGACTCCAACATGCTTGGTGCAACGCATGAAGCGAAGGATCTGGaa > 1:618925/1‑49 (MQ=255)
cacaGACTCCAACATGCTTGGTGCAACGCATGAAGCGAAGAATCTGGAATACCTCAACTCCAGCGTCaaaa > 1:949909/1‑71 (MQ=255)
cacaGACTCCAACATGCTTGGTGCAACGCATGAAGCGAAGAATCTGGAATACCTCAACTCCAGCGTCaaa < 1:1091290/70‑1 (MQ=255)
ccAACATGCTTGGTGCAACGCATGAAGCGAAGAATCTGGAATACCTc < 1:2185299/47‑1 (MQ=255)
cAACATGCTTGGTGCAACGCATGAAGCGAAGGATCTGGAATACCTCAACTCCa > 1:1424315/1‑53 (MQ=255)
cAACATGCTTGGTGCAACGCATGAAGCGAAGGATCTGGAATACCTCAACTCCa > 1:1453132/1‑53 (MQ=255)
cAACATGCTTGGTGCAACGCATGAAGCGAAGGATCTGGAATACCTCAACTCCAGCGTCAAAATCGTc > 1:280699/1‑67 (MQ=255)
aaCATGCTTGGTGCAACGCATGAAGCGAAGGATCTGGAATACCTCAACTCCAGCGTCAAAATCGTCAAccc > 1:498122/1‑71 (MQ=255)
aaCATGCTTGGTGCAACGCATGAAGCGAAGGATCAGGAATAc > 1:1287977/1‑42 (MQ=255)
aCATGCTTGGTGCAACGCATGAAGCGAAGGATCTGGAATACCTCAACTCCAGCGTCAAAATCGTCAAccc > 1:742278/1‑70 (MQ=255)
cATGCTTGGTGCAACGCATGAAGCGAAGAATCTGGAATACCTCAACTCCAGCGTCAAAATCGTCAACCCGa > 1:1097019/1‑71 (MQ=255)
aTGCTTGGTGCAACGCATGAAGCGAAGGATCTGGAATACCTCAACTCCAGCGTCAAAATCGTCAACCCGAt < 1:2125537/71‑1 (MQ=255)
aTGCTTGGTGCAACGCATGAAGCGAAGGATCTGGAATACCTCAACTCCAGCGTCAAAATCGTCAACCCGAt > 1:752234/1‑71 (MQ=255)
aTGCTTGGTGCAACGCATGAAGCGAAGGATCTGGAATACCTCAACTCCAGCGTCAAAATCGTCAACCCGAt < 1:253271/71‑1 (MQ=255)
tGCTTGGTGCAACGCATGAAGCGAAGGATCTGGAATACCTCAACTCCAGCGTCAAAATCGTCAACCCGAtt < 1:794976/71‑1 (MQ=255)
gCTTGGTGCAACGCATGAAGCGAAGGATCTGGAATACCTCAACTCCAGCGTCAAAATCGTCAACCCGATTa > 1:616734/1‑71 (MQ=255)
cTTGGTGCAACGCATGAAGCGAAGGATCTGGAATACCTCAACTCCAGCGTCAAAATCGTCAACCCGATTa < 1:1621110/70‑1 (MQ=255)
cTTGGTGCAACGCATGAAGCGAAGGATCTGGAATACCTCAACTCCAGCGTCAAAATCGTCAACCCGATTa < 1:723416/70‑1 (MQ=255)
cTTGGTGCAACGCATGAAGCGAAGGATCTGGAATACCTCAACTCCAGCGTCAAAATCGTCAACCCGATTa < 1:1099505/70‑1 (MQ=255)
cTTGGTGCAACGCATGAAGCGAAGGATCTGGAATACCTCAACTCCAGCGTCAAAATCGTCAACCCGATTa < 1:52543/70‑1 (MQ=255)
cTTGGTGCAACGCATGAAGCGAAGGATCTGGAATACCTCAACTCCAGCGTCAAAATCGTCAACCCGATTAt > 1:2138256/1‑71 (MQ=255)
gCAACGCATGAAGCGAAGTATCTGGAATACCTCAACTCCAGCGTCAAAATCGTCAACCCGATTATGGGCGt > 1:754317/1‑71 (MQ=255)
cAACGCATGAAGCGAAGGATCTGGAATACCTCAACTCCAGGGTCAAAATCGTCAACCCGATTATGGGCGt > 1:2080030/1‑70 (MQ=255)
aaCGCATGAAGCGAAGGATCTGGAATACCTCAACTCCAGCGTCAAAATCGTCAACCCGATTATGGGCGTGa < 1:2180675/71‑1 (MQ=255)
aaCGCATGAAGCGAAGGATCTGGAATACCTCAACTCCAGCGTCAAAATCGTCAACCCGATTATGGGCGTGa < 1:1383694/71‑1 (MQ=255)
aCGCATGAAGCGAAGGATCTGGAATACCTCAACTCCAGCGTCAAAATCGTCAACCCGATTATGGGCGTGa < 1:1014810/70‑1 (MQ=255)
aCGCATGAAGCGAAGAATCTGGAATACCTCAACTCCAGCGTCAAAATCGTCAACCCGATTATGGGCGTGa > 1:567252/1‑70 (MQ=255)
cGCATTAAGCGAAGGATCTGGAATACCTCAACTCCAGCGTCaaa < 1:1066384/44‑1 (MQ=255)
cGCATGAAGCGAAGGATCTGGAATACCTCAACTCCAGCGTCaaa < 1:2211165/44‑1 (MQ=255)
cGCATGAAGCGAAGGATCTGGAATACCTCAACTCCAGCGTCAAAATCGTCAACCCGATTATGGGCGTGaaa > 1:1241471/1‑71 (MQ=255)
cATGAAGCGAAGGATCTGGAATACCTCAACTCCAGCGTCAAAATCGTCAACCCGATTATGGGCGTGAAAtt < 1:767502/71‑1 (MQ=255)
aaGCGAAGGATCTGGAATACCTCAACTCCAGCGTCAAAATCGTCAACCCGATTATGTGCGTGAAATTCTgg > 1:1728584/1‑71 (MQ=255)
aaGCGAAGGATCTGGAATACCTCAACTCCAGCGTCAAAATCGTCAACCCGATTATGGGCGTGAAATTCTgg > 1:635623/1‑71 (MQ=255)
aaGCGAAGGATCTGGAATACCTCAACTCCAGCGTCAAAATCGTCAACCCGATTATGGGCGTGAAATTCTgg < 1:2133270/71‑1 (MQ=255)
aaGCGAAGGATCTGGAATACCTCAACTCCAGCGTCAAAATCGTCAACCCGATTATGGGCGTGAAATTCTgg < 1:1057634/71‑1 (MQ=255)
aaGCGAAGGATCTGGAATACCTCAACTCCAGCGTCAAAATCGTCAACCCGATTATGGGCGTGAAATTCTg < 1:482547/70‑1 (MQ=255)
cGAAGGATCTGGAATACCTCAACTCCAGCGTCAAAATCGTCAACCCGATTATGGGCGTGAAATTCTGGGAt < 1:1054398/71‑1 (MQ=255)
aaGGATCTGGAATACCTCAACTCCAGCGTCAAAATCGTCAACCCGATTATGGGCGTGAAATTCTgg < 1:726541/66‑1 (MQ=255)
aaGGATCTGGAATACCTCAACTCCAGCGTCAAAATCGTCAACCCGATTATGGGCGTGAAATTCTgg < 1:98755/66‑1 (MQ=255)
ggATCTGGAATACCTCAACTCCAGCGTCAAAATCGTCAACCCGATTATGGGCGTGAAATTCt > 1:968904/1‑62 (MQ=255)
|
CTACAAAATGTCTGTCGAAAAAGCCTACTCCACAGACTCCAACATGCTTGGTGCAACGCATGAAGCGAAGGATCTGGAATACCTCAACTCCAGCGTCAAAATCGTCAACCCGATTATGGGCGTGAAATTCTGGGAT > minE/2144275‑2144410
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
