Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F28 I1 R1
|
122 |
92.7 |
4382561 |
94.3% |
4132755 |
66.1 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
minE |
1,330,586 |
A→G |
Y334H (TAC→CAC) |
cpsG ← |
phosphomannomutase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 1,330,586 | 0 | A | G | 100.0%
| 166.1
/ NA
| 44 | Y334H (TAC→CAC) | cpsG | phosphomannomutase |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (13/31); total (13/31) |
ACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTAATGGTGGGCGCTCATTTCGCCACCATAGATGGCGTCTTCCTTGCGCATACGTTCTT > minE/1330522‑1330642
|
acCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTGAtggtg < 1:110420/71‑1 (MQ=255)
acCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTGAtggtg < 1:92175/71‑1 (MQ=255)
acCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTGAtggtg < 1:659648/71‑1 (MQ=255)
acCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTGAtggtg < 1:4118295/71‑1 (MQ=255)
acCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTGAtggtg < 1:3393807/71‑1 (MQ=255)
acCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTGAtggtg < 1:3228423/71‑1 (MQ=255)
acCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTGAtggtg < 1:2995200/71‑1 (MQ=255)
acCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTGAtggtg < 1:2798150/71‑1 (MQ=255)
acCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTGAtggtg < 1:1125220/71‑1 (MQ=255)
acCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTGAtggtg < 1:1453081/71‑1 (MQ=255)
gTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTGATGGTGGgcg < 1:835522/71‑1 (MQ=255)
gTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTGATGGTGGgcg < 1:589606/71‑1 (MQ=255)
gTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTGATGGTGGgcg < 1:538808/71‑1 (MQ=255)
gTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTGATGGTGGgcg < 1:3583347/71‑1 (MQ=255)
gTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTGATGGTGGgcg < 1:2420789/71‑1 (MQ=255)
gTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTGATGGTGGgcg < 1:1505248/71‑1 (MQ=255)
cagcCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTGATGGTGGGCGCTCATTTCGccac < 1:4050042/71‑1 (MQ=255)
cagcCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTGATGGTGGGCGCTCATTTCGccac < 1:3972550/71‑1 (MQ=255)
cagcCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTGATGGTGGGCGCTCATTTCGccac < 1:4327311/71‑1 (MQ=255)
cagcCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTGATGGTGGGCGCTCATTTCGccac < 1:2129553/71‑1 (MQ=255)
cACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTGATGGTGGGCGCTCATTTCGCCACCATa < 1:160346/71‑1 (MQ=255)
cACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTGATGGTGGGCGCTCATTTCGCCACCATa < 1:2367447/71‑1 (MQ=255)
cACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTGATGGTGGGCGCTCATTTCGCCACCATa < 1:3483988/71‑1 (MQ=255)
cACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTGATGGTGGGCGCTCATTTCGCCACCATa < 1:1499432/71‑1 (MQ=255)
cACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTGATGGTGGGCGCTCATTTCGCCACCATa < 1:3472198/71‑1 (MQ=255)
aTGCCGCTGTCGCAGTAAGCGAAATCACTGAAGTGAtggtg < 1:1047926/41‑1 (MQ=38)
gTAAGCGAAATCACGGAAGTGATGGTGGGCGCTCATTTCGCCACCATa < 1:4262708/48‑1 (MQ=255)
gTAAGCGAAATCACGGAAGTGATGGTGGGCGCTCATTTCGCCACCATAGATGGCGTCTTCCTTGCGCATAc > 1:113996/1‑71 (MQ=255)
tAAGCGAAATCACGTAAGTGATGGTGGGCGCTCATTTCGCCACCATAGATGGCGTCTTCCTTGCGCATACg < 1:2653178/71‑1 (MQ=255)
tAAGCGAAATCACGGAAGTGATGGTGGGCGCTCATTTCGCCACCATAGATGGCGTCTTCCTTGCGCATACg < 1:1967048/71‑1 (MQ=255)
tAAGCGAAATCACGGAAGTGATGGTGGGCGCTCATTTCGCCACCATAGATGGCGTCTTCCTTGCGCATACg < 1:2708622/71‑1 (MQ=255)
tAAGCGAAATCACGGAAGTGATGGTGGGCGCTCATTTCGCCACCATAGATGGCGTCTTCCTTGCGCATACg < 1:397612/71‑1 (MQ=255)
cGAAATCACGGAAGTGATGGTGGGCGCTCATTTCGCCACCATAGATGGCGTCTTCCTTGCGCATACGTTCt > 1:373178/1‑71 (MQ=255)
aaaTCACGGAAGTGATGGTGGGCGCTCATTTCGCCCCCATAGATGGCGTCTTCCTTGCGCATACGtt > 1:2067014/1‑67 (MQ=255)
aaaTCACGGAAGTGATGGTGGGCGCTCATTTCGCCACCATAGATGGCGTCtt > 1:3162627/1‑52 (MQ=255)
aaaTCACGGAAGTGATGGTGGGCGCTCATTTCGCCACCATAGATGGCGTCTTcc > 1:3690887/1‑54 (MQ=255)
aaaTCACGGAAGTGATGGTGGGCGCTCATTTCGCCACCATAGATGGCGTCTTCCTTgcg > 1:3035634/1‑59 (MQ=255)
aaaTCACGGAAGTGATGGTGGGCGCTCATTTCGCCACCATAGATGGCGTCTTCCTTgc > 1:3419601/1‑58 (MQ=255)
aaaTCACGGAAGTGATGGTGGGCGCTCATTTCGCCACCATAGATGGCGTCTTCCTTGCGCATACGTTCtt > 1:434340/1‑70 (MQ=255)
aaaTCACGGAAGTGATGGTGGGCGCTCATTTCGCCACCATAGATGGCGTCTTCCTTGCGCATACGTTCtt > 1:2326734/1‑70 (MQ=255)
aaaTCACGGAAGTGATGGTGGGCGCTCATTTCGCCACCATAGATGGCGTCTTCCTTGCGCATACGTTCtt > 1:623966/1‑70 (MQ=255)
aaaTCACGGAAGTGATGGTGGGCGCTCATTTCGCCACCATAGATGGCGTCTTCCTTGCGCATACGTTCtt > 1:1933229/1‑70 (MQ=255)
aaaTCACGGAAGTGATGGTGGGCGCTCATTTCGCCACCATAGATGGCGTCTTCCTTGCGCATACGTTCtt > 1:1862053/1‑70 (MQ=255)
aaaTCACGGAAGTGATGGTGGGCGCTCATATCGCCACCAt > 1:2635342/1‑40 (MQ=38)
|
ACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTAATGGTGGGCGCTCATTTCGCCACCATAGATGGCGTCTTCCTTGCGCATACGTTCTT > minE/1330522‑1330642
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
