Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F28 I3 R2
|
122 |
64.1 |
3058059 |
97.6% |
2984665 |
63.3 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
minE |
1,330,586 |
A→G |
Y334H (TAC→CAC) |
cpsG ← |
phosphomannomutase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 1,330,586 | 0 | A | G | 100.0%
| 187.5
/ NA
| 53 | Y334H (TAC→CAC) | cpsG | phosphomannomutase |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (28/25); total (28/25) |
CACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTAATGGTGGGCGCTCATTTCGCCACCATAGATGGCGTCTTCCTTGCGCATACGTTCTTTAATAAAGGC > minE/1330521‑1330652
|
cacCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTGAtggt < 1:799603/71‑1 (MQ=255)
acCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTGAtggtg > 1:2864469/1‑71 (MQ=255)
gTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTGATGGTGGgcg > 1:1193981/1‑71 (MQ=255)
cGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTGATGGTGGGCGCt < 1:1152723/70‑1 (MQ=255)
ggCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTGATGGTGGGCGCTCa < 1:993239/71‑1 (MQ=255)
gACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTGATGGTGGGCGCTCAttt > 1:2158525/1‑71 (MQ=255)
aCCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTGATGGTGGGCGCTCATTTc < 1:2660703/71‑1 (MQ=255)
cagcagCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTGAtggtgg > 1:476146/1‑58 (MQ=255)
cagcagCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTGAtggtgg > 1:414498/1‑58 (MQ=255)
cagcagCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTGATGGTGGGCGCTCATTTCg > 1:1764593/1‑70 (MQ=255)
gcagcCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTGAtggtg > 1:2190203/1‑55 (MQ=255)
gcagcCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTGATGGTGGGCGCt < 1:1098244/61‑1 (MQ=255)
gcagcCACGGGATAATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTGATGGTGGGCGCTCATTTCGcca > 1:2413402/1‑71 (MQ=255)
ccACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTGATGGTGGGCGCTc < 1:931233/58‑1 (MQ=255)
cACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTGATGGTGGGCGCTCATTTCg > 1:1895607/1‑63 (MQ=255)
aCGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTGATGGTggg < 1:1821639/51‑1 (MQ=255)
aCGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTGATGGTGGGCGCTCATTTCGccacca > 1:1359389/1‑68 (MQ=255)
cGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTGATGGTGGGCGCTCATTTc > 1:735036/1‑60 (MQ=255)
cGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTGATGGTGGGCGCTCATTTCGCCACCATAGa < 1:861307/71‑1 (MQ=255)
gggATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTGAtggt < 1:1823690/46‑1 (MQ=255)
gggATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTGAtggt > 1:656461/1‑46 (MQ=255)
gggATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTGAtggt < 1:2444077/46‑1 (MQ=255)
gggATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTGATGGTGGGCGCTCATTTCGCCACCATa < 1:2703631/68‑1 (MQ=255)
gATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTGATGGTGGGCGCTCATTTCGCCACCATAGATg < 1:2370809/70‑1 (MQ=255)
aTCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTGATGGTGGGCGCTCATTTCGcc < 1:287265/59‑1 (MQ=255)
aTCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTGATGGTGGGCGCTCATTTCGCCACCATAGa < 1:2274188/67‑1 (MQ=255)
aTCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTGATGGTGGGCGCTCATTTCGCCACCATAGa < 1:2277823/67‑1 (MQ=255)
aTGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTGAtggt > 1:1939817/1‑40 (MQ=255)
aTGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTGATGGTGGGCGCTCATTTCGCCACCATAGATGGCGTc > 1:103007/1‑71 (MQ=255)
cGCTGTCGCAGTAAGCGAAATCACGGAAGTGATGGTGGGCGCTCATTTCGCCACCATAGATGGCGTCTTcc > 1:1199849/1‑71 (MQ=255)
cTGTCGCAGTAAGCGAAATCACGGAAGTGATGGTGGGCGCTCATTTCGCCACCATAGATGGCGTCTTCCtt > 1:1913089/1‑71 (MQ=255)
cTGTCGCAGTAAGCGAAATCACGGAAGTGATGGTGGGCGCTCATTTCGCCACCATAGATGGCGTCTTCCtt > 1:1974885/1‑71 (MQ=255)
tGTCGCAGTAAGCGAAATCACGGAAGTGGTGGTGGGCGCTCATTTCGCCACCATAGATgg > 1:235280/1‑60 (MQ=255)
gCAGTAAGCGAAATCACGGAAGTGATGGTGGGCGCTCATTTCGCCACCATAGAt < 1:1563325/54‑1 (MQ=255)
gCAGTAAGCGAAATCACGGAAGTGATGGTGGGCGCTCATTTCGCCACCATAGATGGCGTCTTCCTTGCGCa > 1:2352954/1‑71 (MQ=255)
gTAAGCGAAATCACGGAAGTGATGGTGGGCGCTCATTTCGcca < 1:2259143/43‑1 (MQ=255)
gTAAGCGAAATCACGGAAGTGATGGTGGGCGCTCATTTCGCCACCATAGATGGCGTCTTCCTTGCGCATAc > 1:1120117/1‑71 (MQ=255)
tAAGCGAAATCACGGAAGTGATGGTGGGCGCTCATTTCGCCACCATAGa > 1:2206223/1‑49 (MQ=255)
aaGCGAAATCACGGAAGTGATGGTGGGCGCTCAttt > 1:2693349/1‑36 (MQ=255)
aaGCGAAATCACGGAAGTGATGGTGGGCGCTCATTTCGCCACCATAGATGGCGTCTTCCTTGCGCATACGt > 1:1231962/1‑71 (MQ=255)
aGCGAAATCACGGAAGTGATGGTGGGCGCTCATTTCGCCACCATAGATGGCg < 1:1866338/52‑1 (MQ=255)
aGCGAAATCACGGAAGTGATGGTGGGCGCTCATTTCGCCACCATAGATGGCGTCTTCCTTGCGCATACGtt < 1:2520231/71‑1 (MQ=255)
gCGAAATCACGGAAGTGATGGTGGGCGCTCATTTCGCCACCATAGATGGCGTCTTCCTTGCGCATACGTTc < 1:2139707/71‑1 (MQ=255)
gCGAAATCACGGAAGTGATGGTGGGCGCTCATTTCGCCACCATAGATGGCGTCTTCCTTGCGCATACGTTc < 1:1899744/71‑1 (MQ=255)
aaaTCACGGAAGTGATGGTGGGCGCTCATTTCGCCACCATAGATGGCGTCTTCCTTGCGCATACGTTCtt > 1:1586661/1‑70 (MQ=255)
aCGGAAGTGATGGTGGGCGCTCATTTCGCCACCATa < 1:586964/36‑1 (MQ=255)
cGGAAGTGATGGTGGGCGCTCATTTCGCCACCATAGATGGCGTCTTCCTTGCGCATACGTTCTTTAATaaa < 1:2166191/71‑1 (MQ=255)
ggAAGTGATGGTGGGCGCTCATTTCGCCACCATAGATg > 1:1522041/1‑38 (MQ=255)
ggAAGTGATGGTGGGCGCTCATTTCGCCACCATAGATGGCg < 1:3026379/41‑1 (MQ=255)
ggAAGTGATGGTGGGCGCTCATTTCGCCACCATAGATGGCGTCTTCCTTGCGCATACGTTCTTTAATAAAg > 1:2533392/1‑71 (MQ=255)
gAAGTGATGGTGGGCGCTCATTTCGCCACCATAGATGGCGTCTTCCTTGCGCATACGTTCt < 1:1332782/61‑1 (MQ=255)
aaGTGATGGTGGGCGCTCATTTCGCCACCATAGATGGCGTCTTCCTTGCGCATACGTTCTTTAATAAAGGc > 1:608598/1‑71 (MQ=255)
aaGTGATGGTGGGCGCTCATTTCGCCACCATAGATGGCGTCTTCCTTGCGCATACGTTCTTTAATAAAGGc > 1:1564363/1‑71 (MQ=255)
|
CACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTAATGGTGGGCGCTCATTTCGCCACCATAGATGGCGTCTTCCTTGCGCATACGTTCTTTAATAAAGGC > minE/1330521‑1330652
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
