Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A2 F28 I1 R1 122 92.7 4382561 94.3% 4132755 66.1

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA minE 1,643,860 A→G R9R (CGT→CGC)  purC ← phosphoribosylaminoimidazole‑succinocarboxamide synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE1,643,8600AG100.0% 541.4 / NA 142R9R (CGT→CGC) purCphosphoribosylaminoimidazole‑succinocarboxamide synthetase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (91/51);  total (91/51)

Only 100 of 142 total aligned reads displayed.

ATTGCGGAATTCGAGCACCAACAGGTCCGGGTTTTCCGTGCTGTATACGGTTTTCGCTTTACCACGATACAACTCAGCTTGCTTTTGCATCTTTATCACTCCTGGGTGTGAATTAACGTTTTAAAA  >  minE/1643797‑1643922
                                                               |                                                              
aTTGCGGAATTCGAGCACCAACAGGTCCGGGTTTTCCGTGCTGTATACGGTTTTCGCTTTACCGCGATAc                                                          <  1:906841/70‑1 (MQ=255)
aTTGCGGAATTCGAGCACCAACAGGTCCGGGTTTTCCGTGCTGTATACGGTTTTCGCTTTACCGCGATAc                                                          <  1:2245344/70‑1 (MQ=255)
aTTGCGGAATTCGAGCACCAACAGGTCCGGGTTTTCCGTGCTGTATACGGTTTTCGCTTTACCGCGATAc                                                          <  1:162049/70‑1 (MQ=255)
aTTGCGGAATTCGAGCACCAACAGGTCCGGGTTTTCCGTGCTGTATACGGTTTTCGCTTTACCGCGATAc                                                          <  1:4010026/70‑1 (MQ=255)
aTTGCGGAATTCGAGCACCAACAGGTCCGGGTTTTCCGTGCTGTATACGGTTTTCGCTTTACCGCGATAc                                                          <  1:1391821/70‑1 (MQ=255)
      gAATTCGAGCACCAACAGGTCCGGGTTTTCCGTGCTGTATACGGTTTTCGCTTTACCGCGATACaa                                                        >  1:2651991/1‑66 (MQ=255)
      gAATTCGAGCACCAACAGGTCCGGGTTTTCCGTGCTGTATACGGTTTTCGCTTTACCGCGATACAACTCAg                                                   >  1:1376019/1‑71 (MQ=255)
      gAATTCGAGCACCAACAGGTCCGGGTTTTCCGTGCTGTATACGGTTTTCGCTTTACCGCGATACAACTCAg                                                   >  1:2226549/1‑71 (MQ=255)
      gAATTCGAGCACCAACAGGTCCGGGTTTTCCGTGCTGTATACGGTTTTCGCTTTACCGCGATACAACTCAg                                                   >  1:249051/1‑71 (MQ=255)
      gAATTCGAGCACCAACAGGTCCGGGTTTTCCGTGCTGTATACGGTTTTCGCTTTACCGCGATACAACTCAg                                                   >  1:3015430/1‑71 (MQ=255)
      gAATTCGAGCACCAACAGGTCCGGGTTTTCCGTGCTGTATACGGTTTTCGCTTTACCGCGATACAACTCAg                                                   >  1:3306753/1‑71 (MQ=255)
      gAATTCGAGCACCAACAGGTCCGGGTTTTCCGTGCTGTATACGGTTTTCGCTTTACCGCGATACAACTCAg                                                   >  1:3377407/1‑71 (MQ=255)
      gAATTCGAGCACCAACAGGTCCGGGTTTTCCGTGCTGTATACGGTTTTCGCTTTACCGCGATACAACTCAg                                                   >  1:943065/1‑71 (MQ=255)
              gCACCAACAGGTCCGGGTTTTCCGTGCTGTATACGGTTTTCGCTTTACCGCGATACAACTCAGCTTGCttt                                           >  1:3456735/1‑71 (MQ=255)
              gCACCAACAGGTCCGGGTTTTCCGTGCTGTATACGGTTTTCGCTTTACCGCGATACAACTCAGCTTGCttt                                           >  1:3285037/1‑71 (MQ=255)
              gCACCAACAGGTCCGGGTTTTCCGTGCTGTATACGGTTTTCGCTTTACCGCGATACAACTCAGCTTGCttt                                           >  1:3135938/1‑71 (MQ=255)
              gCACCAACAGGTCCGGGTTTTCCGTGCTGTATACGGTTTTCGCTTTACCGCGATACAACTCAGCTTGCttt                                           >  1:3795412/1‑71 (MQ=255)
              gCACCAACAGGTCCGGGTTTTCCGTGCTGTATACGGTTTTCGCTTTACCGCGATACAACTCAGCTTGCttt                                           >  1:2692717/1‑71 (MQ=255)
              gCACCAACAGGTCCGGGTTTTCCGTGCTGTATACGGTTTTCGCTTTACCGCGATACAACTCAGCTTGCttt                                           >  1:4075174/1‑71 (MQ=255)
              gCACCAACAGGTCCGGGTTTTCCGTGCTGTATACGGTTTTCGCTTTACCGCGATACAACTCAGCTTGCttt                                           >  1:736084/1‑71 (MQ=255)
               cACCAACAGGTCCGGGTTTTCCGTGCTGTATACGGTTTTCGCTTTACCGCGATACAACTCAGCTTGCttt                                           <  1:811204/70‑1 (MQ=255)
                aCCAACAGGTCCGGGTTTTCCGTGCTGTATACGGTTTTCGCTTTACCGCGATACAACTCAGCTTGCTTTTg                                         <  1:3069845/71‑1 (MQ=255)
                aCCAACAGGTCCGGGTTTTCCGTGCTGTATACGGTTTTCGCTTTACCGCGATACAACTCAGCTTGCTTTTg                                         <  1:3407963/71‑1 (MQ=255)
                aCCAACAGGTCCGGGTTTTCCGTGCTGTATACGGTTTTCGCTTTACCGCGATACAACTCAGCTTGCTTTTg                                         <  1:3976656/71‑1 (MQ=255)
                 ccAACAGGTCCGGGTTTTCCGTGCTGTATACGGTTTTCGCTTTACCGCGATACAACTCAGCTTGCTTTTg                                         >  1:1129530/1‑70 (MQ=255)
                 ccAACAGGTCCGGGTTTTCCGTGCTGTATACGGTTTTCGCTTTACCGCGATACAACTCAGCTTGCTTTTg                                         >  1:793860/1‑70 (MQ=255)
                 ccAACAGGTCCGGGTTTTCCGTGCTGTATACGGTTTTCGCTTTACCGCGATACAACTCAGCTTGCTTTTg                                         >  1:1779435/1‑70 (MQ=255)
                 ccAACAGGTCCGGGTTTTCCGTGCTGTATACGGTTTTCGCTTTACCGCGATACAACTCAGCTTGCTTTTg                                         >  1:3537237/1‑70 (MQ=255)
                 ccAACAGGTCCGGGTTTTCCGTGCTGTATACGGTTTTCGCTTTACCGCGATACAACTCAGCTTGCTTTTg                                         >  1:2581549/1‑70 (MQ=255)
                 ccAACAGGTCCGGGTTTTCCGTGCTGTATACGGTTTTCGCTTTACCGCGATACAACTCAGCTTGCTTTTGc                                        <  1:1834129/71‑1 (MQ=255)
                 ccAACAGGTCCGGGTTTTCCGTGCTGTATACGGTTTTCGCTTTACCGCGATACAACTCAGCTTGCTTTTGc                                        <  1:1057348/71‑1 (MQ=255)
                 ccAACAGGTCCGGGTTTTCCGTGCTGTATACGGTTTTCGCTTTACCGCGATACAACTCAGCTTGCTTTTGc                                        <  1:3027479/71‑1 (MQ=255)
                 ccAACAGGTCCGGGTTTTCCGTGCTGTATACGGTTTTCGCTTTACCGCGATACAACTCAGCTTGCTTTTGc                                        <  1:1388927/71‑1 (MQ=255)
                 ccAACAGGTCCGGGTTTTCCGTGCTGTATACGGTTTTCGCTTTACCGCGATACAACTCAGCTTGCTTTTGc                                        <  1:4317088/71‑1 (MQ=255)
                 ccAACAGGTCCGGGTTTTCCGTGCTGTATACGGTTTTCGCTTTACCGCGATACAACTCAGCTTGCTTTTGc                                        <  1:3685260/71‑1 (MQ=255)
                 ccAACAGGTCCGGGTTTTCCGTGCTGTATACGGTTTTCGCTTTACCGCGATACAACTCAGCTTGCTTTTGc                                        <  1:2516330/71‑1 (MQ=255)
                 ccAACAGGTCCGGGTTTTCCGTGCTGTATACGGTTTTCGCTTTACCGCGATACAACTCAGCTTGCTTTTGc                                        <  1:3637990/71‑1 (MQ=255)
                 ccAACAGGTCCGGGTTTTCCGTGCTGTATACGGTTTTCGCTTTACCGCGATACAACTCAGCTTGCTTTTGc                                        <  1:158866/71‑1 (MQ=255)
                            gggTTTTCCGTGCTGTATACGGTTTTCGCTTTACCGCGATACAACTCAGCTTGCTTTTGc                                        <  1:1566064/60‑1 (MQ=255)
                            gggTTTTCCGTGCTGTATACGGTTTTCGCTTTACCGCGATACAACTCAGCTTGCTTTTGc                                        <  1:3409794/60‑1 (MQ=255)
                            gggTTTTCCGTGCTGTATACGGTTTTCGCTTTACCGCGATACAACTCAGCTTGCTTTTGc                                        <  1:2644210/60‑1 (MQ=255)
                            gggTTTTCCGTGCTGTATACGGTTTTCGCTTTACCGCGATACAACTCAGCTTGCTTTTGc                                        <  1:2559849/60‑1 (MQ=255)
                            gggTTTTCCGTGCTGTATACGGTTTTCGCTTTACCGCGATACAACTCAGCTTGCTTTTGc                                        <  1:2225648/60‑1 (MQ=255)
                            gggTTTTCCGTGCTGTATACGGTTTTCGCTTTACCGCGATACAACTCAGCTTGCTTTTGc                                        <  1:4750/60‑1 (MQ=255)
                            gggTTTTCCGTGCTGTATACGGTTTTCGCTTTACCGCGATACAACTCAGCTTGCTTTTGCATCTTTATCAc                             <  1:3352358/71‑1 (MQ=255)
                            gggTTTTCCGTGCTGTATACGGTTTTCGCTTTACCGCGATACAACTCAGCTTGCTTTTGCATCTTTATCAc                             <  1:4356570/71‑1 (MQ=255)
                            gggTTTTCCGTGCTGTATACGGTTTTCGCTTTACCGCGATACAACTCAGCTTGCTTTTGCATCTTTATCAc                             <  1:103959/71‑1 (MQ=255)
                            gggTTTTCCGTGCTGTATACGGTTTTCGCTTTACCGCGATACAACTCAGCTTGCTTTTGCATCTTTATCAc                             <  1:3433455/71‑1 (MQ=255)
                             ggTTTTCCGTGCTGTATACGGTTTTCGCTTTACCGCGATACAACTCAGCTTACTTTTGCATCTTTATCAc                             <  1:4103006/70‑1 (MQ=255)
                                tttCCGTGCTGTATACGGTTTTCGCTTTACCGCGATAc                                                          <  1:1587000/38‑1 (MQ=255)
                                 ttCCGTGCTGTATACGGTTTTCGCTTTACCGCGATACAACTCAGCTTGCTTTTGCATCTTTATCACTCCTg                        >  1:3798019/1‑71 (MQ=255)
                                 ttCCGTGCTGTATACGGTTTTCGCTTTACCGCGATACAACTCAGCTTGCTTTTGCATCTTTATCACTCCTg                        >  1:1846364/1‑71 (MQ=255)
                                 ttCCGTGCTGTATACGGTTTTCGCTTTACCGCGATACAACTCAGCTTGCTTTTGCATCTTTATCACTCCTg                        >  1:2838424/1‑71 (MQ=255)
                                 ttCCGTGCTGTATACGGTTTTCGCTTTACCGCGATACAACTCAGCTTGCTTTTGCATCTTTATCACTCCTg                        >  1:45592/1‑71 (MQ=255)
                                      tGCTGTTTACGGTTTTCGCTTTACCGCGATACAACTCAGCTTGCTTTTGCATCTTTAt                                >  1:1248/1‑58 (MQ=255)
                                      tGCTGTATTCGGTTTTCGCTTTACCGCGATACAACTCAGCTTGCTTTTGCATCTTTAt                                >  1:18955/1‑58 (MQ=255)
                                      tGCTGTATACGGTTTTCGCTTTACCGCGATACAACTCAGCTTGCTTTTGCATCttt                                  >  1:892549/1‑56 (MQ=255)
                                      tGCTGTATACGGTTTTCGCTTTACCGCGATACAACTCAGCTTGCTTTTGCATCtt                                   >  1:3112403/1‑55 (MQ=255)
                                      tGCTGTATACGGTTTTCGCTTTACCGCGATACAACTCAGCTTGCTTTTGCATCTTTAt                                >  1:4140262/1‑58 (MQ=255)
                                      tGCTGTATACGGTTTTCGCTTTACCGCGATACAACTCAGCTTGCTTTTGCATCTTTAt                                >  1:4205391/1‑58 (MQ=255)
                                      tGCTGTATACGGTTTTCGCTTTACCGCGATACAACTCAGCTTGCTTTTGCATCTTTAt                                >  1:127438/1‑58 (MQ=255)
                                      tGCTGTATACGGTTTTCGCTTTACCGCGATACAACTCAGCTTGCTTTTGCATCTTTAt                                >  1:3695925/1‑58 (MQ=255)
                                      tGCTGTATACGGTTTTCGCTTTACCGCGATACAACTCAGCTTGCTTTTGCATCTTTAt                                >  1:1976434/1‑58 (MQ=255)
                                      tGCTGTATACGGTTTTCGCTTTACCGCGATACAACTCAGCTTGCTTTTGCATCTTTAt                                >  1:2592372/1‑58 (MQ=255)
                                      tGCTGTATACGGTTTTCGCTTTACCGCGATACAACTCAGCTTGCTTTTGCATCTTTAt                                >  1:3592608/1‑58 (MQ=255)
                                      tGCTGTATACGGTTTTCGCTTTACCGCGATACAACTCAGCTTGCTTTTGCATCTTTAt                                >  1:654167/1‑58 (MQ=255)
                                      tGCTGTATACGGTTTTCGCTTTACCGCGATACAACTCAGCTTGCTTTTGCATCTTTAt                                >  1:625837/1‑58 (MQ=255)
                                      tGCTGTATACGGTTTTCGCTTTACCGCGATACAACTCAGCTTGCTTTTGCATCTTTAt                                >  1:3475723/1‑58 (MQ=255)
                                      tGCTGTATACGGTTTTCGCTTTACCGCGATACAACTCAGCTTGCTTTTGCATCTTTAt                                >  1:274932/1‑58 (MQ=255)
                                      tGCTGTATACGGTTTTCGCTTTACCGCGATACAACTCAGCTTGCTTTTGCATCTTTAt                                >  1:1320325/1‑58 (MQ=255)
                                      tGCTGTATACGGTTTTCGCTTTACCGCGATACAACTCAGCTTGCTTTTGCATCTTTAt                                >  1:2821632/1‑58 (MQ=255)
                                      tGCTGTATACGGTTTTCGCTTTACCGCGATACAACTCAGCTTGCTTTTGCATCTTTAt                                >  1:3312108/1‑58 (MQ=255)
                                      tGCTGTATACGGTTTTCGCTTTACCGCGATACAACTCAGCTTGCTTTTGCATCTTTAt                                >  1:932493/1‑58 (MQ=255)
                                      tGCTGTATACGGTTTTCGCTTTACCGCGATACAACTCAGCTTGCTTTTGCATCTTTAt                                >  1:3175874/1‑58 (MQ=255)
                                      tGCTGTATACGGTTTTCGCTTTACCGCGATACAACTCAGCTTGCTTTTGCATCTTTAt                                >  1:1420697/1‑58 (MQ=255)
                                      tGCTGTATACGGTTTTCGCTTTACCGCGATACAACTCAGCTTGCTTTTGCATCTTTAt                                >  1:2944820/1‑58 (MQ=255)
                                      tGCTGTATACGGTTTTCGCTTTACCGCGATACAACTCAGCTTGCTTTTGCATCTTTAt                                >  1:2941755/1‑58 (MQ=255)
                                      tGCTGTATACGGTTTTCGCTTTACCGCGATACAACTCAGCATGCTTTTGCATCTTTAt                                >  1:1051596/1‑58 (MQ=255)
                                               cGGTTTTCGCTTTACCGCGATACAACTCAGCTTGCTTTTGCATCTTTATCACTCCTGGGTGTGAATTAACg          >  1:1330655/1‑71 (MQ=255)
                                               cGGTTTTCGCTTTACCGCGATACAACTCAGCTTGCTTTTGCATCTTTATCACTCCTGGGTGTGAATTAACg          >  1:789493/1‑71 (MQ=255)
                                               cGGTTTTCGCTTTACCGCGATACAACTCAGCTTGCTTTTGCATCTTTATCACTCCTGGGTGTGAATTAACg          >  1:3529424/1‑71 (MQ=255)
                                               cGGTTTTCGCTTTACCGCGATACAACTCAGCTTGCTTTTGCATCTTTATCACTCCTGGGTGTGAATTAACg          >  1:1287573/1‑71 (MQ=255)
                                                 gTTTTCGCTTTACCGCGATACAACTCAGCTTGCTTTTGCATCTTTATCACTc                           >  1:3196082/1‑52 (MQ=255)
                                                 gTTTTCGCTTTACCGCGATACAACTCAGCTTGCTTTTGCATCTTTATCACTCCTGGGTGTGaa                >  1:3873570/1‑63 (MQ=255)
                                                 gTTTTCGCTTTACCGCGATACAACTCAGCTTGCTTTTGCATCTTTATCACTCCTGGGTGTGAATTAACGtt        >  1:12517/1‑71 (MQ=255)
                                                 gTTTTCGCTTTACCGCGATACAACTCAGCTTGCTTTTGCATCTTTATCACTCCTGGGTGTGAATTAACGtt        >  1:850942/1‑71 (MQ=255)
                                                 gTTTTCGCTTTACCGCGATACAACTCAGCTTGCTTTTGCATCTTTATCACTCCTGGGTGTGAATTAACGtt        >  1:188912/1‑71 (MQ=255)
                                                 gTTTTCGCTTTACCGCGATACAACTCAGCTTGCTTTTGCATCTTTATCACTCCTGGGTGTGAATTAACGtt        >  1:1135398/1‑71 (MQ=255)
                                                 gTTTTCGCTTTACCGCGATACAACTCAGCTTGCTTTTGCATCTTTATCACTCCTGGGTGTGAATTAACGtt        >  1:366883/1‑71 (MQ=255)
                                                 gTTTTCGCTTTACCGCGATACAACTCAGCTTGCTTTTGCATCTTTATCACTCCTGGGTGTGAATTAACGtt        >  1:4349033/1‑71 (MQ=255)
                                                       gCTTTACCGCGATACAACTCAGCTTGCTTTTGCATCTTTATCACTCCTGGGTGTGAATTAACGTTTTaaaa  >  1:650671/1‑71 (MQ=255)
                                                       gCTTTACCGCGATACAACTCAGCTTGCTTTTGCATCTTTATCACTCCTGGGTGTGAATTAACGTTTTaaaa  >  1:74432/1‑71 (MQ=255)
                                                       gCTTTACCGCGATACAACTCAGCTTGCTTTTGCATCTTTATCACTCCTGGGTGTGAATTAACGTTTTaaaa  >  1:1621059/1‑71 (MQ=255)
                                                       gCTTTACCGCGATACAACTCAGCTTGCTTTTGCATCTTTATCACTCCTGGGTGTGAATTAACGTTTTaaaa  >  1:1819684/1‑71 (MQ=255)
                                                       gCTTTACCGCGATACAACTCAGCTTGCTTTTGCATCTTTATCACTCCTGGGTGTGAATTAACGTTTTaaaa  >  1:1992648/1‑71 (MQ=255)
                                                       gCTTTACCGCGATACAACTCAGCTTGCTTTTGCATCTTTATCACTCCTGGGTGTGAATTAACGTTTTaaaa  >  1:811820/1‑71 (MQ=255)
                                                       gCTTTACCGCGATACAACTCAGCTTGCTTTTGCATCTTTATCACTCCTGGGTGTGAATTAACGTTTTaaaa  >  1:1754384/1‑71 (MQ=255)
                                                       gCTTTACCGCGATACAACTCAGCTTGCTTTTGCATCTTTATCACTCCTGGGTGTGAATTAACGTTTTaaaa  >  1:1159863/1‑71 (MQ=255)
                                                       gCTTTACCGCGATACAACTCAGCTTGCTTTTGCATCTTTATCACTCCTGGGTGTGAATTAACGTTTTaaa   >  1:1411248/1‑70 (MQ=255)
                                                       gCTTTACCGCGATACAACTCAGCTTGCTTTTGCATCTTTATCACTCCTGGGTGTGAATTAACGTTTTaaa   >  1:2841465/1‑70 (MQ=255)
                                                               |                                                              
ATTGCGGAATTCGAGCACCAACAGGTCCGGGTTTTCCGTGCTGTATACGGTTTTCGCTTTACCACGATACAACTCAGCTTGCTTTTGCATCTTTATCACTCCTGGGTGTGAATTAACGTTTTAAAA  >  minE/1643797‑1643922

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A