Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F17 I0 R2
|
278 |
43.2 |
2215644 |
94.6% |
2095999 |
61.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
1,643,860 |
A→G |
26.8% |
R9R (CGT→CGC) |
purC ← |
phosphoribosylaminoimidazole‑succinocarboxamide synthetase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 1,643,860 | 0 | A | G | 26.8%
| 82.7
/ 32.5
| 51 | R9R (CGT→CGC) | purC | phosphoribosylaminoimidazole‑succinocarboxamide synthetase |
| Reads supporting (aligned to +/- strand): ref base A (13/24); new base G (7/6); total (21/30) |
| Fisher's exact test for biased strand distribution p-value = 3.27e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
CGTATCATTGCGGAATTCGAGCACCAACAGGTCCGGGTTTTCCGTGCTGTATACGGTTTTCGCTTTACCACGATACAACTCAGCTTGCTTTTGCATCTTTATCACTCCTGGGTGTGAATTAACGTTTTAAAATCTT > minE/1643791‑1643926
|
cGTATCATTGCGGAATTCGAGCACCAACAGGTCCGGGTTTTCCGTGCTGTATACGGTTTTCGCTTTACCAc < 1:1588269/71‑1 (MQ=255)
gTATCATTGCGGAATTCGAGCACCAACAGGTCCGGGTTTTCCGTGCTGTATACGGTTTTCGCTTTACcgcg < 1:1861304/71‑1 (MQ=255)
gTATCATTGCGGAATTCGAGCACCAACAGGTCCGGGTTTTCCGTGCTGTATACGGTTTTCGCTTTACCAc < 1:1117565/70‑1 (MQ=255)
tATCATTGCGGAATTCGAGCACCAACAGGTCCGGGTTTTCCGTGCTGTATACGGTTTTCGCTTTACCACGa > 1:1332969/1‑71 (MQ=255)
tCATTGCGGAATTCGAGCACCAACAGGTCCGGGTTTTCCGTGCTGTATACGGTTTTCGCTTTACcgcg < 1:363138/68‑1 (MQ=255)
aTTGCGGAATTCGAGCACCAACAGGTCCGGGTTTTCCGTGCTGTATACGGTTTTCGCTTTACCACGATAc > 1:798737/1‑70 (MQ=255)
aTTGCGGAATTCGAGCACCAACAGGTCCGGGTTTTCCGTGCTGTATACGGTTTTCGCTTTACCACGATAc < 1:1863382/70‑1 (MQ=255)
aTTGCGGAATTCGAGCACCAACAGGTCCGGGTTTTCCGTGCTGTATACGGTTTTCGCTTTACCACGATAc > 1:313957/1‑70 (MQ=255)
aTTCGAGCACCAACAGGTCCGGGTTTTCCGTTCTGTATACGGTTTTCGCTTTACCACg < 1:952215/58‑1 (MQ=255)
aTTCGAGCACCAACAGGTCCGGGTTTTCCGTGCTGTATACGGTTTTCGCTTTACCACg < 1:471719/58‑1 (MQ=255)
aGCACCAACAGGTCCGGGTTTTCCGTGCTGTATACGGTTTTCGCTTTACCACGATACAACTCAgcttgc < 1:646647/69‑1 (MQ=255)
gCACCAACAGGTCCGGGTTTTCCGTGCTGTATACGGTTTTCGCTTTACCACGATACAACTCAGCTTGCttt > 1:1270773/1‑71 (MQ=255)
gCACCAACAGGTCCGGGTTTTCCGTGCTGTATACGGTTTTCGCTTTACCACGATACAACTCAGCTTGCttt > 1:1549920/1‑71 (MQ=255)
cAACAGGTCCGGGTTTTCCGTGCTGTATACGGTTTTCGCTTTACCAc < 1:863743/47‑1 (MQ=255)
cAACAGGTCCGGGTTTTCCGTGCTGTATACGGTTTTCGCTTTACCACGATACAACTCAgcttgct < 1:1534442/65‑1 (MQ=255)
cAACAGGTCCGGGTTTTCCGTGCTGTATACGGTTTTCGCTTTACCACGATACAACTCAGCTTGCTTTTGCa < 1:2142210/71‑1 (MQ=255)
cAACAGGTCCGGGTTTTCCGTGCTGTATACGGTTTTCGCTTTACCACGATACAACTCAGCTTGCTTTTGCa < 1:1141701/71‑1 (MQ=255)
cAACAGGTCCGGGTTTTCCGTGCTGTATACGGTTTTCGCTTTACCACGATACAACTCAGCTTGCTTTTGCa < 1:1716930/71‑1 (MQ=255)
aaCAGGTCCGGGTTTTCCGTGCTGTATACGGTTTTCGCTTTACCGCGATACa > 1:1023249/1‑52 (MQ=255)
aaCAGGTCCGGGTTTTCCGTGCTGTATACGGTTTTCGCTTTACCACGa > 1:613048/1‑48 (MQ=255)
aaCAGGTCCGGGTTTTCCGTGCTGTATACGGTTTTCGCTTTAC‑‑CGATACAACTCAGCTTGCTTTTGCATCt > 1:1515664/1‑71 (MQ=255)
aCAGGTCCGGGTTTTCCGTGCTGTATACGGTTTTCGCTTTACCGCGATACAACTCAGCTTGCTTTTGCAt > 1:483284/1‑70 (MQ=255)
aGGTCCGGGTTTTCCGTGCTGTATACGGTTTTCGCTTTACCACGATACAACTCAGCTTGCTTTTGCATCtt < 1:1754235/71‑1 (MQ=255)
tCCGGGTTTTCCGTGCTGTATACGGTTTTCGCTTTACCGCGATACAACTCAGCTTGCTTTTGCATCTTTAt < 1:2091631/71‑1 (MQ=255)
tCCGGGTTTTCCGTGCTGTATACGGTTTTCGCTTTACCACGATACAACTCAGCTTGCTTTTGCATCTTTAt < 1:1124471/71‑1 (MQ=255)
gTTTTCCGTGCTGTATACGGTTTTCGCTTTACCACGATACAACTCAgc < 1:222922/48‑1 (MQ=255)
ttttCCGTGCTGTATACGGTTTTCGCTTTACCACGATACAACTCAGCTTGCTTTTGCATCt < 1:839773/61‑1 (MQ=255)
tttCCGTGCTGTATACGGTTTTCGCTTTACCACGATACAACTCAGCTTGCtttt > 1:1849371/1‑54 (MQ=255)
ttCCGTACTGTATACGGTTTTCGCTTTACCGCGATACAACTCAGCTTGCTTTTGCATCTTTATCACTCCTg > 1:282281/1‑71 (MQ=255)
cGTGCTGTATACGGTTTTCGCTTTACCACGATACAACTCAGCTTGCTTTTGCATCTTTAt < 1:325915/60‑1 (MQ=255)
tGCTGTATACGGTTTTCGCTTTACCGCGTTACAACTCAgcttg < 1:1661025/43‑1 (MQ=38)
cTGTATACGGTTTTCGCTTTACCGCGATACAACTCAGCTTGCTTTTGCATCTTTATCACTCCTGGGtgtg < 1:2062144/70‑1 (MQ=255)
tataCGGTTTTCGCTTTACCACGATACAACTCAGCTTGCTTTTGCATCTTTATCACTCCTGGGTGTGAAtt < 1:225346/71‑1 (MQ=255)
taCGGTTTTCGCTTTACCGCGATACAACTCAGCTTGCTTTTGCATCTTTATCACTCCTGGGTGTGAATTaa > 1:382649/1‑71 (MQ=255)
taCGGTTTTCGCTTTACCACGATACAACTCAGCTTGCTTTTGCATc < 1:1410156/46‑1 (MQ=255)
taCGGTTTTCGCTTTACCACGATACAACTCAGCTTGCTTTTGCATCTTTATCACTCCTGGGTGTGAAtt < 1:1386275/69‑1 (MQ=255)
aCGGTTTTCGCTTTACCACGATACAACTCAGCTTGCTTTTGCATCTTTATCACTCCTGGGTGTGAATTAAc > 1:2093117/1‑71 (MQ=255)
cGGTTTTCGCTTTACCGCGATACAACTCAGCTTGCTTTTGCATCTTTATCa > 1:844622/1‑51 (MQ=255)
cGGTTTTCGCTTTACCGCGATACAACTCAGCTTGCTTTTGCATCTTTATCa > 1:742207/1‑51 (MQ=255)
cGGTTTTCGCTTTACCACGATACAACTCAgcttgct < 1:2174388/36‑1 (MQ=255)
cGGTTTTCGCTTTACCACGATACAACTCAgcttgct < 1:38920/36‑1 (MQ=255)
cGGTTTTCGCTTTACCACGATACAACTCAgcttgct < 1:1242199/36‑1 (MQ=255)
cGGTTTTCGCTTTACCACGATACAACTCAGCTTGCTTTTGCATCTTTATCACTCCTGGGTGTGaa > 1:1368447/1‑65 (MQ=255)
cGGTTTTCGCTTTACCACGATACAACTCAGCTTGCTTTTGCATCTTTATCACTCCTGGGTGTGAATTAAc > 1:1648400/1‑70 (MQ=255)
cGGTTTTAGCTTTACCGCGATACAACTCAGCTTGCTTTTGCATCTTTAt > 1:1408593/1‑49 (MQ=39)
ggTTTTCGCTTTACCACGATACAACTCAGCTTGCTTTTGc < 1:2183515/40‑1 (MQ=255)
ttttCGCTTTACCACGATACAACTCAGCTTGCTTTTGCATCTTTATCACTCCTGGGTGTTAATTAACGttt > 1:989222/1‑71 (MQ=255)
ttCGCTTTACCACGATACAACTCAGCTTGCTTTTGCATCTTTATc > 1:2061398/1‑45 (MQ=255)
tCGCTTTACCGCGATACAACTCAGCTTGCTTTTGCATCTTTATCACTCCTGGGTGTGAATTAACGTTTTa < 1:717983/70‑1 (MQ=255)
tttACCGCGATACAACTCAGCTTGCTTTTGCATCTTTATCACTcc < 1:2201408/45‑1 (MQ=255)
tACCGCGATACAACTCAGCTTGCTTTTGCATCTTTATCACTCCTGGGTGTGAATTAACGTTTTAAAATCt < 1:1057296/70‑1 (MQ=255)
tACCACGATACAACTCAGCTTGCTTTTGCATCTTTATCACTCCTGGGTGTGAATTAACGTTTTAAAATc < 1:560663/69‑1 (MQ=255)
tACCACGATACAACTCAGCTTGCTTTTGCATCTTTATCACTCCTGGGTGTGAATTAACGTTTTAAAATCtt > 1:1957350/1‑71 (MQ=255)
|
CGTATCATTGCGGAATTCGAGCACCAACAGGTCCGGGTTTTCCGTGCTGTATACGGTTTTCGCTTTACCACGATACAACTCAGCTTGCTTTTGCATCTTTATCACTCCTGGGTGTGAATTAACGTTTTAAAATCTT > minE/1643791‑1643926
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
