Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F3 I0 R2
|
260 |
44.3 |
3360226 |
90.5% |
3041004 |
64.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
W3110S.gb |
921,273 |
G→A |
36.5% |
D169N (GAT→AAT) |
macB → |
fused macrolide transporter subunits and ATP‑binding component and membrane component of ABC superfamily |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 921,273 | 0 | G | A | 36.5%
| 47.9
/ 49.9
| 52 | D169N (GAT→AAT) | macB | fused macrolide transporter subunits and ATP‑binding component and membrane component of ABC superfamily |
| Reads supporting (aligned to +/- strand): ref base G (11/22); new base A (10/9); total (21/31) |
| Fisher's exact test for biased strand distribution p-value = 2.42e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01 |
AGCGCGTCAGCATCGCGCGGGCATTGATGAACGGTGGTCAGGTAATTCTTGCCGATGAACCAACCGGCGCACTGGACAGCCATTCTGGCGAAGAGGTGATGGCGATCCTGCATC > W3110S.gb/921220‑921333
|
aGCGCGTCAGCATCGCGCGGGCATTGATGATCGGTGGTCAGGTAATTCTTGCCAATGAACCAACCGGCGCa > 1:808907/1‑71 (MQ=255)
aGCGCGTCAGCATCGCGCGGGCATTGATGAACGGTGGTCAGGTAATTCTTGCCGATGAACCAGCCGGCGCa > 1:3250123/1‑71 (MQ=255)
aGCGCGTCAGCATCGCGCGGGCATTGATGAACGGTGGTCAGGTAATTCTTGCCGATGAACCAACCGGCGCa > 1:493258/1‑71 (MQ=255)
aGCGCGTCAGCATCGCGCGGGCATTGATGAACGGTGGTCAGGTAATTCTTGCCGATGAACCAACCGGCGCa > 1:441435/1‑71 (MQ=255)
aGCGCGTCAGCATCGCGCGGGCATTGATGAACGGTGGTCAGGTAATTCTTGCCAATGAACCAACCGgcgaa > 1:22136/1‑69 (MQ=255)
aGCGCGTCAGCATCGCGCGGGCATTGATGAACGGTGGTCAGGTAATTCTTGCCAATGAACCAACCGGCGCa > 1:1206659/1‑71 (MQ=255)
aGCGCGTCAGCATCGCGCGGGCATTGATGAACGGTGGTCAGGTAATTCTTGCCAATGAACCAACCGGCGCa > 1:372535/1‑71 (MQ=255)
aGCGCGTCAGCATCGCGCGGGCATTGATGAACGGTGGTCAGGTAATTCTTGCCAATGAACCAACCGGCGCa > 1:330625/1‑71 (MQ=255)
aGCGCGTCAGCATCGCGCGGGCATTGATGAACGGTGGTCAGGTAATTCTTGCCAATGAACCAACCGGCGCa > 1:2760856/1‑71 (MQ=255)
aGCGCGTCAGCATCGCGCGGGCATTGATGAACGGTGGTCAGGTAATTCTTGCCAATGAACCAACCGGCGCa > 1:2597939/1‑71 (MQ=255)
aGCGCGTCAGCATCGCGCGGGCATTGATGAACGGTGGTCAGGTAATTCTTGCCAATGAACCAACCGGCGCa > 1:2569770/1‑71 (MQ=255)
aGCGCGTCAGCATCGCGCGGGCATTGATGAACGGTGGTCAGGTAATTCTTGCCAATGAACCAACCGGCGCa > 1:2029744/1‑71 (MQ=255)
aGCGCGTCAGCATCGCGCGGGCATTGATGAACGGTGGTCAGGTAATTCTTGCCAATGAACCAACCGGCGCa > 1:1909386/1‑71 (MQ=255)
aGCGCGTCAGCATCGCGCGGGCATTGATGAACGGTGGTCAGGTAATTCTTGACGATGaaccaaa > 1:2074113/1‑63 (MQ=255)
aGCATCGCGCGGGCATTGATGAACGGTGGTCAGGTAATTCTTGCCAATGAACCAACCGGCGCACTGGACa < 1:920439/70‑1 (MQ=255)
aGCATCGCGCGGGCATTGATGAACGGTGGTCAGGTAATTCTTGCCAATGAACCAACCGGCGCACTGGACa < 1:1655771/70‑1 (MQ=255)
aGCATCGCGCGGGCATTGATGAACGGTGGTCAGGTAATTCTTGCCAATGAACCAACCGGCGCACTGGACa < 1:320987/70‑1 (MQ=255)
aGCATCGCGCGGGCATTGATGAACGGTGGTCAGGTAATTCTTGCCAATGAACCAACCGGCGCACTGGACa < 1:1785571/70‑1 (MQ=255)
aGCATCGCGCGGGCATTGATGAACGGTGGTCAGGTAATTCTTGCCAATGAACCAACCGGCGCACTGGACa < 1:2498139/70‑1 (MQ=255)
aGCATCGCGCGGGCATTGATGAACGGTGGTCAGGTAATTCTTGCCAATGAACCAACCGGCGCACTGGACa < 1:1507089/70‑1 (MQ=255)
aGCATCGCGCGGGCATTGATGAACGGTGGTCAGGTAATTCTTGCCAATGAACCAACCGGCGCACTGGACa < 1:1295787/70‑1 (MQ=255)
gCATCGCGCGGGCATTGATGAACGGTGGTCAGGTATTTCTTGCCAATGAACCAACCGGCGCACTGGACa < 1:2503284/69‑1 (MQ=255)
ggCATTGATGAACGGTGGTCAGGTAATTCTTGCCAATGAACCAACCGGCGCACTGGACa < 1:1892482/59‑1 (MQ=255)
atgaACGGTGGTCAGGTAATTCTTGCCGATGAACCAACCGGCGCACTGGACAGCCATTCTGGCGAAGAGGt < 1:3338033/71‑1 (MQ=255)
atgaACGGTGGTCAGGTAATTCTTGCCGATGAACCAACCGGCGCACTGGACAGCCATTCTGGCGAAGAGGt < 1:3339676/71‑1 (MQ=255)
atgaACGGTGGTCAGGTAATTCTTGCCGATGAACCAACCGGCGCACTGGACAGCCATTCTGGCGAAGAGGt < 1:1356140/71‑1 (MQ=255)
atgaACGGTGGTCAGGTAATTCTTGCCGATGAACCAACCGGCGCACTGGACAGCCATTCTGGCGAAGAGGt < 1:1971398/71‑1 (MQ=255)
atgaACGGTGGTCAGGTAATTCTTGCCGATGAACCAACCGGCGCACTGGACAGCCATTCTGGCGAAGAGGt < 1:2827319/71‑1 (MQ=255)
tgaACGGTGGTCAGGTAATTCTTGCCGATGAACCAACCGGCGCACTGGACAGCCATTCTGGCGAAGAGGt < 1:2676628/70‑1 (MQ=255)
tgaACGGTGGTCAGGTAATTCTTGCCGATGAACCAACCGGCGCACTGGACAGCCATTCTGGCGAAGAGGt < 1:2680510/70‑1 (MQ=255)
gTAATTCTTGCCGATGAACCAACCGGCGCACTGGACAGCCATTCTGGCGAAGAGGt < 1:1594235/56‑1 (MQ=255)
gTAATTCTTGCCGATGAACCAACCGGCGCACTGGACAGCCATTCTGGCGAAGAGGt < 1:281810/56‑1 (MQ=255)
gTAATTCTTGCCGATGAACCAACCGGCGCACTGGACAGCCATTCTGGCGAAGAGGt < 1:174696/56‑1 (MQ=255)
gTAATTCTTGCCGATGAACCAACCGGCGCACTGGACAGCCATTCTGGCGAAGAGGt < 1:2435103/56‑1 (MQ=255)
gTAATTCTTGCCGATGAACCAACCGGCGCACTGGACAGCCATTCTGGCGAAGAGGt < 1:2807976/56‑1 (MQ=255)
aTTCTTGCCGATGAACCAACCGGCGCACTGGACAGCCATTCTGGCGAAGAGGTGATGGCGATCCTGCATc < 1:2519317/70‑1 (MQ=255)
ttCTTGCCGATGAACCAACCGGCGCACTGGACAGCCATTCTGGCGAAGAGGt < 1:1903782/52‑1 (MQ=255)
ttCTTGCCGATGAACCAACCGGCGCACTGGACAGCCATTCTGGCGAAGAGGt < 1:890416/52‑1 (MQ=255)
ttCTTGCCGATGAACCAACCGGCGCACTGGACAGCCATTCTGGCGAAGAGGt < 1:1097281/52‑1 (MQ=255)
ttCTTGCCGATGAACCAACCGGCGCACTGGACAGCCATTCTGGCGAAGAGGt < 1:3310474/52‑1 (MQ=255)
ttCTTGCCGATGAACCAACCGGCGCACTGGACAGCCATTCTGGCGAAGAGGt < 1:1823098/52‑1 (MQ=255)
ttCTTGCCGATGAACCAACCGGCGCACTGGACAGCCATTCTGGCGAAGAGGt < 1:187680/52‑1 (MQ=255)
ttCTTGCCGATGAACCAACCGGCGCACTGGACAGCCATTCTGGCGAAGAGGt < 1:445348/52‑1 (MQ=255)
ttCTTGCCGATGAACCAACCGGCGCACTGGACAGCCATTCTGGCGAAGAGGt < 1:58438/52‑1 (MQ=255)
tacTTGCCGATGAACCAACCGGCGCACTGGACAGCCATTCTGGCGAAGAGGt < 1:1252613/50‑1 (MQ=255)
cctGCCGATGAACCAACCGGCGCACTGGACAGCCATTCTgg > 1:2314852/3‑41 (MQ=255)
cTTGCCGATGAACCAACCGGCGCACTGGACAGCCATTCTGGCGaa > 1:1787326/1‑45 (MQ=255)
cTTGCCGATGAACCAACCGGCGCACTGGACAGCCATTCTGGCGaa > 1:873617/1‑45 (MQ=255)
cTTGCCGATGAACCAACCGGCGCACTGGACAGCCATTCTGGCGaa > 1:3277843/1‑45 (MQ=255)
cTTGCCGATGAACCAACCGGCGCACTGGACAGCCATTCTGGCGaa > 1:3248341/1‑45 (MQ=255)
cTTGCCGATGAACCAACCGGCGCACTGGACAGCCATTCTGGCGaa > 1:3047346/1‑45 (MQ=255)
cTTGCCGATGAACCAACCGGCGCACTGGACAGCCATTCTGGCGaa > 1:2677135/1‑45 (MQ=255)
|
AGCGCGTCAGCATCGCGCGGGCATTGATGAACGGTGGTCAGGTAATTCTTGCCGATGAACCAACCGGCGCACTGGACAGCCATTCTGGCGAAGAGGTGATGGCGATCCTGCATC > W3110S.gb/921220‑921333
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
