Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F28 I2 R1
|
330 |
0.0 |
2544031 |
91.9% |
2337964 |
62.1 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
1,249,660 |
A→C |
Y344* (TAT→TAG) |
dhaH ← |
fused predicted dihydroxyacetone‑specific PTS enzyme HPr component and EI component |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 1,249,660 | 0 | A | C | 100.0%
| 20.2
/ NA
| 7 | Y344* (TAT→TAG) | dhaH | fused predicted dihydroxyacetone‑specific PTS enzyme HPr component and EI component |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base C (0/7); total (0/7) |
CATCATCCAGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCATATTCTGCCGTGCAA > W3110S.gb/1249605‑1249675
|
catcatCCAGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCCTATTCTGcc < 1:992868/65‑1 (MQ=255)
ccAGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCCTATTCTGCCGTGCaa < 1:1011239/65‑1 (MQ=255)
ccAGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCCTATTCTGCCGTGCaa < 1:1066123/65‑1 (MQ=255)
ccAGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCCTATTCTGCCGTGCaa < 1:2412037/65‑1 (MQ=255)
ccAGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCCTATTCTGCCGTGCaa < 1:2461312/65‑1 (MQ=255)
ccAGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCCTATTCTGCCGTGCaa < 1:2504306/65‑1 (MQ=255)
ccAGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCCTATTCTGCCGTGCaa < 1:409480/65‑1 (MQ=255)
|
CATCATCCAGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCATATTCTGCCGTGCAA > W3110S.gb/1249605‑1249675
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
