Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F28 I1 R1
|
354 |
41.7 |
3110465 |
93.1% |
2895842 |
61.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
1,249,660 |
A→C |
Y344* (TAT→TAG) |
dhaH ← |
fused predicted dihydroxyacetone‑specific PTS enzyme HPr component and EI component |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 1,249,660 | 0 | A | C | 100.0%
| 101.5
/ NA
| 29 | Y344* (TAT→TAG) | dhaH | fused predicted dihydroxyacetone‑specific PTS enzyme HPr component and EI component |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base C (0/29); total (0/29) |
CCAGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCATATTCTGCCGTGCAA > W3110S.gb/1249611‑1249675
|
ccAGTTGCTGGTATTTCTGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCCTATTCTGCCGTGCaa < 1:861269/65‑1 (MQ=255)
ccAGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCCTATTCTGCCGTGCaa < 1:2177343/65‑1 (MQ=255)
ccAGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCCTATTCTGCCGTGCaa < 1:975947/65‑1 (MQ=255)
ccAGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCCTATTCTGCCGTGCaa < 1:870696/65‑1 (MQ=255)
ccAGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCCTATTCTGCCGTGCaa < 1:855983/65‑1 (MQ=255)
ccAGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCCTATTCTGCCGTGCaa < 1:792553/65‑1 (MQ=255)
ccAGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCCTATTCTGCCGTGCaa < 1:2788317/65‑1 (MQ=255)
ccAGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCCTATTCTGCCGTGCaa < 1:2511589/65‑1 (MQ=255)
ccAGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCCTATTCTGCCGTGCaa < 1:2510282/65‑1 (MQ=255)
ccAGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCCTATTCTGCCGTGCaa < 1:242914/65‑1 (MQ=255)
ccAGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCCTATTCTGCCGTGCaa < 1:2412921/65‑1 (MQ=255)
ccAGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCCTATTCTGCCGTGCaa < 1:2397632/65‑1 (MQ=255)
ccAGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCCTATTCTGCCGTGCaa < 1:2373497/65‑1 (MQ=255)
ccAGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCCTATTCTGCCGTGCaa < 1:2367971/65‑1 (MQ=255)
ccAGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCCTATTCTGCCGTGCaa < 1:2338115/65‑1 (MQ=255)
ccAGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCCTATTCTGCCGTGCaa < 1:1113665/65‑1 (MQ=255)
ccAGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCCTATTCTGCCGTGCaa < 1:2140209/65‑1 (MQ=255)
ccAGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCCTATTCTGCCGTGCaa < 1:2137071/65‑1 (MQ=255)
ccAGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCCTATTCTGCCGTGCaa < 1:1742522/65‑1 (MQ=255)
ccAGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCCTATTCTGCCGTGCaa < 1:1592463/65‑1 (MQ=255)
ccAGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCCTATTCTGCCGTGCaa < 1:1564305/65‑1 (MQ=255)
ccAGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCCTATTCTGCCGTGCaa < 1:1556118/65‑1 (MQ=255)
ccAGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCCTATTCTGCCGTGCaa < 1:1471319/65‑1 (MQ=255)
ccAGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCCTATTCTGCCGTGCaa < 1:1461144/65‑1 (MQ=255)
ccAGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCCTATTCTGCCGTGCaa < 1:1337391/65‑1 (MQ=255)
ccAGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCCTATTCTGCCGTGCaa < 1:131001/65‑1 (MQ=255)
ccAGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCCTATTCTGCCGTGCaa < 1:1286835/65‑1 (MQ=255)
ccAGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCCTATTCTGCCGTGCaa < 1:1140439/65‑1 (MQ=255)
aGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCCTATTCTGCCGTGCaa < 1:1231154/63‑1 (MQ=255)
|
CCAGTTGCTGGTATTGCTGGCTAAGTTCTTTAAGAACTTGCTGCCAGGCATATTCTGCCGTGCAA > W3110S.gb/1249611‑1249675
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
