Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F28 I2 R1
|
330 |
0.0 |
2544031 |
91.9% |
2337964 |
62.1 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
3,767,300 |
A→G |
I166V (ATA→GTA) |
dgoA → |
2‑oxo‑3‑deoxygalactonate 6‑phosphate aldolase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 3,767,300 | 0 | A | G | 100.0%
| 12.5
/ NA
| 5 | I166V (ATA→GTA) | dgoA | 2‑oxo‑3‑deoxygalactonate 6‑phosphate aldolase |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (2/3); total (2/3) |
TCGGACATCGCAGTCTTTGCCGTTGGCGGCGTGACGCCAGAAAACCTGGCGCAGTGGATAGACGCAGGTTGTGCAGGGGCGGGCTTAGGCAGCGATCTCTATCGCGCCG > W3110S.gb/3767243‑3767351
|
tCGGACATCGCAGTCTTTGCCGTTGGCGGCGTGACGCCAGAAAACCTGGCGCAGTGGGTAGACGc > 1:1186382/1‑65 (MQ=255)
tCGGACATCGCAGTCTTTGCCGTTGGCGGCGTGACGCCAGAAAACCTGGCGCAGTGGGTAGACGc > 1:1569484/1‑65 (MQ=255)
ccTGGCGCAGTGGGTAGACGCAGGTTGTGCAGGGGCGGGCTTAGGCAGCGATCTCTATCGCGCCg < 1:1324183/65‑1 (MQ=255)
ccTGGCGCAGTGGGTAGACGCAGGTTGTGCAGGGGCGGGCTTAGGCAGCGATCTCTATCGCGCCg < 1:548131/65‑1 (MQ=255)
ccTGGCGCAGTGGGGAGACGCAGGTTGTGCAGGGGCGGGCTTAGGCAGCGATCTCTATCGCGCCg < 1:1051377/65‑1 (MQ=255)
|
TCGGACATCGCAGTCTTTGCCGTTGGCGGCGTGACGCCAGAAAACCTGGCGCAGTGGATAGACGCAGGTTGTGCAGGGGCGGGCTTAGGCAGCGATCTCTATCGCGCCG > W3110S.gb/3767243‑3767351
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
