Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F28 I3 R1
|
178 |
99.0 |
1887089 |
95.3% |
1798395 |
63.2 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
2,810,107 |
+A |
coding (25/1173 nt) |
emrA → |
multidrug efflux system |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 2,810,104 | 1 | . | A | 100.0%
| 11.4
/ NA
| 5 | Q8K (CAA→AAA) | emrA | multidrug efflux system |
| Reads supporting (aligned to +/- strand): ref base . (0/0); new base A (0/5); total (0/5) |
CGCAAATGCGGAGACTC‑AAACCCCGCAGCAACCGGTAAAGAAGAGCGGCAAACGTAAGCGTCTG > W3110S.gb/2810088‑2810151
|
cgcAAATGCGGAGACTCAAAACCCCGCAGCAACCGGTAAAGAAGAGCGGCAAACGTAAGCGTCTg < 1:1665949/65‑1 (MQ=255)
cgcAAATGCGGAGACTCAAAACCCCGCAGCAACCGGTAAAGAAGAGCGGCAAACGTAAGCGTCTg < 1:1791053/65‑1 (MQ=255)
cgcAAATGCGGAGACTCAAAACCCCGCAGCAACCGGTAAAGAAGAGCGGCAAACGTAAGCGTCTg < 1:1797274/65‑1 (MQ=255)
cgcAAATGCGGAGACTCAAAACCCCGCAGCAACCGGTAAAGAAGAGCGGCAAACGTAAGCGTCTg < 1:627388/65‑1 (MQ=255)
cgcAAATGCGGAGACTCAAAACCCCGCAGCAACCGGTAAAGAAGAGCGGCAAACGTAAGCGTCTg < 1:922884/65‑1 (MQ=255)
|
CGCAAATGCGGAGACTC‑AAACCCCGCAGCAACCGGTAAAGAAGAGCGGCAAACGTAAGCGTCTG > W3110S.gb/2810088‑2810151
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
