Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F28 I3 R1
|
178 |
99.0 |
1887089 |
95.3% |
1798395 |
63.2 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
3,031,910 |
A→G |
N64D (AAC→GAC) |
idi → |
isopentenyl diphosphate isomerase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 3,031,910 | 0 | A | G | 100.0%
| 24.2
/ NA
| 8 | N64D (AAC→GAC) | idi | isopentenyl diphosphate isomerase |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (0/8); total (0/8) |
ATTAGTTACCCGCCGCGCACTGAGCAAAAAAGCATGGCCTGGCGTGTGGACTAACTCGGTTTGTG > W3110S.gb/3031858‑3031922
|
attaGTTACCCGCCGCGCACTGAGCAAAAAAGCATGGCCTGGCGTGTGGACTGACTCGGTTtgtg < 1:1536106/65‑1 (MQ=255)
attaGTTACCCGCCGCGCACTGAGCAAAAAAGCATGGCCTGGCGTGTGGACTGACTCGGTTtgtg < 1:1632034/65‑1 (MQ=255)
attaGTTACCCGCCGCGCACTGAGCAAAAAAGCATGGCCTGGCGTGTGGACTGACTCGGTTtgtg < 1:1775141/65‑1 (MQ=255)
attaGTTACCCGCCGCGCACTGAGCAAAAAAGCATGGCCTGGCGTGTGGACTGACTCGGTTtgtg < 1:1807891/65‑1 (MQ=255)
attaGTTACCCGCCGCGCACTGAGCAAAAAAGCATGGCCTGGCGTGTGGACTGACTCGGTTtgtg < 1:204249/65‑1 (MQ=255)
attaGTTACCCGCCGCGCACTGAGCAAAAAAGCATGGCCTGGCGTGTGGACTGACTCGGTTtgtg < 1:345127/65‑1 (MQ=255)
attaGTTACCCGCCGCGCACTGAGCAAAAAAGCATGGCCTGGCGTGTGGACTGACTCGGTTtgtg < 1:942756/65‑1 (MQ=255)
ttACCCGCCGCGCACTGAGCAAAAAAGCATGGCCTGGCGTGTGGACTGACTCGGTTtgtg < 1:1798476/60‑1 (MQ=255)
|
ATTAGTTACCCGCCGCGCACTGAGCAAAAAAGCATGGCCTGGCGTGTGGACTAACTCGGTTTGTG > W3110S.gb/3031858‑3031922
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
