Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F28 I1 R1
|
354 |
41.7 |
3110465 |
93.1% |
2895842 |
61.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
940,557 |
A→G |
P236P (CCA→CCG) |
serS → |
seryl‑tRNA synthetase, also charges selenocysteinyl‑tRNA with serine |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 940,557 | 0 | A | G | 100.0%
| 175.0
/ NA
| 47 | P236P (CCA→CCG) | serS | seryl‑tRNA synthetase, also charges selenocysteinyl‑tRNA with serine |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (26/21); total (26/21) |
CGTCCGCTGGAAGAAGAAGCAGACACCAGTAACTATGCGCTGATCCCAACGGCAGAAGTTCCGCTGACTAACCTGGTGCGCGGTGAAATCATCGATGAAGATGATC > W3110S.gb/940510‑940615
|
cGTCCGCTGGAAGAAGAAGCAGACACCAGTAACTATGCGCTGATCCCGACGGC‑‑AAGTTCCGCTg < 1:2774916/64‑1 (MQ=255)
ccGCTGGAAGAAGAAGCAGACACCAGTAACTATGCTCTGATCCCGACGGCAGAAGTTCCGCTg < 1:687580/63‑1 (MQ=255)
ccGCTGGAAGAAGAAGCAGACACCAGTAACTATGCGCTGATCCCGACGGCAGAAGTTCCGCTg < 1:3028211/63‑1 (MQ=255)
ccGCTGGAAGAAGAAGCAGACACCAGTAACTATGCGCTGATCCCGACGGCAGAAGTTCCGCTg < 1:2466854/63‑1 (MQ=255)
ccGCTGGAAGAAGAAGCAGACACCAGTAACTATGCGCTGATCCCGACGGCAGAAGTTCCGCTg < 1:2580880/63‑1 (MQ=255)
ccGCTGGAAGAAGAAGCAGACACCAGTAACTATGCGCTGATCCCGACGGCAGAAGTTCCGCTg < 1:2426011/63‑1 (MQ=255)
ccGCTGGAAGAAGAAGCAGACACCAGTAACTATGCGCTGATCCCGACGGCAGAAGTTCCGCTg < 1:2731602/63‑1 (MQ=255)
ccGCTGGAAGAAGAAGCAGACACCAGTAACTATGCGCTGATCCCGACGGCAGAAGTTCCGCTg < 1:2799095/63‑1 (MQ=255)
ccGCTGGAAGAAGAAGCAGACACCAGTAACTATGCGCTGATCCCGACGGCAGAAGTTCCGCTg < 1:430423/63‑1 (MQ=255)
ccGCTGGAAGAAGAAGCAGACACCAGTAACTATGCGCTGATCCCGACGGCAGAAGTTCCGCTg < 1:1271004/63‑1 (MQ=255)
ccGCTGGAAGAAGAAGCAGACACCAGTAACTATGCGCTGATCCCGACGGCAGAAGTTCCGCTg < 1:852093/63‑1 (MQ=255)
ccGCTGGAAGAAGAAGCAGACACCAGTAACTATGCGCTGATCCCGACGGCAGAAGTTCCGCTg < 1:946907/63‑1 (MQ=255)
ccGCTGGAAGAAGAAGCAGACACCAGAAACTATGCGCTGATCCCGACGGCAGAAGTTCCGCTg < 1:2055252/63‑1 (MQ=255)
ccAGTAACTATGCGCTGATCCCGACGGCAGAAGTTCCGCTg < 1:3041983/41‑1 (MQ=255)
aaCTATGCGCTGATCCCGACGGCAGAAGTTCCGCTg < 1:2395000/36‑1 (MQ=255)
aTGCGCTGATCCCGACGGCAGAAGTTCCGCTGACTAACCTGGTGCGCGGTGAAATCATCGATGaa < 1:1109426/65‑1 (MQ=255)
aTGCGCTGATCCCGACGGCAGAAGTTCCGCTGACTAACCTGGTGCGCGGTGAAATCATCGATGaa < 1:362891/65‑1 (MQ=255)
aTGCGCTGATCCCGACGGCAGAAGTTCCGCTGACTAACCTGGTGCGCGGTGAAATCATCGATGaa < 1:834217/65‑1 (MQ=255)
aTGCGCTGATCCCGACGGCAGAAGTTCCGCTGACTAACCTGGTGCGCGGTGAAATCATCGATGaa < 1:2587255/65‑1 (MQ=255)
aTGCGCTGATCCCGACGGCAGAAGTTCCGCTGACTAACCTGGTGCGCGGTGAAATCATCGATGaa < 1:1359934/65‑1 (MQ=255)
aTGCGCTGATCCCGACGGCAGAAGTTCCGCTGACTAACCTGGTGCGCGGTGAAATCATCGATGaa < 1:2109929/65‑1 (MQ=255)
gATCCCGACGGTAGAAGTTCCGCTGACTAACCTGGTGCGCGGTGAAATCATCGATGAAGATGATc > 1:621938/1‑65 (MQ=255)
gATCCCGACGGCAGAAGTTGCGCTGACTAACCTGGTGCGCGGTGAAATCATCGATGAAGATGATc > 1:1059658/1‑65 (MQ=255)
gATCCCGACGGCAGAAGTTCCGCTGACTAACCTGGTGCGCGGTGAAATCATCGATGAAgatgat > 1:1547637/1‑64 (MQ=255)
gATCCCGACGGCAGAAGTTCCGCTGACTAACCTGGTGCGCGGTGAAATCATCGATGAAGATGATc > 1:3043112/1‑65 (MQ=255)
gATCCCGACGGCAGAAGTTCCGCTGACTAACCTGGTGCGCGGTGAAATCATCGATGAAGATGATc > 1:984454/1‑65 (MQ=255)
gATCCCGACGGCAGAAGTTCCGCTGACTAACCTGGTGCGCGGTGAAATCATCGATGAAGATGATc > 1:89502/1‑65 (MQ=255)
gATCCCGACGGCAGAAGTTCCGCTGACTAACCTGGTGCGCGGTGAAATCATCGATGAAGATGATc > 1:1206753/1‑65 (MQ=255)
gATCCCGACGGCAGAAGTTCCGCTGACTAACCTGGTGCGCGGTGAAATCATCGATGAAGATGATc > 1:738950/1‑65 (MQ=255)
gATCCCGACGGCAGAAGTTCCGCTGACTAACCTGGTGCGCGGTGAAATCATCGATGAAGATGATc > 1:718612/1‑65 (MQ=255)
gATCCCGACGGCAGAAGTTCCGCTGACTAACCTGGTGCGCGGTGAAATCATCGATGAAGATGATc > 1:1370621/1‑65 (MQ=255)
gATCCCGACGGCAGAAGTTCCGCTGACTAACCTGGTGCGCGGTGAAATCATCGATGAAGATGATc > 1:623777/1‑65 (MQ=255)
gATCCCGACGGCAGAAGTTCCGCTGACTAACCTGGTGCGCGGTGAAATCATCGATGAAGATGATc > 1:598276/1‑65 (MQ=255)
gATCCCGACGGCAGAAGTTCCGCTGACTAACCTGGTGCGCGGTGAAATCATCGATGAAGATGATc > 1:1473177/1‑65 (MQ=255)
gATCCCGACGGCAGAAGTTCCGCTGACTAACCTGGTGCGCGGTGAAATCATCGATGAAGATGATc > 1:2458607/1‑65 (MQ=255)
gATCCCGACGGCAGAAGTTCCGCTGACTAACCTGGTGCGCGGTGAAATCATCGATGAAGATGATc > 1:1601220/1‑65 (MQ=255)
gATCCCGACGGCAGAAGTTCCGCTGACTAACCTGGTGCGCGGTGAAATCATCGATGAAGATGATc > 1:3040352/1‑65 (MQ=255)
gATCCCGACGGCAGAAGTTCCGCTGACTAACCTGGTGCGCGGTGAAATCATCGATGAAGATGATc > 1:3031417/1‑65 (MQ=255)
gATCCCGACGGCAGAAGTTCCGCTGACTAACCTGGTGCGCGGTGAAATCATCGATGAAGATGATc > 1:1637822/1‑65 (MQ=255)
gATCCCGACGGCAGAAGTTCCGCTGACTAACCTGGTGCGCGGTGAAATCATCGATGAAGATGATc > 1:2952487/1‑65 (MQ=255)
gATCCCGACGGCAGAAGTTCCGCTGACTAACCTGGTGCGCGGTGAAATCATCGATGAAGATGATc > 1:2920090/1‑65 (MQ=255)
gATCCCGACGGCAGAAGTTCCGCTGACTAACCTGGTGCGCGGTGAAATCATCGATGAAGATGATc > 1:2864743/1‑65 (MQ=255)
gATCCCGACGGCAGAAGTTCCGCTGACTAACCTGGTGCGCGGTGAAATCATCGATGAAGATGATc > 1:2142221/1‑65 (MQ=255)
gATCCCGACGGCAGAAGTTCCGCTGACTAACCTGGTGCGCGGTGAAATCATCGATGAAGATGATc > 1:2144874/1‑65 (MQ=255)
gATCCCGACGGCAGAAGTTCCGCTGACTAACCTGGTGCGCGGTGAAATCATCGATGAAGATGATc > 1:2700938/1‑65 (MQ=255)
gATCCCGACGGCAGAAGTTCCGCTGACTAACCTGGTGCGCGGTGAAATCATCGATGAAGATGATc > 1:2598291/1‑65 (MQ=255)
gATCCCGACGGCAGAAGTTCCGCTGACTAACCTGGTGCGCGGTGAAATCATCGATGAAGATGATc > 1:2390939/1‑65 (MQ=255)
|
CGTCCGCTGGAAGAAGAAGCAGACACCAGTAACTATGCGCTGATCCCAACGGCAGAAGTTCCGCTGACTAACCTGGTGCGCGGTGAAATCATCGATGAAGATGATC > W3110S.gb/940510‑940615
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
