Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F8 I0 R1
|
311 |
50.1 |
4085234 |
95.9% |
3917739 |
60.7 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
W3110S.gb |
940,557 |
A→G |
14.6% |
P236P (CCA→CCG) |
serS → |
seryl‑tRNA synthetase, also charges selenocysteinyl‑tRNA with serine |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 940,557 | 0 | A | G | 14.6%
| 118.5
/ 13.4
| 48 | P236P (CCA→CCG) | serS | seryl‑tRNA synthetase, also charges selenocysteinyl‑tRNA with serine |
| Reads supporting (aligned to +/- strand): ref base A (20/21); new base G (2/5); total (22/26) |
| Fisher's exact test for biased strand distribution p-value = 4.29e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
CTGTTCCATACTCGTCCGCTGGAAGAAGAAGCAGACACCAGTAACTATGCGCTGATCCCAACGGCAGAAGTTCCGCTGACTAACCTGGTGCGCGGTGAAATCATCGATGAAGATGATCTGCCAATTAA > W3110S.gb/940498‑940625
|
cTGTTCCATACTCGTCCGCTGGAAGAAGAAGCAGACACCAGTAACTATGCGCTGATCCCGACGGCAGAAGt > 1:215361/1‑71 (MQ=255)
ttCCATACTCGTCCGCTGGAAGAAGAAGCAGACACCAGTAACTATGCGCTGATCCCGACGGCAGa < 1:1145974/65‑1 (MQ=255)
ttCCATACTCGTCCGCTGGAAGAAGAAGCAGACACCAGTAACTATGCGCTGATCCCAACGGCAGa < 1:1114790/65‑1 (MQ=255)
ttCCATACTCGTCCGCTGGAAGAAGAAGCAGACACCAGTAACTATGCGCTGATCCCAACGGCAGa < 1:302852/65‑1 (MQ=255)
ttCCATACTCGTCCGCTGGAAGAAGAAGCAGACACCAGTAACTATGCGCTGATCCCAACGGCAGa < 1:604674/65‑1 (MQ=255)
ttCCATACTCGTCCGCTGGAAGAAGAAGCAGACACCAGTAACTATGCGCTGATCCCAACGGCAGAAg < 1:1735626/67‑1 (MQ=255)
ccATACTCGTCCGCTGGAAGAAGAAGCAGACACCAGTAACTATGCGCTGATCCCAACGGCAGa < 1:3644259/63‑1 (MQ=255)
ccATACTCGTCCGCTGGAAGAAGAAGCAGACACCAGTAACTATGCGCTGATCCCAACGGCAGAAGTTCCGc > 1:550943/1‑71 (MQ=255)
cATACTCGTCCGCTGGAAGAAGAAGCAGACACCAGTAACTATGCGCTGATCCCAACGGCAGAAGTTCCGCt < 1:2156292/71‑1 (MQ=255)
cTCGTCCGCTGGAAGAAGAAGCAGACACCAGTAACTATGCGCTGATCCCAACGGCAGaa > 1:705876/1‑59 (MQ=255)
cTCGTCCGCTGGAAGAAGAAGCAGACACCAGTAACTATGCGCTGATCCCAACGGCAGAAGTTCCGCTGa > 1:1705939/1‑69 (MQ=255)
cGCTGGAAGAAGAAGCAGACACCAGTAACTATGCGCTGATCCCAACGGCAGAAGTTCCGCTGACTAACCTg < 1:69543/71‑1 (MQ=255)
gaagaagCAGACACCAGTAACTATGCGCTGATCCCAACGGCAGAAGTTCCGCTGACTAACCTGGTGCGCgg > 1:1183979/1‑71 (MQ=255)
aagCAGACACCAGTAACTATGCGCTGATCCCAACGGCAGAAGTTCCGCTGACTAACCt < 1:1547136/58‑1 (MQ=255)
gCAGACACCAGTAACTATGCGCTGATCCCAACGGCAGAAGTTCCGCTGACTAACCTGGTGCGCGGTGAAat > 1:3538923/1‑71 (MQ=255)
gACACCAGTAACTATGCGCTGATCCCAACGGCAGAAGtt > 1:1244965/1‑39 (MQ=255)
gACACCAGTAACTATGCGCTGATCCCAACGGCAGAAGTTCCGCTGACTAACCTGGTGCGCGGTGAAatcat > 1:1524440/1‑71 (MQ=255)
cacCAGTAACTATGCGCTGATCCCAACGGCAGAAGTTCCGCTGACTAACCTGGTGCGCGGTGAAATCATCg < 1:896460/71‑1 (MQ=255)
cacCAGTAACTATGCGCTGATCCCAACGGCAGAAGTTCCGCTGACTAACCTGGTGCGCGGTGAAATCATCg < 1:931813/71‑1 (MQ=255)
acCAGTAACTATGCGCTGATCCCGACGGCAGAAGTTCCGCTGACTAACCTGGTGCGCGGTGAAATCATCGa > 1:2347720/1‑71 (MQ=255)
cAGTAACTATGCGCTGATCCCAACGGCAGAAGTTCCGCTGACTAACCTGGTGCGCGGTGAAATCATCGAt > 1:3849752/1‑70 (MQ=255)
cAGTAACTATGCGCTGATCCCAACGGCAGAAGTTCCGCTGACTAACCTGGTGCGCGGTGAAATCATCGATg < 1:1689290/71‑1 (MQ=255)
aGTAACTATGCGCTGATCCCAACGGCAGAAGTTCCGc > 1:4016986/1‑37 (MQ=255)
aGTAACTATGCGCTGATCCCAACGGCAGAAGTTCCGc > 1:293511/1‑37 (MQ=255)
aGTAACTATGCGCTGATCCCAACGGCAGAAGTTCCGc > 1:2625415/1‑37 (MQ=255)
aGTAACTATGCGCTGATCCCAACGGCAGAAGTTCCGc > 1:2620765/1‑37 (MQ=255)
aGTAACTATGCGCTGATCACAACGGCAGAAGTTCCGc > 1:2921013/1‑37 (MQ=255)
gTAACTATGCGCTGATCCCAACGGCAGAAGTTCCGCTGACTAACCt > 1:1831341/1‑46 (MQ=255)
tAACTATGCGCTGATCCCGACTGCAGAAGTTCCGCTGACTAACCTGGTGCGCGGTGAAat < 1:3123389/60‑1 (MQ=255)
aCTATGCGCTGATCCCAACGGCAGAAGTTCCGCTGACTAACCTGGTGCGCGGTGAAATCATCGATGAAgat > 1:2170742/1‑71 (MQ=255)
aCTATGCGCTGATCCCAACGGCAGAAGTTCCGCTGACTAACCTGGTGCGCGGTGAAATCATCGATGAAga > 1:491005/1‑70 (MQ=255)
cTATGCGCTGATCCCGACGGCAGAAGTTCCGCTGACTAACCTGGTGCGCGGTGa < 1:2792783/54‑1 (MQ=255)
cTATGCGCTGATCCCGACGGCAGAAGTTCCGCTGACTAACCTGGTGCGCGGTGa < 1:775885/54‑1 (MQ=255)
cTATGCGCTGATCCCGACGGCAGAAGTTCCGCTGACTAACCTGGTGCGCGGTGa < 1:2017663/54‑1 (MQ=255)
cTATGCGCTGATCCCAACGTCAGAAGTTCCGCTGACTAACCTGGTGCGCGGTGAAATCATCGATGAAgatg < 1:2206087/71‑1 (MQ=255)
cTATGCGCTGATCCCAACGGCAGAAGTTCCGCTGACTAACCTGGTGCGCGGTGAAATCATCGATGAAgatg < 1:2722016/71‑1 (MQ=255)
tGCGCTGATCCCAACGGCAGAAGTTCCGCTGACTAAcc < 1:1023324/38‑1 (MQ=255)
tGCGCTGATCCCAACGGCAGAAGTTCCGCTGACTAAcc < 1:3305164/38‑1 (MQ=255)
tGCGCTGATCCCAACGGCAGAAGTTCCGCTGACTAACCTGGTGCGCGGTGAAATCATCGATGAAgatga < 1:601422/69‑1 (MQ=255)
tGCGCTGATCCCAACGGCAGAAGTTCCGCTGACTAACCTGGTGCGCGGTGAAATCATCGATGAAGATGATc > 1:3880736/1‑71 (MQ=255)
tGCGCTGATCCCAACGGCAGAAGTTCCGCTGACTAACCTGGTGCGCGGTGAAATCATCGATGAAGATGATc > 1:12865/1‑71 (MQ=255)
cgcTGATCCCAACGGCAGAAGTTCCGCTGACTAACCTg > 1:3671475/1‑38 (MQ=255)
aTCCCAACGTCAGAAGTTCCGCTGACTAACCTGGTGCGCGGTGAAatcatc < 1:1320358/51‑1 (MQ=255)
aTCCCAACGGCAGAAGTTCCGCTGACTAACCTGGTGCGCGGTGAAatcatc < 1:3636536/51‑1 (MQ=255)
cccAACGGCAGAAGTTCCGCTGACTAACCTGGTGCGCGGTGAAATCATCGATGAAGATGATCTGCCAAtt > 1:1893965/1‑70 (MQ=255)
ccAACGGCAGAAGTTCCGCTGACTAACCTGGTGCGCGGTGAAATCATCGATGAAGATGATCTGCGAATTaa < 1:8204/71‑1 (MQ=255)
ccAACGGCAGAAGTTCCGCTGACTAACCTGGTGCGCGGTGAAATCATCGATGAAGATGATCTGCCAATTaa < 1:2284597/71‑1 (MQ=255)
ccAACGCCAGAAGTTCCGCTGACTAACCTGGTGCGCGGTGAAATCATCGATGAAGATGATCTGCCAATTaa < 1:628535/71‑1 (MQ=255)
|
CTGTTCCATACTCGTCCGCTGGAAGAAGAAGCAGACACCAGTAACTATGCGCTGATCCCAACGGCAGAAGTTCCGCTGACTAACCTGGTGCGCGGTGAAATCATCGATGAAGATGATCTGCCAATTAA > W3110S.gb/940498‑940625
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
